TLR5 Antibody (85B152.5) - Azide and BSA Free

Western Blot: TLR5 Antibody (85B152.5) - Azide Free [NBP2-33253] - Normal human bronchial epithelial total cell lysate. Image from verified customer review. Image from the standard format of this antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) - Azide Free [NBP2-33253] - Intracellular flow analysis of TLR5 in 10^6 human lymphocytes using 0.5 ug of NB200-571. The shaded histogram represents cells without antibody, green represents isotype control (BD), and red represents TLR5 antibody. Image from the standard format of this antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) - Azide Free [NBP2-33253] - Analysis using the FITC conjugate of NBP1-97728. Staining of TLR5 in 10^6 cells using 0.5 ugs of FITC-conjugated antibody: A) Intracellular flow testing of human Ramos cells and B) cell surface flow testing of mouse RAW cells. The shaded (or black) histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) - Azide Free [NBP2-33253] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) - Azide Free [NBP2-33253] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) - Azide Free [NBP2-33253] - Intracellular flow analysis of TLR5 in Ramos cells using 1 ug of TLR5 antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control antibody; red represents TLR5 antibody. Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Ca
• Applications: WB, Flow
• Clone: 85B152.5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TLR5 Antibody (85B152.5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TLR5 Antibody (85B152.5) - Azide and BSA Free (NBP1-97728) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5. It will cross-react with mouse TLR5 (NP_003259).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) reported in scientific literature (Wong et al (2007))
  • Flow Cytometry
  • Western Blot 1-3 ug/ml
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Agonist:
  • Flagellin, TLR5 Ligand NBP2-25289

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for TLR5 Antibody (85B152.5) - Azide and BSA Free

• MGC126430
• MGC126431
• SLEB1FLJ10052
• TIL-3
• TIL3Toll/interleukin-1 receptor-like protein 3
• TLR5
• toll-like receptor 5

Background

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLR5