SLC6A3/DAT1 Antibody (mAb16)

Western Blot: DAT1 Antibody (mAb16) [NBP2-22164] - Analysis of DAT1 expression in rat striatal membrane tissue lysate.

Immunocytochemistry/Immunofluorescence: DAT1 Antibody (mAb16) [NBP2-22164] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with Phalloidin-AlexaFluor 568 (Red) and DAPI (Blue), respectively.

Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.

• Reactivity: Mu, Rt, Hu(-)
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
• Clone: mAb16
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SLC6A3/DAT1 Antibody (mAb16) Summary

• Immunogen: Synthetic peptide corresponding to the N-terminus of rat DAT1. [UniProt# P23977]
• Epitope: N-terminus
• Localization: Membrane; Multi-pass membrane protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC6A3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation
• Application Notes: This DAT1 (mAb16) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. In WB a band can been seen at ~70-85 kDa. Use in Immunoprecipitation reported in scientific literature (PMID: 20643191).

Reactivity Notes

Mouse and rat. Little to no human reactivity has been observed.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for SLC6A3/DAT1 Antibody (mAb16)

• DA transporter
• DAT1
• DATDAT1Solute carrier family 6 member 3
• SLC6A3
• sodium-dependent dopamine transporter
• solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• VNTR

Background

DAT1, also known as SLC6A3 and Sodium-dependent dopamine transporter, is a sodium and chloride-dependent dopamine transporter belonging to the solute carrier family 6. Dopamine transporters are distributed widely across the brain in regions with high dopaminergic activity, such as the striatum and substantia nigra. These transporters effectively provide fast acting clearance of dopamine, adrenalin and noradrenalin from the synaptic cleft, thus terminating the neurotransmitter signal. DATs have been found to interact with PRKCABP, TGFB1I1, SLC18A2 and the N-terminus of SYNGR3. DATs are also the target proteins for psychomotor stimulants such as amphetamines and cocaine. Research has implicated DATs as useful markers in detecting depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC6A3/DAT1 Antibody (NBP2-22164)(11)

Publications using NBP2-22164

• Moritz AE, Foster JD, Gorentla BK et al. Phosphorylation of dopamine transporter serine 7 modulates cocaine analog binding. J Biol Chem 2013 Jan 4 [PMID: 23161550] (WB, Rat)
• Foster JD, Yang JW, Moritz AE et al. Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux. J Biol Chem 2012 Aug 24 [PMID: 22722938] (WB, Rat, Mouse)
• Foster JD, Vaughan RA. Palmitoylation controls dopamine transporter kinetics, degradation, and protein kinase C-dependent regulation. J Biol Chem 2011 Feb 18 [PMID: 21118819] (WB, Rat)
• Cervinski MA, Foster JD, Vaughan RA. Syntaxin 1A regulates dopamine transporter activity, phosphorylation and surface expression. Neuroscience 2010 Oct 13 [PMID: 20643191] (WB, IP, Rat)
• Perry ML, Leinninger GM, Chen R et al. Leptin promotes dopamine transporter and tyrosine hydroxylase activity in the nucleus accumbens of Sprague-Dawley rats. J Neurochem 2010 Aug [PMID: 20412389] (WB, Rat)
• Furman CA, Chen R, Guptaroy B et al. Dopamine and amphetamine rapidly increase dopamine transporter trafficking to the surface: live-cell imaging using total internal reflection fluorescence microscopy. J Neurosci 2009 Mar 11 [PMID: 19279270] (WB, Rat)
• Gorentla BK, Moritz AE, Foster JD, Vaughan RA. Proline-directed phosphorylation of the dopamine transporter N-terminal domain. Biochemistry 2009 Feb 10 [PMID: 19146407] (WB, Rat)
• Chen R, Zhang M, Park S, Gnegy ME. C57BL/6J mice show greater amphetamine-induced locomotor activation and dopamine efflux in the striatum than 129S2/SvHsd mice. Pharmacol Biochem Behav 2007 May [PMID: 17524461] (WB, Mouse)
• Johnson LA, Furman CA, Zhang M et al. Rapid delivery of the dopamine transporter to the plasmalemmal membrane upon amphetamine stimulation. Neuropharmacology 2005 Nov [PMID: 16212991] (WB, Rat)
• Johnson LA, Guptaroy B, Lund D et al. Regulation of amphetamine-stimulated dopamine efflux by protein kinase C beta. J Biol Chem 2005 Mar 25 [PMID: 15647254] (WB, Rat)
• Gaffaney JD, Vaughan RA. Uptake inhibitors but not substrates induce protease resistance in extracellular loop two of the dopamine transporter. Mol Pharmacol 2004 Mar [PMID: 14978248] (WB, Rat)

Reviews for SLC6A3/DAT1 Antibody (NBP2-22164) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Stephan Quintin WB Mouse 03/21/2017

Summary

• Application: Western Blot
• Sample Tested: purified monoclonall antibody
• Species: Mouse
• Lot: 253354

reviewed by: Stephan Quintin WB Mouse 03/02/2017

Summary

• Application: Western Blot
• Sample Tested: SLC6A3/DAT1 Antibody,mouse cerebellum
• Species: Mouse
• Lot: SLC6A3

reviewed by: Anonymous WB Mouse 04/08/2016

Summary

• Application: Western Blot
• Sample Tested: mouse brain
• Species: Mouse
• Lot: A-1

Blocking

• Blocking Details: Odyssey (PBS) blocking buffer (Licor)

Primary Anitbody

• Dilution Ratio: 1:1000

Secondary Antibody

• Secondary Description: Goat anti-mouse IgG-IRDye 800CW
• Secondary Manufacturer Cat#: 926-32210
• Secondary Concentration: 1:15,000

Details

• Detection Notes: Licor Odyssey CLx

FAQs for SLC6A3/DAT1 Antibody (NBP2-22164). (Showing 1 - 1 of 1 FAQs).

1. Could you kindly provide published references for Western blot in detection of Dopamine transporter in human brain tissue with your antibody/antibodies?
   • Catalog numbers NBP2-22164, NB300-254 and NB100-65459 are well characterized DAT1 antibodies that have been referenced. If you refer to our website we have links to the PMID listing for each reference on the individual datasheets.

Bioinformatics

• Gene Symbol: SLC6A3
• Entrez:
  • 6531(Human)
  • 13162(Mouse)
  • 24898(Rat)
• Uniprot:
  • Q61327(Mouse)
  • P23977(Rat)