SMN Antibody (2B1) - Azide and BSA Free

Western Blot: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMN Antibody (2B1) NBP2-80955 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foc

Flow Cytometry: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - An intracellular stain was performed on Neuro2a cells with SMN Antibody (2B1) NBP2-80955 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against DyLight 488 (Green). Actin nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.

Immunohistochemistry: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - Analysis of SMN on mouse brain using NB100-1936. Image from the standard format of this antibody.

Flow Cytometry: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - An intracellular stain was performed on Ntera2 cells with SMN Antibody (2B1) NBP2-80955 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

• Reactivity: Hu, Mu, Rt, Pm, Xp, Ze
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Clone: 2B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

SMN Antibody (2B1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SMN Antibody (2B1) - Azide and BSA Free (NB100-1936) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant His6-tagged human SMN protein. [Swiss-Prot# Q16637]
• Localization: Cytoplasm. Nucleus > Gem, localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMN1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA reported in scientific literature (PMID 23973875)
  • Flow Cytometry reported in scientific literature (PMID 30102724)
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2 ug/ml
• Application Notes: This SMN Antibody (2B1) is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation, and Western blot where a band can be seen at ~ 35 kDa.

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 27273160).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SMN Antibody (2B1) - Azide and BSA Free

• Gemin-1
• Kugelberg-Welander disease)
• SMNC
• survival motor neuron protein
• survival of motor neuron 1, telomeric

Background

Spinal muscular atrophy (SMA) is a common fatal autosomal recessive disorder characterized by the degeneration of motor neurons and muscular atrophy. A gene called Survival of Motor Neurons (SMN) has been identified as the determining gene in SMA and is deleted or mutated in greater than 98% of SMA patients. The SMN protein localizes to both the cytoplasm and the nucleus. In the nucleus, SMN has been found to localize in a nuclear structure termed gems for Gemini of coiled bodies, which contain high levels of small ribonucleoproteins (snRNPs). SMN forms complexes with proteins including SIP1, Gemin 2-7 and several others known to be involved in the biogenesis of snRNPs. SMN antibodies may serve as a useful research tool to study SMN complexes and gems, and their role in pre-RNA processing and RNA metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SMN Antibody (NBP2-80955). (Showing 1 - 1 of 1 FAQ).

1. Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.
   • We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

Bioinformatics

• Gene Symbol: SMN1