Fibrillarin Antibody (38F3)

Images

 
Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - of whole yeast protein extracts with a collection of our antibodies. The blot for Fibrillarin Antibody (38F3) is in the indicated lane, and the number indicates ...read more
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - IF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used ...read more
Immunohistochemistry-Paraffin: Fibrillarin Antibody (38F3) [NB300-269] - IHC staining of Fibrillarin in human ovarian cancer using DAB with hematoxylin counterstain.
Flow Cytometry: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin antibody was tested at 1:400 in HEK293 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).
Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Western blot analysis of Fibrillarin expression on yeast protein extracts using NB300-269.
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Human SH-SY5Y cells stained with NB300-269, showing prominent specular nucleolar staining. The nuclei are counter stained with DAPI ...read more
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin immunofluorescence in fibroblasts. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ce, Ch, Dr, Pl, YeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
Clone
38F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Fibrillarin Antibody (38F3) Summary

Immunogen
Yeast nuclear preparation (S. cerevisiae).
Localization
Nucleus > nucleolus. Note: Fibrillar region of the nucleolus.
Marker
Nucleolar Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FBL
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Flow Cytometry 1:20-1:400
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunohistochemistry Whole-Mount
Application Notes
This Fibrillarin Antibody (38F3) is useful for Western blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Immunohistochemistry on both paraffin-embedded and frozen sections. In WB a band can be seen at approximately 34 kDa. In ICC/IF, this antibody shows prominent specular nucleolar staining. Use in Immunohistochemistry whole mount reported in scientific literature (PMID 24722283) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Fibrillarin Lysate (NBL1-10607)
Reviewed Applications
Read 2 Reviews rated 5
using
NB300-269 in the following applications:

Publications
Read Publications using
NB300-269 in the following applications:

Reactivity Notes

Human, rat, mouse, chicken, C. elegans, Drosophila, plants and yeast. Other species not tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative
10mM Sodium Azide
Purity
Affinity purified

Alternate Names for Fibrillarin Antibody (38F3)

  • EC 2.1.1,34-kD nucleolar scleroderma antigen
  • EC 2.1.1.-
  • EC 2.1.1.37
  • FIB
  • FIB1,34 kDa nucleolar scleroderma antigen
  • fibrillarin
  • FLRNrRNA 2'-O-methyltransferase fibrillarin
  • RNA, U3 small nucleolar interacting protein 1
  • RNU3IP1

Background

Fibrillarin / Nop1p, was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Ce, Ch, Dr, Pl, Ye
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt

Publications for Fibrillarin Antibody (NB300-269)(8)

We have publications tested in 5 confirmed species: Human, Mouse, C. elegans, Drosophila, PLANT.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, WB.


Filter By Application
ICC/IF
(3)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
IHC-WhMt
(1)
WB
(1)
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Filter By Species
Human
(3)
Mouse
(2)
C. elegans
(1)
Drosophila
(1)
PLANT
(1)
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Showing Publications 1 - 8 of 8.
Publications using NB300-269 Applications Species
Robaszkiewicz E, Idziak-Helmcke D, Tkacz MA et al. The arrangement of Brachypodium distachyon chromosomes in interphase nuclei. J. Exp. Bot. Oct 1 2016 [PMID: 27588463] (IHC, PLANT) IHC PLANT
Mitrea DM, Cika JA, Guy CS et al. Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA Elife Mar 1 2016 [PMID: 26836305] (ICC/IF, Mouse) ICC/IF Mouse
Itatani Y, Sonoshita M, Kakizaki F et al. Characterization of Aes nuclear foci in colorectal cancer cells. J. Biochem. 2015 Jul 29 [PMID: 26229111] (ICC/IF, Human)

Details:
Fibrillarin antibody was used for ICC/IF analysis of HCT116 cells overexpressing AcGFP-tagged Aes (AcGFP-Aes) and TLE1 (Fig. 1B).
ICC/IF Human
Zaballos MA, Cantero W, Azpiazu N. The TALE Transcription Factor Homothorax Functions to Assemble Heterochromatin during Drosophila Embryogenesis. PLoS ONE. 2015 Mar 21 [PMID: 25794008] (IHC, Drosophila) IHC Drosophila
Lee CC, Tsai YT, Kao CW et al. Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans. Cell Death Dis 2014 Apr 11 [PMID: 24722283] (IHC-WhMt, C. elegans) IHC-WhMt C. elegans
Zillner K, Filarsky M, Rachow K et al. Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 Nucleic Acids Res 2013 Apr 10 [PMID: 23580549] (ICC/IF, Human) ICC/IF Human
Su H, Xu T, Ganapathy S et al. Elevated snoRNA biogenesis is essential in breast cancer Oncogene 2013 Apr 1 [PMID: 23542174] (WB, IHC-P, Human) WB, IHC-P Human
Beyret E, Lin H. Pinpointing the expression of piRNAs and function of the PIWI protein subfamily during spermatogenesis in the mouse Dev Biol 2011 Jul 15 [PMID: 21539824] (IHC-Fr, Mouse) IHC-Fr Mouse

Reviews for Fibrillarin Antibody (NB300-269) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: ICC, IF.

Reviews using NB300-269:
Filter by Applications
ICC
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Fibrillarin NB300-269
Enlarge
reviewed by:
Anony- mous
ICC Human 06/09/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Immunofluorescence Fibrillarin NB300-269
Enlarge
reviewed by:
Jennifer Fox
IF Human 04/24/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedFibroblasts
SpeciesHuman
Lot92412

Blocking

Blocking Detailsblocked in 5% Normal Goat serum/0.3% Triton X-100 in PBS, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:500, 4C, overnight, in 1X PBS/1% BSA/0.3% Triton X-100

Secondary Antibody

Secondary Descriptiongoat anti mouse alexafluor 488 conjugated
Secondary Manufacturer Cat#cell signaling 4408
Secondary Concentration1:1000

Details

Fixation DetailsFixed in PFA, Blocked in Normal Goat serum containing Triton X-100, Incubated overnight 1:100 in 1% BSA
Wash Description1X PBS, 3 washes 5 minutes each

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibrillarin Antibody (NB300-269) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anony- mous
06/09/2014
Application: ICC
Species: Human

Jennifer Fox
04/24/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol FBL
Entrez

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