Western blot shows lysates of mouse pituitary tissue and rat pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) ...read more
Recombinant Mouse Prolactin (Catalog # 1445-PL) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Prolactin (10 ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Prolactin Antibody [Unconjugated]
Prolactin (PRL) is a neuroendocrine pituitary hormone. Prolactin is synthesized by the anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, NK cells and breast cancer cells. Originally characterized as a lactogenic hormone, further studies have demonstrated broader roles in breast cancer development, regulation of reproductive function, and immunoregulation. In the immune system, Prolactin has been shown to be secreted by human PBMC and to act as a proliferative growth factor. Additionally, Prolactin treatment of human PBMC has been shown to enhance IFN-gamma production. In the breast, Prolactin-induced morphogenesis of the mammary cells is mediated through IGF-2, which in turn upregulates cyclin D1. Prolactin has several molecular forms. The predominant form is a monomer; the non-glycosylated form is 23 kDa and the glycosylated form is 25 kDa. Glycosylated Prolactin is removed from the circulation faster and has been reported to have lower biological potency. Mouse Prolactin cDNA encodes a 228 amino acid (aa) residue protein with a putative 31 aa residue signal peptide. The Prolactin receptor is a transmembrane type I glycoprotein that belongs to the cytokine hematopoietic receptor family. B cells, T cells, macrophages, NK cells, monocytes, CD34+ progenitor cells, neutrophils, mammary gland, liver, kidney, adrenals, ovaries, testis, prostrate, seminal vesicles, and hypothalamus have all been shown to express the Prolactin receptor. Three forms of the receptor, generated by differential splicing, have been identified. These isoforms differ in the length of their cytoplasmic domains. It is believed that the short cytoplasmic form is non-functional. Prolactin signal transduction involves the JAK/STAT families and Src kinase family (1‑9).
Freeman, M. et al. (2000) Physiological Reviews 80:1523.
Ben-Johnson, N. et al. (1996) Endoc. Rev. 17:639.
Cesario, T. et al. (1994) Proc. Soc. Exp. Biol. Med. 205:89.
Price, A.E. et al. (1995) Endoc. 136:4827.
Hoffmann, T. et al. (1993) J. Endoc. Invest. 16:807.
Cole, E. et al. (1991) Endoc. 129:2639.
Lewis, U. et al. (1985) Endoc. 116:359.
Matalk, K. (2003) Cytokine 21:187.
Brisken, C. et al. (2002) Dev. Cell 3:877.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Prolactin Antibody [Unconjugated] and receive a gift card or discount.