Prolactin R Antibody (U5)

Images

 
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561] - Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber ...read more
Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561] - Mammary gland tissue from lactating light horse mare (equine) was incubated in anti-prolactin receptor monoclonal mouse antibody at 1:100 for 1 hour at 23C ...read more
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561] - Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber ...read more
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (U5) [NB300-561] - Prolactin Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber ...read more
Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561] - Figure 1 shows immunohistochemical staining of PRL receptor in rat Islets of Langerhans using NB300-561.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, Ha, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC/IF
Clone
U5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Prolactin R Antibody (U5) Summary

Immunogen
Purified rat liver PRL receptor.
Epitope
This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.
Localization
Cytoplasmic
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRLR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry 5 ug/ml
  • Immunofluorescence 1:20
Application Notes
May be useful in IHC, IP, and WB. WB: Detects an approx. 40 kDa and 100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. IHC: Staining of PRL receptor in rat Islets of Langerhans with NB 300-561 results in a staining pattern consistent with cytoplasmic vesicle staining. NB 300-561, after biotinylation, has been used to detect human lymphocyte PRL receptor by Flow Cytometry. NB 300-561 does not inhibit the interaction of prolactin with the receptor.
Publications
Read Publications using NB300-561.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 2707162). Bovine reactivity reported in scientific literature (PMID: 25212395). Mouse reactivity reported in scientific literature (PMID: 19211889). Hamster reactivity reported in scientific literature (PMID: 24142695). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.1 ml PBS.

Alternate Names for Prolactin R Antibody (U5)

  • delta 4-delta 7/11 truncated prolactin receptor
  • delta 4-SF1b truncated prolactin receptor
  • hPRL receptor
  • hPRLrI
  • PRLR
  • PRL-R
  • Prolactin R
  • prolactin receptor delta 7/11
  • prolactin receptor
  • ProlactinR
  • secreted prolactin binding protein

Background

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prolactin R Antibody (NB300-561)(10)


Showing Publications 1 - 10 of 10.
Publications using NB300-561 Applications Species
Perrot-Applanat M, Gualillo O, Buteau H, Edery M, Kelly PA. Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation. J. Cell Sci., 110: 1123-1132. 1997 [PMID: 9175708]
Gagnerault MC, Touraine P, Savino W, Kelly PA, Dardenne M. Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations. J Immunol. 1993 Jun 15;150(12):5673-81. 1993 [PMID: 8515082]
Dardenne M, de Moraes Mdo C, Kelly PA, Gagnerault MC. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endo., 134(5): 2108-2114. 1994 [PMID: 8156910]
Rui H, Lebrun JJ, Kirken RA, Kelly PA, Farrar WL. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endo., 135(4): 1299-1306. 1994 [PMID: 7925093]
Sorenson RL, Stout LE. Prolactin receptors and JAK2 in islets of Langerhans: an immunohistochemical analysis. Endo., 136(9): 4092-4098. 1995 [PMID: 7649117]
Royster M, Driscoll P, Kelly PA, Freemark M. The prolactin receptor in the fetal rat: cellular localization of messenger ribonucleic acid, immunoreactive protein, and ligand-binding activity and induction of expression in late gestation. Endo, 136(9): 3892-3900. 1995 [PMID: 7649097]
Katoh M, Raguet S, Zachwieja J, Djiane J, Kelly PA. Hepatic prolactin receptors in the rat: characterization using monoclonal antireceptor antibodies. Endo, 120: 739-749. 1987 [PMID: 3803302]
Okamura H, Zachwieja J, Raguet S, Kelly PA. Characterization and applications of monoclonal antibodies to the prolactin receptor. Endo., 124(5): 2499-2508. 1989 [PMID: 2707162]
Dardenne M, Kelly PA, Bach JF, Savino W. Identification and functional activity of prolactin receptors in thymic epithelial cells. Proc. Natl. Acad. Sci., USA, 88: 9700-9704. 1991 [PMID: 1946390]
Rozakis-Adcock M, Kelly PA. Mutational analysis of the ligand-binding domain of the prolactin receptor. J. Biol. Chem., 266(25): 16472-16477. 1991 [PMID: 1885580]

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Prolactin R Antibody (NB300-561) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-561AF405
Alexa Fluor 488 Labeled NB300-561AF488
Alexa Fluor 647 Labeled NB300-561AF647
Alexa Fluor 700 Labeled NB300-561AF700
Allophycocyanin Labeled NB300-561APC
DyLight 350 Labeled NB300-561UV
DyLight 405 Labeled NB300-561V
DyLight 488 Labeled NB300-561G
DyLight 680 Labeled NB300-561FR
DyLight 755 Labeled NB300-561IR
PE Labeled NB300-561PE
PerCP Labeled NB300-561PCP

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Bioinformatics

Gene Symbol PRLR
Entrez
Uniprot