WT1 Antibody (6F-H2) - BSA Free

Images

 
Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from ...read more
Immunohistochemistry-Paraffin: WT1 Antibody (6F-H2) [NB110-60011] - Staining of human kidney glomerulus tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. ...read more
Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Whole cell protein from human K562, HEK293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunohistochemistry: WT1 Antibody (6F-H2) [NB110-60011] - Analysis of Wilms Tumor 1 in human renal cancer using DAB with hematoxylin counterstain.
Simple Western: WT1 Antibody (6F-H2) [NB110-60011] - Lane view shows a specific band for WT1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP
Clone
6F-H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

WT1 Antibody (6F-H2) - BSA Free Summary

Immunogen
Human recombinant Wilms Tumor 1 protein (WT1) (residues 1-181). [Swiss-Prot: P19544]
Localization
Nuclear, Cytoplasm
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 25576053)
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen 1: 400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 2-10 ug/ml lysate
  • Simple Western 1:50
  • Western Blot 2 ug/ml
Application Notes
In Western blot, a doublet is observed at approx. 55 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In immunostaining, this target may be found localized to nucleus/nucleolus and cytoplasm of cells (WT1 shuttles between nucleus and cytoplasm - PMID: 14681305). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
293 Whole Cell Lysate
K-562 Whole Cell Lysate
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB110-60011 in the following applications:

Publications
Read Publications using
NB110-60011 in the following applications:

  • 4 publications
  • IHC
    4 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for WT1 Antibody (6F-H2) - BSA Free

  • AWT1
  • GUD
  • NPHS4GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT1
  • WT33

Background

Wilms' tumor 1 (WT1) gene inactivation is responsible for almost 20% of Wilms' tumour/developmental renal tumour or nephroblastoma cases and besides playing important role in cellular development and survival, this homodimeric transcription factor has been shown to involve in certain pathologies also. WT1 belongs to EGR C2H2-type zinc-finger protein family and interacts with several proteins including WTIP, HNRNPU, U2AF2, CITED2, ZNF224, WTAP, SRY, AMER1, RBM4 and actively translating polysomes (detected in nuclear ribonucleoprotein particles) for exerting its activities. WT1 binds to DNA sequence 5'-CGCCCCCGC-3' and regulates the expression of several target genes including EPO. It plays essential role in urogenital system development and in carcinogenesis, it has been suggested as tumor suppressor as well as oncogenic protein. Defects in WT1 have been linked to Wilms tumor 1 (WT1), Frasier syndrome (FS), Denys-Drash syndrome (DDS), nephrotic syndrome type 4 (NPHS4), Meacham syndrome (MEACHS), mesothelioma malignant (MESOM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WT1 Antibody (NB110-60011)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(4)
IHC
(4)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB110-60011 Applications Species
Yang X, Chen Z, Luo Z et al. STING deletion alleviates podocyte injury through suppressing inflammation by targeting NLRP3 in diabetic kidney disease Cellular signalling 2023-06-15 [PMID: 37329999] (IHC, Mouse) IHC Mouse
Hu J, Zhang Z, Hu H et al. LRH-1 activation alleviates diabetes-induced podocyte injury by promoting GLS2-mediated glutaminolysis Cell proliferation 2023-04-13 [PMID: 37057309] (IHC, Mouse) IHC Mouse
Cao Y, Chen Z, Hu J et al. Mfn2 Regulates High Glucose-Induced MAMs Dysfunction and Apoptosis in Podocytes via PERK Pathway Frontiers in Cell and Developmental Biology 2021-12-20 [PMID: 34988075] (ICC/IF) ICC/IF
Loubalova Z, Fulka H, Horvat F et al. Formation of spermatogonia and fertile oocytes in golden hamsters requires piRNAs Nature Cell Biology 2021-09-06 [PMID: 34489573] (IHC, ICC/IF) IHC, ICC/IF
Horne SJ, Vasquez J, Guo Y et al. Podocyte-Specific Loss of Kruppel-Like Factor 6 Increases Mitochondrial Injury in Diabetic Kidney Disease Diabetes Aug 16 2018 [PMID: 30115650] (IHC, Mouse) IHC Mouse
Fan Ying, Li Xuezhu, Xiao Wenzhen et al. BAMBI elimination enhances alternative TGF-b signaling and glomerular dysfunction in diabetic mice. Diabetes 2015 [PMID: 25576053] (ICC/IF, Mouse) ICC/IF Mouse
Jeitany M, Pineda JR, Liu Q et al. A preclinical mouse model of glioma with an alternative mechanism of telomere maintenance (ALT). Int. J Cancer. 2014-08-30 [PMID: 25175359]
McCarty G, Awad O, Loeb DM. WT1 directly regulates expression of vascular endothelial growth factor and is a mediator of tumor response to hypoxia. The Journal of Biological Chemistry. 2011-10-26 [PMID: 22030397]
Rauscher III, FJ et al. Characterization of monoclonal antibodies directed to the amino-terminus of the WT1, Wilms' tumor suppressor protein. Hybridoma;17(2):191-8. 1998-04-01 [PMID: 9627060]
Loubalova Z, Fulka H, Horvat F, et al. Golden hamster piRNAs are necessary for early embryonic development and establishment of spermatogonia bioRxiv 2021-01-28 (ICC/IF, Hamster) ICC/IF Hamster
Show All 14 Publications.

Reviews for WT1 Antibody (NB110-60011) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Human and Mouse.

Reviews using NB110-60011:
Filter by Applications
WB
(2)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot WT1 NB110-60011
Enlarge
5
reviewed by:
Verified Customer
WB Human and Mouse 10/17/2019
View

Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate,Liver
SpeciesHuman and Mouse
LotB-4
Immunohistochemistry-Paraffin WT1 NB110-60011
Enlarge
4
reviewed by:
Santhosh Sivajothi
IHC-P Human 05/22/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman kidney tissue
SpeciesHuman
LotA-5

Comments

CommentsHeat mediated antigen retrieval was performed by heating in citrate buffer (pH6) at 95C for 20 minutes
Western Blot WT1 NB110-60011
Enlarge
4
reviewed by:
Evren Azeloglu
WB Human 04/28/2014
View

Summary

ApplicationWestern Blot
Sample TestedImmortalized podocytes
SpeciesHuman

Product General Protocols

View specific protocols for WT1 Antibody (NB110-60011): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WT1 Antibody (NB110-60011). (Showing 1 - 2 of 2 FAQs).

  1. I just wondering this antibody (WT1 antibody (6F-H2)[Alexa Flour488], when I use it to staining tissue do I need incubate in second antibody or not?
    • Our product NB110-60011AF488 is directly conjugated with an Alexa Fluor 488 dye, so you do not need to use any secondary antibody with this product to detect the antibody. The dye labeled to this antibody excites at 490nm and emits at 525nm.
  2. In NB800-PC6, were the 293 cells transfected with WT1 expression plasmids? We have 293 cell lysates and it showed negative WT1 expression. Just want to make sure if our antibody is not working.
    • This protein lysate was derived from human HEK293 cells (without any transfection) and it is a whole cell lysate. WT1's mRNA is expressed significantly in HEK294 cells protein atlas site and given the fact that our Wilms Tumor 1 antibody (6F-H2) [NB110-60011] showed positive bands on HEK293 cells lysate, you should be good for considering this lysate as a positive control for WT1.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional WT1 Products

Research Areas for WT1 Antibody (NB110-60011)

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Blogs on WT1.

Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme
Enzyme antibodies are widely used in both apoptosis and cancer studies, as disruption of the proteins regulating apoptosis is known to lead to formation of tumor cells in certain cancers. We at Novus Biologicals are continually growing our enzyme anti...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Verified Customer
10/17/2019
Application: WB
Species: Human and Mouse

Santhosh Sivajothi
05/22/2018
Application: IHC-P
Species: Human

Evren Azeloglu
04/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol WT1