Carbonic Anhydrase III/CA3 Antibody [Unconjugated]

Western blot shows lysates of human, mouse, and rat skeletal muscle tissue and heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/ Rat Carbonic Anhydrase III/CA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2185) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Carbonic Anhydrase III/CA3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase III/CA3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Carbonic Anhydrase III/CA3
  Ala2-Lys260
  Accession # P07451
• Specificity: Detects human Carbonic Anhydrase III/CA3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CA3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase III/CA3 Antibody [Unconjugated]

• CA3
• CAIII
• CA-III
• Car3
• Carbonate dehydratase III
• carbonic anhydrase 3
• Carbonic Anhydrase III
• carbonic anhydrase III, muscle specific
• EC 4.2.1.1

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1).

Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50‑60% of adult levels at birth (2).

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carbonic Anhydrase III/CA3 Antibody (AF2185)(4)

Publications using AF2185

• CH Murphy, M Shankaran, TA Churchward, CJ Mitchell, NM Kolar, LM Burke, JA Hawley, A Kassis, LG Karagounis, K Li, C King, M Hellerstei, SM Phillips Effect of resistance training and protein intake pattern on myofibrillar protein synthesis and proteome kinetics in older men in energy restriction J. Physiol. (Lond.), 2018-04-17;0(0):. 2018-04-17 [PMID: 29532476] (Immunoprecipitation, Human)
• Mahalakshmi Shankaran, Chelsea L. King, Thomas E. Angel, William E. Holmes, Kelvin W. Li, Marc Colangelo et al. Circulating protein synthesis rates reveal skeletal muscle proteome dynamics Journal of Clinical Investigation 12/14/2015 [PMID: 26657858]
• Mahalakshmi Shankaran, Chelsea L. King, Thomas E. Angel, William E. Holmes, Kelvin W. Li, Marc Colangelo et al. Circulating protein synthesis rates reveal skeletal muscle proteome dynamics Journal of Clinical Investigation 2015-12-14 [PMID: 26657858] (Immunoprecipitation, Human, Rat)
• Matsumoto N, Ezaki J, Komatsu M, Takahashi K, Mineki R, Taka H, Kikkawa M, Fujimura T, Takeda-Ezaki M, Ueno T, Tanaka K, Kominami E Comprehensive proteomics analysis of autophagy-deficient mouse liver. Biochem. Biophys. Res. Commun., 2008-02-04;368(3):643-9. 2008-02-04 [PMID: 18249191] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CA3
• Uniprot:
  • P07451()