TLR5 Antibody (85B152.5) [PE]


Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - Intracellular flow analysis of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, more
Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - Cell surface analysis of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green more

Product Details

Reactivity Hu, Mu, CaSpecies Glossary
Applications Flow, Flow-CS, Flow-IC

TLR5 Antibody (85B152.5) [PE] Summary

This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5. It will cross-react with mouse TLR5 (NP_003259).
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1ul/1 million cells
  • Flow (Cell Surface)
  • Flow (Intracellular)
Application Notes
Reported use in Flow Cytometry (cell surface): see Wong et al, 2007 for details. Use in Flow cell surface and Flow intracellular reported in scientific literature (PMID 24779433) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP2-24959 in the following applications:

Packaging, Storage & Formulations

Store at 4C in the dark.
0.05% Sodium Azide
Protein G purified

Alternate Names for TLR5 Antibody (85B152.5) [PE]

  • MGC126430
  • MGC126431
  • SLEB1FLJ10052
  • TIL-3
  • TIL3Toll/interleukin-1 receptor-like protein 3
  • TLR5
  • toll-like receptor 5


The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
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Applications: WB, Flow, ICC/IF, IP, B/N, Flow-CS
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
Species: Hu, Mu, Rt, Ca, Eq, Mk, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, In vitro, CyTOF-ready, Flow-IC
Species: Hu
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC, IF
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu, Rb
Applications: WB, Flow, IHC-P
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA
Species: Hu
Applications: Flow, IHC, IHC-P, Flow-CS, Flow-IC
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Flow-IC, IF
Species: Hu
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
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Publications for TLR5 Antibody (NBP2-24959)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 3 applications: FLOW, Flow-CS, Flow-IC.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP2-24959 Applications Species
Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes Veterinary Parasitology Feb 15 2016 [PMID: 26995719] (FLOW, Canine) FLOW Canine
Mishra A, Brown AL, Yao X et al. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase Nat Commun. 2015 Feb 19 [PMID: 25692509] (FLOW, Mouse) FLOW Mouse
Bens M, Vimont S, Ben Mkaddem S et al. Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli. Cell. Microbiol. 2014 Apr 29 [PMID: 24779433] (Flow-CS, Flow-IC, Mouse)

Primary cultures of renal medullary collecting duct cells (MpkIMCD) from mouse kidney: Flow (cell surface) & Flow (intracellular), Fig 1E. Flow (cell surface) was done on non-permeabilized cells (Fig 1E, left panel) and Flow (intracellular) was done on pe
Flow-CS, Flow-IC Mouse
Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013 Apr 8 [PMID: 23593278]

Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
Feng T, Cong Y, Alexander K, Elson CO. Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells. PLoS One. 2012 [PMID: 22545147]

Antibodies cited: TLR5 (IMG-663A), Flow-intracellular (DC cells derived from C57BL/6 mice): Fig 1c, d: lamina propria (LP) from the intestine and splenic DCs. The cells were doubled stained with a CD11b mAb and the IMG-663A TLR5 mAb. The LP DCs but not th
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009 Sep [PMID: 20196781] (Human)

flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.
Hafez M, Hayes K, Goldrick M et al. The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells. Infect Immun. 2010 May [PMID: 20145095]

IMGENEX products cited: 1. TLR4 (IMG- 5031A) Flow (Cell surface), Fig 3, Caco-2 cells. 2. TLR5 (IMG-663A) Flow (Cell surface), Fig 2, Fig 3, Caco-2 cells.
Rydberg C, Mansson A, Uddman R et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology. 2009 Sep [PMID: 19740321]

TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315A), TLR3/CD283 (IMG-315D), TLR5 PE (IMG-663D), TLR5 (IMG-663). 1. IHC(paraffin): Human head and neck squamous (HNSCC) cell carcinomas showing keratinized cell carcinoma from the larynx stained with TLR2 (IMG-416A) and TLR5 antibodies (IMG-663A), Fig 1A, 1B, & 1C. 2. Flow (intracellular): TLR2 PE (IMG-416D), TLR3 PE (IMG-315D), and TLR5 PE (IMG-663D) antibodies were used in human bronchial epithelial (NL-20) and human pharyngeal squamous (Detroit-562) carcinoma cell line, Fig 2C.
Wu H, Wang H, Xiong W et al. Expression patterns and functions of toll-like receptors in mouse sertoli cells. Endocrinology. 2008 Sep [PMID: 18499758]

Antibodies cited: 1. TLR2 (IMG-410A): WB (sertoli cell lysates), Fig. 1D. 2. TLR5 (IMG-444A): WB (sertoli cell lysates), Fig. 1D. 3. TLR5 (IMG-664C): Flow (cell surface), mouse sertoli cells and macrophages, Figs. 1B,C,E. 4. TLR6 (IMG-527): WB (sertoli ce
Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008 Jul 15 [PMID: 18439687]
Show All 15 Publications.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-97728APC
Biotin Labeled NBP1-97728B
DyLight 350 Labeled NBP1-97728UV
DyLight 405 Labeled NBP1-97728V
DyLight 488 Labeled NBP1-97728G
DyLight 550 Labeled NBP1-97728R
DyLight 650 Labeled NBP1-97728C
DyLight 680 Labeled NBP1-97728FR
DyLight 755 Labeled NBP1-97728IR
HRP Labeled NBP1-97728H
PerCP Labeled NBP1-97728PCP

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Blogs on TLR5.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

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Gene Symbol TLR5