Caspase-1 Antibody (14F468) - BSA Free


Simple Western: Caspase-1 Antibody (14F468) [NB100-56565] - Simple Western lane view shows a specific band for Caspase 1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the more
Biological Strategies: Immunohistochemistry: Caspase-1 Antibody (14F468) [NB100-56565] - Activity of Casp1 in OHT-injured and normotensive control eyes. (A) Casp1 was detected by intraocular injection more
Biological Strategies: Western Blot: Caspase-1 Antibody (14F468) [NB100-56565] - P. gingivalis and its OMVs differentially induce inflammasome signaling and pyroptosis in murine macrophages. BMM were infected as more
Western Blot: Caspase-1 Antibody (14F468) [NB100-56565] - LukAB is a potent activator of Caspase 1. THP1 cells were intoxicated with 50 ng/mL LukAB for 1 hour and cell lysates were analyzed by immunoblot for Caspase 1 more
Biological Strategies: Immunohistochemistry: Caspase-1 Antibody (14F468) [NB100-56565] - Activity of Casp1 in OHT-injured and normotensive control eyes. (B) The analysis of the Casp1 immunolabeling (red, white more
Western Blot: Caspase-1 Antibody (14F468) [NB100-56565] - P. gingivalis and its OMVs differentially induce inflammasome signaling and pyroptosis in human macrophages. MDM were infected as before (2 h at MOI of 25:1, see more
Western Blot: Caspase-1 Antibody (14F468) [NB100-56565] - Analysis of Caspase-1 using a Caspase-1 monoclonal antibody. Human HeLa (A) and mouse NIH3T3 lysate probed with Caspase-1 antibody at 0.5 ug/ml and 2 ug/ml, more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Adenocarcinoma of the rectum stained with Caspase-1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Detection of Caspase-1 protein in a section of normal human colon using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Distinct more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Normal lung from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). In this representative lung section, different type of more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Normal skin from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Strong cytoplasmic/nuclear staining developed in all more
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Detection of Caspase-1 in a section of human ovarian cancer using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468).
Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [NB100-56565] - Tissue section of human intestine using Caspase-1 antibody (clone 14F468) at 5ug/ml concentration (1:200 dilution). The primary antibody binding more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
BSA Free
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

Caspase-1 Antibody (14F468) - BSA Free Summary

Caspase-1 Antibody (14F468) was developed against two synthetic peptides from the human Caspase-1 protein (amino acids 371-390 and 31-45) [UniProt P29466].
Cytoplasm, Nucleus
Caspase-1 Antibody (14F468) will recognize full-length Caspase-1 and cleaved Caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Simple Western 1:50
  • Western Blot 0.5-2 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30930743).
Theoretical MW
45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
NIH 3T3 Whole Cell Lysate
HeLa Whole Cell Lysate
Jurkat Staurosporine Treated / Untreated Cell Lysate
Read Publications using
NB100-56565 in the following applications:

Reactivity Notes

Immunogen's sequence similarity with other species: Porcine/Pig (85%), Equine/Horse (80%), Canine (70%). Rat reactivity reported in scientific literature (PMID: 22133203).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for Caspase-1 Antibody (14F468) - BSA Free

  • CASP1 nirs variant 1
  • CASP1
  • CASP-1
  • caspase 1, apoptosis-related cysteine peptidase
  • Caspase-1 subunit p10
  • Caspase-1 subunit p20
  • Caspase1
  • Caspase-1
  • EC
  • EC:
  • ICE
  • IL-1 beta-converting enzyme
  • IL1BC
  • IL-1BC
  • IL1BCE
  • IL1B-convertase
  • interleukin 1, beta, convertase
  • interleukin 1-B converting enzyme
  • Interleukin-1 beta convertase
  • Interleukin-1 beta-converting enzyme
  • p45


Caspase-1, also known as interleukin-1-beta (IL-1beta) -converting enzyme (ICE), is a member of the cysteine-aspartic protease (caspase) family (1). The caspase family are aspartic proteolytic enzymes that play a key role in apoptotic cell death and inflammation (1). Caspase-1 is synthesized as an inactive proenzyme with a theoretical molecular weight of approximately 45 kDa and is 404 amino acids (aa) in length. The caspase-1 protein structure contains an N-terminal CARD (caspase recruitment domain), a large 20kDa P20 subunit (aa 120-297), and a small 10kDa P10 subunit (aa 317-404) (1, 2). The proenzyme undergoes cleavage at aspartic acid (Asp) residues to produce the active caspase-1 enzyme which exists as a heterotetramer (homodimer of the P20 and P10 heterodimers) (2). Inflammasome complexes sense various microbial and endogenous signals, including ligand-receptor interaction and growth factor deprivation, which results in the processing of pro-caspase to active caspase-1 (3, 4).The active caspase-1 is responsible for the cleavage and maturation of pro-inflammatory cytokines IL-1beta and IL-18, and promotes an inflammatory form of cell death induced by bacterial pathogens called pyroptosis (3, 4). Mature IL-1beta has a role in the immune reaction and recruiting inflammatory cells to the site of infection while IL-18 plays a role in interferon-gamma production and promotes cytolytic activity in natural killer cells (4).

Given the role of IL-1beta in inflammation, it makes sense that many diseases and pathologies have been associated with dysregulation of caspase-1 activation and the inflammasome (3, 4). The inflammasome is a multiprotein complex comprised of Nod-like receptor (NLR) family members and the adapter ASC (apoptosis-associated speck-like protein containing a CARD) which are crucial for capase-1 activation (3-5). For instance, the neuronal apoptosis inhibitory protein (NAIP)/NLRC4 inflammasome has been associated with colorectal cancer, breast cancer, and glioma pathogenesis (5). Caspase-1 activation and mutations in the inflammasome have also been linked to Chron's disease and Alzheimer's disease (4). In addition to immune and inflammatory related disorder, the inflammasome has been linked to metabolic and obesity related disorders including diabetes and cardiovascular disease (6). Finally, caspase-1 deficient mice exhibit enhanced epithelial cell proliferation in the colon, increased tumor formation, and reduced apoptosis (1). A more thorough understanding of the inflammasome-caspase-1 signaling pathway will be important for understanding disease pathology and potential therapeutic development.

Alternative names for caspase-1 includes CASP1, CASP1 nirs variant 1, EC, ICE, IL-1 beta-converting enzyme, IL1BC, IL1BCE, IL1B-converstase, interleukin-1 beta convertase, and p45.


1. Shalini, S., Dorstyn, L., Dawar, S., & Kumar, S. (2015). Old, new and emerging functions of caspases. Cell death and differentiation.

2. Chang, H. Y., & Yang, X. (2000). Proteases for cell suicide: functions and regulation of caspases. Microbiology and molecular biology reviews: MMBR.

3. Vanaja, S. K., Rathinam, V. A., & Fitzgerald, K. A. (2015). Mechanisms of inflammasome activation: recent advances and novel insights. Trends in cell biology.

4. Franchi, L., Eigenbrod, T., Munoz-Planillo, R., & Nunez, G. (2009). The inflammasome: a caspase-1-activation platform that regulates immune responses and disease pathogenesis. Nature immunology.

5. Kay, C., Wang, R., Kirkby, M., & Man, S. M. (2020). Molecular mechanisms activating the NAIP-NLRC4 inflammasome: Implications in infectious disease, autoinflammation, and cancer. Immunological reviews.

6. Pham, D., Park, P. (2020). Recent insights on modulation of inflammasomes by adipokines: a critical event for the pathogenesis of obesity and metabolism-associated diseases. Archives of Pharmacal Research.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-1 Antibody (NB100-56565)(63)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using NB100-56565 Applications Species
Sobrano Fais R, Menezes da Costa R, Carvalho Mendes A et al. NLRP3 activation contributes to endothelin-1-induced erectile dysfunction Journal of cellular and molecular medicine 2023-01-01 [PMID: 36515571] (WB, Mouse)

Dilution used in WB 1:500
WB Mouse
Missiroli S, Perrone M, GafA R et al. PML at mitochondria-associated membranes governs a trimeric complex with NLRP3 and P2X7R that modulates the tumor immune microenvironment Cell death and differentiation 2022-11-30 [PMID: 36450825] (WB, Mouse) WB Mouse
Luo T, Zhou X, Qin M et al. Corilagin Restrains NLRP3 Inflammasome Activation and Pyroptosis through the ROS/TXNIP/NLRP3 Pathway to Prevent Inflammation Oxidative medicine and cellular longevity 2022-10-17 [PMID: 36299604]
Xie J, Zhu CL, Wan XJ et al. GSDMD-mediated NETosis promotes the development of acute respiratory distress syndrome European journal of immunology 2022-10-17 [PMID: 36250416] (WB, Mouse) WB Mouse
Zhao K, Li S, Chen J, Jin Q Inhibitory Effect of Trihydroxy Isoflavone on Neuronal Apoptosis in Natural Aging Rats Disease Markers 2022-08-21 [PMID: 36046381] (WB, Rat) WB Rat
Zhang Z, Tan Q, Guo P et al. NLRP3 inflammasome-mediated choroid plexus hypersecretion contributes to hydrocephalus after intraventricular hemorrhage via phosphorylated NKCC1 channels Journal of neuroinflammation 2022-06-21 [PMID: 35729645] (WB, Rat) WB Rat
Wu X, Chen Y, Liu C et al. STING Induces Liver Ischemia-Reperfusion Injury by Promoting Calcium-Dependent Caspase 1-GSDMD Processing in Macrophages Oxidative Medicine and Cellular Longevity 2022-01-30 [PMID: 35281468] (IF/IHC, Mouse) IF/IHC Mouse
Sousa AH, Do Vale GT, Nascimento JA Et al. Inhibition of iNOS protects against the deleterious effects of sub-lethal sepsis and ethanol in the cardiorenal system Canadian journal of physiology and pharmacology 2021-07-27 [PMID: 34314655]
Cau SB, Bruder-Nascimento A, Silva MB et al. Angiotensin-II activates vascular inflammasome and induces vascular damage Vascular pharmacology 2021-06-09 [PMID: 34098096]
El-Rous MA, Saber S, Raafat EM, Ahmed AAE Dapagliflozin, an SGLT2 inhibitor, ameliorates acetic acid-induced colitis in rats by targeting NF kappa B/AMPK/NLRP3 axis Inflammopharmacology 2021-05-17 [PMID: 34002329]
Show All 63 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-1 Antibody (NB100-56565). (Showing 1 - 2 of 2 FAQs).

  1. We have purchased "NB100-56565 Caspase-1 Antibody (14F468)" for our experiments. It is mentioned in product data sheet that it can detect the cleave forms of Caspase-1 as well. Can you kindly tell me the expected size of cleaved subforms on WB, so that I could be sure about my results?
    • The antibody will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein. In short this antibody can recognize any protein species, after processed by protease in vivo, that contains the region of a.a. 371 - 390.
  2. We are interested in your items, NB100-56565 and NB100-56564 and I would like to use them for recognizing the cleaved form of Caspase-1. Would you please let me know if NB100-56565 and NB100-56564 recognize the Caspase-1 p10 or p20? If so, could you please provide me any supporting data of them?
    • For NB100-56565 & NB100-56564 it will recognize the full length form and the p10 as the immunogen. A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen. The p10 subunit is aa 307-404. If you need an antibody to the p20 subunit: This will bind to the the region around aa 132. The p20 subunit is aa 120-297.

Control Lysate(s)

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Isotype Controls

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Blogs on Caspase-1.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

The inflammasome: an inflammation-initiating machine, Novus Biologicals
By Stephanie Melchor The inflammasome is a large, multimeric protein complex found primarily in innate immune cells, which are white blood cells that can attack a wide range of pathogenic threats. Three main elements ...  Read full blog post.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

Caspase 10 - an initiator caspase in the extrinsic death receptor pathway
Apoptosis, also called programmed cell death, is an essential process in development and disease. The signaling networks that carry out apoptosis is consists of a series of endoproteases called caspases which are synthesized as inactive zymogens. ...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 1 - activating innate immune responses following infection or injury
Caspase-1 is an enzyme involved in the conversion of interleukin-1 into its active secreted form. Interleukin-1 mediates inflammatory responses during infection and disease. Caspase-1 is recruited to and activated by the inflammasome complex (1). Un...  Read full blog post.

Caspase-4 - a human protease with roles in inflammation and immunity
Caspases are a family of cysteine-aspartic acid proteases that cleave caspase proenzymes as well as other protein substrates. Caspases are well known for their role in apoptosis, but they also play a significant role in other cellular processes inc...  Read full blog post.

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Gene Symbol CASP1