SOX2 Antibody (245610) [Unconjugated]

Images

 
Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat ...read more
Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was ...read more
E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Mouse ...read more
NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, ...read more
SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, CyTOF-reported, Flow, ICC/IF
Clone
245610
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

SOX2 Antibody (245610) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Specificity
Detects human, mouse, and rat SOX2 in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SOX2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 4 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 5 Reviews rated 4.4
using
MAB2018 in the following applications:

Publications
Read Publications using
MAB2018 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX2 Antibody (245610) [Unconjugated]

  • ANOP3
  • MCOPS3
  • MGC2413
  • SOX2
  • SRY (sex determining region Y)-box 2
  • SRY-related HMG-box gene 2
  • transcription factor SOX2
  • transcription factor SOX-2

Background

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, Flow, ICC/IF

Publications for SOX2 Antibody (MAB2018)(144)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Pan troglodytes (Chimpanzee), Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 8 applications: ChIP, Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ChIP
(1)
Flow Cytometry
(8)
ICC
(72)
IF
(1)
IHC
(24)
IHC-Fr
(10)
IHC-P
(7)
Western Blot
(18)
All Applications
Filter By Species
Human
(97)
Mouse
(34)
Rat
(7)
Pan troglodytes (Chimpanzee)
(1)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 144. Show All 144 Publications.
Publications using MAB2018 Applications Species
BM Brooks, M Pradhan, YS Cheng, K Gorshkov, A Farkhondeh, CZ Chen, J Beers, C Liu, K Baumgaerte, S Rodems, W Zheng Generation of an induced pluripotent stem cell line (TRNDi031-A) from a patient with Alagille syndrome type 1 carrying a heterozygous p. C312X (c. 936�T�>�A) mutation in JAGGED-1 Stem Cell Research, 2021;54(0):102447. 2021 [PMID: 34198154] (ICC, Human) ICC Human
L Ma, M Schmidt, EM Morrow Human iPSC lines from a Christianson syndrome patient with NHE6 W523X mutation, a biologically-related control, and CRISPR/Cas9 gene-corrected isogenic controls Stem Cell Research, 2021;54(0):102435. 2021 [PMID: 34182254] (ICC, Human) ICC Human
M Notaras, A Lodhi, E Barrio-Alo, C Foord, T Rodrick, D Jones, H Fang, D Greening, D Colak Neurodevelopmental signatures of narcotic and neuropsychiatric risk factors in 3D human-derived forebrain organoids Molecular Psychiatry, 2021;0(0):. 2021 [PMID: 34158620] (IHC, Human) IHC Human
X Huang, A Roeder, R Li, J Beers, C Liu, J Zou, PB Yu, W Zheng Generation of an induced pluripotent stem cell line (TRNDi012-B) from Fibrodysplasia Ossificans Progressiva (FOP) patient carrying a heterozygous mutation c. 617G�>�A in the ACVR1 gene Stem Cell Research, 2021;54(0):102424. 2021 [PMID: 34139597] (Flow Cytometry, Human) Flow Cytometry Human
MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration Science Advances, 2021;7(21):. 2021 [PMID: 34138734] (Flow Cytometry, Human) Flow Cytometry Human
F Xiong, R Wang, JH Lee, S Li, SF Chen, Z Liao, LA Hasani, PT Nguyen, X Zhu, J Krakowiak, DF Lee, L Han, KL Tsai, Y Liu, W Li RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability Cell Research, 2021;0(0):. 2021 [PMID: 34108665] (ICC, Human) ICC Human
BM Brooks, CD Yeh, J Beers, C Liu, YS Cheng, K Gorshkov, J Zou, W Zheng, CZ Chen Generation of an induced pluripotent stem cell line (TRNDi030-A) from a patient with Farber disease carrying a homozygous p. Y36C (c. 107 A>G) mutation in ASAH1 Stem Cell Research, 2021;53(0):102387. 2021 [PMID: 34088014] (Flow Cytometry, Human) Flow Cytometry Human
W Zhu, YS Cheng, M Xu, A Farkhondeh, J Beers, J Zou, C Liu, K Baumgaerte, S Rodems, W Zheng Generation of Alagille syndrome derived induced pluripotent stem cell line carrying heterozygous mutation in the JAGGED-1 gene at splicing site (Chr20: 10,629,709C>A) before exon 11 Stem Cell Research, 2021;53(0):102366. 2021 [PMID: 34087995] (ICC, Human) ICC Human
TP Silva, CA Pereira, AC Raposo, AR Oliveira, M Arez, JMS Cabral, I Milagre, M Carmo-Fons, STD Rocha Generation and characterization of induced pluripotent stem cells heterozygous for the Portuguese BRCA2 founder mutation Stem Cell Research, 2021;53(0):102364. 2021 [PMID: 34087993] (ICC, Human) ICC Human
YC Lin, MS Kumar, N Ramesh, EN Anderson, AT Nguyen, B Kim, S Cheung, JA McDonough, WC Skarnes, R Lopez-Gonz, JE Landers, NL Fawzi, IRA Mackenzie, EB Lee, JA Nickerson, D Grunwald, UB Pandey, DA Bosco Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway Nature Neuroscience, 2021;0(0):. 2021 [PMID: 34059832] (ICC, Human) ICC Human
Show All 144 Publications.

Reviews for SOX2 Antibody (MAB2018) (5) 4.45

Average Rating: 4.4
(Based on 5 reviews)
We have 5 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using MAB2018:
Filter by Applications
IF
(5)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Rat
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Rat 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22791629
SpeciesRat

Blocking

Blocking DetailsSee PMID 22791629

Details

Detection NotesSee PMID 22791629

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22791629
  5
reviewed by:
Verified Customer
IF Human 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19824823
SpeciesHuman

Blocking

Blocking DetailsSee PMID 19824823

Details

Detection NotesSee PMID 19824823

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 19824823
  4
reviewed by:
Verified Customer
IF Mouse 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19825428
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19825428

Details

Detection NotesSee PMID 19825428

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 19825428
  4
reviewed by:
Verified Customer
IF Human 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23658023

Details

Detection NotesSee PMID 23658023

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23658023
  4
reviewed by:
Verified Customer
IF Other 02/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22573702
SpeciesOther

Blocking

Blocking DetailsSee PMID 22573702

Details

Detection NotesSee PMID 22573702

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22573702

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOX2 Antibody (MAB2018) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC2018U
Alexa Fluor 405 IC2018V
Alexa Fluor 488 IC2018G
Alexa Fluor 532 FAB2018X
Alexa Fluor 594 IC2018T
Alexa Fluor 647 IC2018R
Alexa Fluor 700 IC2018N
Alexa Fluor 750 IC2018S
Allophycocyanin IC2018A
Biotin FAB2018B
DyLight 350 FAB2018D
DyLight 405 FAB2018E
DyLight 488 FAB2018K
DyLight 550 FAB2018L
DyLight 594 FAB2018M
DyLight 650 FAB2018W
DyLight 680 FAB2018Y
DyLight 755 FAB2018Z
FITC FAB2018F
HRP FAB2018H
Janelia Fluor 549 FAB2018I
Janelia Fluor 646 FAB2018J
Phycoerythrin IC2018P
Phycoerythrin IC2018P

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Bioinformatics Tool for SOX2 Antibody (MAB2018)

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Blogs on SOX2. Showing 1-10 of 12 blog posts - Show all blog posts.


  Read full blog post.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

SOX2: an Important Stem Cell Transcription Factor
SOX2 is a transcription factor that is expressed by self-renewing and multipotent stem cells of the embryonic neuroepithelium. Sox-2 was found to be expressed by dividing neural progenitor cells. Constitutive expression of SOX2 has also been shown to ...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Recent Reviews

4.4
5
2
4
3
3
0
2
0
1
0

 
Verified Customer
02/16/2015
Application: IF
Species: Rat

 
Verified Customer
02/16/2015
Application: IF
Species: Human

 
Verified Customer
02/16/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol SOX2
Uniprot