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Synaptophysin Antibody

 (6 Publications)
 (2 Reviews)
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Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain dilution 1:100
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:00 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Rat ...read more
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Mouse Brain- ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Fe, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Synaptophysin Antibody Summary

Immunogen
Synthetic peptide taken within amino acid region 1-100 on human Synaptophysin protein.
Localization
Integral membrane protein. Synaptic vesicles.
Marker
Pre-synaptic marker
Specificity
This antibody does not cross-react with other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:125
Application Notes
Immunofluorescence reactivity reported in (PMID: 21368026). Immunoprecipitation reactivity reported in (PMID: 22970294). Synaptophysin antibody validated for IP, IHC-Frozen from verified customer reviews.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Rat Brain Whole Tissue Lysate (Adult Whole Normal)
Rat Pancreas Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-653 in the following applications:
Publications
Read Publications using
NB300-653 in the following applications:

  • ICC/IF
    1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Feline, Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Synaptophysin Antibody

  • Major synaptic vesicle protein p38
  • MRX
  • MRXSYP
  • Synaptophysin
  • SYP

Background

Synaptophysin is a 38 kDa synaptic vesicle associated protein. The pathology of synaptic components is investigated by measuring the expression of synaptophysin that is mainly concentrated in synaptic terminals. The expression of synaptophysin is a measure of dynamic plastic changes in nerve tissue. A decrease in synaptophysin and other synaptic vesicle associated proteins (Synaptotagmin, synaptosomal associated protein 25 kDa, SNAP-25, CLC ad GAP-43) generally means degeneration in the nerve fibers and an increased expression results in enhanced neuroplasticity and neuronal hypertrophy. In conditions involving generative changes in the nerve tissue, the expression of psynaptophysin is generally reduced, for example, in Schizophrenia an Alzhiemers disease. A decreased expression of synaptophysin in hippocampus of Schizoprenic patients has also been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(1 Publication)
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(1 Review)
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Publications for Synaptophysin Antibody (NB300-653)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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ICC/IF
(1)
IP
(1)
WB
(2)
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(2)
Mouse
(2)
Rat
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Reviews for Synaptophysin Antibody (NB300-653) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Rat, Feline.

Reviews using NB300-653:
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IHC-Fr
(1)
IP
(1)
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Rat
(1)
Feline
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Synaptophysin NB300-653
Enlarge 		5
reviewed by:
Anonymous
 IHC-Fr Feline 09/15/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRetina
SpeciesFeline
LotPld100lg
  4
reviewed by:
Brittany Weibley
 IP Rat 08/16/2010
View

Summary

ApplicationImmunoprecipitation
Sample TestedRat Retinal Tissue
SpeciesRat
CommentsLysate Amount: 150 ug, Ab Dilution Ratio: 1:1000

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: 2 hours, Primary Ab Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab Dilution: 1:4000

Details

Detection NotesSpecific Bands: Yes

Comments

CommentsLysate Amount: 150 ug, Ab Dilution Ratio: 1:1000

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Synaptophysin Antibody (NB300-653) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Rat Brain Whole Tissue Lysate (Adult Whole Normal)
Rat Pancreas Whole Tissue Lysate (Adult Whole Normal)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

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Synaptophysin a Marker Protein in Neuroendocrine Cells
Synaptophysin a Marker Protein in Neuroendocrine Cells Synaptophysin is a major integral membrane glycoprotein of neuronal synaptic vesicles present in virtually all synapses and shows a high degree of evolutionary conservation across the mammals. Syn...  Read full blog post.
Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.
Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.
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Immunohistochemistry-Frozen Synaptophysin NB300-653
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 Anonymous
09/15/2018
Application: IHC-Fr
Species: Feline
read this review
 
 Brittany Weibley
08/16/2010
Application: IP
Species: Rat
read this review

Bioinformatics

Gene Symbol SYP
Entrez
Uniprot