ZEB2 Antibody

Images

 
Orthogonal Strategies: Western Blot: ZEB2 Antibody [NBP1-82991] - Analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-ZEB2 antibody. Corresponding ZEB2 RNA-seq data are presented for the same cell lines. ...read more
Western Blot: ZEB2 Antibody [NBP1-82991] - Depletion of TRIM14 increased ZEB2 polyubiquitination and proteasomal degradation. Western blot analysis of TRIM14, ZEB2 and beta-actin in LN229 and U251 cells transduced with ...read more
Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human pancreas shows no positivity as expected.
Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.
Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human colon shows moderate nuclear positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: ZEB2 Antibody [NBP1-82991] - Staining of human glioma shows moderate nuclear positivity in tumor cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

ZEB2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SPVKPMDSITSPSIAELHNSVTNCDPPLRLTKPSHFTNIKPVEKLDHSRSNTPSPLNLSSTSSKNSHSSSYTPNSFSSEELQAEPLDLSLPKQMKEPKSIIATKNKTKASSISLDHNSVSSSSE
Predicted Species
Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZEB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
IHC, ICC/IF reported in scientific literature (PMID: 22965162). Flow reported in scientific literature (PMID: 28790065). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Knockdown validation (PMID: 31934283).
Control Peptide
ZEB2 Protein (NBP1-82991PEP)
Reviewed Applications
Read 2 Reviews rated 2.5
using
NBP1-82991 in the following applications:

Publications
Read Publications using
NBP1-82991 in the following applications:

  • 1 publication
  • 1 publication
  • IHC
    4 publications
  • 2 publications
  • KD
    1 publication
  • WB
    12 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22965162).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ZEB2 Antibody

  • HSPC082
  • KIAA0569FLJ42816
  • SIP1
  • SIP1HSPC082
  • Smad-interacting protein 1
  • SMADIP1
  • SMADIP1ZFHX1BSIP-1
  • ZEB2
  • ZFHX1B
  • ZFX1B
  • zinc finger E-box binding homeobox 2
  • zinc finger E-box-binding homeobox 2
  • zinc finger homeobox 1b
  • Zinc finger homeobox protein 1b

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-82991
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, KD

Publications for ZEB2 Antibody (NBP1-82991)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, IHC-P, KD, WB.


Filter By Application
Flow
(1)
ICC/IF
(1)
IHC
(4)
IHC-P
(2)
KD
(1)
WB
(12)
All Applications
Filter By Species
Human
(13)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NBP1-82991 Applications Species
Ichikawa MK, Endo K, Itoh Y et al. Ets family proteins regulate the EMT transcription factors Snail and ZEB in cancer cells FEBS open bio Apr 22 2022 [PMID: 35451213] (WB, Human) WB Human
Osada A, Endo K , Kimura Y et al. Addiction of mesenchymal phenotypes on the FGF/FGFR axis in oral squamous cell carcinoma cells BioRxiv (WB, Human) WB Human
Lohraseb I, McCarthy P, Secker G et al. Global ubiquitinome profiling identifies NEDD4 as a regulator of Profilin 1 and actin remodelling in neural crest cells Nature communications Apr 19 2022 [PMID: 35440627] (WB) WB
Wang J, Farkas C, Benyoucef A et al. Interplay between the EMT transcription factors ZEB1 and ZEB2 regulates hematopoietic stem and progenitor cell differentiation and hematopoietic lineage fidelity PLoS biology Sep 22 2021 [PMID: 34550965] (WB, Mouse) WB Mouse
Moro L, Simoneschi D, Kurz E et al. Epigenetic silencing of the ubiquitin ligase subunit FBXL7 impairs c-SRC degradation and promotes epithelial-to-mesenchymal transition and metastasis Nat. Cell Biol. Aug 24 2020 [PMID: 32839549]
Cai X, Feng S, Zhang J et al. USP18 deubiquitinates and stabilizes Twist1 to promote epithelial-mesenchymal transition in glioblastoma cells Am J Cancer Res Apr 1 2020 [PMID: 32368392] (WB, Human) WB Human
Xu R, Zhou F, Yu T et al. MicroRNA-940 inhibits epithelial-mesenchymal transition of glioma cells via targeting ZEB2 Am J Transl Res Dec 15 2019 [PMID: 31934283] (WB, KD, Human) WB, KD Human
Osada A H, Endo K et al. Addiction of mesenchymal phenotypes on the FGF/FGFR axis in oral squamous cell carcinoma cells. PLoS One May 11 2019 [PMID: 31682640] (WB, Human) WB Human
Wang P, Liu X, Han G et al. Downregulated lncRNA UCA1 acts as ceRNA to adsorb microRNA-498 to repress proliferation, invasion and epithelial mesenchymal transition of esophageal cancer cells by decreasing ZEB2 expression Cell Cycle Sep 1 2019 [PMID: 31387451] (WB, Human) WB Human
Ordonez, LD;Hay, T;McEwen, R;Polanska, UM;Hughes, A;Delpuech, O;Cadogan, E;Powell, S;Dry, J;Tornillo, G;Silcock, L;Leo, E;O Connor, MJ;Clarke, AR;Smalley, MJ; Rapid activation of epithelial-mesenchymal transition drives PARP inhibitor resistance in Brca2-mutant mammary tumours Oncotarget Apr 8 2019 [PMID: 31080552] (WB, Mouse) WB Mouse
Show All 22 Publications.

Reviews for ZEB2 Antibody (NBP1-82991) (2) 2.52

Average Rating: 2.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-82991:
Filter by Applications
Immunohistochemistry-Frozen
(1)
Western Blot
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen ZEB2 NBP1-82991
Enlarge
1
reviewed by:
Verified Customer
Immunohistochemistry-Frozen Mouse 11/08/2019
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFixed frozen mouse spinal cord
SpeciesMouse
LotB114435

Comments

CommentsNovus Innovators Program - new species or application used on a primary antibody.
Western Blot ZEB2 NBP1-82991
Enlarge
4
reviewed by:
Prabir Chakraborty
Western Blot Human 11/12/2017
View

Summary

ApplicationWestern Blot
Sample TestedCancer Cell lines
SpeciesHuman
LotB105866

Comments

CommentsSample: Whole cell lysates of various cancer cell lines (10ug)
Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature
Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C
Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZEB2 Antibody (NBP1-82991) (0)

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Secondary Antibodies

 

Isotype Controls

Additional ZEB2 Products

Array NBP1-82991

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Diseases for ZEB2 Antibody (NBP1-82991)

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Recent Reviews

2.5
5
0
4
1
3
0
2
0
1
1

Verified Customer
11/08/2019
Application: Immunohistochemistry-Frozen
Species: Mouse

Prabir Chakraborty
11/12/2017
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol ZEB2