Prolactin: Novus Biologicals

AF1445

Prolactin Antibody [Unconjugated]

100 μg

AF1445

HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse pituitary tissue and rat pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse pituitary tissue and rat pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

1445-PL) stimulates proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Prolactin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445). The ND₅₀ is typically 0.25-1.0 µg/mL." class="big_lightbox">

1445-PL) stimulates proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Prolactin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445). The ND₅₀ is typically 0.25-1.0 µg/mL." class="big_thumb" />

VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lactotroph cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Prolactin was detected in immersion fixed paraffin-embedded sections of Mouse Pituitary using Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # <a class=

VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lactotroph cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Simple Western shows lysates of mouse pituitary tissue and rat pituitary tissue, loaded at 0.2 mg/ml. A specific band was detected for Prolactin at approximately 26 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # af1445). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Overexpression of ER alpha rescues miR-130a-3p-inhibited expression of PRL. The GH3 cells were transfected with the miR-130a-3p mimic and the ER alpha overexpressing vector. The rescue efficiency of ER alpha in the GH3 cells was confirmed by western blotting. (A) The protein expression level of ER alpha was detected by western blotting. GAPDH was used as loading control. (B) The protein expression level of PRL was detected by western blotting. GAPDH was used as loading control. (C) Quantitation of ER alpha protein level. Data are presented as mean ± S.E.M of n = 4 samples per group (P < 0.05 by ANOVA). (D) Quantitation of PRL protein level. Data are presented as mean ± S.E.M of n = 4 samples per group (P < 0.05 by ANOVA). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32194503), licensed under a CC-BY license. Not internally tested by R&D Systems.

MiR-130a-3p overexpression reduces PRL expression in GH3 cells. GH3 cells were transfected with miR-130a-3p mimic and NC and then the expressions of miR-130a-3p and PRL were analyzed. (A) The expression level of miR-130a-3p was detected by quantitative real-time PCR (qRT-PCR). U6 snRNA was used to normalize the miRNA expression. Data are presented as mean ± S.E.M of n = 6 samples per group (*P < 0.05 by t-test). (B) The expression level of PRL mRNA was detected by qRT-PCR. GAPDH was used to normalize each gene expression. Data are presented as mean ± S.E.M of n = 6 samples per group (*P < 0.05 by t-test). (C) The protein level of PRL in GH3 cells was analyzed by western blotting. GAPDH was used as loading control. (D) Quantitation of the PRL protein level. Data are presented as mean ± S.E.M of n = 4 samples per group (*P < 0.05 by t-test). (E) The protein level of PRL was detected by ICC after GH3 cells transfected with miR-130a-3p mimic and NC. Scale bar, 50 μM. **P < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32194503), licensed under a CC-BY license. Not internally tested by R&D Systems.

Heat stress increases miR-130a-3p expression, reduces PRL and ER alpha expressions in GH3 cells. GH3 cells were separately cultured in 37 or 41°C for 24 h, after that, miR-130a-3p, PRL, and ER alpha expressions were analyzed. (A) The expression level of miR-130a-3p was detected by quantitative real-time PCR (qRT-PCR). U6 snRNA was used to normalize the miRNA expression. Data are presented as mean ± S.E.M of n = 4 samples per group (*P < 0.05 by t-test). (B) The expression levels of HSP70, ER alpha , and PRL mRNAs in GH3 cells were detected by qRT-PCR. GAPDH was used to normalize each gene expression. Data are presented as mean ± S.E.M of n = 4 samples per group (*P < 0.05, ns, not significant by t-test). (C) The protein levels of HSP70, ER alpha , and PRL in GH3 cells were analyzed by western blotting. GAPDH was used as loading control. (D) Quantitation of HSP70, ER alpha , and PRL protein levels. Data are presented as mean ± S.E.M of n = 4 samples per group (*P < 0.05 by t-test). **P < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32194503), licensed under a CC-BY license. Not internally tested by R&D Systems.

Heat stress reduces PRL and ER alpha expressions as well as increases miR-130a-3p expression in the pituitary gland. The mice of treated groups were placed in 40°C for 2 h each time, and the stimulus, respectively lasted 1 and 7 days. The mice in control group were fed as normal in 25°C. Then the expressions of PRL and ER alpha in the pituitary gland were analyzed. (A–B) The mRNA levels of PRL (A) and ER alpha (B) in the pituitary gland of three groups were analyzed by quantitative real-time PCR (qRT-PCR). GAPDH was used to normalize each gene expression. Data are presented as mean ± S.E.M of n = 5 animals per group. Bars that share different letter are significantly different (P < 0.05 by ANOVA). (C) The serum PRL concentration was detected by Elisa assay. Data are presented as mean ± S.E.M of n = 5 animals per group. Bars that share different letter are significantly different (P < 0.05 by ANOVA). (D) The protein levels of PRL and ER alpha in the pituitary gland were analyzed by western blotting. beta -actin was used as loading control. (E) Quantitation of PRL and ER alpha protein levels. Data are presented as mean ± SD of n = 3 animals per group. Bars that share different letter are significantly different (P < 0.05 by ANOVA). The expression levels of miR-130a-3p (F), miR-130b-3p (G), miR-301a-3p (H), and miR-301b-3p (I) were detected by qRT-PCR. U6 snRNA was used to normalize each miRNA expression. Data are presented as mean ± S.E.M of n = 5 animals per group. Bars that share different letter are significantly different (P < 0.05 by ANOVA). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32194503), licensed under a CC-BY license. Not internally tested by R&D Systems.

1445-PL) was serially diluted and captured by Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse Prolactin (Catalog # <a class=

1445-PL) was serially diluted and captured by Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1445-PL) was serially diluted and captured by Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

1445-PL) was serially diluted and captured by Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1445U-100UG
Alexa Fluor 405	AF1445V-100UG
Alexa Fluor 488	AF1445G-100UG
Alexa Fluor 532	AF1445X-100UG
Alexa Fluor 594	AF1445T-100UG
Alexa Fluor 647	AF1445R-100UG
Alexa Fluor 700	AF1445N-100UG
Alexa Fluor 750	AF1445S-100UG
Alexa Fluor Plus 405	AF1445AFP405-100UG
Alexa Fluor Plus 488	AF1445AFP488-100UG
Alexa Fluor Plus 555	AF1445AFP555-100UG
Alexa Fluor Plus 594	AF1445AFP594-100UG
Alexa Fluor Plus 647	AF1445AFP647-100UG
Alexa Fluor Plus 680	AF1445AFP680-100UG
Allophycocyanin	AF1445A
Biotin	BAF1445
CoraFluor 1	AF1445CL1
DyLight 350	AF1445D
DyLight 405	AF1445E
DyLight 488	AF1445K
DyLight 550	AF1445L
DyLight 594	AF1445M
DyLight 650	AF1445W
DyLight 680	AF1445Y
DyLight 755	AF1445Z
FITC	AF1445F
HRP	AF1445H
Janelia Fluor 525	AF1445JF525
Janelia Fluor 549	AF1445I
Janelia Fluor 646	AF1445J
Janelia Fluor 669	AF1445JF669
PE	AF1445P
PerCP	AF1445C
mFluor Violet 450 SE	AF1445MFV450
mFluor Violet 500 SE	AF1445MFV500
mFluor Violet 610 SE	AF1445MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1445-SP

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AF682

Prolactin Antibody [Unconjugated]

(7 Publications)

100 μg

AF682

HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of sperm cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Prolactin was detected in immersion fixed paraffin-embedded sections of human testis using Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of sperm cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western shows lysates of human pituitary, loaded at 0.2 mg/ml. A specific band was detected for Prolactin at approximately 30 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

682-PL) induced proliferation in the Nb2 11 rat lymphoma cell line. The Neutralization Dose (ND₅₀) for this effect is typically 0.0200-0.200 µg/mL." class="big_lightbox">

Human Prolactin Antibody (Catalog # AF682) neutralizes Recombinant Human Prolactin (<a class=

682-PL) induced proliferation in the Nb2 11 rat lymphoma cell line. The Neutralization Dose (ND₅₀) for this effect is typically 0.0200-0.200 µg/mL." class="big_thumb" />

682-PL) was serially diluted and captured by Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Prolactin (Catalog # <a class=

682-PL) was serially diluted and captured by Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

682-PL) was serially diluted and captured by Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

682-PL) was serially diluted and captured by Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF682U-100UG
Alexa Fluor 405	AF682V-100UG
Alexa Fluor 488	AF682G-100UG
Alexa Fluor 532	AF682X-100UG
Alexa Fluor 594	AF682T-100UG
Alexa Fluor 647	AF682R-100UG
Alexa Fluor 700	AF682N-100UG
Alexa Fluor 750	AF682S-100UG
Alexa Fluor Plus 405	AF682AFP405-100UG
Alexa Fluor Plus 488	AF682AFP488-100UG
Alexa Fluor Plus 555	AF682AFP555-100UG
Alexa Fluor Plus 594	AF682AFP594-100UG
Alexa Fluor Plus 647	AF682AFP647-100UG
Alexa Fluor Plus 680	AF682AFP680-100UG
Allophycocyanin	AF682A
Biotin	BAF682
CoraFluor 1	AF682CL1
DyLight 350	AF682D
DyLight 405	AF682E
DyLight 488	AF682K
DyLight 550	AF682L
DyLight 594	AF682M
DyLight 650	AF682W
DyLight 680	AF682Y
DyLight 755	AF682Z
FITC	AF682F
HRP	AF682H
Janelia Fluor 525	AF682JF525
Janelia Fluor 549	AF682I
Janelia Fluor 646	AF682J
Janelia Fluor 669	AF682JF669
PE	AF682P
PerCP	AF682C
mFluor Violet 450 SE	AF682MFV450
mFluor Violet 500 SE	AF682MFV500
mFluor Violet 610 SE	AF682MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF682-SP

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NBP2-02142

Prolactin Antibody (OTI6B1)

(1 Publication)

0.1 ml

NBP2-02142

Immunohistochemistry-Paraffin: Prolactin Antibody (6B1) [NBP2-02142] - Staining of paraffin-embedded Human Ovary tissue using anti-Prolactin mouse monoclonal antibody.

Flow Cytometry: Prolactin Antibody (6B1) [NBP2-02142] - Analysis of Jurkat cells, using anti-Prolactin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunocytochemistry/Immunofluorescence: Prolactin Antibody (6B1) [NBP2-02142] - Immunofluorescent staining of HepG2 cells using anti-Prolactin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Prolactin Antibody (6B1) [NBP2-02142] - Staining of paraffin-embedded Human breast tissue using anti-Prolactin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Prolactin Antibody (6B1) [NBP2-02142] - Staining of paraffin-embedded Human endometrium tissue using anti-Prolactin mouse monoclonal antibody.

Flow Cytometry: Prolactin Antibody (6B1) [NBP2-02142] - Analysis of Hela cells, using anti-Prolactin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73609AF350
Alexa Fluor 405	NBP2-73609AF405
Alexa Fluor 488	NBP2-73609AF488
Alexa Fluor 532	NBP2-73609AF532
Alexa Fluor 594	NBP2-73609AF594
Alexa Fluor 647	NBP2-73609AF647
Alexa Fluor 700	NBP2-73609AF700
Alexa Fluor 750	NBP2-73609AF750
Alexa Fluor Plus 405	NBP2-73609AFP405
Alexa Fluor Plus 488	NBP2-73609AFP488
Alexa Fluor Plus 555	NBP2-73609AFP555
Alexa Fluor Plus 594	NBP2-73609AFP594
Alexa Fluor Plus 647	NBP2-73609AFP647
Alexa Fluor Plus 680	NBP2-73609AFP680
Allophycocyanin	NBP2-73609APC
Allophycocyanin/Cy7	NBP2-73609APCCY7
Biotin	NBP2-73609B
CoraFluor 1	NBP2-73609CL1
DyLight 350	NBP2-73609UV
DyLight 405	NBP2-73609V
DyLight 488	NBP2-73609G
DyLight 550	NBP2-73609R
DyLight 594	NBP2-73609DL594
DyLight 650	NBP2-73609C
DyLight 680	NBP2-73609FR
DyLight 755	NBP2-73609IR
FITC	NBP2-73609F
HRP	NBP2-73609H
Janelia Fluor 525	NBP2-73609JF525
Janelia Fluor 549	NBP2-73609JF549
Janelia Fluor 646	NBP2-73609JF646
Janelia Fluor 669	NBP2-73609JF669
PE	NBP2-73609PE
PE/Atto594	NBP2-73609PEATT594
PE/Cy5.5	NBP2-73609PECY55
PE/Cy7	NBP2-73609PECY7
PerCP	NBP2-73609PCP
mFluor Violet 450 SE	NBP2-73609MFV450
mFluor Violet 500 SE	NBP2-73609MFV500
mFluor Violet 610 SE	NBP2-73609MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73609

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DY682

Human Prolactin DuoSet ELISA, 15 Plate

(10 Publications)

1 Kit (for 15 Plates)

DY682

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-35120

Recombinant Mouse Prolactin Protein

10μg

NBP2-35120

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Sizes:

Sample Size Available

Sizes: Sample Size Available

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier-Free	NBP2-35120-1mg
Carrier-Free	NBP2-35120-100ug
Carrier-Free	NBP2-35120-500ug

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DPRL00

Human Prolactin Quantikine ELISA Kit

(5 Publications)

1 Kit

DPRL00

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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682-PL

Recombinant Human Prolactin Protein, CF

(7 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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DY1445

Mouse Prolactin DuoSet ELISA, 15 Plate

(4 Publications)

1 Kit (for 15 Plates)

DY1445

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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MAB11590

Prolactin Antibody (1085531) [Unconjugated]

100 μg

MAB11590

Western Blot shows lysates of human pituitary tissue. PVDF membrane was probed with 0.5 µg/ml of Mouse Anti-Human Prolactin Monoclonal Antibody (Catalog # MAB11590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Prolactin at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western shows lysates of human pituitary tissue, loaded at 0.2 mg/ml. A specific band was detected for Prolactin at approximately 29 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Prolactin Monoclonal Antibody (Catalog # MAB11590). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Prolactin was detected in immersion fixed paraffin-embedded sections of human pituitary using Mouse Anti-Human Prolactin Monoclonal Antibody (Catalog # mab11590) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11590U-100UG
Alexa Fluor 405	FAB11590V-100UG
Alexa Fluor 488	FAB11590G-100UG
Alexa Fluor 532	FAB11590X-100UG
Alexa Fluor 594	FAB11590T-100UG
Alexa Fluor 647	FAB11590R-100UG
Alexa Fluor 700	FAB11590N-100UG
Alexa Fluor 750	FAB11590S-100UG
Alexa Fluor Plus 405	FAB11590AFP405-100UG
Alexa Fluor Plus 488	FAB11590AFP488-100UG
Alexa Fluor Plus 555	FAB11590AFP555-100UG
Alexa Fluor Plus 594	FAB11590AFP594-100UG
Alexa Fluor Plus 647	FAB11590AFP647-100UG
Alexa Fluor Plus 680	FAB11590AFP680-100UG
Allophycocyanin	FAB11590A
Biotin	FAB11590B
CoraFluor 1	FAB11590CL1
DyLight 350	FAB11590D
DyLight 405	FAB11590E
DyLight 488	FAB11590K
DyLight 550	FAB11590L
DyLight 594	FAB11590M
DyLight 650	FAB11590W
DyLight 680	FAB11590Y
DyLight 755	FAB11590Z
FITC	FAB11590F
HRP	FAB11590H
Janelia Fluor 525	FAB11590JF525
Janelia Fluor 549	FAB11590I
Janelia Fluor 646	FAB11590J
Janelia Fluor 669	FAB11590JF669
PE	FAB11590P
PerCP	FAB11590C
mFluor Violet 450 SE	FAB11590MFV450
mFluor Violet 500 SE	FAB11590MFV500
mFluor Violet 610 SE	FAB11590MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11590-SP

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1445-PL

Recombinant Mouse Prolactin Protein

(4 Publications)

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	1445-PL-050/CF

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NBP3-49890

Monkey Prolactin ELISA Kit (Colorimetric)

1 Kit

NBP3-49890

Samples were spiked with high concentrations of Monkey PRL, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Samples were spiked with high concentrations of Monkey Prolactin, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Reactivity:

Pm

Species Glossary

Reactivity: Pm

Applications:

Applications:

Standard Curve:

0.63 - 40 ng/mL

Sensitivity:

0.38 ng/mL

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MAB1445

Prolactin Antibody (207518) [Unconjugated]

(1 Publication)

500 μg

MAB1445

Western blot shows lysates of mouse pituitary tissue and rat pituitary tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat Prolactin Monoclonal Antibody (Catalog # MAB1445) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1445U-100UG
Alexa Fluor 405	FAB1445V-100UG
Alexa Fluor 488	FAB1445G-100UG
Alexa Fluor 532	FAB1445X-100UG
Alexa Fluor 594	FAB1445T-100UG
Alexa Fluor 647	FAB1445R-100UG
Alexa Fluor 700	FAB1445N-100UG
Alexa Fluor 750	FAB1445S-100UG
Alexa Fluor Plus 405	FAB1445AFP405-100UG
Alexa Fluor Plus 488	FAB1445AFP488-100UG
Alexa Fluor Plus 555	FAB1445AFP555-100UG
Alexa Fluor Plus 594	FAB1445AFP594-100UG
Alexa Fluor Plus 647	FAB1445AFP647-100UG
Alexa Fluor Plus 680	FAB1445AFP680-100UG
Allophycocyanin	FAB1445A
Biotin	FAB1445B
CoraFluor 1	FAB1445CL1
DyLight 350	FAB1445D
DyLight 405	FAB1445E
DyLight 488	FAB1445K
DyLight 550	FAB1445L
DyLight 594	FAB1445M
DyLight 650	FAB1445W
DyLight 680	FAB1445Y
DyLight 755	FAB1445Z
FITC	FAB1445F
HRP	FAB1445H
Janelia Fluor 525	FAB1445JF525
Janelia Fluor 549	FAB1445I
Janelia Fluor 646	FAB1445J
Janelia Fluor 669	FAB1445JF669
PE	FAB1445P
PerCP	FAB1445C
mFluor Violet 450 SE	FAB1445MFV450
mFluor Violet 500 SE	FAB1445MFV500
mFluor Violet 610 SE	FAB1445MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1445-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

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