ALS Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse spleen tissue, mouse liver tissue, and mouse bone marrow. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ALS Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

ALS Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALS
Thr28-Cys603
Accession # P70389
Specificity
Detects mouse ALS in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Igfals
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1436 in the following application:

Publications
Read Publication using
AF1436 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALS Antibody [Unconjugated]

  • ALS
  • Igfals

Background

ALS (Acid labile subunit) is an 84-86 kDa glycoprotein member of the leucine rich repeat superfamily of molecules.  It is secreted by hepatocytes in response to growth hormone stimulation, and forms a 140-150 kDa ternary complex with IGFBP3 (or IGFBP5) and IGF-I.  This complex increases the half-life of IGF-I and retains it in the circulation for use in multiple tissues.  Mature mouse ALS is 580 amino acids (aa) in length (aa 24-603).  It contains twenty-one 20-23 aa LRRs that participate in protein-protein interactions.  There are two potential isoform variants that show N-terminal extensions.  One contains a 66 aa substitution for aa 1-5, while another possesses an 89 aa substitution again for aa 1-5.  Over aa 28-603, mouse ALS shares 79% and 93% aa identity with human and rat ALS, respectively.        

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALS Antibody (AF1436)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Mouse
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Review for ALS Antibody (AF1436) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1436:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22999939
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22999939

Details

Detection NotesSee PMID 22999939

Comments

CommentsPublished. See PMID 22999939

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ALS Antibody (AF1436) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
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1
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1
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Anonymous
01/05/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol Igfals
Entrez
Uniprot