hnRNP A1 Antibody (4B10)

Images

 
Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Whole cell protein from HeLa (lane 1), A431 (lane 2), Hek293 (lane 3) and MCF7 (lane 4) were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP A1 Antibody (4B10) [NB100-672] - The hnRNP A1 (4B10) antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Actin and nuclei were counterstained ...read more
Western Blot: hnRNP A1 Antibody (4B10) [NB100-672] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-hnRNP-A1 Clone: 4B10 (NB100-672). Lanes: 1-HeLa, 2-Jurkat, 3-HEK-293T, 4-WiDr, ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IP, MiAr
Clone
4B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

hnRNP A1 Antibody (4B10) Summary

Immunogen
Full length partially purified human hnRNP A1. [UniProt# P09651]
Localization
Nucleus. Cytoplasm. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes.
Specificity
Does not react with hnRNP A2 or B
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HNRNPA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 - 0.5 ug/ml
  • ELISA Indirect
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Microarray
Application Notes
In WB a band can be seen between 32-35 kDa. This antibody immunoprecipitates the hnRNP complex.
Positive Control
A-431 Lysate (NB800-PC12)
HeLa Lysate (NB800-PC1)
HEK293 Lysate (NBP2-25048)
MCF-7 Lysate (NBP2-25053)
Publications
Read Publications using
NB100-672 in the following applications:

  • 1 publication
  • 7 publications
  • IP
    6 publications
  • WB
    10 publications

Reactivity Notes

Human, bovine, canine and mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for hnRNP A1 Antibody (4B10)

  • Helix-destabilizing protein
  • heterogeneous nuclear ribonucleoprotein A1
  • heterogeneous nuclear ribonucleoprotein A1B protein
  • heterogeneous nuclear ribonucleoprotein B2 protein
  • heterogeneous nuclear ribonucleoprotein core protein A1
  • hnRNP A1
  • hnRNP core protein A1
  • hnRNPA1
  • hnRNP-A1
  • HNRPA1MGC102835
  • nuclear ribonucleoprotein particle A1 protein
  • single-strand DNA-binding protein UP1
  • Single-strand RNA-binding protein

Background

RNA polymerase II trans cripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, premRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts.2, 4, 5 Isolated hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. The major steady-state proteins of the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range of molecular weight starting with 34 kDa up to 43 kDa.2, 4, 5 hnRNP-A1 is important in pre-mRNA processing and in mRNA export from the nucleus. The protein binds to its RNA target through a consensus RNA-binding site UAGGGU. The protein contains a 38-amino acid domain called M9, which is important for the interaction with the transportin protein and therefore, for its import and export from the nucleus. RanGTP mediates dissociation of hnRNP -A1 from transportin. hnRNP-A1 is ubiquitously expressed with a higher expression in proliferating and/or transformed cells than in differentiated tissues.6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for hnRNP A1 Antibody (NB100-672)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA, ICC/IF, IP, WB.


Filter By Application
ELISA
(1)
ICC/IF
(7)
IP
(6)
WB
(10)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB100-672 Applications Species
Hu J, Khodadadi-Jamayran A, Mao M et al. AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors. Nat Commun. Nov 8 2016 [PMID: 27824034]
Kamelgarn M, Chen J, Kuang L et al. Proteomic analysis of FUS interacting proteins provides insights into FUS function and its role in ALS Biochim. Biophys. Acta 2016 Jul 25 [PMID: 27460707] (WB) WB
Kunze MM, Benz F, Braub TF et al. sST2 translation is regulated by FGF2 via an hnRNP A1-mediated IRES-dependent mechanism. Biochim. Biophys. Acta. May 8 2016 [PMID: 27168114] (ICC/IF, WB, IP, Human) ICC/IF, WB, IP Human
Fujiya M, Konishi H, Mohamed Kamel MK et al. microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1. Oncogene. 2013 Oct 28 [PMID: 24166503] (WB, Human) WB Human
Huelga SC, Vu AQ, Arnold JD et al. Integrative genome-wide analysis reveals cooperative regulation of alternative splicing by hnRNP proteins Cell Rep 2012 Feb [PMID: 22574288] (Human) Human
Lin, Y-T, Yen, P H. A novel nucleocytoplasmic shuttling sequence of DAZAP1, a testis-abundant RNA-binding protein,. 12, 1486-1493. 2006 [PMID: 16772659]
Bonnal S, Pileur F, Orsini C et al. Heterogeneous nuclear ribonucleoprotein A1 is a novel internal ribosome entry site trans-acting factor that modulates alternative initiation of translation of the fibroblast growth factor 2 mRNA. J Biol Chem. 2005 Feb 11 [PMID: 15525641] (WB, IP, Human) WB, IP Human
Pino I, Pio R, Toledo G et al. Altered patterns of expression of members of the heterogeneous nuclear ribonucleoprotein (hnRNP) family in lung cancer. Lung Cancer. 2003 Aug [PMID: 12871776] (ICC/IF, WB, Human) ICC/IF, WB Human
Guil S, Gattoni R, Carrascal M et al. Roles of hnRNP A1, SR proteins, and p68 helicase in c-H-ras alternative splicing regulation. Mol Cell Biol. 2003 Apr [PMID: 12665590] (WB, Human) WB Human
Mili S, Shu HJ, Zhao Y et al. Distinct RNP complexes of shuttling hnRNP proteins with pre-mRNA and mRNA: candidate intermediates in formation and export of mRNA. Mol Cell Biol. 2001 Nov [PMID: 11585913] (WB, Human) WB Human
Show All 16 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-672AF405
Alexa Fluor 488 Labeled NB100-672AF488
Alexa Fluor 647 Labeled NB100-672AF647
Alexa Fluor 700 Labeled NB100-672AF700
DyLight 350 Labeled NB100-672UV
DyLight 405 Labeled NB100-672V
DyLight 488 Labeled NB100-672G
DyLight 680 Labeled NB100-672FR
DyLight 755 Labeled NB100-672IR

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Blogs on hnRNP A1.

hnRNP A1 - a ribonucleoprotein regulating gene expression at many levels
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is an abundant ubiquitously expressed protein with important roles in the regulation of gene expression. hnRNP A1 is involved in transcription as well as the splicing, trafficking, and translati...  Read full blog post.

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Bioinformatics

Gene Symbol HNRNPA1
Entrez
Uniprot