NAIP Antibody - BSA Free

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Biological Strategies: Immunocytochemistry/ Immunofluorescence: NAIP Antibody [NBP1-77196] - Confocal microscopy analysis of NAIP expression in THP-1m with or without CFT073 stimulation. NLRC4 and NAIP expression ...read more
Western Blot: NAIP Antibody [NBP1-77196] - Analysis of NLRP3, NLRC4, NAIP, AIM2, ASC, Caspase-4, Caspase-1, B-actin and GAPDH protein expression by immunoblotting. (A) Represents the immunoblotting results of NLRP3, ...read more
Western Blot: NAIP Antibody [NBP1-77196] - Analysis of NAIP in human tonsil tissue lysate with NAIP antibody at 1 ug/mL.
Immunocytochemistry: NAIP Antibody [NBP1-77196] - A549 cells with NAIP antibody at 10 ug/ml.
Immunofluorescence: NAIP Antibody [NBP1-77196] - Immunofluorescence of NAIP in A549 cells with NAIP antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Ec, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, PAGE, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

NAIP Antibody - BSA Free Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human NAIP. The immunogen is located within amino acids 1200 - 1250 of NAIP. Amino Acid Squence: QFPDEETSEKFAY
Specificity
Minor, lower molecular weight bands may represent alternately spliced forms.
Predicted Species
Mouse (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAIP
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/mL
  • Immunofluorescence 20 ug/ml
  • Immunomicroscopy
  • Immunoprecipitation
  • SDS-Page
  • Western Blot 0.5-1 ug/ml
Application Notes
Use in SDS- Page , Immunoprecipitation and Immunomicroscopy reported in scientific literature (PMID:31551961).
Theoretical MW
155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
NAIP Peptide (NBP1-77196PEP)
Publications
Read Publication using
NBP1-77196 in the following applications:

  • IM
    1 publication
  • IP
    1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

E. coli reactivity reported in scientific literature (PMID: 31551961).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for NAIP Antibody - BSA Free

  • baculoviral IAP repeat-containing 1
  • BIRC1
  • BIRC1baculoviral IAP repeat-containing protein 1
  • FLJ18088
  • FLJ42520
  • FLJ58811
  • NAIP
  • Neuronal apoptosis inhibitory protein
  • NLR family, apoptosis inhibitory protein
  • NLR family, BIR domain containing 1
  • NLRB1
  • nucleotide-binding oligomerization domain, leucine rich repeat and BIR domaincontaining 1
  • psi neuronal apoptosis inhibitory protein
  • psiNAIP

Background

Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR); these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules (reviewed in 3). Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NAIP Antibody (NBP1-77196)(1)

We have publications tested in 1 confirmed species: E. coli.

We have publications tested in 4 applications: IM, IP, PAGE, WB.


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IM
(1)
IP
(1)
PAGE
(1)
WB
(1)
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Filter By Species
E. coli
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NAIP Antibody (NBP1-77196) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Research Areas for NAIP Antibody (NBP1-77196)

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Blogs on NAIP.


  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

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Bioinformatics

Gene Symbol NAIP
Uniprot