Glutamine Synthetase Antibody

Images

 
Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lanes R), pig (Lanes P), bovine (Lanes B), or human (Lanes Hu) retina were probed. A ...read more
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glutamine Synthetase Antibody Summary

Immunogen
Recombinant Glutamine Synthetase.
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GLUL
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunohistochemistry 1:12000
Application Notes
This Glutamine Synthetase antibody is useful for Immunohistochemistry and Western Blot.
Positive Control
Glutamine Synthetase Lysate (NBL1-11130)
Reviewed Applications
Read 1 Review rated 4
using
NB110-41404 in the following applications:

Publications
Read Publications using
NB110-41404 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Bovine, human, mouse, rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Glutamine Synthetase Antibody

  • cell proliferation-inducing protein 59
  • EC 4.1.1.15
  • EC 6.3.1.2
  • GLNSPIG59
  • Glutamate decarboxylase
  • glutamate-ammonia ligase (glutamine synthase)
  • glutamate-ammonia ligase
  • Glutamate--ammonia ligase
  • glutamine synthase
  • glutamine synthetase
  • GS
  • PIG43
  • proliferation-inducing protein 43

Background

Glutamine synthetase (GLUL) is an enzyme responsible for the de novo synthesis of glutamine (from glutamate and ammonia, wherein glutamate act as nitrogen donor/acceptor) and for production of gamma-aminobutyric acid/GABA (4-aminobutanoate). GLUL is expressed in a wide variety of human cells, such as astrocytes (marker rotein), skeletal cells, adipocytes, hepatocytes and sperms. GLUL activity in vertebrate brain is generally high as it protects this ammonia-sensitive organ from harmful effects of ammonia and is involves in recycling of neurotransmitter glutamate also. GLUL is essential in maintaining cellular homeostasis and besides its involvement in ammonia detoxification, GLUL participates in maintaining amino acid/energy balance, cellular redox control, nucleotide biosynthesis and neurotransmitters regulation. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) which is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC

Publications for Glutamine Synthetase Antibody (NB110-41404)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Filter By Species
Mouse
(2)
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Review for Glutamine Synthetase Antibody (NB110-41404) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-41404:
Filter by Applications
IF
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Glutamine Synthetase NB110-41404
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reviewed by:
Anonymous
IF Mouse 05/11/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse dorsal root ganglia
SpeciesMouse
LotD-1

Blocking

Blocking DetailsPBS BSA 2% Triton 0.4%, RT

Primary Anitbody

Dilution Ratio1/5000, overnight, 4C, PBS BSA 1% Triton 0.2%

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 546
Secondary Manufacturer Cat#A10040
Secondary Concentration1/500, RT, 1h, PBS BSA 1% Triton 0.2%

Details

Detection NotesOlympus BX63
Fixation DetailsPFA 4%
Wash DescriptionPBS

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Glutamine Synthetase Antibody (NB110-41404). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for a primary antibody specific to the cytosolic isoform of glutamine synthetase.
    • I see no evidence that glutamine synthetase has multiple isoforms. Please see this link to UniProt for more details. NB110-41404 should work just fine for you as the only known isoform appears to be cytoplasmic.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Glutamine Synthetase Antibody (NB110-41404)

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Diseases for Glutamine Synthetase Antibody (NB110-41404)

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PTMs for Glutamine Synthetase Antibody (NB110-41404)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
05/11/2016
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol GLUL
Entrez