SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101)

Images

 
Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Staining of U-2 OS cells using the Anti-NR5A1 monoclonal antibody, showing specific staining in the nucleoplasm ...read more
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Staining of human testis shows weak to moderate nuclear positivity in Leydig cells and cells in seminiferous ducts.
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Staining of human adrenal gland shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Staining of human breast shows weak to moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Staining of human ovary shows moderate positivity in stromal cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
CL9101
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids: PYGYPEPFSGGPNVP
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NR5A1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101)

  • AD4BP
  • AD4BPSTF-1
  • Adrenal 4-binding protein
  • ELP
  • FTZ1adrenal 4 binding protein
  • FTZF1
  • FTZF1nuclear receptor AdBP4
  • Fushi tarazu factor homolog 1
  • NR5A1
  • Nuclear receptor subfamily 5 group A member 1
  • nuclear receptor subfamily 5, group A, member 1
  • SF1
  • SF-1
  • SF-1POF7
  • SF1steroidogenic factor 1
  • Steroid hormone receptor Ad4BP
  • steroidogenic factor-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for SF-1/NR5A1/Steroidogenic Factor 1 Antibody (NBP2-88921)

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Bioinformatics

Gene Symbol NR5A1