StAR Antibody

Images

 
Western Blot: StAR Antibody [NBP1-33485] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:5000. The ...read more
Immunocytochemistry/ Immunofluorescence: StAR Antibody [NBP1-33485] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR protein stained by StAR antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: StAR Antibody [NBP1-33485] - Human hepatoma. StAR antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: StAR Antibody [NBP1-33485] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Western Blot: StAR Antibody [NBP1-33485] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Po, Ca, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

StAR Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human StAR. The exact sequence is proprietary.
Localization
Mitochondrion
Predicted Species
Porcine (90%), Rhesus Macaque (98%), Canine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STAR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
StAR Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP1-33485 in the following applications:

  • WB
    2 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (Bidne KL et al).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for StAR Antibody

  • cholesterol trafficker
  • mitochondrial steroid acute regulatory protein
  • StAR
  • StARD1
  • STARD1StAR-related lipid transfer (START) domain containing 1
  • START domain containing 1
  • START domain-containing protein 1
  • steroid acute regulatory protein
  • steroidogenic acute regulator
  • steroidogenic acute regulatory protein
  • steroidogenic acute regulatory protein, mitochondrial

Background

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for StAR Antibody (NBP1-33485)(3)

We have publications tested in 2 confirmed species: Mouse, Porcine.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Mouse
(1)
Porcine
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for StAR Antibody (NBP1-33485) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Contact Information

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Bioinformatics

Gene Symbol STAR