Prolactin R Antibody (U5)


Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561] - Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber more
Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561] - Mammary gland tissue from lactating light horse mare (equine) was incubated in anti-prolactin receptor monoclonal mouse antibody at 1:100 for 1 hour at 23C more
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561] - Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber more
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561] - Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber more
Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561] - Figure 1 shows immunohistochemical staining of PRL receptor in rat Islets of Langerhans using NB300-561.

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Eq, Ha, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP

Order Details

Prolactin R Antibody (U5) Summary

Purified rat liver PRL receptor.
This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml
Application Notes
May be useful in IHC, IP, and WB. WB: Detects an approx. 40 kDa and 100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. IHC: Staining of PRL receptor in rat Islets of Langerhans with NB 300-561 results in a staining pattern consistent with cytoplasmic vesicle staining. NB 300-561, after biotinylation, has been used to detect human lymphocyte PRL receptor by Flow Cytometry. NB 300-561 does not inhibit the interaction of prolactin with the receptor. Use in ICC/IF was reported in scientific literature (PMID: 29874677).
Read Publications using
NB300-561 in the following applications:

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 2707162). Bovine reactivity reported in scientific literature (PMID: 25212395). Mouse reactivity reported in scientific literature (PMID: 19211889). Hamster reactivity reported in scientific literature (PMID: 24142695). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Reconstitution Instructions
Reconstitute with 0.1 ml PBS.

Alternate Names for Prolactin R Antibody (U5)

  • delta 4-delta 7/11 truncated prolactin receptor
  • delta 4-SF1b truncated prolactin receptor
  • hPRL receptor
  • hPRLrI
  • PRLR
  • PRL-R
  • Prolactin R
  • prolactin receptor delta 7/11
  • prolactin receptor
  • ProlactinR
  • secreted prolactin binding protein


Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prolactin R Antibody (NB300-561)(13)

We have publications tested in 3 confirmed species: Human, Rat, Bovine.

We have publications tested in 2 applications: ICC/IF, WB.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB300-561 Applications Species
Wang B, Shi L, Men J et al. Controlled synchronization of prolactin/STAT5 and AKT1/mTOR in bovine mammary epithelial cells In Vitro Cell. Dev. Biol. Anim. 2020-02-20 [PMID: 32078727] (Human) Human
Wang B, Men J, Wang C et al. Laminin-dependent integrin beta 1 signaling regulates milk protein synthesis via prolactin/STAT5 pathway in bovine mammary epithelial cells Biochem. Biophys. Res. Commun. 2020-01-24 [PMID: 31987497] (WB, Bovine) WB Bovine
Ochoa-Amaya JE, Queiroz-Hazarbassanov N, Namazu LB et al. Short-Term Hyperprolactinemia Reduces the Expression of Purinergic P2X7 Receptors during Allergic Inflammatory Response of the Lungs Neuroimmunomodulation 2018 Jun 06 [PMID: 29874677] (ICC/IF, Rat) Neuroimmunomodulation. [PMID: 29874677] (ICC/IF, Rat) ICC/IF Rat
Perrot-Applanat M, Gualillo O, Buteau H, Edery M, Kelly PA. Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation. J. Cell Sci., 110: 1123-1132. 1997-01-01 [PMID: 9175708]
Gagnerault MC, Touraine P, Savino W, Kelly PA, Dardenne M. Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations. J Immunol. 1993 Jun 15;150(12):5673-81. 1993-01-01 [PMID: 8515082]
Dardenne M, de Moraes Mdo C, Kelly PA, Gagnerault MC. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endo., 134(5): 2108-2114. 1994-01-01 [PMID: 8156910]
Rui H, Lebrun JJ, Kirken RA, Kelly PA, Farrar WL. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endo., 135(4): 1299-1306. 1994-01-01 [PMID: 7925093]
Sorenson RL, Stout LE. Prolactin receptors and JAK2 in islets of Langerhans: an immunohistochemical analysis. Endo., 136(9): 4092-4098. 1995-01-01 [PMID: 7649117]
Royster M, Driscoll P, Kelly PA, Freemark M. The prolactin receptor in the fetal rat: cellular localization of messenger ribonucleic acid, immunoreactive protein, and ligand-binding activity and induction of expression in late gestation. Endo, 136(9): 3892-3900. 1995-01-01 [PMID: 7649097]
Katoh M, Raguet S, Zachwieja J, Djiane J, Kelly PA. Hepatic prolactin receptors in the rat: characterization using monoclonal antireceptor antibodies. Endo, 120: 739-749. 1987-01-01 [PMID: 3803302]
Show All 13 Publications.

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Product General Protocols

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WB Video Protocol
ICC/IF Video Protocol

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Gene Symbol PRLR