GAD1/GAD67 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen ...read more
GAD1/GAD67 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) overnight ...read more
Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GAD1/GAD67 at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GAD1/GAD67 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259
Specificity
Detects human GAD1/GAD67 in direct ELISAs and detects human, mouse, and rat GAD1/GAD67 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GAD1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2086 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAD1/GAD67 Antibody [Unconjugated]

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

GAD1, also named 67 kDa or brain GAD, is an enzyme that catalyzes the formation of the inhibitory neurotransmitter gamma -amino butyric acid (GABA) from glutamate. GAD1 is also expressed in multiple non-neuronal tissues during development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAD1/GAD67 Antibody (AF2086)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 2 applications: IHC, IHC-Fr.


Filter By Application
IHC
(6)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF2086 Applications Species
Song, Y;Seward, CH;Chen, CY;LeBlanc, A;Leddy, AM;Stubbs, L; Isolated loss of the AUTS2 long isoform, brain-wide or targeted to Calbindin -lineage cells, generates a specific suite of brain, behavioral and molecular pathologies bioRxiv : the preprint server for biology 2023-05-06 [PMID: 37205596] (IHC, Transgenic Mouse) IHC Transgenic Mouse
S Chen, Y Chang, L Li, D Acosta, Y Li, Q Guo, C Wang, E Turkes, C Morrison, D Julian, ME Hester, DW Scharre, C Santiskulv, SX Song, JT Plummer, GE Serrano, TG Beach, KE Duff, Q Ma, H Fu Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer's disease Acta neuropathologica communications, 2022-12-21;10(1):188. 2022-12-21 [PMID: 36544231] (IHC, Human) IHC Human
S Meneghini, D Modena, G Colombo, A Coatti, N Milani, L Madaschi, A Amadeo, A Becchetti The beta2V287L nicotinic subunit linked to sleep-related epilepsy differently affects fast-spiking and regular spiking somatostatin-expressing neurons in murine prefrontal cortex Progress in neurobiology, 2022-05-02;0(0):102279. 2022-05-02 [PMID: 35513164] (IHC, Mouse) IHC Mouse
M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 2021-10-01;0(0):. 2021-10-01 [PMID: 34599305] (IHC, Human, Mouse) IHC Human, Mouse
Kenneth N Fish Alterations in a Unique Class of Cortical Chandelier Cell Axon Cartridges in Schizophrenia Biol. Psychiatry, 2016-09-29;0(0):. 2016-09-29 [PMID: 27884423] (IHC, Human) IHC Human
Mayerl S, Muller J, Bauer R, Richert S, Kassmann C, Darras V, Buder K, Boelen A, Visser T, Heuer H Transporters MCT8 and OATP1C1 maintain murine brain thyroid hormone homeostasis. J Clin Invest, 2014-04-01;124(5):1987-99. 2014-04-01 [PMID: 24691440] (IHC, Human) IHC Human
Wallis K, Sjogren M, van Hogerlinden M, Silberberg G, Fisahn A, Nordstrom K, Larsson L, Westerblad H, Morreale de Escobar G, Shupliakov O, Vennstrom B Locomotor deficiencies and aberrant development of subtype-specific GABAergic interneurons caused by an unliganded thyroid hormone receptor alpha1. J. Neurosci., 2008-02-20;28(8):1904-15. 2008-02-20 [PMID: 18287507] (IHC-Fr, Mouse) IHC-Fr Mouse

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Bioinformatics

Gene Symbol GAD1
Uniprot