Choline Acetyltransferase/ChAT Antibody


Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brainstem showing specific labeling using the anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.

Product Details

Reactivity Hu, Mu, Rt, Ch, Gp, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl, IHC-WhMt

Order Details

Choline Acetyltransferase/ChAT Antibody Summary

Native choline acetyltransferase purifed from human placenta
Cholinergic Neuronal Marker
Specific for the ~ 70k choline acetyltransferase protein
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 21912682) Use in Immunohistochemistry-whole mount reported in scientific literature (PMID 24630395). ICC/IF reported in scientific literature (PMID 23095258). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 30261285).
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Choline Acetyltransferase/ChAT Knockout HeLa Cell Lysate
Read Publications using
NBP1-30052 in the following applications:

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS and 5 mg/ml BSA
0.2% Sodium Azide
Immunogen affinity purified

Alternate Names for Choline Acetyltransferase/ChAT Antibody

  • acetyl CoA:choline O-acetyltransferase
  • CHAT
  • CHOACTase
  • Choline acetylase
  • Choline Acetyltransferase
  • choline O-acetyltransferase
  • CMS1A
  • CMS1A2
  • EC 2.3.1
  • EC


Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.; Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.; CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine.; Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Choline Acetyltransferase/ChAT Antibody (NBP1-30052)(14)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-WhMt, WB.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-30052 Applications Species
Bonsi P, Ponterio G, Vanni V et al. RGS9-2 rescues dopamine D2 receptor levels and signaling in DYT1 dystonia mouse models EMBO Mol Med Dec 14 2018 [PMID: 30552094] (ICC/IF, Mouse) ICC/IF Mouse
Sun T, Li D, Hu S et al. Aging-dependent decrease in the numbers of enteric neurons, interstitial cells of Cajal and expression of connexin43 in various regions of gastrointestinal tract. Aging (Albany NY) Dec 11 2018 [PMID: 30530917] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Mellone M, Zianni E, Stanic J et al. NMDA receptor GluN2D subunit participates to levodopa-induced dyskinesia pathophysiology. Neurobiol. Dis. Sep 24 2018 [PMID: 30261285] (IHC-FrFl, Rat) IHC-FrFl Rat
Palomo GM, Granatiero V, Kawamata H et al. Parkin is a disease modifier in the mutant SOD1 mouse model of ALS EMBO Mol Med Aug 20 2018 [PMID: 30126943] (IHC, Mouse) IHC Mouse
Simandi Z, Pajer K, Karolyi K et al. Arginine Methyltransferase PRMT8 Provides Cellular Stress Tolerance in Aging Motoneurons J. Neurosci. Aug 29 2018 [PMID: 30054395] (WB, Mouse) WB Mouse
Rotheneichner P, Belles M, Benedetti B et al. Cellular Plasticity in the Adult Murine Piriform Cortex: Continuous Maturation of Dormant Precursors Into Excitatory Neurons Cereb. Cortex 2018 Apr 21 [PMID: 29688272] (IHC, Mouse) IHC Mouse
Burstein SR, Valsecchi F, Kawamata H et al. In vitro and in vivo studies of the ALS-FTLD protein CHCHD10 reveal novel mitochondrial topology and protein interactions Hum. Mol. Genet. 2017 Nov 03 [PMID: 29112723] (Mouse) Mouse
Stanic J, Mellone M, Napolitano F et al. Rabphilin 3A: A novel target for the treatment of levodopa-induced dyskinesias Neurobiol. Dis. 2017 Aug 18 [PMID: 28823933] (IHC, Rat) IHC Rat
Gelfo F, Cutuli D, Nobili A et al. Chronic Lithium Treatment in a Rat Model of Basal Forebrain Cholinergic Depletion: Effects on Memory Impairment and Neurodegeneration. J. Alzheimers Dis. 2017 Feb 22 [PMID: 28222508]
Zhang GQ, Yang S, Li XS, Zhou DS. Expression and possible role of IGF-IR in the mouse gastric myenteric plexus and smooth muscles. Acta Histochem. 3/12/2014 [PMID: 24630395] (IHC-Fr, IHC-WhMt, Mouse) IHC-Fr, IHC-WhMt Mouse
Show All 14 Publications.

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Product General Protocols

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Blogs on Choline Acetyltransferase/ChAT.

Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons
Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group...  Read full blog post.

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