Survivin Antibody Pack

Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.

Knockdown Validated: Survivin Antibody Pack [NB100-911] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in the tumor tissues on day 19 using [NB500-201]. Scale bar, 50 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116022) licensed under a CC-BY licence.

Knockdown Validated: Survivin Antibody Pack [NB100-911] - Suppression of survivin expression by transfection of siRNA. RNA interference was performed in SW1353 and Hs 819.T, either for GFP as control or for survivin. A: A pronounced decrease of survivin protein levels was measured by immunoblotting in SW1353 and Hs819.T. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY licence. Survivin Antibody (60.11) [NB500-238]

Immunocytochemistry/Immunofluorescence: Survivin Antibody Pack [NB100-911] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using [NB500-201].

Immunohistochemistry: Survivin Antibody Pack [NB100-911] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237].

• Reactivity: Hu, Mu, Rt, Ca, Fe
• Applications: WB, ICC/IF, IHC, IHC-P, IP, Dual ISH-IHC, KD
• Host: Mouse

Survivin Antibody Pack Summary

• Immunogen: See individual datasheets.
• Marker: Astrocyte Marker
• Host: Mouse
• Gene: BIRC5

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot
• Application Notes: See individual datasheets for validated applications.

Reactivity Notes

See individual datasheets.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Notes

This pack contains 1 vial each of: NB500-201 (0.1 mL), NB500-237 (0.1 mL) and NB500-238 (0.1 mL).

Alternate Names for Survivin Antibody Pack

• API4
• apoptosis inhibitor 4
• Apoptosis inhibitor survivin
• baculoviral IAP repeat-containing 5
• Baculoviral IAP Repeat-Containing Protein 5
• BIRC5
• EPR-1
• IAP4
• survivin variant 3 alpha
• Survivin

Background

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Publications for Survivin Antibody Pack (NB100-911)(7)

Publications using NB100-911

• Suceveanu A, Micu I, Baltatescu G, et al. Overexpression of Survivin‑1, TAG‑72 and HERC5 in patients diagnosed with hepatocellular carcinoma in the Black Sea coast geographical area Experimental and Therapeutic Medicine 2021-01-26 [PMID: 33603891] (IHC-P, Human)
• Niedbala, W et al. Nitric oxide induces CD4+CD25+ Foxp3 regulatory T cells from CD4+CD25 T cells via p53, IL-2, and OX40. Proc Natl Acad Sci U S A 104(39):15478-83. 2007-09-25 [PMID: 17875988]
• Robles, Ana I et al. Schedule-dependent synergy between the heat shock protein 90 inhibitor 17-(dimethylaminoethylamino)-17-demethoxygeldanamycin and doxorubicin restores apoptosis to p53-mutant lymphoma cell lines. Clin Cancer Res;12(21):6547-56. 2006-11-01 [PMID: 17085670]
• Hahn, Joo-Yong et al. Beta-catenin overexpression reduces myocardial infarct size through differential effects on cardiomyocytes and cardiac fibroblasts. J Biol Chem;281(41):30979-89. 2006-10-13 [PMID: 16920707]
• Xia, F et al. Mitosis-independent survivin gene expression in vivo and regulation by p53. Cancer Res;66(7):3392-5. 2006-04-01 [PMID: 16585159]
• Paduano, F et al. Silencing of survivin gene by small interfering RNAs produces supra-additive growth suppression in combination with 17-allylamino-17-demethoxygeldanamycin in human prostate cancer cells. Mol Cancer Ther;5(1):179-86. 2006-01-01 [PMID: 16432177]
• Fortugno P, Beltrami E, Plescia et al. Regulation of survivin function by Hsp90. Proc Natl Acad Sci U S A. 2003-11-25 [PMID: 14614132]

FAQs for Survivin Antibody Pack (NB100-911). (Showing 1 - 4 of 4 FAQs).

1. Can I use this antibody with species other than those listed?
   • The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.
2. What does this "pack" come with?
   • This Survivin Antibody Pack comes with 1 vial each of: NB500-201 (0.1ml), NB500-237 (0.1ml) and NB500-238 (0.1ml). These are all Survivin antibodies.
3. What is the host, clonality, and clone numbers of the antibodies contained in the pack?
   • NB500-238 is a mouse monoclonal ab with clone number (60.11);  NB500-201 is a rabbit polyclonal ab, clone number is not applicable; NB500-237 is a mouse monoclonal ab with clone number (32.1).
4. What is the concentration of these antibodies?
   • NB500-238 has a concentration of 0.9 mg/ml, NB500-201 has a concentration of 1 mg/ml, and NB500-237 has a concentration of 1.9 mg/ml.

Bioinformatics

• Gene Symbol: BIRC5