GAD1/GAD67 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen ...read more
Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GAD1/GAD67 at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat ...read more
GAD1/GAD67 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) overnight ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

GAD1/GAD67 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259
Specificity
Detects human GAD1/GAD67 in direct ELISAs and detects human, mouse, and rat GAD1/GAD67 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human GAD2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GAD1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF2086 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAD1/GAD67 Antibody [Unconjugated]

  • 67kD)
  • EC 4.1.1
  • FLJ45882
  • GAD1
  • GAD25
  • GAD67
  • glutamate decarboxylase 1 (brain, 67kDa)
  • Glutamate decarboxylase 67 kDa isoform

Background

GAD1, also named 67 kDa or brain GAD, is an enzyme that catalyzes the formation of the inhibitory neurotransmitter gamma -amino butyric acid (GABA) from glutamate. GAD1 is also expressed in multiple non-neuronal tissues during development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GAD1/GAD67 Antibody (AF2086)(3)

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Bioinformatics

Gene Symbol GAD1
Entrez
Uniprot