Choline Acetyltransferase/ChAT Antibody


Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - The decrease in protein expressions of c-kit, Choline Acetyltransferase/ChAT, nNOS and Cx43 in the colon of elderly humans. Expressions of c-kit, more
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Representative images of lumbar spinal cord sections at 130 days immunostained with Choline Acetyltransferase/ChAT. Scale more
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brainstem showing specific labeling using the anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.
Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Immunostaining of anterior horn of lumbar spinal cord for Choline Acetyltransferase/ChAT (in green) and p62 (in red) at 130 more

Product Details

Reactivity Hu, Mu, Rt, Ch, Gp, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC

Order Details

Choline Acetyltransferase/ChAT Antibody Summary

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Native choline acetyltransferase purifed from human placenta
Cholinergic Neuronal Marker
Specific for endogenous levels of the ~70 kDa Choline Acetyltransferase/ChAT protein.
Affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 21912682) Use in Immunohistochemistry-whole mount reported in scientific literature (PMID 24630395). ICC/IF reported in scientific literature (PMID 23095258). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 30261285).
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP1-30052 in the following applications:

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS and 5 mg/ml BSA
0.2% Sodium Azide
Affinity purified

Alternate Names for Choline Acetyltransferase/ChAT Antibody

  • acetyl CoA:choline O-acetyltransferase
  • CHAT
  • CHOACTase
  • Choline acetylase
  • Choline Acetyltransferase
  • choline O-acetyltransferase
  • CMS1A
  • CMS1A2
  • EC 2.3.1
  • EC


Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.; Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.; CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine.; Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Choline Acetyltransferase/ChAT Antibody (NBP1-30052)(22)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 8 applications: EM, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-FrFl, IHC-WhMt, WB.

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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NBP1-30052 Applications Species
Liu S, Xiang K, Yuan F, Xiang M Generation of self-organized autonomic ganglion organoids from fibroblasts iScience 2023-03-01 [PMID: 36922996]
Wang J, Xu L, Peng D et al. IFN-?-STAT1-mediated CD8+ T-cell-neural stem cell cross talk controls astrogliogenesis after spinal cord injury Inflammation and regeneration 2023-02-13 [PMID: 36782279] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
You J, Maksimovic K, Lee J et al. Selective Loss of MATR3 in Spinal Interneurons, Upper Motor Neurons and Hippocampal CA1 Neurons in a MATR3 S85C Knock-In Mouse Model of Amyotrophic Lateral Sclerosis Biology 2022-02-12 [PMID: 35205163] (IF/IHC, Mouse) IF/IHC Mouse
Radzimirska M, Kuchinka J, Kuder T Et al. Distribution and neurochemical characteristic of the cardiac nerve structures in the heart of chinchilla (Chinchilla laniger Molina) Folia histochemica et cytobiologica 2021-09-28 [PMID: 34581422]
Tassone A, Martella G, Meringolo M et al. Vesicular Acetylcholine Transporter Alters Cholinergic Tone and Synaptic Plasticity in DYT1 Dystonia Movement disorders : official journal of the Movement Disorder Society 2021-06-26 [PMID: 34173686]
Li Z, Wang Y, Jiang Y et al. Xiao-Qing-Long-Tang Maintains Cardiac Function during Heart Failure with Reduced Ejection Fraction in Salt-Sensitive Rats by Regulating the Imbalance of Cardiac Sympathetic Innervation Evidence-Based Complementary and Alternative Medicine 2020-11-24 [PMID: 33628295] (EM) EM
Yao Y, Wang J, He T et al. Microarray assay of circular RNAs reveals cicRNA.7079 as a new anti-apoptotic molecule in spinal cord injury in mice Brain Res Bull 2020-09-04 [PMID: 32882320]
Radzimirska M, Kuchinka J, Nowak E et al. Cholinergic and adrenergic innervation of the pancreas in chinchilla (Chinchilla Laniger Molina) Folia Histochemica et Cytobiologica 2020-04-09 [PMID: 32202307] (IHC) IHC
Bonsi P, Ponterio G, Vanni V et al. RGS9-2 rescues dopamine D2 receptor levels and signaling in DYT1 dystonia mouse models EMBO Mol Med 2018-12-14 [PMID: 30552094] (ICC/IF, Mouse) ICC/IF Mouse
Sun T, Li D, Hu S et al. Aging-dependent decrease in the numbers of enteric neurons, interstitial cells of Cajal and expression of connexin43 in various regions of gastrointestinal tract. Aging (Albany NY) 2018-12-11 [PMID: 30530917] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Show All 22 Publications.

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Product General Protocols

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Blogs on Choline Acetyltransferase/ChAT.

Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons
Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group ...  Read full blog post.

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Gene Symbol CHAT