hnRNP A1 Antibody (4B10) - BSA Free

Images

 
Genetic Strategies: Knockdown Validated: hnRNP A1 Antibody (4B10) [NB100-672] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and hnRNP A1 knockout (KO) HEK293T cell line. PVDF ...read more
Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Whole cell protein from HeLa (lane 1), A431 (lane 2), Hek293 (lane 3) and MCF7 (lane 4) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP A1 Antibody (4B10) [NB100-672] - The hnRNP A1 (4B10) antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Actin and nuclei were counterstained ...read more
Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-hnRNP-A1 Clone: 4B10 (NB100-672). Lanes: 1-HeLa, 2-Jurkat, 3-HEK-293T, 4-WiDr, ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, MiAr, KD
Clone
4B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

hnRNP A1 Antibody (4B10) - BSA Free Summary

Immunogen
Full length partially purified human hnRNP A1. [UniProt# P09651]
Localization
Nucleus. Cytoplasm. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes.
Specificity
Does not react with hnRNP A2 or B
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HNRNPA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Indirect
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Microarray
  • Western Blot 0.25 - 0.5 ug/ml
Application Notes
In WB a band can be seen between 32-35 kDa. This antibody immunoprecipitates the hnRNP complex. Use in Immunohistochemistry reported in scientific literature (PMID: 3129338).
Control
A-431 Whole Cell Lysate
HeLa Whole Cell Lysate
HEK293 Cell Lysate
MCF-7 Cell Lysate
Publications
Read Publications using
NB100-672 in the following applications:

  • 1 publication
  • 8 publications
  • IP
    6 publications
  • WB
    12 publications

Reactivity Notes

Human, bovine, canine and mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for hnRNP A1 Antibody (4B10) - BSA Free

  • Helix-destabilizing protein
  • heterogeneous nuclear ribonucleoprotein A1
  • heterogeneous nuclear ribonucleoprotein A1B protein
  • heterogeneous nuclear ribonucleoprotein B2 protein
  • heterogeneous nuclear ribonucleoprotein core protein A1
  • hnRNP A1
  • hnRNP core protein A1
  • hnRNPA1
  • hnRNP-A1
  • HNRPA1MGC102835
  • nuclear ribonucleoprotein particle A1 protein
  • single-strand DNA-binding protein UP1
  • Single-strand RNA-binding protein

Background

RNA polymerase II trans cripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, premRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts.2, 4, 5 Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa.2, 4, 5 hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein binds to its RNA target through a consensus RNA-binding site UAGGGU. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP -A1 from transportin. hnRNP-A1 is ubiquitously expressed with a higher expression in proliferating and/or transformed cells than in differentiated tissues.6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for hnRNP A1 Antibody (NB100-672)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA, ICC/IF, IP, WB.


Filter By Application
ELISA
(1)
ICC/IF
(8)
IP
(6)
WB
(12)
All Applications
Filter By Species
Human
(14)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NB100-672 Applications Species
Fang MY, Markmiller S, Vu AQ, et al. Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD Neuron 2019 Jun 18 [PMID: 31272829]
Marrone, L;Drexler, HCA;Wang, J;Tripathi, P;Distler, T;Heisterkamp, P;Anderson, EN;Kour, S;Moraiti, A;Maharana, S;Bhatnagar, R;Belgard, TG;Tripathy, V;Kalmbach, N;Hosseinzadeh, Z;Crippa, V;Abo-Rady, M;Wegner, F;Poletti, A;Troost, D;Aronica, E;Busskamp, V; FUS pathology in ALS is linked to alterations in multiple ALS-associated proteins and rescued by drugs stimulating autophagy Acta Neuropathol. Apr 1 2019 [PMID: 30937520] (ICC/IF, Human) ICC/IF Human
Gasperini L, Rossi A, Cornella N et al. The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization. Mol. Biol. Cell. Oct 24 2018 [PMID: 30354839] (WB, Human) WB Human
Lampe S, Kunze M, Scholz A, Brau TF. Identification of the TXNIP IRES and characterization of the impact of regulatory IRES trans-acting factors. Biochim. Biophys. Acta. 2018 Feb 01 [PMID: 29378331] (WB) WB
Hu J, Khodadadi-Jamayran A, Mao M et al. AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors. Nat Commun. Nov 8 2016 [PMID: 27824034]
Kamelgarn M, Chen J, Kuang L et al. Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS Biochim. Biophys. Acta 2016 Jul 25 [PMID: 27460707] (WB) WB
Kunze MM, Benz F, Braub TF et al. sST2 translation is regulated by FGF2 via an hnRNP A1-mediated IRES-dependent mechanism. Biochim. Biophys. Acta. May 8 2016 [PMID: 27168114] (ICC/IF, WB, IP, Human) ICC/IF, WB, IP Human
Fujiya M, Konishi H, Mohamed Kamel MK et al. microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1. Oncogene. 2013 Oct 28 [PMID: 24166503] (WB, Human) WB Human
Huelga SC, Vu AQ, Arnold JD et al. Integrative genome-wide analysis reveals cooperative regulation of alternative splicing by hnRNP proteins Cell Rep 2012 Feb [PMID: 22574288] (Human) Human
Lin, Y-T, Yen, P H. A novel nucleocytoplasmic shuttling sequence of DAZAP1, a testis-abundant RNA-binding protein, . 12, 1486-1493. 2006 [PMID: 16772659]
Show All 21 Publications.

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hnRNP A1 - a ribonucleoprotein regulating gene expression at many levels
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is an abundant ubiquitously expressed protein with important roles in the regulation of gene expression. hnRNP A1 is involved in transcription as well as the splicing, trafficking, and translati...  Read full blog post.

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Bioinformatics

Gene Symbol HNRNPA1
Entrez
Uniprot