Cytochrome P450 17A1 Antibody (OTI3F11)

Images

 
Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.
Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Jurkat cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human breast tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human tonsil using anti-Cytochrome P450 17A1 mouse monoclonal antibody.
Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in 13 year old testis. Immunoexpression in Leydig cell. Image from a verified customer review.
Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Hela cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
OTI3F11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Cytochrome P450 17A1 Antibody (OTI3F11) Summary

Immunogen
Full length human recombinant protein of human CYP17A1(NP_000093) peoduced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYP17A1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Application Notes
IHC reported in a verified customer review.
Theoretical MW
57.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-01151 in the following applications:

Publications
Read Publication using
NBP2-01151 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Cytochrome P450 17A1 Antibody (OTI3F11)

  • CPT7
  • cyp17
  • CYP17A1
  • cytochrome p450 XVIIA1
  • cytochrome P450, family 17, subfamily A, polypeptide 1
  • cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenalhyperplasia
  • Cytochrome P450c17
  • Cytochrome P450-C17
  • EC 1.14.99
  • P450C17
  • P450C17CYPXVII
  • S17AH

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytochrome P450 17A1 Antibody (NBP2-01151)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
Human
(1)
All Species

Reviews for Cytochrome P450 17A1 Antibody (NBP2-01151) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rhesus macaque.

Reviews using NBP2-01151:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Human
(1)
Rhesus macaque
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Cytochrome P450 17A1 NBP2-01151
Enlarge
reviewed by:
Paula Aliberti
IHC-P Rhesus macaque 11/24/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPubertal testis
SpeciesRhesus macaque
LotA003
Immunohistochemistry-Paraffin Cytochrome P450 17A1 NBP2-01151
Enlarge
reviewed by:
Paula Aliberti
IHC-P Human 10/09/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman testis
SpeciesHuman
LotA003

Comments

Commentsprimary abtibody diluted 1/250

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Cytochrome P450 17A1 Antibody (NBP2-01151) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70544AF350
Alexa Fluor 405 NBP2-70544AF405
Alexa Fluor 488 NBP2-70544AF488
Alexa Fluor 532 NBP2-70544AF532
Alexa Fluor 594 NBP2-70544AF594
Alexa Fluor 647 NBP2-70544AF647
Alexa Fluor 700 NBP2-70544AF700
Alexa Fluor 750 NBP2-70544AF750
Allophycocyanin NBP2-70544APC
Biotin NBP2-70544B
DyLight 350 NBP2-70544UV
DyLight 405 NBP2-70544V
DyLight 488 NBP2-70544G
DyLight 550 NBP2-70544R
DyLight 594 NBP2-70544DL594
DyLight 650 NBP2-70544C
DyLight 680 NBP2-70544FR
DyLight 755 NBP2-70544IR
FITC NBP2-70544F
HRP NBP2-70544H
Janelia Fluor 549 NBP2-70544JF549
Janelia Fluor 646 NBP2-70544JF646
PE NBP2-70544PE
PerCP NBP2-70544PCP

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Bioinformatics Tool for Cytochrome P450 17A1 Antibody (NBP2-01151)

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Paula Aliberti
11/24/2017
Application: IHC-P
Species: Rhesus macaque

Paula Aliberti
10/09/2017
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CYP17A1
Entrez
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