SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free

Staining of human adrenal gland shows moderate nuclear positivity in glandular cells.

Staining of human breast shows weak to moderate nuclear positivity in glandular cells.

Staining of human ovary shows moderate positivity in stromal cells.

Staining of human liver shows no positivity in hepatocytes as expected.

Analysis in human cell line HEL.

Staining of human testis shows weak to moderate nuclear positivity in Leydig cells and cells in seminiferous ducts.

Staining of U-2 OS cells using the Anti-SF-1/NR5A1/Steroidogenic Factor 1 monoclonal antibody) .

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL9101
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of Q13285, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR5A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
• Application Notes: For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) - Azide and BSA Free

• AD4BP
• AD4BPSTF-1
• Adrenal 4-binding protein
• ELP
• FTZ1adrenal 4 binding protein
• FTZF1
• FTZF1nuclear receptor AdBP4
• Fushi tarazu factor homolog 1
• NR5A1
• Nuclear receptor subfamily 5 group A member 1
• nuclear receptor subfamily 5, group A, member 1
• SF1
• SF-1
• SF-1POF7
• SF1steroidogenic factor 1
• Steroid hormone receptor Ad4BP
• steroidogenic factor-1

Background

Steroidogenic factor 1 (SF-1), a NR5 Fushi Tarazu-Like Receptor, has been shown to affect sexual differentiation, steroid metabolism, and neural development. SF-1 binds as a monomer and regulates all steroidogenic p450 genes, Mullerian inhibiting substance, the gonadotrophins, the ACTH receptor, oxytocin, nuclear receptor DAX-1, and several other genes. Four alternatively spliced isoforms of SF-1 have been isolated in mice, and they differ at the N and C termini but are identical in the DNA-binding domain. SF-1 expression has been documented in human brain, adrenal, spleen, liver, heart, ovary, testis, and placenta. ESTs have been isolated from human tissue libraries, including cancerous adrenal and normal lung and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR5A1