Choline Acetyltransferase/ChAT Antibody

Images

 
Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brainstem showing specific labeling using the anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.

Product Details

Summary
Reactivity Hu, Mu, Rt, Av, Ch, GP, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Choline Acetyltransferase/ChAT Antibody Summary

Immunogen
Native choline acetyltransferase purifed from human placenta
Marker
Cholinergic Neuronal Marker
Specificity
Specific for the ~ 70k choline acetyltransferase protein
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CHAT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunohistochemistry Whole-Mount
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 21912682) Use in Immunohistochemistry-whole mount reported in scientific literature (PMID 24630395). ICC/IF reported in scientific literature (PMID 23095258).
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Choline Acetyltransferase/ChAT Lysate (NBP2-66358)
Publications
Read Publications using
NBP1-30052 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 5 mg/ml BSA
Preservative
0.2% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Choline Acetyltransferase/ChAT Antibody

  • acetyl CoA:choline O-acetyltransferase
  • CHAT
  • CHOACTase
  • Choline acetylase
  • Choline Acetyltransferase
  • choline O-acetyltransferase
  • CMS1A
  • CMS1A2
  • EC 2.3.1
  • EC 2.3.1.6

Background

Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.; Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.; CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine.; Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Choline Acetyltransferase/ChAT Antibody (NBP1-30052)(7)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-WhMt.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(3)
IHC-WhMt
(1)
All Applications
Filter By Species
Mouse
(6)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-30052 Applications Species
Burstein SR, Valsecchi F, Kawamata H et al. In vitro and in vivo studies of the ALS-FTLD protein CHCHD10 reveal novel mitochondrial topology and protein interactions Hum. Mol. Genet. 2017 Nov 03 [PMID: 29112723] (Mouse) Mouse
Gelfo F, Cutuli D, Nobili A et al. Chronic Lithium Treatment in a Rat Model of Basal Forebrain Cholinergic Depletion: Effects on Memory Impairment and Neurodegeneration. J. Alzheimers Dis. 2017 Feb 22 [PMID: 28222508]
Zhang GQ, Yang S, Li XS, Zhou DS. Expression and possible role of IGF-IR in the mouse gastric myenteric plexus and smooth muscles. Acta Histochem. 3/12/2014 [PMID: 24630395] (IHC-Fr, IHC-WhMt, Mouse) IHC-Fr, IHC-WhMt Mouse
Martella G, Maltese M, Nistico R et al. Regional specificity of synaptic plasticity deficits in a knock-in mouse model of DYT1 dystonia. Neurobiol. Dis. 2014 Mar 3 [PMID: 24503369] (IHC-Fr, Mouse) IHC-Fr Mouse
Ponterio G, Tassone A, Sciamanna G et al. Powerful inhibitory action of mu opioid receptors (MOR) on cholinergic interneuron excitability in the dorsal striatum. Neuropharmacology 2013 Jul 25 [PMID: 23891638] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Liu J, Tu H, Zhang D et al. Voltage-gated sodium channel expression and action potential generation in differentiated NG108-15 cells BMC Neurosci 2012 Oct 25 [PMID: 23095258] (ICC/IF, Mouse, Rat) ICC/IF Mouse, Rat
Sciamanna G, Tassone A, Martella G et al. Developmental Profile of the Aberrant Dopamine D2 Receptor Response in Striatal Cholinergic Interneurons in DYT1 Dystonia. PLoS ONE;6(9). 2011 [PMID: 21912682] (IHC-Fr, Mouse) IHC-Fr Mouse

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Choline Acetyltransferase/ChAT Antibody (NBP1-30052) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Choline Acetyltransferase/ChAT Antibody (NBP1-30052)

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Diseases for Choline Acetyltransferase/ChAT Antibody (NBP1-30052)

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Blogs on Choline Acetyltransferase/ChAT.

Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons
Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group...  Read full blog post.

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Bioinformatics

Gene Symbol CHAT
Entrez
Uniprot