SCAF11/SFRS2IP Antibody

Images

 
Western Blot: SCAF11/SFRS2IP Antibody [NB100-55266] - Detection of Human SFRS2IP on HeLa whole cell lysate using NB100-55266. SFRS2IP was also immunoprecipitated by another rabbit anti-SFRS2IP antibody.
Immunohistochemistry: SCAF11/SFRS2IP Antibody [NB100-55266] - Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti- SFRS2IP used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: SCAF11/SFRS2IP Antibody [NB100-55266] - FFPE section of human seminoma. Affinity purified rabbit anti-SFRS2IP used at a dilution of 1:100. Detection: DAB staining using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

SCAF11/SFRS2IP Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1098 and the C-terminus (residue 1148) of human splicing factor, arginine/serine-rich 2, interacting protein using the numbering given in entry CAA72121.1 (GeneID 9169).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCAF11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100 to 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
Application Notes
This antibody is useful for Western Blot, Immunohistochemistry-Paraffin and immunoprecipitation Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. ICC from customer review.
Reviewed Applications
Read 1 Review rated 3
using
NB100-55266 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Notes

Not to be confused with rodent Caspase 11, which is synonymouse with Caspase 4. Human Caspase 11 alternate names: anti-arginine/serine-rich 2 antibody, anti-CASP11 antibody, anti-Caspase 11 apoptosis related cysteine protease, anti-caspase 4 antibody, anti-Caspase 4 apoptosis related cysteine peptidas, anti-Caspase11 antibody, Anti-Caspase4 antibody, anti-CASPL antibody, anti-CTD-associated SR protein 11 antibody, anti-ICH 3 antibody, anti-ICH3 antibody, anti-ICH3 protease antibody, anti-interacting protein antibody, anti-SC35-interacting protein 1 antibody, anti-SFRS2IP antibody, anti-SIP1 antibody, anti-splicing factor antibody, anti-Splicing regulatory protein 129 antibody, anti-SRrp129 antibody

Alternate Names for SCAF11/SFRS2IP Antibody

  • CASP11Splicing regulatory protein 129
  • Renal carcinoma antigen NY-REN-40
  • serine/arginine-rich splicing factor 2, interacting protein
  • Serine/arginine-rich splicing factor 2-interacting protein
  • SFRS2-interacting protein
  • SFRS2IPSC35-interacting protein 1
  • SIP1SRSF2IP
  • splicing factor, arginine/serine-rich 2, interacting protein
  • Splicing factor, arginine/serine-rich 2-interacting protein
  • SR-related and CTD-associated factor 11
  • SR-related CTD-associated factor 11
  • SRrp129
  • SRRP129CTD-associated SR protein 11
  • SRSF2-interacting protein

Background

SFRS21P plays a role in pre-mRNA alternative splicing by regulating spliceosome assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-82991
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-05987
Species: Hu, Mu, Rt
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
NBP1-83976
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-37578
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NBP1-77177
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, ICC, IF
NBP2-03886
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
NBP1-82075
Species: Hu
Applications: WB, IHC, IHC-P
H00006607-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-56565
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NB100-1936
Species: Hu, Mu, Rt, Pm, Xp
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-20439
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-76798
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-37364
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC
NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-52453
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC, IF

Publications for SCAF11/SFRS2IP Antibody (NB100-55266) (0)

There are no publications for SCAF11/SFRS2IP Antibody (NB100-55266).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for SCAF11/SFRS2IP Antibody (NB100-55266) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB100-55266:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence SCAF11/SFRS2IP NB100-55266
Enlarge
reviewed by:
Simona Darovic
IF Human 01/29/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
LotA-1
CommentsWe have also teseted permeabilisation with MeOH and it worked. We also used antibody for Western blot and it worked, but I think that antibody is losing its activity, because we started with dilution 1:2000 and it worked perfectly. Now we have to use dilution 1:500 to detect band on Westren blot.

Blocking

Blocking Details3% BSA in PBS

Primary Anitbody

Dilution RatioantiCapase11 NB100-55266 1:100 in 3% BSA in PBS; 1 hour at room temperature

Secondary Antibody

Secondary DescriptionAlexaFluor 555 goat anti-rabbit (H+L)
Secondary Manufacturer Cat#Life technologies; A21428
Secondary Concentration1:1000

Details

Detection NotesZeiss AxioObserver; exposure time: 0,7s
Fixation DetailsFixation: 4%PFA in PBS 15 min; Permeabilization: 0,1% Triton X-100 in PBS 6 min
Wash Description3x5min vith PBS

Comments

CommentsWe have also teseted permeabilisation with MeOH and it worked. We also used antibody for Western blot and it worked, but I think that antibody is losing its activity, because we started with dilution 1:2000 and it worked perfectly. Now we have to use dilution 1:500 to detect band on Westren blot.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SCAF11/SFRS2IP Antibody (NB100-55266) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SCAF11/SFRS2IP Products

Bioinformatics Tool for SCAF11/SFRS2IP Antibody (NB100-55266)

Discover related pathways, diseases and genes to SCAF11/SFRS2IP Antibody (NB100-55266). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Research Areas for SCAF11/SFRS2IP Antibody (NB100-55266)

Find related products by research area.

Blogs on SCAF11/SFRS2IP

There are no specific blogs for SCAF11/SFRS2IP, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

Simona Darovic
01/29/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol SCAF11
Entrez
Uniprot