SOX9 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO‑III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular ...read more
SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells ...read more
SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) ...read more
Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for SOX9 at approximately 107 kDa (as indicated) using 20 µg/mL of Goat ...read more
Comparison of E12.5 Sox9IE/IE and Sox9FE/FE embryos based on EGFP intensity and Western blotting.(A) Left panel: white light images of E12.5 Sox9IE/IE and Sox9FE/FE embryos; right panel: same embryos imaged by ...read more
Dbx1‐expressing progenitors give rise to preBötC neurons and glia. A. Confocal images of Dbx1CreERT2; Rosa26tdTomato mouse preBötC sections immunostained for NeuN. Dbx1‐derived neurons (white arrowhead) expressed ...read more
Feeder-free expansion of SOX9+ BCs. (A) Immunostaining of SOX9+ BCs with anti-P63, anti-KRT5 and anti-SOX9 antibodies. (B) SOX9+ BCs in rugae of 3rd order human airway by anti-SOX9, anti-P63 and anti-CC10 ...read more
Transplantated SOX9+ BCs regenerate functional human lungin vivo. (A) Left, direct fluorescence image under stereomicroscope showing NOD-SCID mouse lung without (upper panel) or with (lower panel) GFP-labeled SOX9+ BC ...read more
Feeder-free expansion of SOX9+ BCs. (A) Immunostaining of SOX9+ BCs with anti-P63, anti-KRT5 and anti-SOX9 antibodies. (B) SOX9+ BCs in rugae of 3rd order human airway by anti-SOX9, anti-P63 and anti-CC10 ...read more
Feeder-free expansion of SOX9+ BCs. (A) Immunostaining of SOX9+ BCs with anti-P63, anti-KRT5 and anti-SOX9 antibodies. (B) SOX9+ BCs in rugae of 3rd order human airway by anti-SOX9, anti-P63 and anti-CC10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

SOX9 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX9
Met1-Lys151
Accession # P48436
Specificity
Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human SOX10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF3075 in the following applications:

Publications
Read Publications using
AF3075 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX9 Antibody [Unconjugated]

  • campomelic dysplasia, autosomal sex-reversal
  • CMD 1
  • CMD1
  • CMPD1
  • SOX9
  • SRA1SRY (sex-determining region Y)-box 9 protein
  • SRY (sex determining region Y)-box 9
  • SRY-related HMG-box, gene 9
  • transcription factor SOX-9

Background

SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX9 Antibody (AF3075)(83)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Transgenic Mouse, Xenograft.

We have publications tested in 8 applications: ChIP, ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ChIP
(2)
ICC
(19)
ICC/IF
(1)
IF
(1)
IHC
(49)
IHC-Fr
(8)
IHC-P
(4)
Western Blot
(3)
All Applications
Filter By Species
Human
(32)
Mouse
(46)
Rat
(3)
Chicken
(1)
Transgenic Mouse
(2)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 83. Show All 83 Publications.
Publications using AF3075 Applications Species
Hassan, D;Chen, J; CEBPA restricts alveolar type 2 cell plasticity during development and injury-repair bioRxiv : the preprint server for biology 2023-10-12 [PMID: 37873326] (IHC, Mouse) IHC Mouse
Szewczyk, LM;Lipiec, MA;Liszewska, E;Meyza, K;Urban-Ciecko, J;Kondrakiewicz, L;Goncerzewicz, A;Rafalko, K;Krawczyk, TG;Bogaj, K;Vainchtein, ID;Nakao-Inoue, H;Puscian, A;Knapska, E;Sanders, SJ;Jan Nowakowski, T;Molofsky, AV;Wisniewska, MB; Astrocytic ?-catenin signaling via TCF7L2 regulates synapse development and social behavior Molecular psychiatry 2023-10-05 [PMID: 37798419] (ICC, IHC, Mouse) ICC, IHC Mouse
Niu, C;Yue, X;An, JJ;Xu, H;Xu, B; Genetic dissection of BDNF and TrkB expression in glial cells bioRxiv : the preprint server for biology 2023-07-25 [PMID: 37503044] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Matkovic Leko, I;Schneider, RT;Thimraj, TA;Schrode, N;Beitler, D;Liu, HY;Beaumont, K;Chen, YW;Snoeck, HW; A distal lung organoid model to study interstitial lung disease, viral infection and human lung development Nature protocols 2023-05-10 [PMID: 37165073] (IHC, Human) IHC Human
Q Li, Y Qiao, F Wang, J Zhao, L Wu, H Ge, S Xu Prenatal triclosan exposure impairs mammalian lung branching morphogenesis through activating Bmp4 signaling Ecotoxicology and environmental safety, 2023-04-12;256(0):114896. 2023-04-12 [PMID: 37054474] (IHC, Mouse) IHC Mouse
J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation Cell Reports, 2023-01-05;42(1):111942. 2023-01-05 [PMID: 36640327] (IHC, Human) IHC Human
Y Wang, S Zhang, Z Lan, V Doan, B Kim, S Liu, M Zhu, VL Hull, S Rihani, CL Zhang, JA Gray, F Guo SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice Cell Reports, 2022-12-20;41(12):111842. 2022-12-20 [PMID: 36543123] (IHC, Mouse) IHC Mouse
K Zhang, E Yao, E Chuang, B Chen, EY Chuang, PT Chuang mTORC1 signaling facilitates differential stem cell differentiation to shape the developing murine lung and is associated with mitochondrial capacity Nature Communications, 2022-11-25;13(1):7252. 2022-11-25 [PMID: 36433959] (IHC, Mouse) IHC Mouse
O Revah, F Gore, KW Kelley, J Andersen, N Sakai, X Chen, MY Li, F Birey, X Yang, NL Saw, SW Baker, ND Amin, S Kulkarni, R Mudipalli, B Cui, S Nishino, GA Grant, JK Knowles, M Shamloo, JR Huguenard, K Deisseroth, SP Pa?ca Maturation and circuit integration of transplanted human cortical organoids Nature, 2022-10-12;610(7931):319-326. 2022-10-12 [PMID: 36224417] (ICC, Xenograft) ICC Xenograft
Y Tian, W Wang, S Lautrup, H Zhao, X Li, PWN Law, ND Dinh, EF Fang, HH Cheung, WY Chan WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome Nature Communications, 2022-09-16;13(1):5456. 2022-09-16 [PMID: 36114168] (ICC, Human) ICC Human
Show All 83 Publications.

Reviews for SOX9 Antibody (AF3075) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using AF3075:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin SOX9 AF3075
Enlarge
5
reviewed by:
Paula Aliberti
IHC-P Human 10/09/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman testis
SpeciesHuman
LotWIL0416121
  5
reviewed by:
Verified Customer
IF Rat 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22791629
SpeciesRat

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOX9 Antibody (AF3075) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SOX9 Products

Blogs on SOX9.

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients
By Michalina Hanzel, PhDAlexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an ...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

SOX9 - Be careful, I can reverse your gender!
SOX9 is a member of the SOX family of HMG DNA-binding domain transcription factors. The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. cAMP and protein kinase A (PKA) st...  Read full blog post.

Using the Hif-1 Alpha Antibody in Prostate Cancer Research
The Hypoxia-inducible Factor 1 (HIF1) protein is a heterodimeric transcription factor which plays an important role in mammalian oxygen homeostasis in conditions of hypoxia, or low oxygen concentration. HIF-1 alpha antibody reagents are widely used in...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Paula Aliberti
10/09/2017
Application: IHC-P
Species: Human

 
Verified Customer
01/07/2015
Application: IF
Species: Rat

Bioinformatics

Gene Symbol SOX9
Uniprot