STRAP Antibody - BSA Free

Western Blot: STRAP Antibody [NBP2-20521] - Wild-type (WT) and STRAP knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: STRAP Antibody [NBP2-20521] - STRAP antibody [N1C3] detects STRAP protein by western blot analysis.A. 30 ug MCF-7 whole cell lysate/extractB. 30 ug MDA-MB-231 whole cell lysate/extract 10% SDS-PAGESTRAP antibody [N1C3] (NBP2-20521) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] (NBP2-20521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

STRAP antibody [N1C3] detects STRAP protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. STRAP antibody [N1C3] (NBP2-20521) dilution: 1:500. br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

STRAP antibody [N1C3] detects STRAP protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: STRAP protein stained by STRAP antibody [N1C3] (NBP2-20521) diluted at 1:500.Blue: Hoechst 33342 staining.

STRAP antibody [N1C3] detects STRAP protein by western blot analysis.A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract10% SDS-PAGESTRAP antibody [N1C3] (NBP2-20521) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] (NBP2-20521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

STRAP Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit STRAP Antibody - BSA Free (NBP2-20521) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human STRAP. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Bovine (99%), Chicken (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STRAP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (82%), Xenopus laevis (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for STRAP Antibody - BSA Free

• MAP activator with WD repeats
• MAWDpt-wd
• serine/threonine kinase receptor associated protein
• serine-threonine kinase receptor-associated protein
• UNR-interacting protein
• UNRIPPT-WD
• WD-40 repeat protein PT-WD

Background

The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. STRAP may play a role in the cellular distribution of the SMN complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for STRAP Antibody (NBP2-20521). (Showing 1 - 1 of 1 FAQ).

1. I am looking to buy an antibody for STRAP (serine-threonine receptor associated protein) and was wondering if you had published references related to the use of your STRAP antibodies. My application would be for Western blot.
   • Unfortunately, we do not have any published references related to the use of our STRAP antibodies yet. The antibodies were tested and validated and the images published on our website are in house images. If you would like me to help you choose an antibody that would be the best choice for your purposes please let me know what species you are working with. I also wanted to mention our 100% <a href="http://www.novusbio.com/support/quality-assurance.html" target="_self">Novus Guarantee</a> to you. We guarantee that the antibody you will use will work in species and application we have validated it in. If you experience any troubles, we will be happy to troubleshoot with you. However, should we fail to resolve your problems we will refund your purchase or replace the antibody with a more appropriate substitute.

Bioinformatics

• Gene Symbol: STRAP
• Uniprot:
  • Q9Y3F4(Human)