TLR7 Antibody (4F4)

Images

 
Western Blot: TLR7 Antibody (4F4) [NBP1-04349] - The Ramos cell lysates 80ug were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TLR7 antibody (1:1000). Proteins were visualized using a ...read more
Immunocytochemistry/ Immunofluorescence: TLR7 Antibody (4F4) [NBP1-04349] - Analysis in THP-1 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TLR7 antibody (1:100) with goat ...read more
Flow Cytometry: TLR7 Antibody (4F4) [NBP1-04349] - Analysis of TLR7 in THP-1 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
4F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TLR7 Antibody (4F4) Summary

Immunogen
Recombinant human TLR7 (451-500aa) purified from E. coli
Specificity
Anti-human TLR7 mAb, clone 4F4, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TLR7 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TLR7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
Agonist

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TLR7 Antibody (4F4)

  • TLR7
  • toll-like receptor 7

Background

TLR7 is one of toll-like receptors which are type I transmembrane proteins involved in innate immunity by recognizing microbial conserved structures. These proteins are characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain, and act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Human TLR7(hTLR7) is predominantly expressed in lung, placenta and spleen and hTLR7,8 genes are located on the sex chromosome X. hTLR7 may play an important role in detecting and combating viral infections.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Bioinformatics

Gene Symbol TLR7
Entrez
Uniprot