SMN Antibody (2B1)

Images

 
Western Blot: SMN Antibody (2B1) [NB100-1936] - Western blot analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12 and 7) COS7 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against Dylight 488 (Green). Actin nuclei were counterstained against Phalloidin ...read more
Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - Immunohistochemical analysis of SMN on mouse brain using NB100-1936.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, XpSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
2B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SMN Antibody (2B1) Summary

Immunogen
Purified recombinant His6-tagged human SMN protein. [Swiss-Prot# Q16637]
Localization
Cytoplasm. Nucleus > gem. Note: Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SMN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This SMN Antibody (2B1) is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation and Western blot where a band can be seen at ~ 35 kDa. Use in ELISA reported in scientific literature (PMID 23973875)
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB100-1936 in the following applications:

  • 1 publication
  • 6 publications
  • IP
    10 publications
  • WB
    10 publications

Reactivity Notes

Human, mouse, rat, monkey and Xenopus. Other species not tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for SMN Antibody (2B1)

  • Gemin-1
  • Kugelberg-Welander disease)
  • SMNC
  • survival motor neuron protein
  • survival of motor neuron 1, telomeric

Background

Spinal muscular atrophy (SMA) is a common fatal autosomal recessive disorder characterized by the degeneration of motor neurons and muscular atrophy. A gene called Survival of Motor Neurons (SMN) has been identified as the determining gene in SMA and is deleted or mutated in greater than 98% of SMA patients. The SMN protein localizes to both the cytoplasm and the nucleus. In the nucleus, SMN has been found to localize in a nuclear structure termed gems for Gemini of coiled bodies, which contain high levels of small ribonucleoproteins (snRNPs). SMN forms complexes with proteins including SIP1, Gemin 2-7 and several others known to be involved in the biogenesis of snRNPs. SMN antibodies may serve as a useful research tool to study SMN complexes and gems, and their role in pre-RNA processing and RNA metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMN Antibody (NB100-1936)(19)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Xenopus, Zebrafish.

We have publications tested in 4 applications: ELISA, ICC/IF, IP, WB.


Filter By Application
ELISA
(1)
ICC/IF
(6)
IP
(10)
WB
(10)
All Applications
Filter By Species
Human
(14)
Mouse
(3)
Rat
(1)
Xenopus
(3)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-1936 Applications Species
Chen HY, Lin YN, Chen WC et al. Urethral proteomic analysis in ovariectomized mice administered 17b-oestradiol replacement therapy. J Obstet Gynaecol. Mar 28 2017 [PMID: 28350532] (Mouse) Mouse
Kobayashi M, Chandrasekhar A, Cheng C et al. Diabetic polyneuropathy, sensory neurons, nuclear structure and spliceosome alterations: a role for CWC22. Dis Model Mech. 2017 Mar 01 [PMID: 28250049]
Spiro Z, Koh A, Tay S et al. Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep 2016 June 9 [PMID: 27273160] (ICC/IF, Zebrafish)

Details:
This publication used the AF647 conjugated form of this antibody (NB100-1936AF647)
ICC/IF Zebrafish
Kassim SH, Jordan J, Schreiter J et al. Systematic identification of novel SLE related autoantibodies responsible for type I IFN production in human plasmacytoid dendritic cells. Cell Immunol. 2013 Aug 6 [PMID: 23973875] (ELISA, Human) ELISA Human
Yong J, Golembe TJ, Battle DJ, Pellizzoni L, Dreyfuss G. snRNAs contain specific SMN-binding domains that are essential for snRNP assembly. Mol Cell Biol;24(7):2747-56. 2004 Apr. [PMID: 15024064] (WB, IP, Xenopus, Human) WB, IP Xenopus, Human
Nelson PT, Hatzigeorgiou AG, Mourelatos Z. miRNP:mRNA association in polyribosomes in a human neuronal cell line. RNA;10(3):387-94. 2004 Mar. [PMID: 14970384] (WB, Human) WB Human
Massenet S, Pellizzoni L, Paushkin S, Mattaj IW, Dreyfuss G. The SMN complex is associated with snRNPs throughout their cytoplasmic assembly pathway. Mol Cell Biol;22(18):6533-41. 2002 Sep. [PMID: 12192051] (IP, Human) IP Human
Paushkin S, Gubitz AK, Massenet S, Dreyfuss G. The SMN complex, an assemblyosome of ribonucleoproteins. Curr Opin Cell Biol;14(3):305-12. 2002 Jun. [PMID: 12067652] (IP, Human) IP Human
Pellizzoni L, Baccon J, Rappsilber J, Mann M, Dreyfuss G. Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component. J Biol Chem;277(9):7540-5. 2002 Mar 1. [PMID: 11748230] (IP, Human) IP Human
Gubitz AK, Mourelatos Z, Abel L, Rappsilber J, Mann M, Dreyfuss G. Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins. J Biol Chem;277(7):5631-6. 2002 Feb 15. [PMID: 11714716] (WB, IP, Human) WB, IP Human
Show All 19 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMN Antibody (NB100-1936). (Showing 1 - 1 of 1 FAQs).

  1. Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.
    • We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1936AF405
Alexa Fluor 488 Labeled NB100-1936AF488
Alexa Fluor 647 Labeled NB100-1936AF647
Alexa Fluor 700 Labeled NB100-1936AF700
DyLight 350 Labeled NB100-1936UV
DyLight 405 Labeled NB100-1936V
DyLight 488 Labeled NB100-1936G
DyLight 680 Labeled NB100-1936FR
DyLight 755 Labeled NB100-1936IR
FITC Labeled NB100-1936F

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Bioinformatics

Gene Symbol SMN1
Entrez
Uniprot