TLR5 Antibody (85B152.5)

Western Blot: TLR5 Antibody (85B152.5) [NBP1-97728] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.

Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the FITC conjugate of NBP1-97728. Staining of TLR5 in 10^6 cells using 0.5 ugs of FITC-conjugated antibody: A) Intracellular flow testing of human Ramos cells and B) cell surface flow testing of mouse RAW cells. The shaded (or black) histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow analysis of TLR5 in Ramos cells using 1 ug of TLR5 antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control antibody; red represents TLR5 antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow Analysis of TLR5 in 10^6 human lymphocytes using 0.5 ug of NB200-571. The shaded histogram represents cells without antibody, green represents isotype control (BD), and red represents TLR5 antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 a

• Reactivity: Hu, Mu, Ca
• Applications: WB, Flow, Flow-CS, Flow-IC, IHC-P
• Clone: 85B152.5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TLR5 Antibody (85B152.5) Summary

• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5. It will cross-react with mouse TLR5 (NP_003259).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml
  • Flow Cytometry 1-3 ug/10^6 cells in 100 ul
  • Immunohistochemistry-Paraffin
  • Flow (Cell Surface)
  • Flow (Intracellular)
• Application Notes: Reported use in Flow Cytometry (cell surface): see Wong et al, 2007 for details. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 17570691). Use in Flow-cell surface and Flow-intracellular reported in scientific literature (PMID 24779433) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Agonist:
  • Flagellin, TLR5 Ligand

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TLR5 Antibody (85B152.5)

• MGC126430
• MGC126431
• SLEB1FLJ10052
• TIL-3
• TIL3Toll/interleukin-1 receptor-like protein 3
• TLR5
• toll-like receptor 5

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR5 Antibody (NBP1-97728)(30)

Publications using NBP1-97728

• Kato K, Lillehoj EP, Kim KC. Pseudomonas aeruginosa stimulates tyrosine phosphorylation of and TLR5 association with the MUC1 cytoplasmic tail through EGFR activation. Inflamm. Res. Mar 1 2016 [PMID: 26645913] (WB, Human)
• Bens M, Vimont S, Ben Mkaddem S et al. Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli Cell Microbiol 2014 Apr 29 [PMID: 24779433] (Flow-CS, Flow-IC, Mouse)
  
  Details:
  This citation used the PE version of this antibody.
• Sawitzki B, Brunstein C, Meisel C et al. Prevention of Graft-versus-Host Disease by Adoptive T Regulatory Therapy Is Associated with Active Repression of Peripheral Blood Toll-Like Receptor 5 mRNA Expression. Biol Blood Marrow Transplant [PMID: 24184334] (FLOW, Human)
  
  Details:
  Used the FITC form of this antibody.
• Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013 Apr 8 [PMID: 23593278] (WB, Flow-IC)
  
  Details:
  Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
• Bens M, Vimont S, Ben Mkaddem S et al. Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli Cell Microbiol (Flow-CS, Flow-IC, Mouse)
  
  Details:
  This publication used the PE conjugated form of this antibody (Cat# NBP2-24959).
• He B, Xu W, Santini PA et al. Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL Immunity (IHC-P)
  
  Details:
  This publication used the FITC conjugated form of this antibody (Cat# NB200-571).
• Riccioli A, Starace D, Galli R et al. Sertoli cells initiate testicular innate immune responses through TLR activation J Immunol (Flow-CS)
  
  Details:
  This publication used the FITC conjugated form of this antibody (Cat# NB200-571).
• Sawitzki B, Brunstein C, Meisel C et al. Prevention of Graft-versus-Host Disease by Adoptive T Regulatory Therapy Is Associated with Active Repression of Peripheral Blood Toll-Like Receptor 5 mRNA Expression. Biol Blood Marrow Transplant (FLOW, Human)
  
  Details:
  This publication used the FITC conjugated form of this antibody (Cat# NB200-571).
• Mastorci K, Muraro E, Pasini E et al. Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma. PLoS ONE. 2016 April 29 [PMID: 27123851] (Flow, Human)
  
  Details:
  This citation used the FITC version of this antibody.
• Mishra A, Brown AL, Yao X et al. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase Nat Commun Nat Commun [PMID: 25692509] (FLOW, Mouse)
  
  Details:
  This publication used the PE conjugated form of this antibody (Cat# NBP2-24959).
• Li Z, Younger K, Gartenhaus R et al. Inhibition of IRAK1/4 sensitizes T cell acute lymphoblastic leukemia to chemotherapies J. Clin. Invest. 2015 Mar 02 [PMID: 25642772] (WB, Human)
• Feng T, Cong Y, Alexander K, Elson CO. Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells. PLoS One. 2012 [PMID: 22545147] (Flow-IC, Mouse)
  
  Details:
  Antibodies cited: TLR5 (IMG-663A), Flow-intracellular (DC cells derived from C57BL/6 mice): Fig 1c, d: lamina propria (LP) from the intestine and splenic DCs. The cells were doubled stained with a CD11b mAb and the IMG-663A TLR5 mAb. The LP DCs but not th
• van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009 Sep [PMID: 20196781] (Flow-CS, Human)
  
  Details:
  The following antibodies were used in flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.
• Francois S et al. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad J Immunol
  
  Details:
  This publication used the FITC conjugated form of this antibody (Cat# NB200-571).
• Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine)
  
  Details:
  Used the PE form of this antibody.
• Hafez M, Hayes K, Goldrick M et al. The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells. Infect Immun. 2010 May [PMID: 20145095] (Flow-CS)
  
  Details:
  IMGENEX products cited: 1. TLR4 (IMG- 5031A) Flow (Cell surface), Fig 3, Caco-2 cells. 2. TLR5 (IMG-663A) Flow (Cell surface), Fig 2, Fig 3, Caco-2 cells.
• Rydberg C, Mansson A, Uddman R et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology. 2009 Sep [PMID: 19740321]
  
  Details:
  TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315A), TLR3/CD283 (IMG-315D), TLR5 PE (IMG-663D), TLR5 (IMG-663). 1. IHC(paraffin): Human head and neck squamous (HNSCC) cell carcinomas showing keratinized cell carcinoma from the larynx stained with TLR2 (IMG-416A) and TLR5 antibodies (IMG-663A), Fig 1A, 1B, & 1C. 2. Flow (intracellular): TLR2 PE (IMG-416D), TLR3 PE (IMG-315D), and TLR5 PE (IMG-663D) antibodies were used in human bronchial epithelial (NL-20) and human pharyngeal squamous (Detroit-562) carcinoma cell line, Fig 2C.
• Wu H, Wang H, Xiong W et al. Expression patterns and functions of toll-like receptors in mouse sertoli cells. Endocrinology. 2008 Sep [PMID: 18499758] (Flow-CS, WB)
  
  Details:
  Antibodies cited: 1. TLR2 (IMG-410A): WB (sertoli cell lysates), Fig. 1D. 2. TLR5 (IMG-444A): WB (sertoli cell lysates), Fig. 1D. 3. TLR5 (IMG-664C): Flow (cell surface), mouse sertoli cells and macrophages, Figs. 1B,C,E. 4. TLR6 (IMG-527): WB (sertoli ce
• Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008 Jul 15 [PMID: 18439687]
• Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008 Mar 1 [PMID: 18292566]
  
  Details:
  Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
• He B, Xu W, Santini PA et al. Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL Immunity 2007 Jun [PMID: 17570691] (IHC-P)
  
  Details:
  This citation used the FITC version of this antibody.
• Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007 Jul. [PMID: 17332440]
  
  Details:
  Antibodies cited: WB (human blood eosinophils and neutrophils from buffy coat), Fig. 1a: 1. TLR1 [IMG-5012] 2. TLR5 [IMG-664] 3. TLR6 [IMG-304A] 4. TLR7 [IMG-540] 5. TLR8 [IMG-321A] 6. TLR9 [IMG-305A] Flow (Cell Surface and Intracellular) (human blood eos
• Riccioli A, Starace D, Galli R et al. Sertoli cells initiate testicular innate immune responses through TLR activation J Immunol 2006 Nov 15 [PMID: 17082629] (Flow-CS)
  
  Details:
  This citation used the FITC version of this antibody.
• LeBouder E, Rey-Nores JE, Raby AC et al. Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk. J Immunol. 2006 Mar 15 [PMID: 16517743] (Flow)
  
  Details:
  Antibodies cited: 1 TLR3 (IMG-315[FC]); 2. TLR5 (IMG-663 [FC]).
• Mansson A, Adner M, Cardell LO. Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis. Respir Res. 2006 Feb 27 [PMID: 16504163] (Flow-IC, Human)
  
  Details:
  Human tonsils separated into cell subtypes: 1. TLR3 [IMG-315D (Flow-Intracellular), Figs 5 and 6]. 2. TLR5 [IMG-663A (Flow-Intracellular), Fig 6]. 3. TLR9 [IMG-305C (Flow-Intracellular), Fig 4.]
• Crellin NK, Garcia RV, Hadisfar O et al. Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells. J Immunol. 2005 Dec 15 [PMID: 16339542]
  
  Details:
  Antibodies cited: 1. TLR5-FITC (IMG-663C) [Flow-intracellular, Figs 3B and 3D (primary human CD4+ T cells, primary human CD14+ monocytes, primary human monocyte-derived dendritic cells)]; 2, TLR5 (IMG-664) [WB, Fig 5 (primary human CD4+ T cells)]. The spe
• Francois S et al. Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad J Immunol [PMID: 15749901]
  
  Details:
  Used the FITC form of this antibody.
• Chuang T, Ulevitch RJ. Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells. Biochim Biophys Acta. 2001 Mar. [PMID: 11267672]
• Muzio M, Natoli G, Saccani S et al. The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6). J Exp Med. 1998 Jun. [PMID: 9625770]
• Medzhitov R, Janeway CA Jr. Innate immunity: the virtues of a nonclonal system of recognition. Cell. 1997 Oct. [PMID: 9363937]

Bioinformatics

• Gene Symbol: TLR5
• Entrez:
  • 7100(Human)
  • 53791(Mouse)
  • 289337(Rat)