SHBG Antibody

SHBG was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human SHBG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2656) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SHBG Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SHBG
  Leu30-His402
  Accession # P04278
• Specificity: Detects human SHBG in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SHBG
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHBG Antibody

• ABP
• ABPTestosterone-estrogen-binding globulin
• androgen binding protein
• MGC126834
• MGC138391
• SBP
• sex hormone-binding globulin
• Sex steroid-binding protein
• SHBG
• TEBG
• Testis-specific androgen-binding protein
• testosterone-binding beta-globulin
• Testosterone-estradiol-binding globulin

Background

Human SHBG (also known as ABP or androgen-binding protein) is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 amino acid (aa) long laminin alpha chain G-like domains. Each SHBG monomer is a 47-53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHBG Antibody (AF2656)(2)

Publications using AF2656

• E Blommaert, R Péanne, NA Cherepanov, D Rymen, F Staels, J Jaeken, V Race, L Keldermans, E Souche, A Corveleyn, R Sparkes, K Bhattachar, C Devalck, R Schrijvers, F Foulquier, R Gilmore, G Matthijs Mutations in MAGT1 lead to a glycosylation disorder with a variable phenotype Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):. 2019 [PMID: 31036665] (Immunoprecipitation, Human)
• Cherepanova N, Shrimal S, Gilmore R Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins. J Cell Biol, 0;206(4):525-39. 0 [PMID: 25135935] (Western Blot, Human)

Review for SHBG Antibody (AF2656) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous IHC-P Human 04/04/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Liver cancer tissue
• Species: Human
• Lot: VFJ021612A

Bioinformatics

• Gene Symbol: SHBG
• Uniprot:
  • P04278()