Caspase-1 Antibody (14F468)

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Western Blot: Caspase-1 Antibody (14F468) [NB100-56565] - Analysis of Caspase-1 using a Caspase-1 monoclonal antibody. Human HeLa (A) and mouse NIH3T3 lysate probed with Caspase-1 antibody at 0.5 ug/ml and 2 ug/ml, ...read more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Tissue section of human intestine using Caspase-1 antibody (clone 14F468) at 5ug/ml concentration (1:200 dilution). The primary antibody binding ...read more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Adenocarcinoma of the rectum stained with Caspase-1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed ...read more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Detection of Caspase-1 protein in a section of normal human colon using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Distinct ...read more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Normal lung from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). In this representative lung section, different type of ...read more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Normal skin from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Strong cytoplasmic/nuclear staining developed in all ...read more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Detection of Caspase-1 in a section of human ovarian cancer using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468).
Simple Western: Caspase-1 Antibody (14F468) [NB100-56565] - Simple Western lane view shows a specific band for Caspase 1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clone
14F468
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Caspase-1 Antibody (14F468) Summary

Immunogen
Two synthetic peptides from the human Caspase-1 protein (amino acids 371-390 and 31-45) [UniProt P29466].
Localization
Cytoplasm, Nucleus
Specificity
The antibody will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Simple Western 1:50
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Lysate (NB800-PC8)
HeLa Lysate (NB800-PC1)
Caspase-1 Lysate (NBL1-08705)
Caspase-1 Lysate (NBP2-66281)
Publications
Read Publications using
NB100-56565 in the following applications:

  • 1 publication
  • WB
    11 publications

Reactivity Notes

Immunogen's sequence similarity with other species: Porcine/Pig (85%), Equine/Horse (80%), Canine (70%). Rat reactivity reported in scientific literature (PMID: 22133203).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Caspase-1 Antibody (14F468)

  • CASP1
  • CASP-1
  • caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta
  • caspase 1, apoptosis-related cysteine protease (interleukin 1, beta
  • Caspase1
  • Caspase-1
  • EC 3.4.22
  • EC 3.4.22.36
  • ICE
  • ICEP45
  • IL-1 beta-converting enzyme
  • IL1BC CASP1 nirs variant 1
  • IL-1BC
  • IL1BCE
  • IL1B-convertase
  • interleukin 1-B converting enzyme
  • interleukin 1-beta convertase
  • Interleukin-1 beta convertase
  • Interleukin-1 beta-converting enzyme
  • p45

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for Caspase-1 Antibody (NB100-56565)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(11)
All Applications
Filter By Species
Human
(6)
Mouse
(3)
Rat
(3)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB100-56565 Applications Species
d'Adesky ND, de Rivero Vaccari JP, Bhattacharya P et al. Nicotine Alters Estrogen Receptor-Beta-Regulated Inflammasome Activity and Exacerbates Ischemic Brain Damage in Female Rats Int J Mol Sci Apr 30 2018 [PMID: 29710856] (WB, Rat) WB Rat
Fleetwood AJ, Lee MKS, Singleton W et al. Metabolic Remodeling, Inflammasome Activation, and Pyroptosis in Macrophages Stimulated by Porphyromonas gingivalis and Its Outer Membrane Vesicles Front Cell Infect Microbiol 2017 Aug 21 [PMID: 28824884] (WB, Human) WB Human
Levesque SA, Pare A, Mailhot B et al. Myeloid cell transmigration across the CNS vasculature triggers IL-1beta-driven neuroinflammation during autoimmune encephalomyelitis in mice. J. Exp. Med. May 30 2016 [PMID: 27139491] (WB, Mouse) WB Mouse
Lin QR, Li CG, Zha QB et al. Gossypol induces pyroptosis in mouse macrophages via a non-canonical inflammasome pathway. Toxicol. Appl. Pharmacol. 2016 Feb 01 [PMID: 26765310] (WB, Rat) WB Rat
Fernandez MV, Miller E, Krammer F et al. Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells. J. Leukoc. Biol. May 1 2016 [PMID: 26574023] (WB, Human) WB Human
Melehani JH, James DB, DuMont AL et al. Staphylococcus aureus Leukocidin A/B (LukAB) Kills Human Monocytes via Host NLRP3 and ASC when Extracellular, but Not Intracellular PLoS Pathog. 2015 Jun 01 [PMID: 26069969] (WB, Human) WB Human
Queen J, Agarwal S, Dolores JS et al. Mechanisms of inflammasome activation by Vibrio cholerae secreted toxins varies with strain biotype Infect. Immun. 2015 Apr 06 [PMID: 25847959] (WB, Human)

Details:
Caspase 1 antibody was used for WB analysis of lysates from THP 1 cells/ human leukemic monocyte cell line treated or not with MOI 25 for 5 min with LPS, wild-type Vibrio cholerae El Tor strain P27459 or an isogenic hlyA deletion strain or with classical strain O395 or delta-ctxA or delta- ctxAB mutants (Suppl. Fig. 7)
WB Human
Rimessi A, Bezzerri V, Patergnani S et al. Mitochondrial Ca(2+)-dependent NLRP3 activation exacerbates the Pseudomonas aeruginosa-driven inflammatory response in cystic fibrosis Nat Commun. 2015 Feb 04 [PMID: 25648527] (WB, Human) WB Human
de Rivero Vaccari JP, Brand F, Adamczak S et al. Exosome-mediated inflammasome signaling after central nervous system injury J. Neurochem. 2015 Jan 27 [PMID: 25628216] (WB, IHC-P, Human) WB, IHC-P Human
Paciello I, Silipo A, Lembo-Fazio L et al. Intracellular Shigella remodels its LPS to dampen the innate immune recognition and evade inflammasome activation. Proc Natl Acad Sci U S A 2013 Nov 12 [PMID: 24167293] (WB, Mouse) WB Mouse
Show All 26 Publications.

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Product General Protocols

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FAQs for Caspase-1 Antibody (NB100-56565). (Showing 1 - 2 of 2 FAQs).

  1. We have purchased "NB100-56565 Caspase-1 Antibody (14F468)" for our experiments. It is mentioned in product data sheet that it can detect the cleave forms of Caspase-1 as well. Can you kindly tell me the expected size of cleaved subforms on WB, so that I could be sure about my results.
    • The antibody will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein. In short this antibody can recognize any protein species, after processed by protease in vivo, that contains the region of a.a. 371 - 390.
  2. We are interested in your items, NB100-56565 and NB100-56564 and I would like to use them for recognizing the cleaved form of caspase 1. Would you please let me know if NB100-56565 and NB100-56564 recognize the caspase-1 p10 or p20 ? If so, could you please provide me any supporting data of them?
    • For NB100-56565 & NB100-56564 it will recognize the full length form and the p10 as the immunogen. A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen. The p10 subunit is aa 307-404. If you need an antibody to the p20 subunit:http://www.novusbio.com/Caspase-1-Antibody_NBP1-45433.html. This will bind to the the region around aa 132. The p20 subunit is aa 120-297.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56565AF405
Alexa Fluor 488 Labeled NB100-56565AF488
Alexa Fluor 647 Labeled NB100-56565AF647
Alexa Fluor 700 Labeled NB100-56565AF700
Biotin Labeled NB100-56565B
DyLight 350 Labeled NB100-56565UV
DyLight 405 Labeled NB100-56565V
DyLight 488 Labeled NB100-56565G
DyLight 550 Labeled NB100-56565R
DyLight 650 Labeled NB100-56565C
DyLight 680 Labeled NB100-56565FR
DyLight 755 Labeled NB100-56565IR
HRP Labeled NB100-56565H

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Blogs on Caspase-1.

The inflammasome: an inflammation-initiating machine, Novus Biologicals
By Stephanie Melchor The inflammasome is a large, multimeric protein complex found primarily in innate immune cells, which are white blood cells that can attack a wide range of pathogenic threats. Three main elements...  Read full blog post.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

Caspase 10 - an initiator caspase in the extrinsic death receptor pathway
Apoptosis, also called programmed cell death, is an essential process in development and disease. The signaling networks that carry out apoptosis is consists of a series of endoproteases called caspases which are synthesized as inactive zymogens....  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 1 - activating innate immune responses following infection or injury
Caspase-1 is an enzyme involved in the conversion of interleukin-1 into its active secreted form. Interleukin-1 mediates inflammatory responses during infection and disease. Caspase-1 is recruited to and activated by the inflammasome complex (1). Un...  Read full blog post.

Caspase-4 - a human protease with roles in inflammation and immunity
Caspases are a family of cysteine-aspartic acid proteases that cleave caspase proenzymes as well as other protein substrates. Caspases are well known for their role in apoptosis, but they also play a significant role in other cellular processes inc...  Read full blog post.

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Bioinformatics

Gene Symbol CASP1
Entrez
Uniprot