Western Blot: SMN Antibody (2B1) [NB100-1936] - Western blot analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12 and 7) COS7 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against Dylight 488 (Green). Actin nuclei were counterstained against Phalloidin ...read more
Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - Immunohistochemical analysis of SMN on mouse brain using NB100-1936.
This SMN Antibody (2B1) is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation and Western blot where a band can be seen at ~ 35 kDa. Use in ELISA reported in scientific literature (PMID 23973875)
Human, mouse, rat, monkey and Xenopus. Other species not tested.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
Protein G purified
Alternate Names for SMN Antibody (2B1)
survival of motor neuron 1, telomeric
Spinal muscular atrophy (SMA) is a common fatal autosomal recessive disorder characterized by the degeneration of motor neurons and muscular atrophy. A gene called Survival of Motor Neurons (SMN) has been identified as the determining gene in SMA and is deleted or mutated in greater than 98% of SMA patients. The SMN protein localizes to both the cytoplasm and the nucleus. In the nucleus, SMN has been found to localize in a nuclear structure termed gems for Gemini of coiled bodies, which contain high levels of small ribonucleoproteins (snRNPs). SMN forms complexes with proteins including SIP1, Gemin 2-7 and several others known to be involved in the biogenesis of snRNPs. SMN antibodies may serve as a useful research tool to study SMN complexes and gems, and their role in pre-RNA processing and RNA metabolism.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for SMN Antibody (NB100-1936). (Showing 1 - 1 of 1 FAQs).
Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.
We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.
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