Fibrillarin Antibody (38F3)

Images

 
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media ...read more
Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Analysis of lysates of cell fractions probed with Fibrillarin antibody, dilution 1:500 (Green): [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 ...read more
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci and ectopic nucleoli and produce more rRNA. A) Wild type nuclei stained with ...read more
Immunohistochemistry-Paraffin: Fibrillarin Antibody (38F3) [NB300-269] - IHC staining of Fibrillarin in human ovarian cancer using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Mouse embryonic fibroblast fibrillarin stained with fibrillarin antibody and Alexa-594 secondary antibody. ICC/IF image submitted by a ...read more
Flow Cytometry: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin antibody was tested at 1:400 in HEK293 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).
Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Western blot analysis of Fibrillarin expression on yeast protein extracts using NB300-269.
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin immunofluorescence in fibroblasts. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - IF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ce, Ch, Dr, Eq, Pl, Y, YeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, KD
Clone
38F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Fibrillarin Antibody (38F3) Summary

Immunogen
Yeast nuclear preparation (S. cerevisiae).
Localization
Nucleus > nucleolus. Note: Fibrillar region of the nucleolus.
Marker
Nucleolar Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FBL
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20-1:400
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Knockdown Validated
  • Western Blot 1:1000-1:5000
Application Notes
This Fibrillarin Antibody (38F3) is useful for Western blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Immunohistochemistry on both paraffin-embedded and frozen sections. In WB a band can be seen at approximately 34.5 kDa. In ICC/IF, this antibody shows prominent specular nucleolar staining.

Use in IHC-WhMt and IHC-Fr reported in scientific literature (PMID: 24722283 and 21539824 respectively). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 23542174).
Theoretical MW
34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Fibrillarin Overexpression Lysate
Reviewed Applications
Read 4 Reviews rated 5
using
NB300-269 in the following applications:

Publications
Read Publications using
NB300-269 in the following applications:

Reactivity Notes

C. elegans and plant reactivity reported in scientific literature (PMID: 24722283 and 27588463 respectively). Use in S. pombe reported in scientific literature (PMID:31855181).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Supplied as concentrated hybridoma cell culture media.
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Fibrillarin Antibody (38F3)

  • EC 2.1.1,34-kD nucleolar scleroderma antigen
  • EC 2.1.1.-
  • EC 2.1.1.37
  • FIB
  • FIB1,34 kDa nucleolar scleroderma antigen
  • fibrillarin
  • FLRNrRNA 2'-O-methyltransferase fibrillarin
  • RNA, U3 small nucleolar interacting protein 1
  • RNU3IP1

Background

Fibrillarin / Nop1p, was originally identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-269
Species: Hu, Mu, Rt, Po, Bv, Ce, Ch, Dr, Eq, Pl, Y, Ye
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, KD

Publications for Fibrillarin Antibody (NB300-269)(20)

We have publications tested in 6 confirmed species: Human, Mouse, C. elegans, Drosophila, PLANT, S. pombe.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, WB.


Filter By Application
ICC/IF
(7)
IHC
(2)
IHC-Fr
(2)
IHC-P
(1)
IHC-WhMt
(1)
WB
(6)
All Applications
Filter By Species
Human
(5)
Mouse
(3)
C. elegans
(5)
Drosophila
(2)
PLANT
(1)
S. pombe
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB300-269 Applications Species
Hibino E, Ichiyama Y, Tsukamura A et al. Bex1 is essential for ciliogenesis and harbours biomolecular condensate-forming capacity BMC biology Feb 10 2022 [PMID: 35144600] (ICC/IF) ICC/IF
Chen P, Chen Y, Chu T et al. Nucleolar control by a non apoptotic p53 caspases deubiquitinylase axis promotes resistance to bacterial infection FASEB J Nov 29 2019 (WB, C. elegans) WB C. elegans
Wu T, Nance J, Chu F, Fazzio T Characterization of R-loop-interacting proteins in embryonic stem cells reveals roles in ribosomal RNA processing and gene expression Molecular & Cellular Proteomics Aug 1 2021 [PMID: 34478875]
Procida T, Friedrich T, Jack APM, et al. JAZF1, A Novel p400/TIP60/NuA4 Complex Member, Regulates H2A.Z Acetylation at Regulatory Regions International journal of molecular sciences Jan 12 2021 [PMID: 33445503] (ICC/IF, Human) ICC/IF Human
Sural S, Liang CY, Wang FY et al. HSB-1/HSF-1 pathway modulates histone H4 in mitochondria to control mtDNA transcription and longevity Sci Adv Oct 1 2020 [PMID: 33087356]
Begik O, Lucas MC, Liu H et al. Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signatures Genome Biol. May 7 2020 [PMID: 32375858] (IHC-Fr, Mouse) IHC-Fr Mouse
Hsiung K, Liu K, Tsai T et al. Defects in CISD-1, a mitochondrial iron-sulfur protein, lower glucose level and ATP production in Caenorhabditis elegans Biomedical Journal Feb 1 2020 [PMID: 32200954] (WB, C. elegans) WB C. elegans
Wong IN, Neo JP, Oehler J et al. The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast Elife Dec 19 2019 [PMID: 31855181] (WB, S. pombe) WB S. pombe
Tiku V, Kew C, Mehrotra P et al. Nucleolar fibrillarin is an evolutionarily conserved regulator of bacterial pathogen resistance. Nat Commun Sep 6 2018 [PMID: 30190478] (WB, C. elegans) WB C. elegans
Buchwalter A, Hetzer MW. Nucleolar expansion and elevated protein translation in premature aging Nat Commun 2017 Aug 30 [PMID: 28855503] (ICC/IF, Human) ICC/IF Human
Show All 20 Publications.

Reviews for Fibrillarin Antibody (NB300-269) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 4 species: Human, Mouse, C. elegans, Other.

Reviews using NB300-269:
Filter by Applications
Immunofluorescence
(1)
Western Blot
(2)
Immunocytochemistry
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
C. elegans
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Fibrillarin NB300-269
Enlarge
5
reviewed by:
Ahmed Mostafa
Immunofluorescence Mouse 06/14/2021
View

Summary

ApplicationImmunofluorescence
Sample Testedfibroblasts,Mouse embryonic fibroblasts
SpeciesMouse
Lot9A-1147725
  5
reviewed by:
Verified Customer
Western Blot C. elegans 01/09/2019
View

Summary

ApplicationWestern Blot
Sample Tested4% PFA-PBS fixed frozen sections from murine brain,Tissue Lysates
SpeciesC. elegans
Lot38F3
Immunocytochemistry Fibrillarin NB300-269
Enlarge
5
reviewed by:
Verified Customer
Immunocytochemistry Human 06/09/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
  5
reviewed by:
Jennifer Fox
Western Blot Other 04/24/2014
View

Summary

ApplicationWestern Blot
Sample Testedfibroblasts
SpeciesOther
Lot92412

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibrillarin Antibody (NB300-269) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Ahmed Mostafa
06/14/2021
Application: Immunofluorescence
Species: Mouse

 
Verified Customer
01/09/2019
Application: Western Blot
Species: C. elegans

Verified Customer
06/09/2014
Application: Immunocytochemistry
Species: Human

Bioinformatics

Gene Symbol FBL
Entrez