Cytochrome P450 17A1 Antibody (OTI3F11)

Western Blot: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 17A1.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.

Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Jurkat cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human breast tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human Ovary tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Staining of paraffin-embedded Human tonsil using anti-Cytochrome P450 17A1 mouse monoclonal antibody.

Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in 13 year old testis. Immunoexpression in Leydig cell. Image from a verified customer review.

Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Hela cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: OTI3F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Cytochrome P450 17A1 Antibody (OTI3F11) Summary

• Immunogen: Full length human recombinant protein of human CYP17A1(NP_000093) peoduced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYP17A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 57.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cytochrome P450 17A1 Antibody (OTI3F11)

• CPT7
• CYP17
• CYP17A1
• CYPXVII
• Cytochrome P450 17A1
• cytochrome p450 XVIIA1
• cytochrome P450, family 17, subfamily A, polypeptide 1
• cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenalhyperplasia
• Cytochrome P450c17
• Cytochrome P450-C17
• EC 1.14.14.19
• EC 1.14.14.32
• EC 1.14.99
• P450C17
• P450C17CYPXVII
• S17AH

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytochrome P450 17A1 Antibody (NBP2-01151)(6)

Publications using NBP2-01151

• Baskin L, Cao M, Askel S et al. Ovotesticular cords and ovotesticular follicles: New markers in a model of human mixed ovotestis Differentiation; research in biological diversity 2023-11-19 [PMID: 38000942] (IHC, Human)
• Overland M, Li Y, Derpinghaus A et al. Development of the human ovary: Fetal through pubertal ovarian morphology, folliculogenesis and expression of cellular differentiation markers Differentiation 2022-10-01 [PMID: 36347737] (IF/IHC, Human)
• Cunha G, Cao M, Aksel S et al. Mouse-human species differences in early testicular development and its implications Differentiation 2022-04-01 [PMID: 35667976]
• Li Y, Overland M, Derpinghaus A et al. Development of the human fetal testis: Morphology and expression of cellular differentiation markers Differentiation 2022-03-01 [PMID: 35490077] (IF/IHC, Human)
• Grzesiak M, Kapusta K, Kaminska K et al. Effect of dietary supplementation with nettle or fenugreek on folliculogenesis and steroidogenesis in the rabbit ovary - An in vivo study Theriogenology 2021-06-08 [PMID: 34126406]
• Spade DJ, Hall SJ, Saffarini C et al. Differential response to abiraterone acetate and di-n-butyl phthalate in an androgen-sensitive human fetal testis xenograft bioassay. Toxicol Sci. 2013-11-27 [PMID: 24284787] (IHC-P, Human)

Reviews for Cytochrome P450 17A1 Antibody (NBP2-01151) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Rhesus Macaque.

reviewed by: Paula Aliberti IHC-P Rhesus Macaque 11/24/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Pubertal testis
• Species: Rhesus Macaque
• Lot: A003

reviewed by: Paula Aliberti IHC-P Human 10/09/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human testis
• Species: Human
• Lot: A003

Comments

• Comments: primary abtibody diluted 1/250

Bioinformatics

• Gene Symbol: CYP17A1
• Entrez:
  • 1586(Human)