Caspase-1 Antibody (14F468) - BSA Free

Simple Western: Caspase-1 Antibody (14F468) [NB100-56565] - Simple Western lane view shows a specific band for Caspase 1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry: Caspase-1 Antibody (14F468) [NB100-56565] - Activity of Casp1 in OHT-injured and normotensive control eyes. (A) Casp1 was detected by intraocular injection FLICA660-labeled substrate (green) in vivo 24 h after injury. Bright labeling (arrows) is evident in cells in the GCL and inner nuclear layer (INL) layers of the OHT-challenged retinas, a diffuse labeling of cell processes located in the IPL. Casp1 activity is diminished in Panx1-/- (Px1-/- OHT) retinas and WT retinas treated with probenecid (WT/Pbcd) at 12 h postinjury. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fnmol.2019.00036/full), licensed under a CC-BY licence.

Western Blot: Caspase-1 Antibody (14F468) [NB100-56565] - P. gingivalis and its OMVs differentially induce inflammasome signaling and pyroptosis in murine macrophages. BMM were infected as before (2 h at MOI of 25:1, see Materials and Methods) with viable P. gingivalis (Pg), heat-killed-Pg (HK-Pg), OMVs, or heat-inactivated-OMVs (HI-OMVs) and the activation of inflammasome components in the lysates [or supernatants (sup) where indicated] measured after 24 h by Western blot; beta-actin serves as a loading control throughout. Western blot data are representative of at least three independent experiments. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fcimb.2017.00351/full), licensed under a CC-BY licence.

Western Blot: Caspase-1 Antibody (14F468) [NB100-56565] - LukAB is a potent activator of Caspase 1. THP1 cells were intoxicated with 50 ng/mL LukAB for 1 hour and cell lysates were analyzed by immunoblot for Caspase 1 cleavage, which indicates activation. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004970) licensed under a CC-BY licence.

Immunohistochemistry: Caspase-1 Antibody (14F468) [NB100-56565] - Activity of Casp1 in OHT-injured and normotensive control eyes. (B) The analysis of the Casp1 immunolabeling (red, white arrows) in normotensive (NT control) and injured (OHT) retinas at 12 h and 24 h postinjury. Yellow arrows denote Casp1 colocalization with RGCs (RBPMS +, green) as well as with other cells at 24 h post-OHT. Bar, 25 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fnmol.2019.00036/full), licensed under a CC-BY licence.

Western Blot: Caspase-1 Antibody (14F468) [NB100-56565] - P. gingivalis and its OMVs differentially induce inflammasome signaling and pyroptosis in human macrophages. MDM were infected as before (2 h at MOI of 25:1, see Materials and Methods) with viable P. gingivalis (Pg), heat-killed-Pg (HK-Pg), OMVs, or heat-inactivated-OMVs (HI-OMVs) and the activation of inflammasome components in the lysates was measured after 24 h by Western blot; beta-actin serves as a loading control throughout. Western blot data are representative of at least three independent experiments. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fcimb.2017.00351/full), licensed under a CC-BY licence.

Western Blot: Caspase-1 Antibody (14F468) [NB100-56565] - Analysis of Caspase-1 using a Caspase-1 monoclonal antibody. Human HeLa (A) and mouse NIH3T3 lysate probed with Caspase-1 antibody at 0.5 ug/ml and 2 ug/ml, respectively.

Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Adenocarcinoma of the rectum stained with Caspase-1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Detection of Caspase-1 protein in a section of normal human colon using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Distinct cytoplasmic staining along with some nuclear positivity was observed in crypts/mucosa, and staining was found to be more intense in the absorptive columnar epithelial cells. [10X Magnification]

Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Normal lung from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). In this representative lung section, different type of cells including pseudostratified columnar epithelium of bronchiole and the simple squamous epithelium of alveoli may be seen to develop immunoreactivity for Caspase 1. [10X Magnification]

Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Normal skin from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Strong cytoplasmic/nuclear staining developed in all the epidermal cells, blood vessels and some cells of the dermal connective tissues layer. [10X Magnification]

Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Detection of Caspase-1 in a section of human ovarian cancer using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468).

Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Tissue section of human intestine using Caspase-1 antibody (clone 14F468) at 5ug/ml concentration (1:200 dilution). The primary antibody binding to Caspase 1 in cells was detected using HRP conjugated anti-Mouse secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This Caspase 1 antibody generated a diffused but specific cytoplasmic staining in columnar epithelia cells of villi, and a few cells depicted nuclear staining also. Only a subset of connective tissue cells in lamina propria depicted positivity (cytoplasmic) for this protein.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 14F468
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Caspase-1 Antibody (14F468) - BSA Free Summary

• Immunogen: Caspase-1 Antibody (14F468) was developed against two synthetic peptides from the human Caspase-1 protein (amino acids 371-390 and 31-45) [UniProt P29466].
• Localization: Cytoplasm, Nucleus
• Specificity: Caspase-1 Antibody (14F468) will recognize full-length Caspase-1 and cleaved Caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Simple Western 1:50
  • Western Blot 0.5-2 ug/ml
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30930743).
• Theoretical MW: 45.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 Whole Cell Lysate
  HeLa Whole Cell Lysate
  Jurkat Staurosporine Treated / Untreated Cell Lysate

Reactivity Notes

Immunogen's sequence similarity with other species: Porcine/Pig (85%), Equine/Horse (80%), Canine (70%). Rat reactivity reported in scientific literature (PMID: 22133203).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Caspase-1 Antibody (14F468) - BSA Free

• CASP1 nirs variant 1
• CASP1
• CASP-1
• caspase 1, apoptosis-related cysteine peptidase
• Caspase-1 subunit p10
• Caspase-1 subunit p20
• Caspase1
• Caspase-1
• EC 3.4.22.36
• EC:3.4.22.36
• ICE
• IL-1 beta-converting enzyme
• IL1BC
• IL-1BC
• IL1BCE
• IL1B-convertase
• interleukin 1, beta, convertase
• interleukin 1-B converting enzyme
• Interleukin-1 beta convertase
• Interleukin-1 beta-converting enzyme
• p45

Background

Caspase-1, also known as interleukin-1-beta (IL-1beta) -converting enzyme (ICE), is a member of the cysteine-aspartic protease (caspase) family (1). The caspase family are aspartic proteolytic enzymes that play a key role in apoptotic cell death and inflammation (1). Caspase-1 is synthesized as an inactive proenzyme with a theoretical molecular weight of approximately 45 kDa and is 404 amino acids (aa) in length. The caspase-1 protein structure contains an N-terminal CARD (caspase recruitment domain), a large 20kDa P20 subunit (aa 120-297), and a small 10kDa P10 subunit (aa 317-404) (1, 2). The proenzyme undergoes cleavage at aspartic acid (Asp) residues to produce the active caspase-1 enzyme which exists as a heterotetramer (homodimer of the P20 and P10 heterodimers) (2). Inflammasome complexes sense various microbial and endogenous signals, including ligand-receptor interaction and growth factor deprivation, which results in the processing of pro-caspase to active caspase-1 (3, 4).The active caspase-1 is responsible for the cleavage and maturation of pro-inflammatory cytokines IL-1beta and IL-18, and promotes an inflammatory form of cell death induced by bacterial pathogens called pyroptosis (3, 4). Mature IL-1beta has a role in the immune reaction and recruiting inflammatory cells to the site of infection while IL-18 plays a role in interferon-gamma production and promotes cytolytic activity in natural killer cells (4).

Given the role of IL-1beta in inflammation, it makes sense that many diseases and pathologies have been associated with dysregulation of caspase-1 activation and the inflammasome (3, 4). The inflammasome is a multiprotein complex comprised of Nod-like receptor (NLR) family members and the adapter ASC (apoptosis-associated speck-like protein containing a CARD) which are crucial for capase-1 activation (3-5). For instance, the neuronal apoptosis inhibitory protein (NAIP)/NLRC4 inflammasome has been associated with colorectal cancer, breast cancer, and glioma pathogenesis (5). Caspase-1 activation and mutations in the inflammasome have also been linked to Chron's disease and Alzheimer's disease (4). In addition to immune and inflammatory related disorder, the inflammasome has been linked to metabolic and obesity related disorders including diabetes and cardiovascular disease (6). Finally, caspase-1 deficient mice exhibit enhanced epithelial cell proliferation in the colon, increased tumor formation, and reduced apoptosis (1). A more thorough understanding of the inflammasome-caspase-1 signaling pathway will be important for understanding disease pathology and potential therapeutic development.

Alternative names for caspase-1 includes CASP1, CASP1 nirs variant 1, EC 3.4.22.36, ICE, IL-1 beta-converting enzyme, IL1BC, IL1BCE, IL1B-converstase, interleukin-1 beta convertase, and p45.

References

1. Shalini, S., Dorstyn, L., Dawar, S., & Kumar, S. (2015). Old, new and emerging functions of caspases. Cell death and differentiation. https://doi.org/10.1038/cdd.2014.216

2. Chang, H. Y., & Yang, X. (2000). Proteases for cell suicide: functions and regulation of caspases. Microbiology and molecular biology reviews: MMBR. https://doi.org/10.1128/mmbr.64.4.821-846.2000

3. Vanaja, S. K., Rathinam, V. A., & Fitzgerald, K. A. (2015). Mechanisms of inflammasome activation: recent advances and novel insights. Trends in cell biology. https://doi.org/10.1016/j.tcb.2014.12.009

4. Franchi, L., Eigenbrod, T., Munoz-Planillo, R., & Nunez, G. (2009). The inflammasome: a caspase-1-activation platform that regulates immune responses and disease pathogenesis. Nature immunology. https://doi.org/10.1038/ni.1703

5. Kay, C., Wang, R., Kirkby, M., & Man, S. M. (2020). Molecular mechanisms activating the NAIP-NLRC4 inflammasome: Implications in infectious disease, autoinflammation, and cancer. Immunological reviews. https://doi.org/10.1111/imr.12906

6. Pham, D., Park, P. (2020). Recent insights on modulation of inflammasomes by adipokines: a critical event for the pathogenesis of obesity and metabolism-associated diseases. Archives of Pharmacal Research. https://doi.org/10.1007/s12272-020-01274-7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-1 Antibody (NB100-56565)(63)

Publications using NB100-56565

• Sobrano Fais R, Menezes da Costa R, Carvalho Mendes A et al. NLRP3 activation contributes to endothelin-1-induced erectile dysfunction Journal of cellular and molecular medicine 2023-01-01 [PMID: 36515571] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:500
• Missiroli S, Perrone M, GafA R et al. PML at mitochondria-associated membranes governs a trimeric complex with NLRP3 and P2X7R that modulates the tumor immune microenvironment Cell death and differentiation 2022-11-30 [PMID: 36450825] (WB, Mouse)
• Luo T, Zhou X, Qin M et al. Corilagin Restrains NLRP3 Inflammasome Activation and Pyroptosis through the ROS/TXNIP/NLRP3 Pathway to Prevent Inflammation Oxidative medicine and cellular longevity 2022-10-17 [PMID: 36299604]
• Xie J, Zhu CL, Wan XJ et al. GSDMD-mediated NETosis promotes the development of acute respiratory distress syndrome European journal of immunology 2022-10-17 [PMID: 36250416] (WB, Mouse)
• Zhao K, Li S, Chen J, Jin Q Inhibitory Effect of Trihydroxy Isoflavone on Neuronal Apoptosis in Natural Aging Rats Disease Markers 2022-08-21 [PMID: 36046381] (WB, Rat)
• Zhang Z, Tan Q, Guo P et al. NLRP3 inflammasome-mediated choroid plexus hypersecretion contributes to hydrocephalus after intraventricular hemorrhage via phosphorylated NKCC1 channels Journal of neuroinflammation 2022-06-21 [PMID: 35729645] (WB, Rat)
• Wu X, Chen Y, Liu C et al. STING Induces Liver Ischemia-Reperfusion Injury by Promoting Calcium-Dependent Caspase 1-GSDMD Processing in Macrophages Oxidative Medicine and Cellular Longevity 2022-01-30 [PMID: 35281468] (IF/IHC, Mouse)
• Sousa AH, Do Vale GT, Nascimento JA Et al. Inhibition of iNOS protects against the deleterious effects of sub-lethal sepsis and ethanol in the cardiorenal system Canadian journal of physiology and pharmacology 2021-07-27 [PMID: 34314655]
• Cau SB, Bruder-Nascimento A, Silva MB et al. Angiotensin-II activates vascular inflammasome and induces vascular damage Vascular pharmacology 2021-06-09 [PMID: 34098096]
• El-Rous MA, Saber S, Raafat EM, Ahmed AAE Dapagliflozin, an SGLT2 inhibitor, ameliorates acetic acid-induced colitis in rats by targeting NF kappa B/AMPK/NLRP3 axis Inflammopharmacology 2021-05-17 [PMID: 34002329]
• Lin Y, Luo T, Weng A et al. Gallic Acid Alleviates Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Through Enhancing Nrf2 Signaling Frontiers in immunology 2020-12-07 [PMID: 33365024] (WB, Mouse)
• Bourgeois JR, Kalyanasundaram G, Figueroa C et al. A semi-automated brain atlas-based analysis pipeline for c-Fos immunohistochemical data J Neurosci Methods 2020-10-20 [PMID: 33091429] (WB, Rat)
• Lu Q, Zhang Y, Kasetti RB et al. Nervilifordin F alleviates intestinal ischemia/reperfusion-induced acute lung injury via inhibiting inflammasome and mTOR pathway Invest Ophthalmol Vis Sci 2020-10-08 [PMID: 33085740] (WB, Rat)
• Liu L, Chen M, Lin K et al. Inhibiting Caspase-12 Mediated Inflammasome Activation protects against Oxygen-Glucose Deprivation Injury in Primary Astrocytes Int. J. Med. Sci. 2020-07-24 [PMID: 32788872] (WB, ICC/IF, Mouse)
• Kerr N, De Rivero Vaccari Jp, Weaver C Et Al. Enoxaparin Attenuates Acute Lung Injury and Inflammasome Activation Following Traumatic Brain Injury J. Neurotrauma 2020-07-15 [PMID: 32669032] (WB, Human)
• Pan H, Lin Y, Dou J et al. Wedelolactone facilitates Ser/Thr phosphorylation of NLRP3 dependent on PKA signalling to block inflammasome activation and pyroptosis Cell Prolif. 2020-07-12 [PMID: 32656909] (WB, Mouse)
• Rimessi A, Pozzato C et al. Pharmacological modulation of mitochondrial calcium uniporter controls lung inflammation in cystic fibrosis. Sci Adv 2020-01-05 [PMID: 32494695] (WB, Human)
• Frew John W, Hawkes Jason E, Krueger James G A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa. F1000Research 2019-06-25 [PMID: 31281635] (IF/IHC, Human)
• Gao K, Zheng P, Yang T Et al. Tangshenping granule inhibits pyroptosis in a rat model of streptozotocin-induced diabetic nephropathy via the NLRP3/caspase-1/GSDMD pathway Journal of Traditional Chinese Medical Sciences 2021-10-01 (IHC-P, Rat)
• Landau D, Shukri N, Arazi E et al. Beneficiary Effects of Colchicine on Inflammation and Fibrosis in A Mouse Model of Chronic Kidney Disease Research Square 2021-12-07 (WB, Mouse)
• Xie WH, Ding J, Xie XX et al. Hepatitis B virus X protein promotes liver cell pyroptosis under oxidative stress through NLRP3 inflammasome activation Inflamm. Res. 2020-04-28 [PMID: 32347316] (WB, Human)
• Dang S, Zhang Zy, Li Kl et Al. Blockade of beta-adrenergic signaling suppresses inflammasome and alleviates cardiac fibrosis Ann Transl Med 2020-02-01 [PMID: 32175420] (WB, Human)
• Pereira C, Carlos D, Ferreira N et al. Mitochondrial DNA Promotes NLRP3 Inflammasome Activation and Contributes to Endothelial Dysfunction and Inflammation in Type 1 Diabetes Front. Physiol. 2020-01-17 [PMID: 32009974] (WB, Mouse)
• KOse-Vogel N, Stengel S, Gardey E, et al. Transcriptional Suppression of the NLRP3 Inflammasome and Cytokine Release in Primary Macrophages by Low-Dose Anthracyclines Cells 2019-12-28 [PMID: 31905600] (WB, Human)
• NS Ferreira, T Bruder-Nas, CA Pereira et al. NLRP3 Inflammasome and Mineralocorticoid Receptors Are Associated with Vascular Dysfunction in Type 2 Diabetes Mellitus Cells. [PMID: 31817997] (WB, Mouse)
• Chueh TH, Cheng YH, Chen KH, Chien CT Thromboxane A2 Synthase and Thromboxane Receptor Deletion Reduces Ischaemia/Reperfusion-Evoked Inflammation, Apoptosis, Autophagy and Pyroptosis Thromb. Haemost. 2019-11-30 [PMID: 31785598] (WB)
• Madsen PM, Desu HL, Pablo de Rivero Vaccari J et al. Oligodendrocytes modulate the immune-inflammatory response in EAE via TNFR2 signaling Brain Behav. Immun. 2019-11-27 [PMID: 31785393] (WB, Mouse)
• Wang J, Li R, Peng Z et al. HMGB1 participates in LPS induced acute lung injury by activating the AIM2 inflammasome in macrophages and inducing polarization of M1 macrophages via TLR2, TLR4, and RAGE/NF-kappa B signaling pathways Int. J. Mol. Med. 2019-11-12 [PMID: 31746367] (WB, Mouse)
• Fais RS, Rodrigues FL, Pereira CA et al. The inflammasome NLRP3 plays a dual role on mouse corpora cavernosa relaxation Sci Rep 2019-11-07 [PMID: 31700106] (WB, Mouse)
• Li Y, Su Y, Zhou T et al. Activation of the NLRP3 Inflammasome Pathway by Prokineticin 2 in Testicular Macrophages of Uropathogenic Escherichia coli- Induced Orchitis Front Immunol 2019-08-14 [PMID: 31474981] (WB, Rat)
• Pronin A, Pham D, An W et al. Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury Front Mol Neurosci 2019-03-13 [PMID: 30930743] (IHC-Fr, Mouse)
• Mejias NH, Martinez CC, Stephens ME, de Rivero Vaccari JP. Contribution of the inflammasome to inflammaging. J Inflamm (Lond). 2018-11-16 [PMID: 30473634] (WB, Mouse)
• Kerr N, Garcia-Contreras M, Abbassi S et al. Inflammasome Proteins in Serum and Serum-Derived Extracellular Vesicles as Biomarkers of Stroke. Front Mol Neurosci 2018-09-04 [PMID: 30233311] (WB, Human)
• Raval A, Schatz M, Bhattacharya P et al. Whole Body Vibration Therapy after Ischemia Reduces Brain Damage in Reproductively Senescent Female Rats. (www.preprints.org 2018-07-16 [PMID: 30217051] (WB, Rat)
• Furuya MY, Asano T, Sumichika Y et al. Tofacitinib inhibits granulocyte-macrophage colony-stimulating factor-induced NLRP3 inflammasome activation in human neutrophils. Arthritis Res. Ther. 2018-08-29 [PMID: 30157949] (WB, Human)
• d'Adesky ND, de Rivero Vaccari JP, Bhattacharya P et al. Nicotine Alters Estrogen Receptor-Beta-Regulated Inflammasome Activity and Exacerbates Ischemic Brain Damage in Female Rats Int J Mol Sci 2018-04-30 [PMID: 29710856] (WB, Rat)
• Chen DY, Chen YM, Chen HH et al. Human parvovirus B19 nonstructural protein NS1 activates NLRP3 inflammasome signaling in adultonset Still's disease. Mol Med Rep 2018-02-01 [PMID: 29257322]
• Fleetwood AJ, Lee MKS, Singleton W et al. Metabolic Remodeling, Inflammasome Activation, and Pyroptosis in Macrophages Stimulated by Porphyromonas gingivalis and Its Outer Membrane Vesicles Front Cell Infect Microbiol 2017-08-21 [PMID: 28824884] (WB, Human)
• Hua X, Yuan X et al. Desiccating stress worsens alkali burn injury by magnifying caspase-8-induced imbalance of NLRP3 and NLRP6. J Allergy Clin Immunol 2017-01-10 [PMID: 28479336] (IF/IHC, Mouse)
• Levesque SA, Pare A, Mailhot B et al. Myeloid cell transmigration across the CNS vasculature triggers IL-1beta-driven neuroinflammation during autoimmune encephalomyelitis in mice. J. Exp. Med. 2016-05-30 [PMID: 27139491] (WB, Mouse)
• Lin QR, Li CG, Zha QB et al. Gossypol induces pyroptosis in mouse macrophages via a non-canonical inflammasome pathway. Toxicol. Appl. Pharmacol. 2016-02-01 [PMID: 26765310] (WB, Rat)
• Fernandez MV, Miller E, Krammer F et al. Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells. J. Leukoc. Biol. 2016-05-01 [PMID: 26574023] (WB, Human)
• Melehani JH, James DB, DuMont AL et al. Staphylococcus aureus Leukocidin A/B (LukAB) Kills Human Monocytes via Host NLRP3 and ASC when Extracellular, but Not Intracellular PLoS Pathog. 2015-06-01 [PMID: 26069969] (WB, Human)
• Queen J, Agarwal S, Dolores JS et al. Mechanisms of inflammasome activation by Vibrio cholerae secreted toxins varies with strain biotype Infect. Immun. 2015-04-06 [PMID: 25847959] (WB, Human)
  
  Details:
  Caspase 1 antibody was used for WB analysis of lysates from THP 1 cells/ human leukemic monocyte cell line treated or not with MOI 25 for 5 min with LPS, wild-type Vibrio cholerae El Tor strain P27459 or an isogenic hlyA deletion strain or with classical strain O395 or delta-ctxA or delta- ctxAB mutants (Suppl. Fig. 7)
• Rimessi A, Bezzerri V, Patergnani S et al. Mitochondrial Ca(2+)-dependent NLRP3 activation exacerbates the Pseudomonas aeruginosa-driven inflammatory response in cystic fibrosis Nat Commun. 2015-02-04 [PMID: 25648527] (WB, Human)
• de Rivero Vaccari JP, Brand F, Adamczak S et al. Exosome-mediated inflammasome signaling after central nervous system injury J. Neurochem. 2015-01-27 [PMID: 25628216] (IHC-P, WB, Human)
• Paciello I, Silipo A, Lembo-Fazio L et al. Intracellular Shigella remodels its LPS to dampen the innate immune recognition and evade inflammasome activation. Proc Natl Acad Sci U S A 2013-11-12 [PMID: 24167293] (WB, Mouse)
• Zhang X, Ibrahim E, de Rivero Vaccari JP et al. Involvement of the inflammasome in abnormal semen quality of men with spinal cord injury. Fertil Steril. 2013-01-01 [PMID: 23040525]
• Taxman DJ, Swanson KV, Broglie PM et al. Porphyromonas gingivalis mediates inflammasome repression in polymicrobial cultures through a novel mechanism involving reduced endocytosis. J Biol Chem. 2012-09-21 [PMID: 22843689]
• Tomura S, de Rivero Vaccari JP, Keane RW et al. Effects of therapeutic hypothermia on inflammasome signaling after traumatic brain injury. J Cereb Blood Flow Metab. 2012-10-01 [PMID: 22781337]
• de Rivero Vaccari JP, Sawaya ME, Brand F 3rd et al. Caspase-1 level is higher in the scalp in androgenetic alopecia. Dermatol Surg. 2012-07-01 [PMID: 22404322]
• de Rivero Vaccari JP, Bastien D, Yurcisin G et al. P2X4 receptors influence inflammasome activation after spinal cord injury. J Neurosci. 2012-02-29 [PMID: 22378878]
• Mawhinney Lana J, de Rivero Vaccari Juan Pablo, Dale Gordon A et al. Heightened inflammasome activation is linked to age-related cognitive impairment in Fischer 344 rats. BMC Neurosci. 2011-01-01 [PMID: 22133203] (WB, Rat)
• Nadeau Sylvain, Filali Mohammed, Zhang Ji et al. Functional recovery after peripheral nerve injury is dependent on the pro-inflammatory cytokines IL-1beta and TNF: implications for neuropathic pain. J Neurosci. 2011-08-31 [PMID: 21880915] (Mouse)
• Taxman DJ, Holley-Guthrie EA, Huang MT et al. The NLR adaptor ASC/PYCARD regulates DUSP10, mitogen-activated protein kinase (MAPK), and chemokine induction independent of the inflammasome. J Biol Chem. 2011-06-03 [PMID: 21487011]
• Atay S, Gercel-Taylor C, Taylor DD. Human trophoblast-derived exosomal fibronectin induces pro-inflammatory IL-1B production by macrophages. Am J Reprod Immunol. 2011-10-01 [PMID: 21410811]
• Rawat R, Cohen TV, Ampong B et al. Inflammasome up-regulation and activation in dysferlin-deficient skeletal muscle. Am J Pathol. 2010-06-01 [PMID: 20413686]
• Banerjee M, Datta M, Majumder P et al. Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1. Nucleic Acids Res. 2010-01-01 [PMID: 19934260]
• Craven RR, Gao X, Allen IC et al. Staphylococcus aureus alpha-hemolysin activates the NLRP3-inflammasome in human and mouse monocytic cells. PLoS One. 2009-10-14 [PMID: 19826485]
• Duncan JA, Gao X, Huang MT et al. Neisseria gonorrhoeae activates the proteinase cathepsin B to mediate the signaling activities of the NLRP3 and ASC-containing inflammasome. J Immunol. 2009-05-15 [PMID: 19414800]
• Hurst J, Prinz N, Lorenz M et al. TLR7 and TLR8 ligands and antiphospholipid antibodies show synergistic effects on the induction of IL-1beta and caspase-1 in monocytes and dendritic cells. Immunobiology. 2009-01-01 [PMID: 19249118]
• Dostert C, Petrilli V, Van Bruggen R et al. Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica. Science. 2008-05-02 [PMID: 18403674]
• Dorfleutner A, Bryan NB, Talbott SJ et al. Cellular pyrin domain-only protein 2 is a candidate regulator of inflammasome activation. Infect Immun. 2007-03-01 [PMID: 17178784]
  
  Details:
  Western blot: Fig 3D (transfected cells), Fig 3E (Jurkat cells)

FAQs for Caspase-1 Antibody (NB100-56565). (Showing 1 - 2 of 2 FAQs).

1. We have purchased "NB100-56565 Caspase-1 Antibody (14F468)" for our experiments. It is mentioned in product data sheet that it can detect the cleave forms of Caspase-1 as well. Can you kindly tell me the expected size of cleaved subforms on WB, so that I could be sure about my results?
   • The antibody will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein. In short this antibody can recognize any protein species, after processed by protease in vivo, that contains the region of a.a. 371 - 390.
2. We are interested in your items, NB100-56565 and NB100-56564 and I would like to use them for recognizing the cleaved form of Caspase-1. Would you please let me know if NB100-56565 and NB100-56564 recognize the Caspase-1 p10 or p20? If so, could you please provide me any supporting data of them?
   • For NB100-56565 & NB100-56564 it will recognize the full length form and the p10 as the immunogen. A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen. The p10 subunit is aa 307-404. If you need an antibody to the p20 subunit:http://www.novusbio.com/Caspase-1-Antibody_NBP1-45433.html. This will bind to the the region around aa 132. The p20 subunit is aa 120-297.

Bioinformatics

• Gene Symbol: CASP1
• Entrez:
  • 834(Human)
  • 12362(Mouse)
  • 25166(Rat)
• Uniprot:
  • P29466(Human)