Reactivity | HuSpecies Glossary |
Applications | WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP |
Clone | 6F-H2 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 1.0 mg/ml |
Immunogen | Human recombinant Wilms Tumor 1 protein (WT1) (residues 1-181). [Swiss-Prot: P19544] |
Localization | Nuclear, Cytoplasm |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | WT1 |
Purity | Protein G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | In Western blot, a doublet is observed at approx. 55 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In immunostaining, this target may be found localized to nucleus/nucleolus and cytoplasm of cells (WT1 shuttles between nucleus and cytoplasm - PMID: 14681305). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25576053). |
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Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Reviewed Applications |
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Publications |
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Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.02% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Protein G purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||||||||
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Enlarge |
reviewed by:
Anonymous |
WB | Mouse and Human | 10/17/2019 |
Summary
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Enlarge |
reviewed by:
Santhosh Sivajothi |
IHC-P | Human | 05/22/2018 |
Summary
Comments
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Enlarge |
reviewed by:
Evren Azeloglu |
WB | Human | 04/28/2014 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
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Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NB110-60011AF350 | |
Alexa Fluor 405 | NB110-60011AF405 | |
Alexa Fluor 488 | NB110-60011AF488 | |
Alexa Fluor 532 | NB110-60011AF532 | |
Alexa Fluor 594 | NB110-60011AF594 | |
Alexa Fluor 647 | NB110-60011AF647 | |
Alexa Fluor 700 | NB110-60011AF700 | |
Alexa Fluor 750 | NB110-60011AF750 | |
Biotin | NB110-60011B | |
DyLight 350 | NB110-60011UV | |
DyLight 405 | NB110-60011V | |
DyLight 488 | NB110-60011G | |
DyLight 550 | NB110-60011R | |
DyLight 594 | NB110-60011DL594 | |
DyLight 650 | NB110-60011C | |
DyLight 680 | NB110-60011FR | |
DyLight 755 | NB110-60011IR | |
FITC | NB110-60011F | |
HRP | NB110-60011H | |
Janelia Fluor 549 | NB110-60011JF549 | |
Janelia Fluor 646 | NB110-60011JF646 |
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Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme Enzyme antibodies are widely used in both apoptosis and cancer studies, as disruption of the proteins regulating apoptosis is known to lead to formation of tumor cells in certain cancers. We at Novus Biologicals are continually growing our enzyme anti... Read full blog post. |
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Anonymous 10/17/2019 |
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Application: | WB | |
Species: | Mouse and Human |
Santhosh Sivajothi 05/22/2018 |
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Application: | IHC-P | |
Species: | Human |
Evren Azeloglu 04/28/2014 |
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Application: | WB | |
Species: | Human |
Gene Symbol | WT1 |