Calbindin D-28K Antibody

Images

 
Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, NBP2-50028, dilution 1:5,000 in green: [1] protein standard ...read more
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Cultured mouse embryonic kidney stained with Calbindin antibody (1:200) and DAPI. Image from verified customer review.
Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat brain lysate (left), 0.5 ug of His-tagged recombinant proteins (right) were probed with NBP2-50028 at 1:5,000. Lane 1: Parvalbumin, Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Adult rat brain cortex (Left) and striatum (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
10 mg/ml

Order Details

Calbindin D-28K Antibody Summary

Immunogen
Full-length recombinant human Calbindin D-28K. [UniProt# P05937]
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
CALB1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:1000 - 1:5000
Application Notes
In Western blot, this antibody recognizes a clear band at ~30 kDa in rat brain lysates which represents Calbindin and it reacts only with Calbindin protein, and not other calcium-binding proteins. A band maybe seen at ~60 kDa and is most likely the dimer of Calbindin.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-50028 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
10 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Calbindin D-28K Antibody

  • CAB27
  • CALB
  • calbindin 1, (28kD)
  • calbindin 1, 28kDa
  • Calbindin D28
  • calbindin
  • D-28K
  • RTVL-H protein
  • Vitamin D-dependent calcium-binding protein, avian-type

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calbindin D-28K Antibody (NBP2-50028) (0)

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Review for Calbindin D-28K Antibody (NBP2-50028) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-50028:
Filter by Applications
ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Calbindin D-28K NBP2-50028
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reviewed by:
Julia Tarnick
ICC Mouse 08/04/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedEmbryonic kidney
SpeciesMouse
Lot7363-052615

Comments

CommentsFixation: 4% PFA, 30min RT
Permeabilization: 100 % methanol -20°C, 20 min
blocking buffer: 3% donkey serum
antibody dilution: 1:200 in blocking buffer, incubation overnight 4°C

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calbindin D-28K Antibody (NBP2-50028). (Showing 1 - 1 of 1 FAQ).

  1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
    • This link will take you to all of our <a href="http://www.novusbio.com/productsearch/alpha7%2Bnicotinic%2Bcholinergic%2Breceptor%2B#fq=common_name%3A%22CHRFAM7A%22&fq=species%3A%22Mouse%22" target="_self">alpha7 nicotinic cholingergic receptor antibodies</a> currently available for purchase. Please see the following link for our <a href="http://www.novusbio.com/CALB1-Calbindin-D-28K-Antibody-EP3478_NBP1-95327.html" target="_self">Calbinin D28K</a> antibodies reactive against mouse and human. We currently do not have any <a href="http://www.novusbio.com/productsearch/parvalbumin#fq=common_name%3A%22Parvalbumin%22" target="_self">Parvalbumin</a> antibodies that have been tested in mouse. I would like to introduce you to our <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a>. Try our primary antibodies in an untested species or application, without the financial risk of failure. The pricing and availability of our products depends on your country.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
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0
2
0
1
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Julia Tarnick
08/04/2018
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol CALB1
Uniprot