ASC/TMS1 Antibody

Images

 
Western Blot: ASC/TMS1 Antibody [NBP1-78977] - Whole cell protein from (1) THP1 and (2) HL60 was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - Tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Tested in mouse intestine at a 1:400 dilution.
Simple Western: ASC/TMS1 Antibody [NBP1-78977] - Lane view shows a specific band for ASC TMS1 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.19 mg/ml

ASC/TMS1 Antibody Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human ASC/TMS1 protein (between residues 1-50) [Uniprot: Q9ULZ3]
Localization
Cytoplasm. Note: Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PYCARD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0 - 4.0 ug/ml
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence 1:40-1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-78977 in the following applications:

Publications
Read Publications using
NBP1-78977 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Reactivity with Rat reported in PMID 24464748

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.19 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASC/TMS1 Antibody

  • ASC
  • ASCMGC10332
  • CARD5
  • CARD5TMS
  • caspase recruitment domain protein 5
  • Caspase recruitment domain-containing protein 5
  • hASC
  • PYCARD
  • PYD and CARD domain containing
  • PYD and CARD domain-containing protein
  • Target of methylation-induced silencing 1
  • TMS1
  • TMS-1
  • TMS1apoptosis-associated speck-like protein containing a CARD

Background

Target of methylation-induced silencing 1 protein, TMS1 (ASC, Pycard) is a 21 kDa cytoplasmic adaptor protein containing 2 protein-protein interaction domains: an N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). Both domains are members of the six-helix bundle death domain superfamily. These domains, via caspase activation, mediate the assembly of large signaling complexes that are involved in the apoptotic and inflammatory signaling pathways. During apoptosis TMS1 aggregates near the nuclear periphery as ball-like structures. Recent research suggests that it can modulate MAPK expression by suppressing the DUSP10/MKP5 phosphatase thus allowing the phosphorylation of MAPK by pathogens and toll-like receptor agonists. PMID: 2148701.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for ASC/TMS1 Antibody (NBP1-78977)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(3)
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Human
(1)
Mouse
(4)
Rat
(1)
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Showing Publications 1 - 9 of 9.
Publications using NBP1-78977 Applications Species
Wang X, Pan J, Liu H et al. AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model Life Sci. Feb 18 2019 [PMID: 30790610] (WB, Rat) WB Rat
Kariya S, Okano M, Zhao P et al. NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media. Acta Otolaryngol. Jan 24 2019 [PMID: 30676848] (IHC-P, Mouse) IHC-P Mouse
Zhou L, Su X, Li B et al. PM2.5 exposure impairs sperm quality through testicular damage dependent on NALP3 inflammasome and miR-183/96/182 cluster targeting FOXO1 in mouse. Sci Rep. Nov 19 2018 [PMID: 30476817] (WB, Mouse) WB Mouse
Cohen TS, Boland ML, Boland BB et al. S. aureus Evades Macrophage Killing through NLRP3-Dependent Effects on Mitochondrial Trafficking. Cell Rep. Feb 27 2018 [PMID: 29490278] (Human) Human
Liang Y, Liang S, Zhang Y et al. Oral Administration of Compound Probiotics Ameliorates HFD-Induced Gut Microbe Dysbiosis and Chronic Metabolic Inflammation via the G Protein-Coupled Receptor 43 in Non-alcoholic Fatty Liver Disease Rats. Probiotics Antimicrob Proteins 2018 Jan 20 [PMID: 29353414]
Zhang B, Xu D, She L et al. Silybin inhibits NLRP3 inflammasome assembly through the NAD(+)/SIRT2 pathway in mice with nonalcoholic fatty liver disease. FASEB J. 2017 Sep 27 [PMID: 28970254] (Mouse) Mouse
Kim SJ, Cha JY, Kang HS et al. Corosolic acid ameliorates acute inflammation through inhibition of IRAK-1 phosphorylation in macrophages. BMB Rep. May 1 2016 [PMID: 26615974] (Mouse) Mouse
Kariya S, Okano M, Zhao P et al. Activation of NLRP3 inflammasome in human middle ear cholesteatoma and chronic otitis media. Acta Otolaryngol 2015 Oct 12 [PMID: 26457439]
Yao X, Carlson D, Sun Y et al. Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model. PLoS One 2015 [PMID: 26448624] (WB) WB

Reviews for ASC/TMS1 Antibody (NBP1-78977) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP1-78977:
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WB
(2)
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Mouse
(1)
Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot ASC/TMS1 NBP1-78977
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reviewed by:
wang xuyang
WB Rat 12/20/2017
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Summary

ApplicationWestern Blot
Sample TestedH9c2 whole cell lysate
SpeciesRat
Lotlot A-4
Western Blot ASC/TMS1 NBP1-78977
Enlarge
reviewed by:
Anonymous
WB Mouse 05/08/2016
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Summary

ApplicationWestern Blot
Sample TestedMouse macrophages
SpeciesMouse

Blocking

Blocking Details5% Skim milk

Primary Anitbody

Dilution Ratio1:1000, 24h (overnight), 4 deg, 5% Skim milk in TBS + 0.1% Tween 20

Secondary Antibody

Secondary DescriptionPeroxidase Goat anti-rabbit IgG
Secondary Concentration1:1000

Details

Detection NotesChemoluminiscence, FluorChem HD2 equipment, 1-2 min exposure, negative control tested using IHC

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ASC/TMS1 Antibody (NBP1-78977) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-78977AF405
Alexa Fluor 488 Labeled NBP1-78977AF488
Alexa Fluor 647 Labeled NBP1-78977AF647
Alexa Fluor 700 Labeled NBP1-78977AF700
Biotin Labeled NBP1-78977B
DyLight 405 Labeled NBP1-78977V
DyLight 488 Labeled NBP1-78977G
DyLight 550 Labeled NBP1-78977R
DyLight 650 Labeled NBP1-78977C
FITC Labeled NBP1-78977F
HRP Labeled NBP1-78977H

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Bioinformatics Tool for ASC/TMS1 Antibody (NBP1-78977)

Discover related pathways, diseases and genes to ASC/TMS1 Antibody (NBP1-78977). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for ASC/TMS1 Antibody (NBP1-78977)

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Blogs on ASC/TMS1.

The inflammasome: an inflammation-initiating machine, Novus Biologicals
By Stephanie Melchor The inflammasome is a large, multimeric protein complex found primarily in innate immune cells, which are white blood cells that can attack a wide range of pathogenic threats. Three main elements...  Read full blog post.

Immunity’s flipside: Microglia promote Alzheimer’s pathology during inflammation
By Jamshed Arslan Pharm.D. Microglia are brain's macrophages. In Alzheimer's disease (AD), microglia clear up protein aggregates called amyloid beta plaques. The connection between immune activation and AD is unclea...  Read full blog post.

CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

wang xuyang
12/20/2017
Application: WB
Species: Rat

Anonymous
05/08/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PYCARD
Entrez
Uniprot