SLC6A3/DAT1 Antibody (mAb16)

Images

 
Western Blot: DAT1 Antibody (mAb16) [NBP2-22164] - Analysis of DAT1 expression in rat striatal membrane tissue lysate.
Immunocytochemistry/ Immunofluorescence: DAT1 Antibody (mAb16) [NBP2-22164] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with ...read more
Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Mu, Rt, Hu(-)Species Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
mAb16
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SLC6A3/DAT1 Antibody (mAb16) Summary

Immunogen
Synthetic peptide corresponding to the N-terminus of rat DAT1. [UniProt# P23977]
Epitope
N-terminus
Localization
Membrane; Multi-pass membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLC6A3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
Application Notes
This DAT1 (mAb16) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. In WB a band can been seen at ~70-85 kDa. Use in Immunoprecipitation reported in scientific literature (PMID: 20643191).
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP2-22164 in the following applications:

Publications
Read Publications using
NBP2-22164 in the following applications:

  • IP
    1 publication
  • WB
    11 publications

Reactivity Notes

Mouse and rat. Little to no human reactivity has been observed.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for SLC6A3/DAT1 Antibody (mAb16)

  • DA transporter
  • DAT1
  • DATDAT1Solute carrier family 6 member 3
  • SLC6A3
  • sodium-dependent dopamine transporter
  • solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
  • VNTR

Background

DAT1, also known as SLC6A3 and Sodium-dependent dopamine transporter, is a sodium and chloride-dependent dopamine transporter belonging to the solute carrier family 6. Dopamine transporters are distributed widely across the brain in regions with high dopaminergic activity, such as the striatum and substantia nigra. These transporters effectively provide fast acting clearance of dopamine, adrenalin and noradrenalin from the synaptic cleft, thus terminating the neurotransmitter signal. DATs have been found to interact with PRKCABP, TGFB1I1, SLC18A2 and the N-terminus of SYNGR3. DATs are also the target proteins for psychomotor stimulants such as amphetamines and cocaine. Research has implicated DATs as useful markers in detecting depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for SLC6A3/DAT1 Antibody (NBP2-22164)(11)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(11)
All Applications
Filter By Species
Mouse
(2)
Rat
(10)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-22164 Applications Species
Moritz AE, Foster JD, Gorentla BK et al. Phosphorylation of dopamine transporter serine 7 modulates cocaine analog binding. J Biol Chem 2013 Jan 4 [PMID: 23161550] (WB, Rat) WB Rat
Foster JD, Yang JW, Moritz AE et al. Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux. J Biol Chem 2012 Aug 24 [PMID: 22722938] (WB, Rat, Mouse) WB Rat, Mouse
Foster JD, Vaughan RA. Palmitoylation controls dopamine transporter kinetics, degradation, and protein kinase C-dependent regulation. J Biol Chem 2011 Feb 18 [PMID: 21118819] (WB, Rat) WB Rat
Cervinski MA, Foster JD, Vaughan RA. Syntaxin 1A regulates dopamine transporter activity, phosphorylation and surface expression. Neuroscience 2010 Oct 13 [PMID: 20643191] (WB, IP, Rat) WB, IP Rat
Perry ML, Leinninger GM, Chen R et al. Leptin promotes dopamine transporter and tyrosine hydroxylase activity in the nucleus accumbens of Sprague-Dawley rats. J Neurochem 2010 Aug [PMID: 20412389] (WB, Rat) WB Rat
Furman CA, Chen R, Guptaroy B et al. Dopamine and amphetamine rapidly increase dopamine transporter trafficking to the surface: live-cell imaging using total internal reflection fluorescence microscopy. J Neurosci 2009 Mar 11 [PMID: 19279270] (WB, Rat) WB Rat
Gorentla BK, Moritz AE, Foster JD, Vaughan RA. Proline-directed phosphorylation of the dopamine transporter N-terminal domain. Biochemistry 2009 Feb 10 [PMID: 19146407] (WB, Rat) WB Rat
Chen R, Zhang M, Park S, Gnegy ME. C57BL/6J mice show greater amphetamine-induced locomotor activation and dopamine efflux in the striatum than 129S2/SvHsd mice. Pharmacol Biochem Behav 2007 May [PMID: 17524461] (WB, Mouse) WB Mouse
Johnson LA, Furman CA, Zhang M et al. Rapid delivery of the dopamine transporter to the plasmalemmal membrane upon amphetamine stimulation. Neuropharmacology 2005 Nov [PMID: 16212991] (WB, Rat) WB Rat
Johnson LA, Guptaroy B, Lund D et al. Regulation of amphetamine-stimulated dopamine efflux by protein kinase C beta. J Biol Chem 2005 Mar 25 [PMID: 15647254] (WB, Rat) WB Rat
Show All 11 Publications.

Reviews for SLC6A3/DAT1 Antibody (NBP2-22164) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.
We have 3 reviews tested in 1 application: WB.

Reviews using NBP2-22164:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot SLC6A3/DAT1 NBP2-22164
Enlarge
reviewed by:
Stephan Quintin
WB Mouse 03/21/2017
View

Summary

ApplicationWestern Blot
Sample Testedpurified monoclonall antibody
SpeciesMouse
Lot253354
 
reviewed by:
Stephan Quintin
WB Mouse 03/02/2017
View

Summary

ApplicationWestern Blot
Sample TestedSLC6A3/DAT1 Antibody,mouse cerebellum
SpeciesMouse
LotSLC6A3
Western Blot SLC6A3/DAT1 NBP2-22164
Enlarge
reviewed by:
Anonymous
WB Mouse 04/08/2016
View

Summary

ApplicationWestern Blot
Sample Testedmouse brain
SpeciesMouse
LotA-1

Blocking

Blocking DetailsOdyssey (PBS) blocking buffer (Licor)

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG-IRDye 800CW
Secondary Manufacturer Cat#926-32210
Secondary Concentration1:15,000

Details

Detection NotesLicor Odyssey CLx

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC6A3/DAT1 Antibody (NBP2-22164). (Showing 1 - 1 of 1 FAQs).

  1. Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
    • Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-22164AF405
Alexa Fluor 488 Labeled NBP2-22164AF488
Alexa Fluor 647 Labeled NBP2-22164AF647
Alexa Fluor 700 Labeled NBP2-22164AF700
DyLight 350 Labeled NBP2-22164UV
DyLight 405 Labeled NBP2-22164V
DyLight 488 Labeled NBP2-22164G
DyLight 680 Labeled NBP2-22164FR
DyLight 755 Labeled NBP2-22164IR

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Stephan Quintin
03/21/2017
Application: WB
Species: Mouse

 
Stephan Quintin
03/02/2017
Application: WB
Species: Mouse

Anonymous
04/08/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SLC6A3
Entrez
Uniprot