GAD1/GAD67 Antibody [Unconjugated]

Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GAD1/GAD67 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GAD1/GAD67 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GAD1/GAD67 at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GAD1/GAD67 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GAD1/GAD67
  Ala2-Asn97
  Accession # Q99259
• Specificity: Detects human GAD1/GAD67 in direct ELISAs and detects human, mouse, and rat GAD1/GAD67 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GAD1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAD1/GAD67 Antibody [Unconjugated]

• 67kD)
• EC 4.1.1
• FLJ45882
• GAD1
• GAD25
• GAD67
• glutamate decarboxylase 1 (brain, 67kDa)
• Glutamate decarboxylase 67 kDa isoform

Background

GAD1, also named 67 kDa or brain GAD, is an enzyme that catalyzes the formation of the inhibitory neurotransmitter gamma -amino butyric acid (GABA) from glutamate. GAD1 is also expressed in multiple non-neuronal tissues during development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAD1/GAD67 Antibody (AF2086)(6)

Publications using AF2086

• S Chen, Y Chang, L Li, D Acosta, Y Li, Q Guo, C Wang, E Turkes, C Morrison, D Julian, ME Hester, DW Scharre, C Santiskulv, SX Song, JT Plummer, GE Serrano, TG Beach, KE Duff, Q Ma, H Fu Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer's disease Acta neuropathologica communications, 2022;10(1):188. 2022 [PMID: 36544231] (IHC, Human)
• S Meneghini, D Modena, G Colombo, A Coatti, N Milani, L Madaschi, A Amadeo, A Becchetti The beta2V287L nicotinic subunit linked to sleep-related epilepsy differently affects fast-spiking and regular spiking somatostatin-expressing neurons in murine prefrontal cortex Progress in neurobiology, 2022;0(0):102279. 2022 [PMID: 35513164] (IHC, Mouse)
• M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 2021;0(0):. 2021 [PMID: 34599305] (IHC, Human)
• Kenneth N Fish Alterations in a Unique Class of Cortical Chandelier Cell Axon Cartridges in Schizophrenia Biol. Psychiatry, 2016;0(0):. 2016 [PMID: 27884423] (IHC, Human)
• Mayerl S, Muller J, Bauer R, Richert S, Kassmann C, Darras V, Buder K, Boelen A, Visser T, Heuer H Transporters MCT8 and OATP1C1 maintain murine brain thyroid hormone homeostasis. J Clin Invest, 2014;124(5):1987-99. 2014 [PMID: 24691440] (IHC, Human)
• Wallis K, Sjogren M, van Hogerlinden M, Silberberg G, Fisahn A, Nordstrom K, Larsson L, Westerblad H, Morreale de Escobar G, Shupliakov O, Vennstrom B Locomotor deficiencies and aberrant development of subtype-specific GABAergic interneurons caused by an unliganded thyroid hormone receptor alpha1. J. Neurosci., 2008;28(8):1904-15. 2008 [PMID: 18287507] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: GAD1
• Uniprot:
  • Q99259()