TLR5 Antibody (85B152.5) [PE]

Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - A surface stain was performed on A431 cells with TLR5 [85B152.5] Antibody NBP2-24959 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - Cell surface analysis of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - Intracellular flow analysis of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

• Reactivity: Hu, Mu, Ca
• Applications: Flow
• Clone: 85B152.5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

TLR5 Antibody (85B152.5) [PE] Summary

• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5. It will cross-react with mouse TLR5 (NP_003259).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow (Intracellular)
  • Flow Cytometry 1ul/1 million cells
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Agonist:
  • Flagellin, TLR5 Ligand NBP2-25289

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for TLR5 Antibody (85B152.5) [PE]

• MGC126430
• MGC126431
• SLEB1FLJ10052
• TIL-3
• TIL3Toll/interleukin-1 receptor-like protein 3
• TLR5
• toll-like receptor 5

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR5 Antibody (NBP2-24959)(16)

Publications using NBP2-24959

• Kumazoe M, Takamatsu K, Horie F et al. Methylated (-)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5 Sci Rep 2021-11-29 [PMID: 34845235] (FLOW, Mouse)
  
  Details:
  Citation using the PE format of this antibody.
• Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine)
  
  Details:
  Used the PE form of this antibody.
• Mishra A, Brown AL, Yao X et al. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase Nat Commun Nat Commun [PMID: 25692509] (FLOW, Mouse)
  
  Details:
  This publication used the PE conjugated form of this antibody (Cat# NBP2-24959).
• Bens M, Vimont S, Ben Mkaddem S et al. Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli Cell Microbiol 2014-04-29 [PMID: 24779433] (Flow Cytometry Control, Flow-CS, Mouse)
  
  Details:
  This citation used the PE version of this antibody.
• Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013-04-08 [PMID: 23593278]
  
  Details:
  Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
• Feng T, Cong Y, Alexander K, Elson CO. Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells. PLoS One. 2012-01-01 [PMID: 22545147]
  
  Details:
  Antibodies cited: TLR5 (IMG-663A), Flow-intracellular (DC cells derived from C57BL/6 mice): Fig 1c, d: lamina propria (LP) from the intestine and splenic DCs. The cells were doubled stained with a CD11b mAb and the IMG-663A TLR5 mAb. The LP DCs but not th
• van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009-09-01 [PMID: 20196781] (Human)
  
  Details:
  flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.
• Hafez M, Hayes K, Goldrick M et al. The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells. Infect Immun. 2010-05-01 [PMID: 20145095]
  
  Details:
  IMGENEX products cited: 1. TLR4 (IMG- 5031A) Flow (Cell surface), Fig 3, Caco-2 cells. 2. TLR5 (IMG-663A) Flow (Cell surface), Fig 2, Fig 3, Caco-2 cells.
• Rydberg C, Mansson A, Uddman R et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology. 2009-09-01 [PMID: 19740321]
  
  Details:
  TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315A), TLR3/CD283 (IMG-315D), TLR5 PE (IMG-663D), TLR5 (IMG-663). 1. IHC(paraffin): Human head and neck squamous (HNSCC) cell carcinomas showing keratinized cell carcinoma from the larynx stained with TLR2 (IMG-416A) and TLR5 antibodies (IMG-663A), Fig 1A, 1B, & 1C. 2. Flow (intracellular): TLR2 PE (IMG-416D), TLR3 PE (IMG-315D), and TLR5 PE (IMG-663D) antibodies were used in human bronchial epithelial (NL-20) and human pharyngeal squamous (Detroit-562) carcinoma cell line, Fig 2C.
• Wu H, Wang H, Xiong W et al. Expression patterns and functions of toll-like receptors in mouse sertoli cells. Endocrinology. 2008-09-01 [PMID: 18499758]
  
  Details:
  Antibodies cited: 1. TLR2 (IMG-410A): WB (sertoli cell lysates), Fig. 1D. 2. TLR5 (IMG-444A): WB (sertoli cell lysates), Fig. 1D. 3. TLR5 (IMG-664C): Flow (cell surface), mouse sertoli cells and macrophages, Figs. 1B,C,E. 4. TLR6 (IMG-527): WB (sertoli ce
• Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687]
  
  Details:
  Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
• Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008-03-01 [PMID: 18292566]
  
  Details:
  Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
• Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440]
  
  Details:
  Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
• LeBouder E, Rey-Nores JE, Raby AC et al. Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk. J Immunol. 2006-03-15 [PMID: 16517743]
  
  Details:
  1. TLR3 (IMG-315) [Flow (Cell Surface), Fig.3 (dendritic cells)]. 2. TLR5 (IMG-663) [Flow (Cell Surface), Fig.2 (dendritic cells)].
• Mansson A, Adner M, Cardell LO. Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis. Respir Res. 2006-02-27 [PMID: 16504163]
  
  Details:
  Antibodies cited (human tonsils separated into cell subtypes): 1. TLR3 [IMG-315D (Flow-Intracellular), Figs 5 and 6]. 2. TLR5 [IMG-663A (Flow-Intracellular), Fig 6]. 3. TLR9 [IMG-305C (Flow-Intracellular), Fig 4.].
• Crellin NK Garcia RV Hadisfar O et al. Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells. J Immunol. [PMID: 16339542]
  
  Details:
  Citation using the Azide and Endotoxin Free form of this antibody.

Bioinformatics

• Gene Symbol: TLR5