GTF2H2 Antibody (PCRP-GTF2H2-1B9)

Images

 
Immunocytochemistry/ Immunofluorescence: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Immunofluorescence Analysis of PFA-fixed MCF7 cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti- ...read more
Flow Cytometry: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti- Mouse IgG-CF488 (blue); ...read more
Immunocytochemistry/ Immunofluorescence: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Immunofluorescence Analysis of PFA-fixed HeLa cells. GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9) followed by goat anti- ...read more
Protein Array: GTF2H2 Antibody (PCRP-GTF2H2-1B9) [NBP3-07348] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2H2 Mouse Monoclonal Antibody (PCRP-GTF2H2-1B9).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, MA
Clone
PCRP-GTF2H2-1B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

GTF2H2 Antibody (PCRP-GTF2H2-1B9) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08257)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Recombinant full-length human GTF2H2 protein (Uniprot: Q13888)
Localization
Nucleus
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GTF2H2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for GTF2H2 Antibody (PCRP-GTF2H2-1B9)

  • Basic transcription factor 2 44 kDa subunit
  • BTF2 p44
  • BTF2
  • BTF2P44TFIIH basal transcription factor complex p44 subunit
  • General transcription factor IIH polypeptide 2
  • general transcription factor IIH subunit 2
  • general transcription factor IIH, polypeptide 2 (44kD subunit)
  • general transcription factor IIH, polypeptide 2, 44kD subunit
  • general transcription factor IIH, polypeptide 2, 44kDa
  • MGC102806
  • T-BTF2P44
  • TFIIH

Background

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GTF2H2
Uniprot