SMN Antibody (2B1) - Azide and BSA Free


Western Blot: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates. Image from the standard more
Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against DyLight 488 (Green). Actin nuclei were counterstained more
Immunohistochemistry: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - Analysis of SMN on mouse brain using NB100-1936. Image from the standard format of this antibody.
Immunohistochemistry: SMN Antibody (2B1) - Azide and BSA Free [NBP2-80955] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. more

Product Details

Reactivity Hu, Mu, Rt, Pm, Xp, ZeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Azide and BSA Free
1 mg/ml

Order Details

SMN Antibody (2B1) - Azide and BSA Free Summary

Purified recombinant His6-tagged human SMN protein. [Swiss-Prot# Q16637]
Cytoplasm. Nucleus > Gem, localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).
Zebrafish reactivity reported in scientific literature (PMID: 27273160).
Protein G purified
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  • Western Blot : 2 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence : 1:250
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin : 1:200
  • Immunoprecipitation : 1:10-1:500
Application Notes
This SMN Antibody (2B1) is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry, Immunoprecipitation, and Western blot where a band can be seen at ~ 35 kDa. Use in ELISA reported in scientific literature (PMID: 23973875) Use in FLOW reported in scientific literature (PMID: 30102724).

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 27273160).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine, 0.15 M NaCl
No Preservative
1 mg/ml
Protein G purified

Alternate Names for SMN Antibody (2B1) - Azide and BSA Free

  • Gemin-1
  • Kugelberg-Welander disease)
  • SMNC
  • survival motor neuron protein
  • survival of motor neuron 1, telomeric


Spinal muscular atrophy (SMA) is a common fatal autosomal recessive disorder characterized by the degeneration of motor neurons and muscular atrophy. A gene called Survival of Motor Neurons (SMN) has been identified as the determining gene in SMA and is deleted or mutated in greater than 98% of SMA patients. The SMN protein localizes to both the cytoplasm and the nucleus. In the nucleus, SMN has been found to localize in a nuclear structure termed gems for Gemini of coiled bodies, which contain high levels of small ribonucleoproteins (snRNPs). SMN forms complexes with proteins including SIP1, Gemin 2-7 and several others known to be involved in the biogenesis of snRNPs. SMN antibodies may serve as a useful research tool to study SMN complexes and gems, and their role in pre-RNA processing and RNA metabolism.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMN Antibody (NBP2-80955). (Showing 1 - 1 of 1 FAQ).

  1. Could you recommond the Human specific SMN antibody? It will only recognize human SMN, without cross reaction with mouse smn.
    • We do not have an SMN antibody that has been validated NOT to work in mouse samples. When an antibody is found to not react with a certain species, we mark the data sheet with a (-) next to the species' name. Unfortunately, the SMN antibodies that do not list mouse have not been tested in mouse, and may react.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-1936AF350
Alexa Fluor 405 NB100-1936AF405
Alexa Fluor 488 NB100-1936AF488
Alexa Fluor 532 NB100-1936AF532
Alexa Fluor 594 NB100-1936AF594
Alexa Fluor 647 NB100-1936AF647
Alexa Fluor 700 NB100-1936AF700
Alexa Fluor 750 NB100-1936AF750
Biotin NB100-1936B
DyLight 350 NB100-1936UV
DyLight 405 NB100-1936V
DyLight 488 NB100-1936G
DyLight 550 NB100-1936R
DyLight 594 NB100-1936DL594
DyLight 650 NB100-1936C
DyLight 680 NB100-1936FR
DyLight 755 NB100-1936IR
FITC NB100-1936F
HRP NB100-1936H
Janelia Fluor 549 NB100-1936JF549
Janelia Fluor 646 NB100-1936JF646

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Gene Symbol SMN1