WT1 Antibody (6F-H2)


Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - WT1 in 293 cell lysate
Immunohistochemistry: WT1 Antibody (6F-H2) [NB110-60011] - IHC analysis of Wilms Tumor 1 in human renal cancer using DAB with hematoxylin counterstain.
Simple Western: WT1 Antibody (6F-H2) [NB110-60011] - Simple Western lane view shows a specific band for WT1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, IHC-Fr, IHC-P, IP
0.8 mg/ml

WT1 Antibody (6F-H2) Summary

Human recombinant Wilms Tumor 1 protein (residues 1-181). [Swiss-Prot# P19544]
Nuclear, Cytoplasm
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
0.8 mg/ml
Protein G purified


  • Western Blot 2 ug/ml
  • Simple Western 1:50
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen 1: 400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 2-10 ug/ml lysate
Application Notes
This Wilms Tumor 1 antibody is useful for Immunohistochemistry paraffin embedded / frozen sections, Western blot and Immunoprecipitation. In WB, a doublet is observed ~55 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In immunostaining, this target may be found localized to Nucleus/nucleolus and Cytoplasm of cells (WT1 shuttles between nucleus and cytoplasm - PMID 14681305).In Simple Western only 10-15 uL of the recommended dilution is used per data point.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
NB110-60011 in the following applications:

Read Publications using
NB110-60011 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and Mouse.

Alternate Names for WT1 Antibody (6F-H2)

  • AWT1
  • GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT33


Wilms' tumor 1 (WT1) gene inactivation is responsible for almost 20% of Wilms' tumour/developmental renal tumour or nephroblastoma cases and besides playing important role in cellular development and survival, this homodimeric transcription factor has been shown to involve in certain pathologies also. WT1 belongs to EGR C2H2-type zinc-finger protein family and interacts with several proteins including WTIP, HNRNPU, U2AF2, CITED2, ZNF224, WTAP, SRY, AMER1, RBM4 and actively translating polysomes (detected in nuclear ribonucleoprotein particles) for exerting its activities. WT1 binds to DNA sequence 5'-CGCCCCCGC-3' and regulates the expression of several target genes including EPO. It plays essential role in urogenital system development and in carcinogenesis, it has been suggested as tumor suppressor as well as oncogenic protein. Defects in WT1 have been linked to Wilms tumor 1 (WT1), Frasier syndrome (FS), Denys-Drash syndrome (DDS), nephrotic syndrome type 4 (NPHS4), Meacham syndrome (MEACHS), mesothelioma malignant (MESOM).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WT1 Antibody (NB110-60011)(4)

Reviews for WT1 Antibody (NB110-60011) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB110-60011:
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Images Ratings Applications Species Date Details
Western Blot WT1 NB110-60011
reviewed by:
WB Human 04/28/2014


ApplicationWestern Blot
Sample TestedImmortalized podocytes


Blocking DetailsRockland casein blocking buffer, 2 hrs, RT

Primary Anitbody

Dilution Ratio1:1000 in 5% milk in TBS-T, overnight, 4ºC

Secondary Antibody

Secondary DescriptionThermo goat anti-rabbit HRP
Secondary Concentration1:2000 in 5% milk in TBS-T, 30min, RT


Detection NotesThermo ECL, 2min

Product General Protocols

View specific protocols for WT1 Antibody (NB110-60011): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for WT1 Antibody (NB110-60011). (Showing 1 - 2 of 2 FAQs).

  1. I just wondering this antibody (WT1 antibody (6F-H2)[Alexa Flour488], when I use it to staining tissue do I need incubate in second antibody or not?
    • Our product NB110-60011AF488 is directly conjugated with an Alexa Fluor 488 dye, so you do not need to use any secondary antibody with this product to detect the antibody. The dye labeled to this antibody excites at 490nm and emits at 525nm.
  2. In NB800-PC6, were the 293 cells transfected with WT1 expression plasmids? We have 293 cell lysates and it showed negative WT1 expression. Just want to make sure if our antibody is not working.
    • This protein lysate was derived from human HEK293 cells (without any transfection) and it is a whole cell lysate. WT1's mRNA is expressed significantly in HEK294 cells protein atlas site and given the fact that our Wilms Tumor 1 antibody (6F-H2) [NB110-60011] showed positive bands on HEK293 cells lysate, you should be good for considering this lysate as a positive control for WT1.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-60011AF405
Alexa Fluor (R) 488 Labeled NB110-60011AF488
Alexa Fluor (R) 647 Labeled NB110-60011AF647
Alexa Fluor (R) 700 Labeled NB110-60011AF700
Biotin Labeled NB110-60011B
DyLight 405 Labeled NB110-60011V
DyLight 488 Labeled NB110-60011G
DyLight 550 Labeled NB110-60011R
DyLight 650 Labeled NB110-60011C
FITC Labeled NB110-60011F
HRP Labeled NB110-60011H

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Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme
Enzyme antibodies are widely used in both apoptosis and cancer studies, as disruption of the proteins regulating apoptosis is known to lead to formation of tumor cells in certain cancers. We at Novus Biologicals are continually growing our enzyme anti...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol WT1

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