STAT5a/b Antibody [Unconjugated] - Pan Specific


Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen more
BaF3 mouse pro-B cell line treated with 10 ng/mL Recombinant Mouse IL‑3 (Catalog # 403-ML) for 1 hour was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. STAT5a/b/DNA complexes more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5a/b at more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP

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STAT5a/b Antibody [Unconjugated] - Pan Specific Summary

Human STAT5a and STAT5b synthetic peptide
aa 219-390
Detects endogenous human and mouse STAT5A and STAT5B in Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using
AF2168 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT5a/b Antibody [Unconjugated] - Pan Specific

  • MGF
  • STAT5
  • STAT5A signal transducer and activator of transcription 5A
  • STAT5a/b


Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STAT5a/b Antibody (AF2168)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ChIP, Neutralization, Western Blot.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF2168 Applications Species
GM Wiedemann, EK Santosa, S Grassmann, S Sheppard, JB Le Luduec, NM Adams, C Dang, KC Hsu, JC Sun, CM Lau Deconvoluting global cytokine signaling networks in natural killer cells Nature Immunology, 2021;0(0):. 2021-01-01 [PMID: 33859404] (ChIP, Mouse) ChIP Mouse
AM Schoepf, S Salcher, P Obexer, R Gust Tackling resistance in chronic myeloid leukemia: Novel cell death modulators with improved efficacy European Journal of Medicinal Chemistry, 2021;216(0):113285. 2021-01-01 [PMID: 33662676] (Western Blot, Human) Western Blot Human
GM Wiedemann, S Grassmann, CM Lau, M Rapp, AV Villarino, C Friedrich, G Gasteiger, JJ O'Shea, JC Sun Divergent Role for STAT5 in the Adaptive Responses of Natural Killer Cells Cell Reports, 2020;33(11):108498. 2020-01-01 [PMID: 33326784] (ChIP, Mouse) ChIP Mouse
B Heizmann, S Le Gras, C Simand, P Marchal, S Chan, P Kastner Ikaros antagonizes DNA binding by STAT5 in pre-B cells PLoS ONE, 2020;15(11):e0242211. 2020-01-01 [PMID: 33180866] (ChIP, Mouse) ChIP Mouse
W Elbezanti, A Lin, A Schirling, A Jackson, M Marshall, RV Duyne, F Maldarelli, L Sardo, Z Klase Benzodiazepines Drive Alteration of Chromatin at the Integrated HIV-1 LTR Viruses, 2020;12(2):. 2020-01-01 [PMID: 32050449] (Neutralization, Human) Neutralization Human
SH Yip, N Romanò, P Gustafson, DJ Hodson, EJ Williams, IC Kokay, AO Martin, P Mollard, DR Grattan, SJ Bunn Elevated Prolactin during Pregnancy Drives a Phenotypic Switch in Mouse Hypothalamic Dopaminergic Neurons Cell Rep, 2019;26(7):1787-1799.e5. 2019-01-01 [PMID: 30759390] (Western Blot, Mouse) Western Blot Mouse
SS Ganaie, W Zou, P Xu, X Deng, S Kleiboeker, J Qiu Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex PLoS Pathog., 2017;13(5):e1006370. 2017-01-01 [PMID: 28459842] (Western Blot, Human) Western Blot Human
L Mokada-Gop, A Boeser, CH Lehmann, F Drepper, D Dudziak, B Warscheid, D Voehringer Identification of Novel STAT6-Regulated Proteins in Mouse B Cells by Comparative Transcriptome and Proteome Analysis J. Immunol, 2017;0(0):. 2017-01-01 [PMID: 28348271] (Western Blot, Mouse) Western Blot Mouse
Wagatsuma K, Tani-ichi S, Liang B, Shitara S, Ishihara K, Abe M, Miyachi H, Kitano S, Hara T, Nanno M, Ishikawa H, Sakimura K, Nakao M, Kimura H, Ikuta K STAT5 Orchestrates Local Epigenetic Changes for Chromatin Accessibility and Rearrangements by Direct Binding to the TCRgamma Locus. J Immunol, 2015;195(4):1804-14. 2015-01-01 [PMID: 26195811] (ChIP, Mouse) ChIP Mouse
Richard A, Tan C, Hawley E, Gomez-Rodriguez J, Goswami R, Yang X, Cruz A, Penumetcha P, Hayes E, Pelletier M, Gabay O, Walsh M, Ferdinand J, Keane-Myers A, Choi Y, O'Shea J, Al-Shamkhani A, Kaplan M, Gery I, Siegel R, Meylan F The TNF-family ligand TL1A and its receptor DR3 promote T cell-mediated allergic immunopathology by enhancing differentiation and pathogenicity of IL-9-producing T cells. J Immunol, 2015;194(8):3567-82. 2015-01-01 [PMID: 25786692] (ChIP, Mouse) ChIP Mouse
Show All 14 Publications.

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Gene Symbol STAT5A