Prolactin Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse pituitary tissue and rat pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) ...read more
Recombinant Mouse Prolactin (Catalog # 1445-PL) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Rec­ombinant Mouse Prolactin (10 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Prolactin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Prolactin
Leu32-Cys228
Accession # NP_035294
Specificity
Detects mouse and rat Prolactin in Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Prolactin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PRL
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.25-1.0 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse Prolactin Antibody (Catalog # AF1445)
ELISA Detection: Mouse Prolactin Biotinylated Antibody (Catalog # BAF1445)
Standard: Recombinant Mouse Prolactin (Catalog # 1445-PL)
Publications
Read Publications using
AF1445 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prolactin Antibody [Unconjugated]

  • PRL
  • Prolactin

Background

Prolactin (PRL) is a neuroendocrine pituitary hormone. Prolactin is synthesized by the anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, NK cells and breast cancer cells. Originally characterized as a lactogenic hormone, further studies have demonstrated broader roles in breast cancer development, regulation of reproductive function, and immunoregulation. In the immune system, Prolactin has been shown to be secreted by human PBMC and to act as a proliferative growth factor. Additionally, Prolactin treatment of human PBMC has been shown to enhance IFN-gamma production. In the breast, Prolactin-induced morphogenesis of the mammary cells is mediated through IGF-2, which in turn upregulates cyclin D1. Prolactin has several molecular forms. The predominant form is a monomer; the non-glycosylated form is 23 kDa and the glycosylated form is 25 kDa. Glycosylated Prolactin is removed from the circulation faster and has been reported to have lower biological potency. Mouse Prolactin cDNA encodes a 228 amino acid (aa) residue protein with a putative 31 aa residue signal peptide. The Prolactin receptor is a transmembrane type I glycoprotein that belongs to the cytokine hematopoietic receptor family. B cells, T cells, macrophages, NK cells, monocytes, CD34+ progenitor cells, neutrophils, mammary gland, liver, kidney, adrenals, ovaries, testis, prostrate, seminal vesicles, and hypothalamus have all been shown to express the Prolactin receptor. Three forms of the receptor, generated by differential splicing, have been identified. These isoforms differ in the length of their cytoplasmic domains. It is believed that the short cytoplasmic form is non-functional. Prolactin signal transduction involves the JAK/STAT families and Src kinase family (1‑9).

  1. Freeman, M. et al. (2000) Physiological Reviews 80:1523.
  2. Ben-Johnson, N. et al. (1996) Endoc. Rev. 17:639.
  3. Cesario, T. et al. (1994) Proc. Soc. Exp. Biol. Med. 205:89.
  4. Price, A.E. et al. (1995) Endoc. 136:4827.
  5. Hoffmann, T. et al. (1993) J. Endoc. Invest. 16:807.
  6. Cole, E. et al. (1991) Endoc. 129:2639.
  7. Lewis, U. et al. (1985) Endoc. 116:359.
  8. Matalk, K. (2003) Cytokine 21:187.
  9. Brisken, C. et al. (2002) Dev. Cell 3:877.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prolactin Antibody (AF1445)(5)

We have publications tested in 2 confirmed species: Mouse, Gecko.

We have publications tested in 5 applications: Bioassay, IF, IHC-Fr, IHC-P, Neutralization.


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Bioassay
(1)
IF
(1)
IHC-Fr
(1)
IHC-P
(1)
Neutralization
(1)
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Mouse
(4)
Gecko
(1)
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Showing Publications 1 - 5 of 5.
Publications using AF1445 Applications Species
H Suzuki, T Yamamoto Chemokine CXCL14-like immunoreactivity in the &amp;alphaMSH-producing cells and PRL-producing cells of the flat-tailed house gecko pituitary J. Vet. Med. Sci., 2020;82(4):408-413. 2020 [PMID: 32037367] (IF, Gecko) IF Gecko
K Handa, S Kiyohara, T Yamakawa, K Ishikawa, M Hosonuma, N Sakai, A Karakawa, M Chatani, M Tsuji, K Inagaki, Y Kiuchi, M Takami, T Negishi-Ko Bone loss caused by dopaminergic degeneration and levodopa treatment in Parkinson&#039;s disease model mice Sci Rep, 2019;9(1):13768. 2019 [PMID: 31551490] (Bioassay, Mouse) Bioassay Mouse
Christensen, Heather, Murawsky, Michael, Horseman, Nelson D, Willson, Tara A, Gregerson, Karen A Completely humanizing prolactin rescues infertility in prolactin knockout mice and leads to human prolactin expression in extrapituitary mouse tissues. Endocrinology, 2013;154(12):4777-89. 2013 [PMID: 24029242] (IHC-Fr, Mouse) IHC-Fr Mouse
Mak GK, Weiss S, Paternal recognition of adult offspring mediated by newly generated CNS neurons. Nat. Neurosci., 2010;13(6):753-8. 2010 [PMID: 20453850] (Neutralization, Mouse) Neutralization Mouse
Korsisaari N, Ross J, Wu X, Kowanetz M, Pal N, Hall L, Eastham-Anderson J, Forrest WF, van Bruggen N, Peale FV, Ferrara N Blocking vascular endothelial growth factor-a inhibits the growth of pituitary adenomas and lowers serum prolactin level in a mouse model of multiple endocrine neoplasia type 1. Clin. Cancer Res., 2008;14(1):249-58. 2008 [PMID: 18172277] (IHC-P, Mouse) IHC-P Mouse

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1445

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Bioinformatics

Gene Symbol PRL
Uniprot