BRD2 Antibody

Images

 
Western Blot: BRD2 Antibody [NBP1-30475] - Detection of Human and Mouse BRD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: ...read more
Immunocytochemistry/ Immunofluorescence: BRD2 Antibody [NBP1-30475] - Affinity purified rabbit anti-BRD2 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody ...read more
Immunohistochemistry: BRD2 Antibody [NBP1-30475] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 used at a dilution of 1:50 (4ug/ml). Detection: DAB staining using ...read more
Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate from HeLa and 293T cells. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30474.
Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.2 ug/ml.
Proximity Ligation Assay: BRD2 Antibody [NBP1-30475] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human BRD2 (NBP1-30475). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NBP1-30475] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human DMAP1 (NB100-256) and rabbit anti-human BRD2 (NBP1-30475). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: BRD2 Antibody [NBP1-30475] - image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

BRD2 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 675 and 725 of human bromodomain containing 2 using the numbering given in entry NP_005095.1 (GeneID 6046).
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:25 - 1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:200-1:4000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRD2 Antibody

  • bromodomain containing 2
  • bromodomain-containing 2
  • bromodomain-containing protein 2
  • D6S113E
  • female sterile homeotic-related gene 1
  • FSRG1FSH
  • KIAA9001FLJ31942
  • NAT
  • O27.1.1
  • Really interesting new gene 3 protein
  • RING3DKFZp686N0336
  • RNF3

Background

BRD2 (bromodomain-containing 2) is a nuclear mitogen-activated kinase that plays a role in transcription of cell-cycle-regulated genes by functioning as an adapter protein that recruits E2F and chromatin remodeling factors to E2F response elements. Alternate names of BRD2 include RING3, NAT, RNF3, FSRG1, D6S113E, FLJ31942, DKFZp686N0336, and KIAA9001.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BRD2
Entrez
Uniprot