StAR Antibody

Western Blot: StAR Antibody [NBP1-33485] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunocytochemistry/Immunofluorescence: StAR Antibody [NBP1-33485] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR protein stained by StAR antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: StAR Antibody [NBP1-33485] - Human hepatoma. StAR antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: StAR Antibody [NBP1-33485] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: StAR Antibody [NBP1-33485] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody (NBP1-33485) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: StAR Antibody [NBP1-33485] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Ca, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

StAR Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human StAR. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Rhesus Macaque (98%), Canine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:100-1:10000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported in scientific literature (Bidne KL et al).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for StAR Antibody

• cholesterol trafficker
• mitochondrial steroid acute regulatory protein
• StAR
• StARD1
• STARD1StAR-related lipid transfer (START) domain containing 1
• START domain containing 1
• START domain-containing protein 1
• steroid acute regulatory protein
• steroidogenic acute regulator
• steroidogenic acute regulatory protein
• steroidogenic acute regulatory protein, mitochondrial

Background

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for StAR Antibody (NBP1-33485)(5)

Publications using NBP1-33485

• Jiang JH, Wang YF, Zheng J et Al. Human-like adrenal features in Chinese tree shrews revealed by multi-omics analysis of adrenal cell populations and steroid synthesis Zool Res 2024-05-20 [PMID: 38766745]
• Wang Y, Guo B, Guo Y, Qi N et Al. A spatiotemporal steroidogenic regulatory network in human fetal adrenal glands and gonads Front Endocrinol (Lausanne) 2022-12-05 [PMID: 36465633]
• Bidne KL. Investigating the ovarian response to endotoxemia. Thesis 1905-07-09 (WB, Porcine)
• Ganesan S, Keating AF Ovarian mitochondrial and oxidative stress proteins are altered by glyphosate exposure in mice Toxicol. Appl. Pharmacol. 2020-07-04 [PMID: 32634520] (WB, Mouse)
• Bidne KL, Romoser MR, Ross JW et al. Heat stress during the luteal phase decreases luteal size but does not impact circulating progesterone in gilts J. Anim. Sci. 2019-08-02 [PMID: 31372640]

Bioinformatics

• Gene Symbol: STAR