STAT5a/b Antibody [Unconjugated] - Pan Specific

Images

 
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen ...read more
BaF3 mouse pro-B cell line treated with 10 ng/mL Recombinant Mouse IL‑3 (Catalog # 403-ML) for 1 hour was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. STAT5a/b/DNA complexes ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5a/b at ...read more
Effects of hypoxia generated by using the insert-based enzymatic system on the phosphorylation of cell signalling molecules. Several molecules associated with cellular signalling and survival events are differentially ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

STAT5a/b Antibody [Unconjugated] - Pan Specific Summary

Immunogen
Human STAT5a and STAT5b synthetic peptide
aa 219-390
Specificity
Detects endogenous human and mouse STAT5A and STAT5B in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STAT5A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF2168 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT5a/b Antibody [Unconjugated] - Pan Specific

  • MGF
  • STAT5
  • STAT5A signal transducer and activator of transcription 5A
  • STAT5a/b

Background

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT5a/b Antibody (AF2168)(28)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, Flow Cytometry, Functional Assay, Neutralization, Western Blot.


Filter By Application
ChIP
(8)
Flow Cytometry
(1)
Functional Assay
(1)
Neutralization
(1)
Western Blot
(7)
All Applications
Filter By Species
Human
(5)
Mouse
(11)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using AF2168 Applications Species
Li H, Chumroenphat T, Boonarsa P et al. Evaluation of Roasting and Grilling Effects on Chemical Composition, Volatile Profiles, and Toxicity of Stink Bugs (Tessaratoma papillosa): Implications for Utilization as Functional Food Ingredients Foods (Basel, Switzerland) 2023-08-15 [PMID: 37628053]

Details:
Tessaratoma papillosa
Basu, R;Brody, R;Sandbhor, U;Kulkarni, P;Davis, E;Swegan, D;Caggiano, LJ;Brenya, E;Neggers, S;Kopchick, JJ; Structure and function of a dual antagonist of the human growth hormone and prolactin receptors with site-specific PEG conjugates The Journal of biological chemistry 2023-07-11 [PMID: 37442239] (Western Blot, Human) Western Blot Human
KA Read, DM Jones, S Pokhrel, EDS Hales, A Varkey, JA Tuazon, CD Eisele, O Abdouni, A Saadey, MR Leonard, RT Warren, MD Powell, JM Boss, EA Hemann, JS Yount, G Xin, HE Ghoneim, CJ Lio, AG Freud, PL Collins, KJ Oestreich Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity Nature Communications, 2023-03-24;14(1):1652. 2023-03-24 [PMID: 36964178] (ChIP, Mouse) ChIP Mouse
Duy Pham, Daniel J Silberger, Kim N Nguyen, Min Gao, Casey T Weaver, Robin D Hatton Batf stabilizes Th17 cell development via impaired Stat5 recruitment of Ets1‐Runx1 complexes The EMBO Journal 2023-04-17 [PMID: 36917143]
Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells Pharmaceuticals (Basel) 2022-09-08 [PMID: 36145344]
Al-Rawashde FA, Wan Taib WR, Ismail I et al. Thymoquinone Induces Downregulation of BCR-ABL/JAK/STAT Pathway and Apoptosis in K562 Leukemia Cells Asian Pacific journal of cancer prevention : APJCP 12/1/2021 [PMID: 34967577]
Angel Lin, Weam Othman Elbezanti, Alexis Schirling, Adel Ahmed, Rachel Van Duyne, Simon Cocklin et al. Alprazolam Prompts HIV-1 Transcriptional Reactivation and Enhances CTL Response Through RUNX1 Inhibition and STAT5 Activation Frontiers in Neurology 2021-07-22 [PMID: 34367046]
Xiaojing Yue, Daniela Samaniego‐Castruita, Edahí González‐Avalos, Xiang Li, Benjamin G Barwick, Anjana Rao Whole‐genome analysis of TET dioxygenase function in regulatory T cells EMBO reports 2021-08-04 [PMID: 34288360]
GM Wiedemann, EK Santosa, S Grassmann, S Sheppard, JB Le Luduec, NM Adams, C Dang, KC Hsu, JC Sun, CM Lau Deconvoluting global cytokine signaling networks in natural killer cells Nature Immunology, 2021-04-15;0(0):. 2021-04-15 [PMID: 33859404] (ChIP, Mouse) ChIP Mouse
AM Schoepf, S Salcher, P Obexer, R Gust Tackling resistance in chronic myeloid leukemia: Novel cell death modulators with improved efficacy European Journal of Medicinal Chemistry, 2021-02-18;216(0):113285. 2021-02-18 [PMID: 33662676] (Western Blot, Human) Western Blot Human
Show All 28 Publications.

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Bioinformatics

Gene Symbol STAT5A