SOX2 Antibody (245610) [Unconjugated]

Images

 
SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, ...read more
Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat ...read more
E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, CyTOF-reported, Flow, ICC/IF
Clone
245610
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

SOX2 Antibody (245610) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Specificity
Detects human, mouse, and rat SOX2 in Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SOX2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 4 ug/mL
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 5 Reviews rated 4.4
using
MAB2018 in the following applications:

Publications
Read Publications using
MAB2018 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX2 Antibody (245610) [Unconjugated]

  • ANOP3
  • MCOPS3
  • MGC2413
  • SOX2
  • SRY (sex determining region Y)-box 2
  • SRY-related HMG-box gene 2
  • transcription factor SOX2
  • transcription factor SOX-2

Background

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX2 Antibody (MAB2018)(78)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 14 applications: Flow, ICC, ICC Fluorescence, IF, IHC, IHC - Fresh, IHC - Frozen, IHC - PFA fixed, IHC - Paraffin embedded, IHC Fresh, IHC Frozen, IHC OCT-embedded, IHC Paraffin-embedded, WB.


Filter By Application
Flow
(2)
ICC
(31)
ICC Fluorescence
(1)
IF
(3)
IHC
(9)
IHC - Fresh
(2)
IHC - Frozen
(1)
IHC - PFA fixed
(1)
IHC - Paraffin embedded
(2)
IHC Fresh
(4)
IHC Frozen
(2)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(2)
WB
(15)
All Applications
Filter By Species
Human
(45)
Mouse
(25)
Rat
(4)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 78. Show All 78 Publications.
Publications using MAB2018 Applications Species
S Kwak, TW Kim, BH Kang, JH Kim, JS Lee, HT Lee, IY Hwang, J Shin, JH Lee, EJ Cho, HD Youn Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation Nucleic Acids Res., 2018;0(0):. 2018 [PMID: 29846698] (WB,, Mouse) WB Mouse
O Le, L Palacio, G Bernier, I Batinic-Ha, G Hickson, C Beauséjour INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29706499] (IHC,, Mouse) IHC Mouse
WA Pastor, W Liu, D Chen, J Ho, R Kim, TJ Hunt, A Lukianchik, X Liu, JM Polo, SE Jacobsen, AT Clark TFAP2C regulates transcription in human naive pluripotency by opening enhancers Nat. Cell Biol., 2018;20(5):553-564. 2018 [PMID: 29695788] (WB,, Human) WB Human
M Mura, M Ginevrino, R Zappatore, F Pisano, M Boni, S Castellett, L Crotti, EM Valente, PJ Schwartz, M Gnecchi Generation of the human induced pluripotent stem cell (hiPSC) line PSMi003-A from a patient affected by an autosomal recessive form of Long QT Syndrome type 1 Stem Cell Res, 2018;29(0):170-173. 2018 [PMID: 29684900] (ICC,, Human) ICC Human
M Mura, YK Lee, M Ginevrino, R Zappatore, F Pisano, M Boni, F Dagradi, L Crotti, EM Valente, PJ Schwartz, HF Tse, M Gnecchi Generation of the human induced pluripotent stem cell (hiPSC) line PSMi002-A from a patient affected by the Jervell and Lange-Nielsen syndrome and carrier of two compound heterozygous mutations on the KCNQ1 gene Stem Cell Res, 2018;29(0):157-161. 2018 [PMID: 29677589] (ICC,, Human) ICC Human
JY Lee, SH Han, MH Park, B Baek, IS Song, MK Choi, Y Takuwa, H Ryu, SH Kim, X He, EH Schuchman, JS Bae, HK Jin Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease Nat Commun, 2018;9(1):1479. 2018 [PMID: 29662056] (ICC,, Mouse) ICC Mouse
NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation Cell Stem Cell, 2018;22(4):543-558.e12. 2018 [PMID: 29625069] (ICC,, Mouse) ICC Mouse
C Aaberg-Jes, MD Sørensen, ALSA Matos, JM Moreira, N Brünner, A Knudsen, BW Kristensen Co-expression of TIMP-1 and its cell surface binding partner CD63 in glioblastomas BMC Cancer, 2018;18(1):270. 2018 [PMID: 29523123] (IHC,, Human) IHC Human
M Naumann, A Pal, A Goswami, X Lojewski, J Japtok, A Vehlow, M Naujock, R Günther, M Jin, N Stanslowsk, P Reinhardt, J Sternecker, M Frickenhau, F Pan-Montoj, E Storkebaum, I Poser, A Freischmid, JH Weishaupt, K Holzmann, D Troost, AC Ludolph, TM Boeckers, S Liebau, S Petri, N Cordes, AA Hyman, F Wegner, SW Grill, J Weis, A Storch, A Hermann Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation Nat Commun, 2018;9(1):335. 2018 [PMID: 29362359] (ICC,, Human) ICC Human
A Alastrue-A, FJ Rodriguez-, EL Mocholi, F De Giorgio, S Erceg, V Moreno-Man FM19G11 and Ependymal Progenitor/Stem Cell Combinatory Treatment Enhances Neuronal Preservation and Oligodendrogenesis after Severe Spinal Cord Injury Int J Mol Sci, 2018;19(1):. 2018 [PMID: 29315225] (WB,, Rat) WB Rat
Show All 78 Publications.

Reviews for SOX2 Antibody (MAB2018) (5) 4.45

Average Rating: 4.4
(Based on 5 reviews)
We have 5 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using MAB2018:
Filter by Applications
IF
(5)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Rat
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Rat 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22791629
SpeciesRat

Blocking

Blocking DetailsSee PMID 22791629

Details

Detection NotesSee PMID 22791629

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22791629
 
reviewed by:
Anonymous
IF Human 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19824823
SpeciesHuman

Blocking

Blocking DetailsSee PMID 19824823

Details

Detection NotesSee PMID 19824823

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 19824823
 
reviewed by:
Anonymous
IF Mouse 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19825428
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19825428

Details

Detection NotesSee PMID 19825428

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 19825428
 
reviewed by:
Anonymous
IF Human 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23658023

Details

Detection NotesSee PMID 23658023

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23658023
 
reviewed by:
Anonymous
IF Other 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22573702
SpeciesOther

Blocking

Blocking DetailsSee PMID 22573702

Details

Detection NotesSee PMID 22573702

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22573702

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOX2 Antibody (MAB2018) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

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Blogs on SOX2. Showing 1-10 of 441 blog posts - Show all blog posts.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Showing 1-10 of 441 blog posts - Show all blog posts.

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Recent Reviews

4.4
5
2
4
3
3
0
2
0
1
0

 
Anonymous
02/16/2015
Application: IF
Species: Rat

 
Anonymous
02/16/2015
Application: IF
Species: Human

 
Anonymous
02/16/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol SOX2
Entrez
Uniprot