STAT5a/b Antibody [Unconjugated] - Pan Specific

Images

 
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen ...read more
BaF3 mouse pro-B cell line treated with 10 ng/mL Recombinant Mouse IL‑3 (Catalog # 403-ML) for 1 hour was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. STAT5a/b/DNA complexes ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5a/b at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

STAT5a/b Antibody [Unconjugated] - Pan Specific Summary

Immunogen
Human STAT5a and STAT5b synthetic peptide
aa 219-390
Specificity
Detects endogenous human and mouse STAT5A and STAT5B in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STAT5A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 20 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF2168 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT5a/b Antibody [Unconjugated] - Pan Specific

  • MGF
  • STAT5
  • STAT5A signal transducer and activator of transcription 5A
  • STAT5a/b

Background

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT5a/b Antibody (AF2168)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ChIP, Western Blot.


Filter By Application
ChIP
(4)
Western Blot
(5)
All Applications
Filter By Species
Human
(2)
Mouse
(7)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF2168 Applications Species
SH Yip, N Romanò, P Gustafson, DJ Hodson, EJ Williams, IC Kokay, AO Martin, P Mollard, DR Grattan, SJ Bunn Elevated Prolactin during Pregnancy Drives a Phenotypic Switch in Mouse Hypothalamic Dopaminergic Neurons Cell Rep, 2019;26(7):1787-1799.e5. 2019 [PMID: 30759390] (Western Blot, Mouse) Western Blot Mouse
SS Ganaie, W Zou, P Xu, X Deng, S Kleiboeker, J Qiu Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex PLoS Pathog., 2017;13(5):e1006370. 2017 [PMID: 28459842] (Western Blot, Human) Western Blot Human
L Mokada-Gop, A Boeser, CH Lehmann, F Drepper, D Dudziak, B Warscheid, D Voehringer Identification of Novel STAT6-Regulated Proteins in Mouse B Cells by Comparative Transcriptome and Proteome Analysis J. Immunol, 2017;0(0):. 2017 [PMID: 28348271] (Western Blot, Mouse) Western Blot Mouse
Wagatsuma K, Tani-ichi S, Liang B, Shitara S, Ishihara K, Abe M, Miyachi H, Kitano S, Hara T, Nanno M, Ishikawa H, Sakimura K, Nakao M, Kimura H, Ikuta K STAT5 Orchestrates Local Epigenetic Changes for Chromatin Accessibility and Rearrangements by Direct Binding to the TCRgamma Locus. J Immunol, 2015;195(4):1804-14. 2015 [PMID: 26195811] (ChIP, Mouse) ChIP Mouse
Richard A, Tan C, Hawley E, Gomez-Rodriguez J, Goswami R, Yang X, Cruz A, Penumetcha P, Hayes E, Pelletier M, Gabay O, Walsh M, Ferdinand J, Keane-Myers A, Choi Y, O'Shea J, Al-Shamkhani A, Kaplan M, Gery I, Siegel R, Meylan F The TNF-family ligand TL1A and its receptor DR3 promote T cell-mediated allergic immunopathology by enhancing differentiation and pathogenicity of IL-9-producing T cells. J Immunol, 2015;194(8):3567-82. 2015 [PMID: 25786692] (ChIP, Mouse) ChIP Mouse
Zhang W, Gao C, Konopleva M, Chen Y, Jacamo R, Borthakur G, Cortes J, Ravandi F, Ramachandran A, Andreeff M Reversal of acquired drug resistance in FLT3-mutated acute myeloid leukemia cells via distinct drug combination strategies. Clin Cancer Res, 2014;20(9):2363-74. 2014 [PMID: 24619500] (Western Blot, Mouse) Western Blot Mouse
Liao W, Spolski R, Li P, Du N, West E, Ren M, Mitra S, Leonard W Opposing actions of IL-2 and IL-21 on Th9 differentiation correlate with their differential regulation of BCL6 expression. Proc Natl Acad Sci U S A, 2014;111(9):3508-13. 2014 [PMID: 24550509] (ChIP, Mouse) ChIP Mouse
Zhang, Qian, Wang, Hong Yi, Wei, Fang, Liu, Xiaobin, Paterson, Jennifer, Roy, Darshan, Mihova, Daniela, Woetmann, Anders, Ptasznik, Andrzej, Odum, Niels, Schuster, Stephen, Marafioti, Teresa, Riley, James L, Wasik, Mariusz Cutaneous T cell lymphoma expresses immunosuppressive CD80 (B7-1) cell surface protein in a STAT5-dependent manner. J Immunol, 2014;192(6):2913-9. 2014 [PMID: 24523507] (ChIP, Human) ChIP Human
Zhu J, Guo L, Min B, Watson CJ, Hu-Li J, Young HA, Tsichlis PN, Paul WE Growth factor independent-1 induced by IL-4 regulates Th2 cell proliferation. Immunity, 2002;16(5):733-44. 2002 [PMID: 12049724] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol STAT5A