Home » SLC6A3/DAT1 » SLC6A3/DAT1 Antibodies » SLC6A3/DAT1 Antibody (mAb16)

SLC6A3/DAT1 Antibody (mAb16)

 (12 Publications)
 (3 Reviews)
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Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.
Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.
Immunocytochemistry/ Immunofluorescence: DAT1 Antibody (mAb16) [NBP2-22164] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with ...read more
Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.
Western Blot: DAT1 Antibody (mAb16) [NBP2-22164] - Analysis of DAT1 expression in rat striatal membrane tissue lysate.

Product Details

Summary
Reactivity Mu, Rt, Hu(-)Species Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
mAb16
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22164AF350
Alexa Fluor 405
NBP2-22164AF405
Alexa Fluor 488
NBP2-22164AF488
Alexa Fluor 532
NBP2-22164AF532
Alexa Fluor 594
NBP2-22164AF594
Alexa Fluor 647
NBP2-22164AF647
Alexa Fluor 700
NBP2-22164AF700
Alexa Fluor 750
NBP2-22164AF750
Biotin
NBP2-22164B
DyLight 350
NBP2-22164UV
DyLight 405
NBP2-22164V
DyLight 488
NBP2-22164G
DyLight 550
NBP2-22164R
DyLight 594
NBP2-22164DL594
DyLight 650
NBP2-22164C
DyLight 680
NBP2-22164FR
DyLight 755
NBP2-22164IR
FITC
NBP2-22164F
HRP
NBP2-22164H
Janelia Fluor 549
NBP2-22164JF549
Janelia Fluor 646
NBP2-22164JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80954
100% Guarantee on every product

SLC6A3/DAT1 Antibody (mAb16) Summary

Immunogen
Synthetic peptide corresponding to the N-terminus of rat DAT1. [UniProt# P23977]
Epitope
N-terminus
Localization
Membrane; Multi-pass membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLC6A3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
  • Western Blot 1:1000
Application Notes
This DAT1 (mAb16) antibody is useful for Western blot, ICC/IF, Immunohistochemistry on paraffin-embedded sections and ELISA. In WB a band can been seen at ~70-85 kDa. Use in Immunoprecipitation reported in scientific literature (PMID: 20643191).
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP2-22164 in the following applications:
Publications
Read Publications using
NBP2-22164 in the following applications:

  • IHC-P
    1 publication
  • IP
    1 publication
  • WB
    11 publications

Reactivity Notes

Little to no human reactivity has been observed.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for SLC6A3/DAT1 Antibody (mAb16)

  • DA transporter
  • DAT1
  • DATDAT1Solute carrier family 6 member 3
  • SLC6A3
  • sodium-dependent dopamine transporter
  • solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
  • VNTR

Background

DAT1, also known as SLC6A3 and Sodium-dependent dopamine transporter, is a sodium and chloride-dependent dopamine transporter belonging to the solute carrier family 6. Dopamine transporters are distributed widely across the brain in regions with high dopaminergic activity, such as the striatum and substantia nigra. These transporters effectively provide fast acting clearance of dopamine, adrenalin and noradrenalin from the synaptic cleft, thus terminating the neurotransmitter signal. DATs have been found to interact with PRKCABP, TGFB1I1, SLC18A2 and the N-terminus of SYNGR3. DATs are also the target proteins for psychomotor stimulants such as amphetamines and cocaine. Research has implicated DATs as useful markers in detecting depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Frozen: Tyrosine Hydroxylase Antibody [NB300-109] - 10x Magnification cryo-sections mouse: primary antibody: anti-rb TH: 1:500; o.n. secondary antibody: 1:500 dk-anti-rb DyLight488. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the mushroom body. All panels show a right brain hemisphere (dorsal to the top, medial to the right), except panels M-O (posterior to the top). Tyrosine hydroxylase (TH) immunoreactivity is shown in magenta (A, D, G, J, M) and synapsin immunoreactivity in green (B, E, H, K, N). Corresponding merged images in C, F, I, L, O. Scale bars = 100 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY licence.
Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the central complex and the lateral accessory lobe. A-D: Frontal sections (immunoperoxidase preparations, dorsal to the top). E: Horizontal section (immunofluorescent preparation, posterior to the top). Scale bars = 100 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody [NBP3-12251] - Rat brain. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnfication.
Immunohistochemistry-Paraffin: SLC6A2/NET/Noradrenaline transporter Antibody [NBP3-12251] - Rat brain. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 5X magnfication.
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Western Blot: COMT Antibody [NB100-1780] - Staining of Human Liver (A) with antibody at 2 ug/mL and Ovary (B) lysate (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.
Western Blot: COMT Antibody [NB100-1780] - Staining of MCF7 (A) with antibody at 0.5 ug/mL, U251 cell lysate 1 (B) and U251 cell lysate 2 (C) (35 ug protein in RIPA buffer) with antibody at 1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: COMT Antibody [NB100-1780] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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(OTI9E11)
NBP1-47977
Flow Cytometry: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VMAT2 antibody, and then analyzed by flow cytometry.
Western Blot: VMAT2 Antibody (9E11) [NBP1-47977] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VMAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VMAT2.
Immunohistochemistry-Paraffin: VMAT2 Antibody (9E11) [NBP1-47977] - Staining of paraffin-embedded Human pancreas tissue using anti-VMAT2 mouse monoclonal antibody.
(1 Review)
(1 Publication)
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ELISA: Dopamine D2R/DRD2 Antibody [NBP2-88996] - HEK293 cells expressing dopamine receptor (D2) were treated with indicated concentrations of agonist (Quinpirol) and antagonist (Siperone) and probed with Dopamine Receptor (D2) pAb (1:1000 of a stock solution of 1ug/uL) by cellular ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean -+SEM (n=3).
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Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.
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(1 Review)
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Publications for SLC6A3/DAT1 Antibody (NBP2-22164)(12)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: IHC-P, IP, WB.


Filter By Application
IHC-P
(1)
IP
(1)
WB
(11)
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Filter By Species
Mouse
(2)
Rat
(11)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP2-22164 Applications Species
Carballo-Carbajal I, Laguna A, Romero-Gimenez J et al. Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson's disease pathogenesis Nat Commun Mar 7 2019 [PMID: 30846695] (IHC-P, Rat) IHC-P Rat
Moritz AE, Foster JD, Gorentla BK et al. Phosphorylation of dopamine transporter serine 7 modulates cocaine analog binding. J Biol Chem 2013 Jan 4 [PMID: 23161550] (WB, Rat) WB Rat
Foster JD, Yang JW, Moritz AE et al. Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux. J Biol Chem 2012 Aug 24 [PMID: 22722938] (WB, Rat, Mouse) WB Rat, Mouse
Foster JD, Vaughan RA. Palmitoylation controls dopamine transporter kinetics, degradation, and protein kinase C-dependent regulation. J Biol Chem 2011 Feb 18 [PMID: 21118819] (WB, Rat) WB Rat
Cervinski MA, Foster JD, Vaughan RA. Syntaxin 1A regulates dopamine transporter activity, phosphorylation and surface expression. Neuroscience 2010 Oct 13 [PMID: 20643191] (WB, IP, Rat) WB, IP Rat
Perry ML, Leinninger GM, Chen R et al. Leptin promotes dopamine transporter and tyrosine hydroxylase activity in the nucleus accumbens of Sprague-Dawley rats. J Neurochem 2010 Aug [PMID: 20412389] (WB, Rat) WB Rat
Furman CA, Chen R, Guptaroy B et al. Dopamine and amphetamine rapidly increase dopamine transporter trafficking to the surface: live-cell imaging using total internal reflection fluorescence microscopy. J Neurosci 2009 Mar 11 [PMID: 19279270] (WB, Rat) WB Rat
Gorentla BK, Moritz AE, Foster JD, Vaughan RA. Proline-directed phosphorylation of the dopamine transporter N-terminal domain. Biochemistry 2009 Feb 10 [PMID: 19146407] (WB, Rat) WB Rat
Chen R, Zhang M, Park S, Gnegy ME. C57BL/6J mice show greater amphetamine-induced locomotor activation and dopamine efflux in the striatum than 129S2/SvHsd mice. Pharmacol Biochem Behav 2007 May [PMID: 17524461] (WB, Mouse) WB Mouse
Johnson LA, Furman CA, Zhang M et al. Rapid delivery of the dopamine transporter to the plasmalemmal membrane upon amphetamine stimulation. Neuropharmacology 2005 Nov [PMID: 16212991] (WB, Rat) WB Rat
Show All 12 Publications.

Reviews for SLC6A3/DAT1 Antibody (NBP2-22164) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NBP2-22164:
Filter by Applications
WB
(3)
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Mouse
(3)
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Images Ratings Applications Species Date Details
Western Blot SLC6A3/DAT1 NBP2-22164
Enlarge 		5
reviewed by:
Stephan Quintin
 WB Mouse 03/21/2017
View

Summary

ApplicationWestern Blot
Sample Testedpurified monoclonall antibody
SpeciesMouse
Lot253354
  5
reviewed by:
Stephan Quintin
 WB Mouse 03/02/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse cerebellum,SLC6A3/DAT1 Antibody
SpeciesMouse
LotSLC6A3
Western Blot SLC6A3/DAT1 NBP2-22164
Enlarge 		4
reviewed by:
Verified Customer
 WB Mouse 04/08/2016
View

Summary

ApplicationWestern Blot
Sample Testedmouse brain
SpeciesMouse
LotA-1

Blocking

Blocking DetailsOdyssey (PBS) blocking buffer (Licor)

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG-IRDye 800CW
Secondary Manufacturer Cat#926-32210
Secondary Concentration1:15,000

Details

Detection NotesLicor Odyssey CLx

Product General Protocols

View specific protocols for SLC6A3/DAT1 Antibody (NBP2-22164): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC6A3/DAT1 Antibody (NBP2-22164). (Showing 1 - 1 of 1 FAQs).

  1. Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
    • Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Western Blot SLC6A3/DAT1 NBP2-22164
Enlarge
 Stephan Quintin
03/21/2017
Application: WB
Species: Mouse
read this review
 
 Stephan Quintin
03/02/2017
Application: WB
Species: Mouse
read this review
Western Blot SLC6A3/DAT1 NBP2-22164
Enlarge
 Verified Customer
04/08/2016
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol SLC6A3
Entrez
Uniprot