Prolactin Antibody [Biotin]

Prolactin was detected in immersion fixed paraffin-embedded sections of mouse pituitary using Goat Anti-Mouse Prolactin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1445) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Prolactin Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse Prolactin
  Leu32-Cys228
  Accession # NP_035294
• Specificity: Detects mouse Prolactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Prolactin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRL
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture:Mouse Prolactin Antibody (Catalog # AF1445)
  ELISA Detection: Mouse Prolactin Biotinylated Antibody (Catalog # BAF1445)
  Standard: Recombinant Mouse Prolactin (Catalog # 1445-PL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prolactin Antibody [Biotin]

• PRL
• Prolactin

Background

Prolactin (PRL) is a neuroendocrine pituitary hormone. Prolactin is synthesized by the anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, NK cells and breast cancer cells. Originally characterized as a lactogenic hormone, further studies have demonstrated broader roles in breast cancer development, regulation of reproductive function, and immunoregulation. In the immune system, Prolactin has been shown to be secreted by human PBMC and to act as a proliferative growth factor. Additionally, Prolactin treatment of human PBMC has been shown to enhance IFN-gamma production. In the breast, Prolactin-induced morphogenesis of the mammary cells is mediated through IGF-2, which in turn upregulates cyclin D1. Prolactin has several molecular forms. The predominant form is a monomer; the non-glycosylated form is 23 kDa and the glycosylated form is 25 kDa. Glycosylated Prolactin is removed from the circulation faster and has been reported to have lower biological potency. Mouse Prolactin cDNA encodes a 228 amino acid (aa) residue protein with a putative 31 aa residue signal peptide. The Prolactin receptor is a transmembrane type I glycoprotein that belongs to the cytokine hematopoietic receptor family. B cells, T cells, macrophages, NK cells, monocytes, CD34⁺ progenitor cells, neutrophils, mammary gland, liver, kidney, adrenals, ovaries, testis, prostrate, seminal vesicles, and hypothalamus have all been shown to express the Prolactin receptor. Three forms of the receptor, generated by differential splicing, have been identified. These isoforms differ in the length of their cytoplasmic domains. It is believed that the short cytoplasmic form is non-functional. Prolactin signal transduction involves the JAK/STAT families and Src kinase family (1‑9).

1. Freeman, M. et al. (2000) Physiological Reviews 80:1523.
2. Ben-Johnson, N. et al. (1996) Endoc. Rev. 17:639.
3. Cesario, T. et al. (1994) Proc. Soc. Exp. Biol. Med. 205:89.
4. Price, A.E. et al. (1995) Endoc. 136:4827.
5. Hoffmann, T. et al. (1993) J. Endoc. Invest. 16:807.
6. Cole, E. et al. (1991) Endoc. 129:2639.
7. Lewis, U. et al. (1985) Endoc. 116:359.
8. Matalk, K. (2003) Cytokine 21:187.
9. Brisken, C. et al. (2002) Dev. Cell 3:877.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prolactin Antibody (BAF1445)(2)

Publications using BAF1445

• Alice S Kaanta, Carl Virtanen, Laura M Selfors, Joan S Brugge, Benjamin G Neel Evidence for a multipotent mammary progenitor with pregnancy-specific activity Breast Cancer Research 2013-08-15 [PMID: 23947835] (Flow Cytometry, Mouse)
• Ledesma-Soto Y, Blanco-Favela F, Fuentes-Panana EM, Tesoro-Cruz E, Hernandez-Gonzalez R, Arriaga-Pizano L, Legorreta-Haquet MV, Montoya-Diaz E, Chavez-Sanchez L, Castro-Mussot ME, Chavez-Rueda AK Increased levels of prolactin receptor expression correlate with the early onset of lupus symptoms and increased numbers of transitional-1 B cells after prolactin treatment. BMC Immunol., 2012-03-09;13(0):11. 2012-03-09 [PMID: 22404893] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: PRL
• Uniprot:
  • NP_035294()