SOX2 Antibody (245610) [Unconjugated]

Images

 
SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, ...read more
Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat ...read more
E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, CyTOF-reported, Flow, ICC/IF
Clone
245610
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

SOX2 Antibody (245610) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Specificity
Detects human, mouse, and rat SOX2 in Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SOX2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 4 ug/mL
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 5 Reviews rated 4.4
using
MAB2018 in the following applications:

Publications
Read Publications using
MAB2018 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX2 Antibody (245610) [Unconjugated]

  • ANOP3
  • MCOPS3
  • MGC2413
  • SOX2
  • SRY (sex determining region Y)-box 2
  • SRY-related HMG-box gene 2
  • transcription factor SOX2
  • transcription factor SOX-2

Background

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX2 Antibody (MAB2018)(81)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Rhesus Macaque.

We have publications tested in 14 applications: Flow, ICC, ICC Fluorescence, IF, IHC, IHC - Fresh, IHC - Frozen, IHC - PFA fixed, IHC - Paraffin embedded, IHC Fresh, IHC Frozen, IHC OCT-embedded, IHC Paraffin-embedded, WB.


Filter By Application
Flow
(2)
ICC
(34)
ICC Fluorescence
(1)
IF
(3)
IHC
(9)
IHC - Fresh
(2)
IHC - Frozen
(1)
IHC - PFA fixed
(1)
IHC - Paraffin embedded
(2)
IHC Fresh
(4)
IHC Frozen
(2)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(2)
WB
(15)
All Applications
Filter By Species
Human
(46)
Mouse
(25)
Rat
(5)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Rhesus Macaque
(1)
All Species
Showing Publications 1 - 10 of 81. Show All 81 Publications.
Publications using MAB2018 Applications Species
R Nakai, M Ohnuki, K Kuroki, H Ito, H Hirai, R Kitajima, T Fujimoto, M Nakagawa, W Enard, M Imamura Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata) Sci Rep, 2018;8(1):12187. 2018 [PMID: 30111816] (ICC,, Rhesus Macaque) ICC Rhesus Macaque
SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo J Neuroinflammation, 2018;15(1):226. 2018 [PMID: 30103769] (ICC,, Rat) ICC Rat
X Gao, JJ Yourick, RL Sprando Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel Stem Cell Res, 2018;31(0):193-196. 2018 [PMID: 30099336] (ICC,, Human) ICC Human
S Kwak, TW Kim, BH Kang, JH Kim, JS Lee, HT Lee, IY Hwang, J Shin, JH Lee, EJ Cho, HD Youn Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation Nucleic Acids Res., 2018;0(0):. 2018 [PMID: 29846698] (WB,, Mouse) WB Mouse
O Le, L Palacio, G Bernier, I Batinic-Ha, G Hickson, C Beauséjour INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29706499] (IHC,, Mouse) IHC Mouse
WA Pastor, W Liu, D Chen, J Ho, R Kim, TJ Hunt, A Lukianchik, X Liu, JM Polo, SE Jacobsen, AT Clark TFAP2C regulates transcription in human naive pluripotency by opening enhancers Nat. Cell Biol., 2018;20(5):553-564. 2018 [PMID: 29695788] (WB,, Human) WB Human
M Mura, M Ginevrino, R Zappatore, F Pisano, M Boni, S Castellett, L Crotti, EM Valente, PJ Schwartz, M Gnecchi Generation of the human induced pluripotent stem cell (hiPSC) line PSMi003-A from a patient affected by an autosomal recessive form of Long QT Syndrome type 1 Stem Cell Res, 2018;29(0):170-173. 2018 [PMID: 29684900] (ICC,, Human) ICC Human
M Mura, YK Lee, M Ginevrino, R Zappatore, F Pisano, M Boni, F Dagradi, L Crotti, EM Valente, PJ Schwartz, HF Tse, M Gnecchi Generation of the human induced pluripotent stem cell (hiPSC) line PSMi002-A from a patient affected by the Jervell and Lange-Nielsen syndrome and carrier of two compound heterozygous mutations on the KCNQ1 gene Stem Cell Res, 2018;29(0):157-161. 2018 [PMID: 29677589] (ICC,, Human) ICC Human
JY Lee, SH Han, MH Park, B Baek, IS Song, MK Choi, Y Takuwa, H Ryu, SH Kim, X He, EH Schuchman, JS Bae, HK Jin Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease Nat Commun, 2018;9(1):1479. 2018 [PMID: 29662056] (ICC,, Mouse) ICC Mouse
NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation Cell Stem Cell, 2018;22(4):543-558.e12. 2018 [PMID: 29625069] (ICC,, Mouse) ICC Mouse
Show All 81 Publications.

Reviews for SOX2 Antibody (MAB2018) (5) 4.45

Average Rating: 4.4
(Based on 5 reviews)
We have 5 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using MAB2018:
Filter by Applications
IF
(5)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Rat
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Rat 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22791629
SpeciesRat

Blocking

Blocking DetailsSee PMID 22791629

Details

Detection NotesSee PMID 22791629

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22791629
 
reviewed by:
Anonymous
IF Human 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19824823
SpeciesHuman

Blocking

Blocking DetailsSee PMID 19824823

Details

Detection NotesSee PMID 19824823

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 19824823
 
reviewed by:
Anonymous
IF Mouse 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19825428
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19825428

Details

Detection NotesSee PMID 19825428

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 19825428
 
reviewed by:
Anonymous
IF Human 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23658023

Details

Detection NotesSee PMID 23658023

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23658023
 
reviewed by:
Anonymous
IF Other 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22573702
SpeciesOther

Blocking

Blocking DetailsSee PMID 22573702

Details

Detection NotesSee PMID 22573702

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22573702

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SOX2 Antibody (MAB2018) (0)

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Blogs on SOX2.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

SOX2: an Important Stem Cell Transcription Factor
SOX2 is a transcription factor that is expressed by self-renewing and multipotent stem cells of the embryonic neuroepithelium. Sox-2 was found to be expressed by dividing neural progenitor cells. Constitutive expression of SOX2 has also been shown to...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

The Sox2 Antibody Aids Brain Cancer Research
The Sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. Recently, Sox2 antibody preparations identified the Sox2 protein as a marker for malignant neural gliomas. We at Novus Biologicals offer a wide variety of highly...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Recent Reviews

4.4
5
2
4
3
3
0
2
0
1
0

 
Anonymous
02/16/2015
Application: IF
Species: Rat

 
Anonymous
02/16/2015
Application: IF
Species: Human

 
Anonymous
02/16/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol SOX2
Entrez
Uniprot