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Synaptophysin Antibody

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Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain dilution 1:100
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:00 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Rat ...read more
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Mouse Brain- ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Fe, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Synaptophysin Antibody Summary

Immunogen
Synthetic peptide taken within amino acid region 1-100 on human Synaptophysin protein.
Localization
Integral membrane protein. Synaptic vesicles.
Marker
Pre-synaptic marker
Specificity
This antibody does not cross-react with other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:125
  • Western Blot 1:500-1:1000
Application Notes
Immunofluorescence reactivity reported in (PMID: 21368026). Immunoprecipitation reactivity reported in (PMID: 22970294). Synaptophysin antibody validated for IP, IHC-Frozen from verified customer reviews.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB300-653 in the following applications:

  • ICC/IF
    1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Feline, Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Synaptophysin Antibody

  • Major synaptic vesicle protein p38
  • MRX
  • MRXSYP
  • Synaptophysin
  • SYP

Background

Synaptophysin is a 38 kDa synaptic vesicle associated protein. The pathology of synaptic components is investigated by measuring the expression of synaptophysin that is mainly concentrated in synaptic terminals. The expression of synaptophysin is a measure of dynamic plastic changes in nerve tissue. A decrease in synaptophysin and other synaptic vesicle associated proteins (Synaptotagmin, synaptosomal associated protein 25 kDa, SNAP-25, CLC ad GAP-43) generally means degeneration in the nerve fibers and an increased expression results in enhanced neuroplasticity and neuronal hypertrophy. In conditions involving generative changes in the nerve tissue, the expression of psynaptophysin is generally reduced, for example, in Schizophrenia an Alzhiemers disease. A decreased expression of synaptophysin in hippocampus of Schizoprenic patients has also been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - The frequency of chromogranin A positive hormone-negative (CPHN) cells decreases with age. Representative pancreatic sections from fetal (A) and infant-child (B-D) cases immunostained for Endocrine cocktail (insulin, glucagon, somatostatin, pancreatic polypeptide, and ghrelin) (white), chromogranin A (green), and DAPI (blue). Yellow arrows show CPHN cells. Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fendo.2018.00791/full), licensed under a CC-BY licence.
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Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
beta Amyloid
Antibody (MOAB-2) - B ...
NBP2-13075
Immunohistochemistry-Frozen: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Mouse brain (cerebral cortex). Red: MOAB-2 antibody staining, Blue: DAPI. Zeiss LSM800, 40x. Image from verified customer review.
Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - A plaque load in neocortex and hippocampus in APP/PS1 mice exposed to differential housing. Representative 10x MOAB-2 staining in hippocampus of APP/PS1 mice for each housing group. Environmental novelty exacerbates stress hormones and Aβ pathology in an Alzheimer's model. Sci Rep (2017)
Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Immunofluorescent detection of beta Amyloid with MOAB-2 in the subiculim of 1-, 2- and 4- month old 5xFAD mice. Scale bar 20 um (from PMID: 22423893).
(43 Publications)
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Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong diffused immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.
(1 Review)
(11 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Somatostatin
Antibody (7G5) - BSA
NBP2-37447
Immunofluorescence: Somatostatin Antibody (7G5) [NBP2-37447] - Paraffin-embedded human adult pancreas tissue. pH 9 antigen retrieval. Image from verified customer review.
Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.
ELISA: Somatostatin Antibody (7G5) [NBP2-37447] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
(2 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IF, IHC, IHC-P, WB
Fyn Antibody
NBP3-09063
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Immunocytochemistry/Immunofluorescence: Fyn Antibody [NBP3-09063] - ICC staining Fyn in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Fyn Antibody [NBP3-09063] - ICC staining Fyn in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
S100A1 Antibody
NBP1-87103
Western Blot: S100A1 Antibody [NBP1-87103] - Analysis in human cell line SK-MEL-30.
Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining of human heart muscle shows moderate to strong nuclear positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: S100A1 Antibody [NBP1-87103] - Staining in human heart muscle and lymph node tissues.. Corresponding S100A1 RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Procalcitonin
Antibody (44D9)
NB120-14817
Procalcitonin Antibody (44D9)
(8 Publications)
Species: Hu, Rt
Applications: ICC/IF, IHC, S-ELISA, WB

Publications for Synaptophysin Antibody (NB300-653)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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Showing Publications 1 - 7 of 7.
Publications using NB300-653 Applications Species
Sui L, Danzl N, Campbell SR et al. Beta Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells Diabetes 2017 Sep 20 [PMID: 28931519] (Human) Human
Lam CS, Tipoe GL, Wong JK et al. M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus. PLoS ONE. Nov 21 2016 [PMID: 27870896] (WB, Rat) WB Rat
D'Cruz TS, Weibley BN, Kimball SR, Barber AJ. Post-Translational Processing of Synaptophysin in the Rat Retina Is Disrupted by Diabetes PLoS One 2012 [PMID: 22970294] (IP, Rat) IP Rat
Postupna N, Rose SE, Bird TD et al. Novel antibody capture assay for paraffin-embedded tissue detects wide-ranging amyloid Beta and paired helical filament-tau accumulation in cognitively normal older adults Brain Pathol 2012 Jul [PMID: 21999410] (Human) Human
Dash PK, Gorantla S, Gendelman HE, et al. Loss of Neuronal Integrity during Progressive HIV-1 Infection of Humanized Mice. J Neurosci. [PMID: 21368026] (ICC/IF, Mouse) ICC/IF Mouse
Kilinc M, Creson TK, Rojas C et al. Splicing of the SynGAP Carboxyl-Terminus Enables Isoform-Specific Tuning of NMDA Receptor Signaling Linked to Cognitive Function bioRxiv Jan 1 2020 (WB, Mouse) WB Mouse
Erener S, Ellis CE, Ramzy A Et al. Deletion of pancreas-specific miR-216a reduces beta-cell mass and inhibits pancreatic cancer progression in mice Cell reports. Medicine Nov 16 2021 [PMID: 34841287]

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WB Video Protocol
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Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.
Synaptophysin a Marker Protein in Neuroendocrine Cells
Synaptophysin a Marker Protein in Neuroendocrine Cells Synaptophysin is a major integral membrane glycoprotein of neuronal synaptic vesicles present in virtually all synapses and shows a high degree of evolutionary conservation across the mammals. Syn...  Read full blog post.
Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.
Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.
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Bioinformatics

Gene Symbol SYP
Entrez
Uniprot