Synaptophysin Antibody

Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.

Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain dilution 1:100

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:00 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Rat brain-Synaptophysin.

Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Mouse Brain- Synaptophysin.

• Reactivity: Hu, Mu, Rt, Ch, Fe, Pm
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Synaptophysin Antibody Summary

• Immunogen: Synthetic peptide taken within amino acid region 1-100 on human Synaptophysin protein.
• Localization: Integral membrane protein. Synaptic vesicles.
• Marker: Pre-synaptic marker
• Specificity: This antibody does not cross-react with other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:125
  • Western Blot 1:500-1:1000
• Application Notes: Immunofluorescence reactivity reported in (PMID: 21368026). Immunoprecipitation reactivity reported in (PMID: 22970294). Synaptophysin antibody validated for IP, IHC-Frozen from verified customer reviews.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Feline, Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Synaptophysin Antibody

• Major synaptic vesicle protein p38
• MRX
• MRXSYP
• Synaptophysin
• SYP

Background

Synaptophysin is a 38 kDa synaptic vesicle associated protein. The pathology of synaptic components is investigated by measuring the expression of synaptophysin that is mainly concentrated in synaptic terminals. The expression of synaptophysin is a measure of dynamic plastic changes in nerve tissue. A decrease in synaptophysin and other synaptic vesicle associated proteins (Synaptotagmin, synaptosomal associated protein 25 kDa, SNAP-25, CLC ad GAP-43) generally means degeneration in the nerve fibers and an increased expression results in enhanced neuroplasticity and neuronal hypertrophy. In conditions involving generative changes in the nerve tissue, the expression of psynaptophysin is generally reduced, for example, in Schizophrenia an Alzhiemers disease. A decreased expression of synaptophysin in hippocampus of Schizoprenic patients has also been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Synaptophysin Antibody (NB300-653)(9)

Publications using NB300-653

• Kumar S, Orlov E, Gowda P et al. Synaptosome microRNAs regulate synapse functions in Alzheimer's disease NPJ genomic medicine 2022-08-08 [PMID: 35941185] (WB, Mouse)
• Sinopoulou E, Rosenzweig ES, Conner JM et al. Rhesus macaque versus rat divergence in the corticospinal projectome Neuron 2022-07-31 [PMID: 35917818]
• Sui L, Danzl N, Campbell SR et al. Beta Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells Diabetes 2017-09-20 [PMID: 28931519] (Human)
• Lam CS, Tipoe GL, Wong JK et al. M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus. PLoS ONE. 2016-11-21 [PMID: 27870896] (WB, Rat)
• D'Cruz TS, Weibley BN, Kimball SR, Barber AJ. Post-Translational Processing of Synaptophysin in the Rat Retina Is Disrupted by Diabetes PLoS One 2012-01-01 [PMID: 22970294] (IP, Rat)
• Postupna N, Rose SE, Bird TD et al. Novel antibody capture assay for paraffin-embedded tissue detects wide-ranging amyloid Beta and paired helical filament-tau accumulation in cognitively normal older adults Brain Pathol 2012-07-01 [PMID: 21999410] (Human)
• Dash PK, Gorantla S, Gendelman HE, et al. Loss of Neuronal Integrity during Progressive HIV-1 Infection of Humanized Mice. J Neurosci. [PMID: 21368026] (ICC/IF, Mouse)
• Kilinc M, Creson TK, Rojas C et al. Splicing of the SynGAP Carboxyl-Terminus Enables Isoform-Specific Tuning of NMDA Receptor Signaling Linked to Cognitive Function bioRxiv 2020-01-01 (WB, Mouse)
• Erener S, Ellis CE, Ramzy A Et al. Deletion of pancreas-specific miR-216a reduces beta-cell mass and inhibits pancreatic cancer progression in mice Cell reports. Medicine 2021-11-16 [PMID: 34841287]

Reviews for Synaptophysin Antibody (NB300-653) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Rat, Feline.

reviewed by: Kazuya Oikawa IHC-Fr Feline 09/15/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Retina
• Species: Feline
• Lot: Pld100lg

reviewed by: Verified Customer IP Rat 08/16/2010

Summary

• Application: Immunoprecipitation
• Sample Tested: Rat Retinal Tissue
• Species: Rat

Bioinformatics

• Gene Symbol: SYP
• Entrez:
  • 6855(Human)
  • 20977(Mouse)
  • 24804(Rat)
• Uniprot:
  • P08247()