SMN Antibody (2B1)


Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were more
Western Blot: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were more
Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - scIF of control (f) and etv5b MO (g) cells from pmnx1:eGFP embryos. H2B, GFP and Smn signals are shown in Z-projected confocal sections. GFP+ more
Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were more
Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Neuro2a cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and more
Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against DyLight 488 (Green). Actin nuclei were counterstained against Phalloidin more
Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN on mouse brain using NB100-1936.
Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Ntera2 cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and more

Product Details

Reactivity Hu, Mu, Rt, Pm, Xp, ZeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
1.0 mg/ml

Order Details

SMN Antibody (2B1) Summary

Purified recombinant His6-tagged human SMN protein. [Swiss-Prot# Q16637]
Cytoplasm. Nucleus > Gem, localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA reported in scientific literature (PMID 23973875)
  • Flow Cytometry reported in scientific literature (PMID 30102724)
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2 ug/ml
Application Notes
This SMN Antibody (2B1) is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation, and Western blot where a band can be seen at ~ 35 kDa.
HeLa Whole Cell Lysate
Read Publications using
NB100-1936 in the following applications:

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 27273160).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for SMN Antibody (2B1)

  • Gemin-1
  • Kugelberg-Welander disease)
  • SMNC
  • survival motor neuron protein
  • survival of motor neuron 1, telomeric


Spinal muscular atrophy (SMA) is a common fatal autosomal recessive disorder characterized by the degeneration of motor neurons and muscular atrophy. A gene called Survival of Motor Neurons (SMN) has been identified as the determining gene in SMA and is deleted or mutated in greater than 98% of SMA patients. The SMN protein localizes to both the cytoplasm and the nucleus. In the nucleus, SMN has been found to localize in a nuclear structure termed gems for Gemini of coiled bodies, which contain high levels of small ribonucleoproteins (snRNPs). SMN forms complexes with proteins including SIP1, Gemin 2-7 and several others known to be involved in the biogenesis of snRNPs. SMN antibodies may serve as a useful research tool to study SMN complexes and gems, and their role in pre-RNA processing and RNA metabolism.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
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Applications: WB
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Applications: IHC, IHC-P, PEP-ELISA, WB
Species: Mu
Applications: WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
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Applications: Flow, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, KD, WB
Species: Hu
Applications: BA
Species: Hu
Applications: Flow, ICC/IF, PA

Publications for SMN Antibody (NB100-1936)(23)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Xenopus, Zebrafish.

We have publications tested in 6 applications: ELISA, FLOW, ICC/IF, IF/IHC, IP, WB.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB100-1936 Applications Species
Wu SH, Lu IC, Yang SM et al. Spinal Irisin Gene Delivery Attenuates Burn Injury-Induced Muscle Atrophy by Promoting Axonal Myelination and Innervation of Neuromuscular Junctions International journal of molecular sciences 2022-12-14 [PMID: 36555538] (WB, Rat) WB Rat
Stewart LM, Gerner L, Rettel M Et al. CaMKK2 facilitates Golgi-associated vesicle trafficking to sustain cancer cell proliferation Cell death & disease 2021-11-01 [PMID: 34725334]
Nishibe M, Toyoda H, Hiraga SI Et al. Synaptic and Genetic Bases of Impaired Motor Learning Associated with Modified Experience-Dependent Cortical Plasticity in Heterozygous Reeler Mutants Cerebral cortex (New York, N.Y. : 1991) 2021-07-31 [PMID: 34339488] (IF/IHC, Mouse) IF/IHC Mouse
Otsuki N, Arakawa R, Kaneko K et al. A new biomarker candidate for spinal muscular atrophy: Identification of a peripheral blood cell population capable of monitoring the level of survival motor neuron protein PLoS ONE. 2018-08-13 [PMID: 30102724] (FLOW, Human)

Citation using the Alexa Fluor 488 form of this antibody.
FLOW Human
Chen HY, Lin YN, Chen WC et al. Urethral proteomic analysis in ovariectomized mice administered 17b-oestradiol replacement therapy. J Obstet Gynaecol. 2017-03-28 [PMID: 28350532] (Mouse) Mouse
Kobayashi M, Chandrasekhar A, Cheng C et al. Diabetic polyneuropathy, sensory neurons, nuclear structure and spliceosome alterations: a role for CWC22. Dis Model Mech. 2017-03-01 [PMID: 28250049]
Spiro Z, Koh A, Tay S et al. Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep 2016-06-09 [PMID: 27273160] (ICC/IF, Zebrafish)

This publication used the AF647 conjugated form of this antibody (NB100-1936AF647)
ICC/IF Zebrafish
Kassim SH, Jordan J, Schreiter J et al. Systematic identification of novel SLE related autoantibodies responsible for type I IFN production in human plasmacytoid dendritic cells. Cell Immunol. 2013-08-06 [PMID: 23973875] (ELISA, Human) ELISA Human
Yong J, Golembe TJ, Battle DJ, Pellizzoni L, Dreyfuss G. snRNAs contain specific SMN-binding domains that are essential for snRNP assembly. Mol Cell Biol;24(7):2747-56. 2004-04-01 [PMID: 15024064] (IP, WB, Xenopus, Human) IP, WB Xenopus, Human
Nelson PT, Hatzigeorgiou AG, Mourelatos Z. miRNP:mRNA association in polyribosomes in a human neuronal cell line. RNA. 2004-03-01 [PMID: 14970384] (WB, Human) WB Human
Show All 23 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMN Antibody (NB100-1936). (Showing 1 - 1 of 1 FAQs).

  1. Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.
    • We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol SMN1