14-3-3 Antibody

Images

 
Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed ...read more
Immunocytochemistry/ Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

14-3-3 Antibody Summary

Immunogen
A portion of amino acids 125-175 from human 14-3-3 was used as the immunogen for this antibody.
Predicted Species
Monkey (100%), Reptile (94%), Zebrafish (94%), Amphibian (100%), Bovine (94%), Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YWHAQ
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 2-4ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 2-5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. Simple Western reported in a verified customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-27202 in the following applications:

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in Frog, Crab-eating Macaque.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for 14-3-3 Antibody

  • 14-3-3 protein tau
  • 14-3-3 protein T-cell
  • 14-3-3 protein theta
  • 1433
  • 14-3-3
  • 14-3-3,1C5
  • HS1
  • Protein HS1
  • protein tau
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide

Background

The 14-3-3 family of proteins are important regulatory molecules ubiquitiously expressed in all eukarryotic cells which bind to numerous signaling proteins in various pathways driving critical cellular pathways of apoptosis, differentiation and cell cycle (1-3). 14-3-3 theta specifically has also been shown to play an important regulatory role in the TLR2 signaling pathways (1) as a negative regulator of TLR2 ligand Pam3CySk4 induced NF-kB activation. 14-3-3 theta has previously been shown to interact with TLR4 ligand and MyD88 dependent phosphorylated PkC epsilon (3). 14-3-3 theta in the TLR4 signaling pathway is a positive regulator controlling release of IRF3 indcuded pro-inflammatory cytokines RANTES and IP-10 (3, 4). Currently identified by mass spec as part of the TLR2 signaling complex (1) and taken along with TLR4 data, a 14-3-3 theta antibody can be used to examine the different regulatory functions of 14-3-3 theta for different TLRs through its interaction with common or unique TLR signaling adaptor molecules in addition to MyD88 or PkC epsilon such as TRAM or TRIF allowing further clarification of TLR specific pathway regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF
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Review for 14-3-3 Antibody (NBP2-27202) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-27202:
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Simple Western
(1)
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Images Ratings Applications Species Date Details
Simple Western 14-3-3 NBP2-27202
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reviewed by:
Anonymous
Simple Western Mouse 01/21/2016
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Summary

ApplicationSimple Western
Sample Testedwhole brain homogenates from mice
SpeciesMouse

Blocking

Blocking Detailsn/a

Primary Anitbody

Dilution Ratio1:500, 1:100, 1:50

Details

Detection NotesWes Simple Western platform

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 14-3-3 Antibody (NBP2-27202) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional 14-3-3 Products

Array NBP2-27202

Bioinformatics Tool for 14-3-3 Antibody (NBP2-27202)

Discover related pathways, diseases and genes to 14-3-3 Antibody (NBP2-27202). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for 14-3-3 Antibody (NBP2-27202)

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Pathways for 14-3-3 Antibody (NBP2-27202)

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Research Areas for 14-3-3 Antibody (NBP2-27202)

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Blogs on 14-3-3.

Role of RASSF1A in Death Receptor-Dependent Apoptosis
Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and t...  Read full blog post.

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Recent Reviews

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1
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Anonymous
01/21/2016
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol YWHAQ
Uniprot