Survivin Antibody (8E2)

Images

 
Immunohistochemistry: Survivin Antibody (8E2) [NB500-644] - Immunohistochemical analysis of Survivin in human breast cancer tissue using Survivin Antibody (8E2) [NB500-644] and DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: Survivin Antibody (8E2) [NB500-644] - Immunocytochemical analysis using Survivin Antibody (8E2) [NB500-644] was tested in HeLa cells with FITC (green). Nuclei were counterstained ...read more
Flow (Intracellular): Survivin Antibody (8E2) [NB500-644] - An intracellular stain was performed on Daudi cells with Survivin Antibody (8E2) [NB500-644] (blue) and a matched isotype control NBP2-27287 (orange). Cells ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P, In vitro, B/N
Clone
8E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Survivin Antibody (8E2) Summary

Immunogen
This Survivin Antibody (8E2) was developed against full length recombinant human Survivin [UniProt# O15392]
Localization
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton >spindle. Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BIRC5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • In vitro assay
  • Block/Neutralize
  • Flow (Intracellular)
Application Notes
For IHC, heat induced antigen retreval using citrate buffer is required. Blocking/Neutralizing (PMID: 14614132), ELISA (PMID: 9581817), and Flow Cytometry (PMID: 11313271) were reported in scientific literature.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB500-644 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Survivin Antibody (8E2)

  • API4
  • apoptosis inhibitor 4
  • Apoptosis inhibitor survivin
  • baculoviral IAP repeat-containing 5
  • Baculoviral IAP Repeat-Containing Protein 5
  • BIRC5
  • EPR-1
  • IAP4
  • survivin variant 3 alpha
  • Survivin

Background

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-201
Species: Hu, Mu, Rt, Ca, Fe, Gp, Ha
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Dual ISH-IHC, KD
NB100-56708
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
NB100-56098
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-56183
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-37387
Species: Hu
Applications: WB, ELISA, Flow, CyTOF-ready
NBP2-31106
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P, IP, CyTOF-ready, Flow-CS, ICC
NBP1-06579
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
NBP2-14214
Species: Hu
Applications: IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56104
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-Fr, IHC-P, IP
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP1-28566
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, Func, IHC, IHC-Fr
NBP2-22471
Species: Hu, Mu, Rt, Pm, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
NB500-213
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IP
AF1280
Species: Mu
Applications: WB, Simple Western, IHC
NBP2-24695
Species: Hu
Applications: WB, IHC, IHC-P
NB500-644
Species: Hu, Rt
Applications: ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P, In vitro, B/N

Publications for Survivin Antibody (NB500-644)(14)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: B/N, ELISA, FLOW, ICC/IF, IHC, IHC-P.


Filter By Application
B/N
(1)
ELISA
(2)
FLOW
(2)
ICC/IF
(2)
IHC
(1)
IHC-P
(4)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB500-644 Applications Species
Rosato A, Menin C, Boldrin D et al. Survivin expression impacts prognostically on NSCLC but not SCLC Lung Cancer 2012 Dec 3 [PMID: 23218791] (IHC, Human) IHC Human
Zeng SX, Li Y, Jin Y, Zhang Q, Keller DM, McQuaw CM, Barklis E, Stone S, Hoatlin M, Zhao Y, Lu H. Structure-specific recognition protein 1 facilitates microtubule growth and bundling required for mitosis. Mol Cell Biol30(4):935-47. 2010 Feb. [PMID: 19995907]
Fortugno P, Beltrami E, Plescia et al. Regulation of survivin function by Hsp90. Proc Natl Acad Sci U S A. 2003 Nov 25 [PMID: 14614132] (ELISA, B/N, Human) ELISA, B/N Human
Carvalho A, Carmena M, Sambade C, Earnshaw WC, Wheatley SP. Survivin is required for stable checkpoint activation in taxol-treated HeLa cells. J Cell Sci116(Pt 14):2987-98. 2003 Jul 15. [PMID: 12783991]
Fortugno P, Wall NR, Giodini A, O'Connor DS, Plescia J, Padgett KM, Tognin S, Marchisio PC, Altieri DC. Survivin exists in immunochemically distinct subcellular pools and is involved in spindle microtubule function. J Cell Sci115(Pt 3):575-85. 2002 Feb 1. [PMID: 11861764] (ICC/IF, Human) ICC/IF Human
Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC. INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis. Curr Biol11(11):886-90. 2001 Jun 5. [PMID: 11516652]
Kawasaki H, Toyoda M, Shinohara H, Okuda J, Watanabe I, Yamamoto T, Tanaka K, Tenjo T, Tanigawa N. Expression of survivin correlates with apoptosis, proliferation, and angiogenesis during human colorectal tumorigenesis. Cancer91(11):2026-32. 2001 Jun 1. [PMID: 11391581]
Granziero L, Ghia P, Circosta P, Gottardi D, Strola G, Geuna M, Montagna L, Piccoli P, Chilosi M, Caligaris-Cappio F. Survivin is expressed on CD40 stimulation and interfaces proliferation and apoptosis in B-cell chronic lymphocytic leukemia. Blood;97(9):2777-83. 2001 May 1. [PMID: 11313271] (IHC-P, FLOW, Human) IHC-P, FLOW Human
O'Connor DS, Grossman D, Plescia J, Li F, Zhang H, Villa A, Tognin S, Marchisio PC, Altieri DC. Regulation of apoptosis at cell division by p34cdc2 phosphorylation of survivin. Proc Natl Acad Sci U S A;97(24):13103-7. 2000 Nov 21. [PMID: 11069302]
Tanaka K, Iwamoto S, Gon G, Nohara T, Iwamoto M, Tanigawa N. Expression of survivin and its relationship to loss of apoptosis in breast carcinomas. Clin Cancer Res;6(1):127-34. 2000 Jan. [PMID: 10656440] (IHC-P) IHC-P
Show All 14 Publications.

Reviews for Survivin Antibody (NB500-644) (0)

There are no reviews for Survivin Antibody (NB500-644). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for Survivin Antibody (NB500-644): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Survivin Antibody (NB500-644). (Showing 1 - 2 of 2 FAQs).

  1. Can I use this antibody with species other than those listed?
    • The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.
  2. Do you have any recommendations for use in immunohistochemistry?
    • We recommend a dilution of 1:100 for immunohistochemistry. Heat induced antigen retrieval using citrate buffer is also required for this application.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB500-644AF350
Alexa Fluor 405 NB500-644AF405
Alexa Fluor 488 NB500-644AF488
Alexa Fluor 594 NB500-644AF594
Alexa Fluor 647 NB500-644AF647
Alexa Fluor 700 NB500-644AF700
Alexa Fluor 750 NB500-644AF750
Allophycocyanin NB500-644APC
Biotin NB500-644B
DyLight 350 NB500-644UV
DyLight 405 NB500-644V
DyLight 488 NB500-644G
DyLight 550 NB500-644R
DyLight 650 NB500-644C
DyLight 680 NB500-644FR
DyLight 755 NB500-644IR
FITC NB500-644F
HRP NB500-644H
Janelia Fluor 549 NB500-644JF549
Janelia Fluor 646 NB500-644JF646
PE NB500-644PE
PerCP NB500-644PCP

Additional Survivin Products

Bioinformatics Tool for Survivin Antibody (NB500-644)

Discover related pathways, diseases and genes to Survivin Antibody (NB500-644). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Survivin Antibody (NB500-644)

Discover more about diseases related to Survivin Antibody (NB500-644).
 

Pathways for Survivin Antibody (NB500-644)

View related products by pathway.

PTMs for Survivin Antibody (NB500-644)

Learn more about PTMs related to Survivin Antibody (NB500-644).
 

Research Areas for Survivin Antibody (NB500-644)

Find related products by research area.

Blogs on Survivin. Showing 1-10 of 15 blog posts - Show all blog posts.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of...  Read full blog post.

Survivin - an inhibitor of apoptosis that drives tumorigenesis and metastasis
Apoptosis is the tightly regulated process of programmed cell death. It plays an important role in normal physiologic development but has also been implicated in a number of diseases. Apoptosis is constantly downregulated by a family of anti-apop...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

Survivin: As long as I know how to live I know I will
Survivin is an anti-apoptotic protein from a large family with related members such as X-linked IAP, cIAP1 and cIAP2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates fundamental physiological events including the cell cycle, feta...  Read full blog post.

Survivin: Infographic
Survivin is involved in promoting cell proliferation and is an inhibitor of apoptosis. Survivin has a critical role in cancer proliferation and neural development. It may have an impact on neural cell proliferative responses following brain injury.L...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Survivin Antibody (8E2) and receive a gift card or discount.

Bioinformatics

Gene Symbol BIRC5
Entrez
Uniprot