Synaptophysin Antibody


Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain dilution 1:100
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:00 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Rat more
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Mouse Brain- more

Product Details

Reactivity Hu, Mu, Rt, Ch, Fe, PmSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Order Details

Synaptophysin Antibody Summary

Synthetic peptide taken within amino acid region 1-100 on human Synaptophysin protein.
Integral membrane protein. Synaptic vesicles.
Pre-synaptic marker
This antibody does not cross-react with other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:125
  • Western Blot 1:500-1:1000
Application Notes
Immunofluorescence reactivity reported in (PMID: 21368026). Immunoprecipitation reactivity reported in (PMID: 22970294). Synaptophysin antibody validated for IP, IHC-Frozen from verified customer reviews.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB300-653 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Feline, Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for Synaptophysin Antibody

  • Major synaptic vesicle protein p38
  • MRX
  • Synaptophysin
  • SYP


Synaptophysin is a 38 kDa synaptic vesicle associated protein. The pathology of synaptic components is investigated by measuring the expression of synaptophysin that is mainly concentrated in synaptic terminals. The expression of synaptophysin is a measure of dynamic plastic changes in nerve tissue. A decrease in synaptophysin and other synaptic vesicle associated proteins (Synaptotagmin, synaptosomal associated protein 25 kDa, SNAP-25, CLC ad GAP-43) generally means degeneration in the nerve fibers and an increased expression results in enhanced neuroplasticity and neuronal hypertrophy. In conditions involving generative changes in the nerve tissue, the expression of psynaptophysin is generally reduced, for example, in Schizophrenia an Alzhiemers disease. A decreased expression of synaptophysin in hippocampus of Schizoprenic patients has also been reported.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: IHC, IHC-P, WB
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Applications: ICC/IF, IHC, S-ELISA, WB

Publications for Synaptophysin Antibody (NB300-653)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IP, WB.

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Showing Publications 1 - 7 of 7.
Publications using NB300-653 Applications Species
Sui L, Danzl N, Campbell SR et al. Beta Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells Diabetes 2017 Sep 20 [PMID: 28931519] (Human) Human
Lam CS, Tipoe GL, Wong JK et al. M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus. PLoS ONE. Nov 21 2016 [PMID: 27870896] (WB, Rat) WB Rat
D'Cruz TS, Weibley BN, Kimball SR, Barber AJ. Post-Translational Processing of Synaptophysin in the Rat Retina Is Disrupted by Diabetes PLoS One 2012 [PMID: 22970294] (IP, Rat) IP Rat
Postupna N, Rose SE, Bird TD et al. Novel antibody capture assay for paraffin-embedded tissue detects wide-ranging amyloid Beta and paired helical filament-tau accumulation in cognitively normal older adults Brain Pathol 2012 Jul [PMID: 21999410] (Human) Human
Dash PK, Gorantla S, Gendelman HE, et al. Loss of Neuronal Integrity during Progressive HIV-1 Infection of Humanized Mice. J Neurosci. [PMID: 21368026] (ICC/IF, Mouse) ICC/IF Mouse
Kilinc M, Creson TK, Rojas C et al. Splicing of the SynGAP Carboxyl-Terminus Enables Isoform-Specific Tuning of NMDA Receptor Signaling Linked to Cognitive Function bioRxiv Jan 1 2020 (WB, Mouse) WB Mouse
Erener S, Ellis CE, Ramzy A Et al. Deletion of pancreas-specific miR-216a reduces beta-cell mass and inhibits pancreatic cancer progression in mice Cell reports. Medicine Nov 16 2021 [PMID: 34841287]

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Blogs on Synaptophysin.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

Synaptophysin a Marker Protein in Neuroendocrine Cells
Synaptophysin a Marker Protein in Neuroendocrine Cells Synaptophysin is a major integral membrane glycoprotein of neuronal synaptic vesicles present in virtually all synapses and shows a high degree of evolutionary conservation across the mammals. Syn...  Read full blog post.

Characterizing Synaptophysin is "a Snap"
Synaptophysin is an integral membrane glycoprotein found within the small synaptic vesicles in brain and endocrine cells. Studies with synaptophysin antibodies show that it is one of the most abundant small vesicle proteins, constituting approximately...  Read full blog post.

Synaptophysin and Dementing Disorders
 Synaptophysin (a presynaptic vesicle protein) is an integral membrane glycoprotein originally isolated from presynaptic vesicles of bovine neurons. Synaptophysin is found in all nerve terminals and synaptophysin measurements have been used to quantif...  Read full blog post.

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Gene Symbol SYP