Calbindin D-28K Antibody

Images

 
Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, NBP2-50028, dilution 1:5,000 in green: [1] protein standard ...read more
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Cultured mouse embryonic kidney stained with Calbindin antibody (1:200) and DAPI. Image from verified customer review.
Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028] - Representative images of horizontal cells (Calbindin D-28K, cyan) in wild type control and C1q-/- mice at P8, P13, P17, and P23. Horizontal cells in C1q-/- ...read more
Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat brain lysate (left), 0.5 ug of His-tagged recombinant proteins (right) were probed with NBP2-50028 at 1:5,000. Lane 1: Parvalbumin, Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Adult rat brain cortex (Left) and striatum (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained ...read more
Immunohistochemistry Free-Floating: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat cerebellum section stained with chicken Calbindin D-28K pAb, dilution 1:2,000 (Green), and costained with rabbit MeCP2 pAb, ...read more
Amb optogenetic stimulation in Calb1cre or Ghsrcre mice results in apnea mediated by non-cholinergic neurons.Data are from same stimulation trials as Fig. 4. e) Immunostaining of ACP neurons in Amb in a Calb1cre mouse ...read more
Phd2 deletion leads to activation of the Akt–mTOR pathway. a RPPA analysis of tumors with homozygous deletion of Phd2. Tumor tissues from Tyr::CreER; BRafV600E; Phd2−/− or Tyr::CreER; BRafV600E mice were processed ...read more
UDM1 is required for RNF168-mediated 53BP1 recruitment.a Schematic illustration of RNF8 and RNF168 structural domain organization, RNF168 fragments, and chimeric proteins used as in b and c. FHA–forkhead-association ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

Calbindin D-28K Antibody Summary

Immunogen
Full-length recombinant human Calbindin D-28K. [UniProt# P05937]
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
CALB1
Purity
IgY purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 - 1:5000
Application Notes
In Western blot, this antibody recognizes a clear band at ~28 kDa in rat brain lysates which represents Calbindin and it reacts only with Calbindin protein, and not other calcium-binding proteins. A band maybe seen at ~60 kDa and is most likely the dimer of Calbindin. ICC/IF reported from a verified customer review. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 29208685).
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-50028 in the following applications:

Publications
Read Publications using
NBP2-50028 in the following applications:

Reactivity Notes

Mouse reported from a verifed customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Supplied as an aliquot of IgY preparation
Preservative
5mM Sodium Azide
Purity
IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Calbindin D-28K Antibody

  • CAB27
  • CALB
  • calbindin 1, (28kD)
  • calbindin 1, 28kDa
  • Calbindin D28
  • calbindin
  • D-28K
  • RTVL-H protein
  • Vitamin D-dependent calcium-binding protein, avian-type

Background

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calbindin D-28K Antibody (NBP2-50028)(11)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, IHC-WhMt.


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ICC/IF
(1)
IF/IHC
(5)
IHC-Fr
(4)
IHC-P
(1)
IHC-WhMt
(1)
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Mouse
(8)
Rat
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-50028 Applications Species
Chen TI, Hsu PC, Lee NC et al. Loss of Flot2 expression in deep cerebellar nuclei neurons of mice with Niemann-Pick disease type C Heliyon 2023-07-01 [PMID: 37539272] (IHC-Fr, Mouse)

Details:
1:1000 dilution
IHC-Fr Mouse
Jiang D, Burger CA, Akhanov V et al. Neuronal signal-regulatory protein alpha drives microglial phagocytosis by limiting microglial interaction with CD47 in the retina Immunity 2022-11-09 [PMID: 36379210] (IHC-Fr, Mouse) IHC-Fr Mouse
Romano LEL, Aw WY, Hixson KM et al. Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization Cell reports 2022-11-01 [PMID: 36323248]
Liang JH, Alevy J, Akhanov V et al. Kctd7 deficiency induces myoclonic seizures associated with Purkinje cell death and microvascular defects Disease models & mechanisms 2022-08-16 [PMID: 35972048] (IHC-P, Mouse) IHC-P Mouse
Kwon KM, Pak JH, Jeon CJ Immunocytochemical localization of the AMPA glutamate receptor subtype GluR2/3 in the squid optic lobe Acta histochemica 2022-08-10 [PMID: 35963117] (IF/IHC)

Details:
Pacific flying squids (Tordarodes pacificus), dilution used for IHC 1:500
IF/IHC
Veerakumar A, Yung AR, Liu Y, Krasnow MA Molecularly defined circuits for cardiovascular and cardiopulmonary control Nature 2022-06-01 [PMID: 35650438] (IF/IHC, Mouse) IF/IHC Mouse
Garcia H, Serafin AS, Silbermann F et al. Agonists of prostaglandin E2 receptors as potential first in class treatment for nephronophthisis and related ciliopathies Proceedings of the National Academy of Sciences of the United States of America 2022-05-03 [PMID: 35482924] (IF/IHC, Mouse) IF/IHC Mouse
D'Souza SP, Swygart DI, Wienbar SR Et al. Retinal patterns and the cellular repertoire of neuropsin (Opn5) retinal ganglion cells The Journal of comparative neurology 2021-11-07 [PMID: 34743323] (IHC-WhMt, Mouse) IHC-WhMt Mouse
Burger, C A, Jiang, D Et al. C1q Regulates Horizontal Cell Neurite Confinement in the Outer Retina. Front Neural Circuits 2020-11-13 [PMID: 33177995] (IHC-Fr, ICC/IF, Rat) IHC-Fr, ICC/IF Rat
Burger CA, Alevy J, Casasent AK et al. LKB1 coordinates neurite remodeling to drive synapse layer emergence in the outer retina Elife 2020-05-07 [PMID: 32378514] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 11 Publications.

Review for Calbindin D-28K Antibody (NBP2-50028) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-50028:
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ICC
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Calbindin D-28K NBP2-50028
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5
reviewed by:
Julia Tarnick
ICC Mouse 08/04/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedEmbryonic kidney
SpeciesMouse
Lot7363-052615

Comments

CommentsFixation: 4% PFA, 30min RT
Permeabilization: 100 % methanol -20°C, 20 min
blocking buffer: 3% donkey serum
antibody dilution: 1:200 in blocking buffer, incubation overnight 4°C

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calbindin D-28K Antibody (NBP2-50028). (Showing 1 - 1 of 1 FAQs).

  1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
    • This link will take you to all of our alpha7 nicotinic cholingergic receptor antibodies currently available for purchase. Please see the following link for our Calbinin D28K antibodies reactive against mouse and human. We currently do not have any Parvalbumin antibodies that have been tested in mouse. I would like to introduce you to our Innovators Reward Program. Try our primary antibodies in an untested species or application, without the financial risk of failure. The pricing and availability of our products depends on your country.

Secondary Antibodies

 

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Recent Reviews

5
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1
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Julia Tarnick
08/04/2018
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol CALB1
Uniprot