Survivin Antibody Pack

Images

 
Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast ...read more
Genetic Strategies: Knockdown Validated: Survivin Antibody Pack [NB100-911] - Anti-tumor effects and functional evidence of sCA-survivin-siRNA in HCT116 and HT29 solid tumor models. Immunostaining of survivin in ...read more
Genetic Strategies: Knockdown Validated: Survivin Antibody Pack [NB100-911] - Suppression of survivin expression by transfection of siRNA. RNA interference was performed in SW1353 and Hs 819.T, either for GFP as ...read more
Immunocytochemistry/ Immunofluorescence: Survivin Antibody Pack [NB100-911] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. ...read more
Independent Antibodies: Immunohistochemistry: Survivin Antibody Pack [NB100-911] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power ...read more
Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237].

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, FeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, Dual ISH-IHC, KD
Host
Mouse

Order Details

Survivin Antibody Pack Summary

Immunogen
See individual datasheets.
Marker
Astrocyte Marker
Host
Mouse
Gene
BIRC5

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot
Application Notes
See individual datasheets for validated applications.
Publications
Read Publications using
NB100-911 in the following applications:

Reactivity Notes

See individual datasheets.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Kit Components

Notes

This pack contains 1 vial each of: NB500-201 (0.1 mL), NB500-237 (0.1 mL) and NB500-238 (0.1 mL).

Alternate Names for Survivin Antibody Pack

  • API4
  • apoptosis inhibitor 4
  • Apoptosis inhibitor survivin
  • baculoviral IAP repeat-containing 5
  • Baculoviral IAP Repeat-Containing Protein 5
  • BIRC5
  • EPR-1
  • IAP4
  • survivin variant 3 alpha
  • Survivin

Background

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-911
Species: Hu, Mu, Rt, Ca, Fe
Applications: WB, ICC/IF, IHC, IHC-P, IP, Dual ISH-IHC, KD

Publications for Survivin Antibody Pack (NB100-911)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Showing Publications 1 - 7 of 7.
Publications using NB100-911 Applications Species
Suceveanu A, Micu I, Baltatescu G, et al. Overexpression of Survivin‑1, TAG‑72 and HERC5 in patients diagnosed with hepatocellular carcinoma in the Black Sea coast geographical area Experimental and Therapeutic Medicine Jan 26 2021 [PMID: 33603891] (IHC-P, Human) IHC-P Human
Niedbala, W et al. Nitric oxide induces CD4+CD25+ Foxp3 regulatory T cells from CD4+CD25 T cells via p53, IL-2, and OX40. Proc Natl Acad Sci U S A;104(39):15478-83. 2007 Sep 25. [PMID: 17875988]
Robles AI, Wright MH, Gandhi B et al. Schedule-dependent synergy between the heat shock protein 90 inhibitor 17-(dimethylaminoethylamino)-17-demethoxygeldanamycin and doxorubicin restores apoptosis to p53-mutant lymphoma cell lines. Clin Cancer Res;12(21):6547-56. 2006 Nov 1. [PMID: 17085670]
Hahn, J et al. Beta-catenin overexpression reduces myocardial infarct size through differential effects on cardiomyocytes and cardiac fibroblasts. J Biol Chem;281(41):30979-89. 2006 Oct 13. [PMID: 16920707]
Xia, F et al. Mitosis-independent survivin gene expression in vivo and regulation by p53. Cancer Res;66(7):3392-5. 2006 Apr 1. [PMID: 16585159]
Paduano, F et al. Silencing of survivin gene by small interfering RNAs produces supra-additive growth suppression in combination with 17-allylamino-17-demethoxygeldanamycin in human prostate cancer cells. Mol Cancer Ther;5(1):179-86. 2006 Jan. [PMID: 16432177]
Fortugno, P et al. Regulation of survivin function by Hsp90. Proc Natl Acad Sci U S A5;100(24):13791-6. 2003 Nov 2. [PMID: 14614132]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Survivin Antibody Pack (NB100-911). (Showing 1 - 4 of 4 FAQs).

  1. Can I use this antibody with species other than those listed?
    • The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.
  2. What does this "pack" come with?
    • This Survivin Antibody Pack comes with 1 vial each of: NB500-201 (0.1ml), NB500-237 (0.1ml) and NB500-238 (0.1ml). These are all Survivin antibodies.
  3. What is the host, clonality, and clone numbers of the antibodies contained in the pack?
    • NB500-238 is a mouse monoclonal ab with clone number (60.11);  NB500-201 is a rabbit polyclonal ab, clone number is not applicable; NB500-237 is a mouse monoclonal ab with clone number (32.1).
  4. What is the concentration of these antibodies?
    • NB500-238 has a concentration of 0.9 mg/ml, NB500-201 has a concentration of 1 mg/ml, and NB500-237 has a concentration of 1.9 mg/ml.

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Blogs on Survivin. Showing 1-10 of 16 blog posts - Show all blog posts.


  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Survivin - an inhibitor of apoptosis that drives tumorigenesis and metastasis
Apoptosis is the tightly regulated process of programmed cell death. It plays an important role in normal physiologic development but has also been implicated in a number of diseases. Apoptosis is constantly downregulated by a family of anti-apop...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

Survivin: As long as I know how to live I know I will
Survivin is an anti-apoptotic protein from a large family with related members such as X-linked IAP, cIAP1 and cIAP2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates fundamental physiological events including the cell cycle, feta...  Read full blog post.

Survivin: Infographic
Survivin is involved in promoting cell proliferation and is an inhibitor of apoptosis. Survivin has a critical role in cancer proliferation and neural development. It may have an impact on neural cell proliferative responses following brain injury.L...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol BIRC5