STAT5b Antibody [Unconjugated]

Images

 
Western Blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line and M1 mouse myeloid leukemia cell line. ...read more
STAT5b was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit Anti-Human/Mouse STAT5b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1584) at 5 µg/mL for 3 hours at room ...read more
Biological Strategies: HeLa human cervical epithelial carcinoma and M1 mouse myeloid leukemia cell line were untreated (-) or activated (+) with 100 ng/mL Recombinant Human IFN-gamma (285-IF) for 15 minutes. ...read more
Jurkat human acute T cell leukemia cell line was stained with Rabbit Anti-Human/Mouse STAT5b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1584, filled histogram) or control antibody (AB-105-C, open ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5b at ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT5b knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Rabbit ...read more
Genetic Strategies: Western blot shows lysates of HAP1 human near-haploid cell line and STAT5b knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse STAT5b ...read more
Immunoprecipitation was performed on cell lysate of HAP1 human near-haploid cell line using 2.0 μg of Rabbit Anti-Human Stat5b Polyclonal Antibody (Catalog # AF1584) pre-coupled to protein G or protein A beads. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IP, CyTOF-ready, Flow, ICC/IF, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

STAT5b Antibody [Unconjugated] Summary

Immunogen
Human STAT5b synthetic peptide
aa 777-787
Specificity
Detects human and mouse STAT5b.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STAT5B
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Immunoprecipitation 1-5 ug/500 ug cell lysate
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 0.2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 4
using
AF1584 in the following applications:

Publications
Read Publications using
AF1584 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT5b Antibody [Unconjugated]

  • signal transducer and activator of transcription 5B
  • STAT5
  • STAT5b
  • transcription factor STAT5B

Background

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT5b Antibody (AF1584)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
Immunoprecipitation
(2)
Western Blot
(2)
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Filter By Species
Human
(2)
Mouse
(4)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF1584 Applications Species
Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse) IHC Mouse
Alexander Vargas-Hernández, Agnieszka Witalisz-Siepracka, Michaela Prchal-Murphy, Klara Klein, Sanjana Mahapatra, Waleed Al-Herz et al. Human signal transducer and activator of transcription 5b (STAT5b) mutation causes dysregulated human natural killer cell maturation and impaired lytic function Journal of Allergy and Clinical Immunology 2020-01-01 [PMID: 31600547] (Western Blot, Human) Western Blot Human
Jimena Trillo-Tinoco, Rosa A. Sierra, Eslam Mohamed, Yu Cao, Álvaro de Mingo-Pulido, Danielle L. Gilvary et al. AMPK Alpha-1 Intrinsically Regulates the Function and Differentiation of Tumor Myeloid-Derived Suppressor Cells Cancer Research 2019-10-01 [PMID: 31409640]
Daniella M. Schwartz, Taylor K. Farley, Nathan Richoz, Chen Yao, Han-Yu Shih, Franziska Petermann et al. Retinoic Acid Receptor Alpha Represses a Th9 Transcriptional and Epigenomic Program to Reduce Allergic Pathology Immunity 2019-01-01 [PMID: 30650370]
Yrina Rochman, Krista Dienger-Stambaugh, Phoebe K. Richgels, Ian P. Lewkowich, Andrey V. Kartashov, Artem Barski et al. TSLP signaling in CD4 + T cells programs a pathogenic T helper 2 cell state Science Signaling 2018-03-15 [PMID: 29535264]
JX Lin, N Du, P Li, M Kazemian, T Gebregiorg, R Spolski, WJ Leonard Critical functions for STAT5 tetramers in the maturation and survival of natural killer cells Nat Commun, 2017-11-06;8(1):1320. 2017-11-06 [PMID: 29105654] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Schaller-Schonitz M, Barzan D, Williamson A, Griffiths J, Dallmann I, Battmer K, Ganser A, Whetton A, Scherr M, Eder M BCR-ABL affects STAT5A and STAT5B differentially. PLoS ONE, 2014-05-16;9(5):e97243. 2014-05-16 [PMID: 24836440] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Bao YC, Nomura Y, Moon Y, Tonozuka Y, Minoshima Y, Hatori T, Tsuchiya A, Kiyono M, Nosaka T, Nakajima H Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors. J. Cell Biol., 2006-12-18;175(6):937-46. 2006-12-18 [PMID: 17178910] (ICC, Western Blot, Human, Mouse) ICC, Western Blot Human, Mouse

Review for STAT5b Antibody (AF1584) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1584:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Human 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22729867
SpeciesHuman

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Additional STAT5b Products

Blogs on STAT5b.

Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.

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Verified Customer
01/05/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol STAT5B