TLR5 Antibody (85B152.5)

Images

 
Western Blot: TLR5 Antibody (85B152.5) [NBP1-97728] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.
Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the FITC conjugate of NBP1-97728. Staining of TLR5 in 10^6 cells using 0.5 ugs of FITC-conjugated antibody: A) Intracellular flow testing of human ...read more
Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow analysis of TLR5 in Ramos cells using 1 ug of TLR5 antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype ...read more
Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow Analysis of TLR5 in 10^6 human lymphocytes using 0.5 ug of NB200-571. The shaded histogram represents cells without antibody, green represents ...read more
Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells ...read more
Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents ...read more

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, Flow, Flow-CS, Flow-IC, IHC-P
Clone
85B152.5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

TLR5 Antibody (85B152.5) Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5. It will cross-react with mouse TLR5 (NP_003259).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TLR5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Flow Cytometry 1-3 ug/10^6 cells in 100 ul
  • Immunohistochemistry-Paraffin
  • Flow (Cell Surface)
  • Flow (Intracellular)
Application Notes
Reported use in Flow Cytometry (cell surface): see Wong et al, 2007 for details. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 17570691). Use in Flow-cell surface and Flow-intracellular reported in scientific literature (PMID 24779433) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Agonist
Publications
Read Publications using
NBP1-97728 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for TLR5 Antibody (85B152.5)

  • MGC126430
  • MGC126431
  • SLEB1FLJ10052
  • TIL-3
  • TIL3Toll/interleukin-1 receptor-like protein 3
  • TLR5
  • toll-like receptor 5

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TLR5 Antibody (NBP1-97728)(30)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 6 applications: FLOW, Flow, Flow-CS, Flow-IC, IHC-P, WB.


Filter By Application
FLOW
(4)
Flow
(2)
Flow-CS
(7)
Flow-IC
(5)
IHC-P
(2)
WB
(4)
All Applications
Filter By Species
Human
(7)
Mouse
(4)
Canine
(1)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NBP1-97728 Applications Species
Mastorci K, Muraro E, Pasini E et al. Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma. PLoS ONE. 2016 April 29 [PMID: 27123851] (Flow, Human)

Details:
This citation used the FITC version of this antibody.
Flow Human
Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine)

Details:
Used the PE form of this antibody.
FLOW Canine
Kato K, Lillehoj EP, Kim KC. Pseudomonas aeruginosa stimulates tyrosine phosphorylation of and TLR5 association with the MUC1 cytoplasmic tail through EGFR activation. Inflamm. Res. Mar 1 2016 [PMID: 26645913] (WB, Human) WB Human
Mishra A, Brown AL, Yao X et al. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase Nat Commun Nat Commun [PMID: 25692509] (FLOW, Mouse)

Details:
This publication used the PE conjugated form of this antibody (Cat# NBP2-24959).
FLOW Mouse
Li Z, Younger K, Gartenhaus R et al. Inhibition of IRAK1/4 sensitizes T cell acute lymphoblastic leukemia to chemotherapies J. Clin. Invest. 2015 Mar 02 [PMID: 25642772] (WB, Human) WB Human
Bens M, Vimont S, Ben Mkaddem S et al. Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli Cell Microbiol 2014 Apr 29 [PMID: 24779433] (Flow-CS, Flow-IC, Mouse)

Details:
This citation used the PE version of this antibody.
Flow-CS, Flow-IC Mouse
Sawitzki B, Brunstein C, Meisel C et al. Prevention of Graft-versus-Host Disease by Adoptive T Regulatory Therapy Is Associated with Active Repression of Peripheral Blood Toll-Like Receptor 5 mRNA Expression. Biol Blood Marrow Transplant [PMID: 24184334] (FLOW, Human)

Details:
Used the FITC form of this antibody.
FLOW Human
Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013 Apr 8 [PMID: 23593278] (WB, Flow-IC)

Details:
Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
WB, Flow-IC
Feng T, Cong Y, Alexander K, Elson CO. Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells. PLoS One. 2012 [PMID: 22545147] (Flow-IC, Mouse)

Details:
Antibodies cited: TLR5 (IMG-663A), Flow-intracellular (DC cells derived from C57BL/6 mice): Fig 1c, d: lamina propria (LP) from the intestine and splenic DCs. The cells were doubled stained with a CD11b mAb and the IMG-663A TLR5 mAb. The LP DCs but not th
Flow-IC Mouse
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG et al. Toll-like receptor triggering in cord blood mesenchymal stem cells. J Cell Mol Med. 2009 Sep [PMID: 20196781] (Flow-CS, Human)

Details:
The following antibodies were used in flow (cell surface) cytometry: TLR5 (IMG-663A), TLR6 (IMG-304), TLR8 (IMG-321). Human mesenchymal stem cells, Fig 1C.
Flow-CS Human
Show All 30 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TLR5 Antibody (NBP1-97728) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-97728AF405
Alexa Fluor 488 Labeled NBP1-97728AF488
Alexa Fluor 647 Labeled NBP1-97728AF647
Alexa Fluor 700 Labeled NBP1-97728AF700
Allophycocyanin Labeled NBP1-97728APC
Biotin Labeled NBP1-97728B
DyLight 350 Labeled NBP1-97728UV
DyLight 405 Labeled NBP1-97728V
DyLight 488 Labeled NBP1-97728G
DyLight 550 Labeled NBP1-97728R
DyLight 650 Labeled NBP1-97728C
DyLight 680 Labeled NBP1-97728FR
DyLight 755 Labeled NBP1-97728IR
HRP Labeled NBP1-97728H
PE Labeled NBP2-24959
PerCP Labeled NBP1-97728PCP

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Blogs on TLR5.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

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Bioinformatics

Gene Symbol TLR5
Entrez