SOX9 Antibody [Unconjugated]

Images

 
SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) ...read more
SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO‑III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular ...read more
Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for SOX9 at approximately 107 kDa (as indicated) using 20 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

SOX9 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX9
Met1-Lys151
Accession # P48436
Specificity
Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) SOX10 is observed, and less than 15% rhSOX11, rhSOX12, and rhSOX15 is observed, and less than 5% cross-reactivity with rhSOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX8, rhSOX13, rhSOX17, rhSOX18, rhSOX21, and rhSOX30 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX9
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 20 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF3075 in the following applications:

Publications
Read Publications using
AF3075 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX9 Antibody [Unconjugated]

  • campomelic dysplasia, autosomal sex-reversal
  • CMD 1
  • CMD1
  • CMPD1
  • SOX9
  • SRA1SRY (sex-determining region Y)-box 9 protein
  • SRY (sex determining region Y)-box 9
  • SRY-related HMG-box, gene 9
  • transcription factor SOX-9

Background

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX9 Antibody (AF3075)(31)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ChIP, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


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ChIP
(2)
ICC
(8)
IHC
(9)
IHC-Fr
(6)
IHC-P
(4)
Western Blot
(1)
All Applications
Filter By Species
Human
(16)
Mouse
(14)
Rat
(1)
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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using AF3075 Applications Species
OC Tysoe, AW Justin, T Brevini, SE Chen, KT Mahbubani, AK Frank, H Zedira, E Melum, K Saeb-Parsy, AE Markaki, L Vallier, F Sampazioti Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue Nat Protoc, 2019;14(6):1884-1925. 2019 [PMID: 31110298] (ICC, Human) ICC Human
SG Romeo, KM Alawi, J Rodrigues, A Singh, AP Kusumbe, SK Ramasamy Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation Nat. Cell Biol., 2019;21(4):430-441. 2019 [PMID: 30936475] (IHC-Fr, Mouse) IHC-Fr Mouse
MJ Castle, Y Cheng, A Asokan, MH Tuszynski Physical positioning markedly enhances brain transduction after intrathecal AAV9 infusion Sci Adv, 2018;4(11):eaau9859. 2018 [PMID: 30443600] (IHC, Rat) IHC Rat
JR Jones, L Kong, MG Hanna, B Hoffman, R Krencik, R Bradley, T Hagemann, J Choi, M Doers, M Dubovis, MA Sherafat, A Bhattachar, C Kendziorsk, A Audhya, A Messing, SC Zhang Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes Cell Rep, 2018;25(4):947-958.e4. 2018 [PMID: 30355500] (ICC, Human) ICC Human
Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30244870] (Human) Human
PL Lewis, J Su, M Yan, F Meng, SS Glaser, GD Alpini, RM Green, B Sosa-Pined, RN Shah Complex bile duct network formation within liver decellularized extracellular matrix hydrogels Sci Rep, 2018;8(1):12220. 2018 [PMID: 30111800] (IHC-Fr, Human) IHC-Fr Human
JM Rosin, DM Kurrasch In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus J Neuroinflammation, 2018;15(1):181. 2018 [PMID: 29895301] (IHC-Fr, Mouse) IHC-Fr Mouse
II Aigha, B Memon, AK Elsayed, EM Abdelalim Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors Stem Cell Res Ther, 2018;9(1):83. 2018 [PMID: 29615106] (ICC, Human) ICC Human
PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity Dev. Cell, 2018;44(6):679-693.e5. 2018 [PMID: 29587142] (IHC-Fr, Human) IHC-Fr Human
M Zhang, J Ngo, F Pirozzi, YP Sun, A Wynshaw-Bo Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells Stem Cell Res Ther, 2018;9(1):67. 2018 [PMID: 29544541] (ICC, Human) ICC Human
Show All 31 Publications.

Reviews for SOX9 Antibody (AF3075) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using AF3075:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin SOX9 AF3075
Enlarge
reviewed by:
Paula Aliberti
IHC-P Human 10/09/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman testis
SpeciesHuman
LotWIL0416121
 
reviewed by:
Anonymous
IF Rat 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22791629
SpeciesRat

Blocking

Blocking DetailsSee PMID 22791629

Details

Detection NotesSee PMID 22791629

Comments

CommentsPublished: See PMID 22791629Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOX9 Antibody (AF3075) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF3075
NL557 NL3075R

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Bioinformatics Tool for SOX9 Antibody (AF3075)

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Diseases for SOX9 Antibody (AF3075)

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Pathways for SOX9 Antibody (AF3075)

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Blogs on SOX9.

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients
By Michalina Hanzel, PhDAlexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

SOX9 - Be careful, I can reverse your gender!
SOX9 is a member of the SOX family of HMG DNA-binding domain transcription factors. The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. cAMP and protein kinase A (PKA) st...  Read full blog post.

Using the Hif-1 Alpha Antibody in Prostate Cancer Research
The Hypoxia-inducible Factor 1 (HIF1) protein is a heterodimeric transcription factor which plays an important role in mammalian oxygen homeostasis in conditions of hypoxia, or low oxygen concentration. HIF-1 alpha antibody reagents are widely used in...  Read full blog post.

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Recent Reviews

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5
2
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1
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Paula Aliberti
10/09/2017
Application: IHC-P
Species: Human

 
Anonymous
01/07/2015
Application: IF
Species: Rat

Bioinformatics

Gene Symbol SOX9
Uniprot