SLC6A3/DAT1 Antibody (mAb16)


Western Blot: DAT1 Antibody (mAb16) [NBP2-22164] - Analysis of DAT1 expression in rat striatal membrane tissue lysate.
Immunocytochemistry/ Immunofluorescence: DAT1 Antibody (mAb16) [NBP2-22164] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with more
Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.

Product Details

Reactivity Mu, Rt, Hu(-)Species Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

SLC6A3/DAT1 Antibody (mAb16) Summary

Synthetic peptide corresponding to the N-terminus of rat DAT1. [UniProt# P23977]
Membrane; Multi-pass membrane protein.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
Application Notes
This DAT1 (mAb16) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. In WB a band can been seen at ~70-85 kDa. Use in Immunoprecipitation reported in scientific literature (PMID: 20643191).
Reviewed Applications
Read 1 Review rated 4
NBP2-22164 in the following applications:

Read Publications using
NBP2-22164 in the following applications:

  • IP
    1 publication
  • WB
    11 publications

Reactivity Notes

Mouse and rat. Little to no human reactivity has been observed.

Alternate Names for SLC6A3/DAT1 Antibody (mAb16)

  • DA transporter
  • DAT1
  • DATDAT1Solute carrier family 6 member 3
  • SLC6A3
  • sodium-dependent dopamine transporter
  • solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
  • VNTR


DAT1, also known as SLC6A3 and Sodium-dependent dopamine transporter, is a sodium and chloride-dependent dopamine transporter belonging to the solute carrier family 6. Dopamine transporters are distributed widely across the brain in regions with high dopaminergic activity, such as the striatum and substantia nigra. These transporters effectively provide fast acting clearance of dopamine, adrenalin and noradrenalin from the synaptic cleft, thus terminating the neurotransmitter signal. DATs have been found to interact with PRKCABP, TGFB1I1, SLC18A2 and the N-terminus of SYNGR3. DATs are also the target proteins for psychomotor stimulants such as amphetamines and cocaine. Research has implicated DATs as useful markers in detecting depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Mu, Rt, Hu(-)
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Applications: WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, In vitro, RIA

Publications for SLC6A3/DAT1 Antibody (NBP2-22164)(11)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IP, WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-22164 Applications Species
Moritz AE, Foster JD, Gorentla BK et al. Phosphorylation of dopamine transporter serine 7 modulates cocaine analog binding. J Biol Chem 2013 Jan 4 [PMID:23161550] (WB, Rat) WB Rat
Foster JD, Yang JW, Moritz AE et al. Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux. J Biol Chem 2012 Aug 24 [PMID:22722938] (WB, Rat, Mouse) WB Rat, Mouse
Foster JD, Vaughan RA. Palmitoylation controls dopamine transporter kinetics, degradation, and protein kinase C-dependent regulation. J Biol Chem 2011 Feb 18 [PMID:21118819] (WB, Rat) WB Rat
Cervinski MA, Foster JD, Vaughan RA. Syntaxin 1A regulates dopamine transporter activity, phosphorylation and surface expression. Neuroscience 2010 Oct 13 [PMID:20643191] (WB, IP, Rat) WB, IP Rat
Perry ML, Leinninger GM, Chen R et al. Leptin promotes dopamine transporter and tyrosine hydroxylase activity in the nucleus accumbens of Sprague-Dawley rats. J Neurochem 2010 Aug [PMID:20412389] (WB, Rat) WB Rat
Furman CA, Chen R, Guptaroy B et al. Dopamine and amphetamine rapidly increase dopamine transporter trafficking to the surface: live-cell imaging using total internal reflection fluorescence microscopy. J Neurosci 2009 Mar 11 [PMID:19279270] (WB, Rat) WB Rat
Gorentla BK, Moritz AE, Foster JD, Vaughan RA. Proline-directed phosphorylation of the dopamine transporter N-terminal domain. Biochemistry 2009 Feb 10 [PMID:19146407] (WB, Rat) WB Rat
Chen R, Zhang M, Park S, Gnegy ME. C57BL/6J mice show greater amphetamine-induced locomotor activation and dopamine efflux in the striatum than 129S2/SvHsd mice. Pharmacol Biochem Behav 2007 May [PMID:17524461] (WB, Mouse) WB Mouse
Johnson LA, Furman CA, Zhang M et al. Rapid delivery of the dopamine transporter to the plasmalemmal membrane upon amphetamine stimulation. Neuropharmacology 2005 Nov [PMID:16212991] (WB, Rat) WB Rat
Johnson LA, Guptaroy B, Lund D et al. Regulation of amphetamine-stimulated dopamine efflux by protein kinase C beta. J Biol Chem 2005 Mar 25 [PMID:15647254] (WB, Rat) WB Rat
Show All 11 Publications.

Reviews for SLC6A3/DAT1 Antibody (NBP2-22164) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-22164:
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Images Ratings Applications Species Date Details
Western Blot SLC6A3/DAT1 NBP2-22164
reviewed by:
WB Mouse 04/08/2016


ApplicationWestern Blot
Sample Testedmouse brain


Blocking DetailsOdyssey (PBS) blocking buffer (Licor)

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG-IRDye 800CW
Secondary Manufacturer Cat#926-32210
Secondary Concentration1:15,000


Detection NotesLicor Odyssey CLx

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC6A3/DAT1 Antibody (NBP2-22164). (Showing 1 - 1 of 1 FAQs).

  1. Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
    • Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP2-22164AF405
Alexa Fluor (R) 488 Labeled NBP2-22164AF488
Alexa Fluor (R) 647 Labeled NBP2-22164AF647
Alexa Fluor (R) 700 Labeled NBP2-22164AF700
Allophycocyanin Labeled NBP2-22164APC
DyLight 350 Labeled NBP2-22164UV
DyLight 405 Labeled NBP2-22164V
DyLight 405LS Labeled NBP2-22164V3
DyLight 488 Labeled NBP2-22164G
DyLight 680 Labeled NBP2-22164FR
DyLight 755 Labeled NBP2-22164IR
PE Labeled NBP2-22164PE
PerCP Labeled NBP2-22164PCP

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Contact Information

Product PDFs


Gene Symbol SLC6A3

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