ASC/TMS1 Antibody

Images

 
Simple Western: ASC/TMS1 Antibody [NBP1-78977] - Lane view shows a specific band for ASC TMS1 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Biological Strategies: Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - NLRP3 activation was evaluated by visualizing the co-localization of NLRP3 and apoptosis-associated speck-like ...read more
Biological Strategies: Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Rats were infected by S. pneumoniae or given PBS sham control. 21.5 umoles/kg Mito-Vit-E (MVE) or vehicle was administered orally 30 ...read more
Western Blot: ASC/TMS1 Antibody [NBP1-78977] - Whole cell protein from (1) THP1 and (2) HL60 was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - Tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Tested in mouse intestine at a 1:400 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IM, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

ASC/TMS1 Antibody Summary

Immunogen
This ASC/TMS1 Antibody was developed against a synthetic peptide made to an N-terminal portion of the human ASC/TMS1 protein (between residues 1-50) [Uniprot: Q9ULZ3]
Localization
Cytoplasm. Note: Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PYCARD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0 - 4.0 ug/ml
  • Simple Western 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:40-1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunomicroscopy
  • Immunoprecipitation
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Immunomicroscopy reported in scientific literature (PMID: 31054188). Use in FLOW reported in scientific literature (PMID: 31214205). Use in immunoprecipitation reported in scientific literature (PMID:31551961).
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-78977 in the following applications:

Publications
Read Publications using
NBP1-78977 in the following applications:

  • 1 publication
  • 2 publications
  • 3 publications
  • IM
    1 publication
  • IP
    2 publications
  • WB
    7 publications

Reactivity Notes

Reactivity with Rat reported in PMID 24464748

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ASC/TMS1 Antibody

  • apoptosis-associated speck-like protein containing a CARD
  • ASC
  • CARD5
  • caspase recruitment domain protein 5
  • Caspase recruitment domain-containing protein 5
  • hASC
  • PYCARD
  • PYD and CARD domain-containing protein
  • Target of methylation-induced silencing 1
  • TMS
  • TMS1
  • TMS-1

Background

ASC (apoptosis-associated speck-like protein containing a CARD), also known as TMS1 (target of methylation-induced silencing), was first identified in 1999 as a protein that forms aggregates, or specks, during retinoic acid-induced apoptosis in a human leukemia cell line (1). Furthermore, it was discovered to have a role as a tumor suppressor as methylation silences ASC/TMS1 expression in many tumors (2-5). ASC/TMS1 is synthesized as a 195-amino acid (aa) protein with a theoretical weight of 22 kDa. Structurally the protein contains a N-terminal PYD (pyrin domain) and C-terminal CARD (caspase-recruitment domain) (1-4). Historically, CARD and PYD-containing proteins are known to have crucial functions in regulating apoptosis and immune response pathways (2-5). Furthermore, mutations in many CARD and PYD-containing proteins have been linked to various cancers and inflammatory diseases (2-5). Given its immune response role, it is not surprising that ASC/TMS1 is typically highly expressed in immune cells, specifically in neutrophils and cells of the macrophage/monocyte lineage (5). Additionally, it is expressed in many normal epithelial cell types (4,5).

In regard to immune and inflammatory response, ASC/TMS1 is involved in inflammasome function (3-4). The inflammasome is a multiprotein complex that responds to cellular stress or pathogens and activates inflammatory responses. Specifically, ASC/TMS1 helps assemble the NLRP3 inflammasome complex which then activates caspase-1, followed by stimulation of proinflammatory cytokines including IL-1b and IL-18 (3-4). In terms of the role in regulating apoptosis, multiple studies have revealed that the ASC/TMS1 gene is hypermethylated in many cancers including breast, lung, glioblastomas, and melanomas (2-5). The increased methylation results in decreased gene expression, or silencing, allowing those cancer cells to escape apoptosis (2-5).

References

1. Masumoto, J., Taniguchi, S., Ayukawa, K., Sarvotham, H., Kishino, T., Niikawa, N., Hidaka, E., Katsuyama, T., Higuchi, T., & Sagara, J. (1999). ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. The Journal of biological chemistry, 274(48), 33835-33838. https://doi.org/10.1074/jbc.274.48.33835

2. McConnell, B. B., & Vertino, P. M. (2004). TMS1/ASC: the cancer connection. Apoptosis: an international journal on programmed cell death. https://doi.org/10.1023/B:APPT.0000012117.32430.0c

3. Salminen, A., Kauppinen, A., Hiltunen, M., & Kaarniranta, K. (2014). Epigenetic regulation of ASC/TMS1 expression: potential role in apoptosis and inflammasome function. Cellular and molecular life sciences : CMLS. https://doi.org/10.1007/s00018-013-1524-9

4. Protti, M. P., & De Monte, L. (2020). Dual Role of Inflammasome Adaptor ASC in Cancer. Frontiers in cell and developmental biology. https://doi.org/10.3389/fcell.2020.00040

5. Parsons, M. J., & Vertino, P. M. (2006). Dual role of TMS1/ASC in death receptor signaling. Oncogene. https://doi.org/10.1038/sj.onc.1209684

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASC/TMS1 Antibody (NBP1-78977)(21)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Flow, ICC/IF, IHC-P, IM, IP, WB.


Filter By Application
Flow
(1)
ICC/IF
(2)
IHC-P
(3)
IM
(1)
IP
(2)
WB
(7)
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Human
(6)
Mouse
(6)
Rat
(5)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NBP1-78977 Applications Species
Zhang XL, Huang WM, Tang PC et al. Anti-inflammatory and neuroprotective effects of natural cordycepin in rotenone-induced PD models through inhibiting Drp1-mediated mitochondrial fission Neurotoxicology Feb 4 2021 [PMID: 33549657] (WB, Rat) WB Rat
Gordon M, Meagher P, Connelly K The Effect of Empagliflozin and Liraglutide on the NLRP3 Inflammasome in a rodent model of type 2 Diabetes Mellitus Canadian Journal of Diabetes Nov 1 2020 [PMID: 33388277] (WB, Mouse) WB Mouse
Verma V, Kumar P, Gupta S et al. Alpha-Hemolysin of uropathogenic E. coli regulates NLRP3 inflammasome activation and mitochondrial dysfunction in THP-1 macrophages Sci Rep Jul 28 2020 [PMID: 32724079] (IP, WB, Human) IP, WB Human
Cornelius DC, Travis OK, Tramel RW et al. NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture PLoS ONE Jun 17 2020 [PMID: 32555710] (ICC/IF, Rat)

Details:
Citation using the Alexa Fluor 647 format of this antibody.
ICC/IF Rat
Perez-Martinez PI, Rojas-Espinosa O, Hernandez-Chavez VG et al. Anti-inflammatory effect of omega unsaturated fatty acids and dialysable leucocyte extracts on collagen-induced arthritis in DBA/1 mice Int J Exp Pathol May 28 2020 [PMID: 32459025] (IHC-P, Mouse) IHC-P Mouse
Dang S, Zhang Zy, Li Kl et Al. Blockade of beta-adrenergic signaling suppresses inflammasome and alleviates cardiac fibrosis Ann Transl Med Feb 1 2020 [PMID: 32175420] (WB, Human) WB Human
Verma V, Gupta S, Kumar P et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections Front Microbiol [PMID: 31551961] (IP, Human) IP Human
Lage SL, Dominical VM, Wong CS, Sereti I Evaluation of Canonical Inflammasome Activation in Human Monocytes by Imaging Flow Cytometry Thesis Jun 4 2019 [PMID: 31214205] (Flow, Human) Evaluation of Canonical Inflammasome Activation in Human Monocytes by Imaging Flow Cytometry. Front Immunol. 2019 Jun 4 [PMID: 31214205] (Flow, Human)

Details:
Citation using the Alexa Fluor 647 version of this antibody.
Flow Human
Cornelius DC, Baik CH, Travis OK, et al. NLRP3 inflammasome activation in platelets in response to sepsis. Physiol Rep. 2019 May 03 [PMID: 31054188] (IM, Rat) IM Rat
Wang X, Pan J, Liu H et al. AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model Life Sci. Feb 18 2019 [PMID: 30790610] (WB, Rat) WB Rat
Show All 21 Publications.

Reviews for ASC/TMS1 Antibody (NBP1-78977) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP1-78977:
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WB
(2)
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(1)
Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot ASC/TMS1 NBP1-78977
Enlarge
5
reviewed by:
wang xuyang
WB Rat 12/20/2017
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Summary

ApplicationWestern Blot
Sample TestedH9c2 whole cell lysate
SpeciesRat
Lotlot A-4
Western Blot ASC/TMS1 NBP1-78977
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 05/08/2016
View

Summary

ApplicationWestern Blot
Sample TestedMouse macrophages
SpeciesMouse

Blocking

Blocking Details5% Skim milk

Primary Anitbody

Dilution Ratio1:1000, 24h (overnight), 4 deg, 5% Skim milk in TBS + 0.1% Tween 20

Secondary Antibody

Secondary DescriptionPeroxidase Goat anti-rabbit IgG
Secondary Concentration1:1000

Details

Detection NotesChemoluminiscence, FluorChem HD2 equipment, 1-2 min exposure, negative control tested using IHC

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ASC/TMS1 Antibody (NBP1-78977) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-78977AF350
Alexa Fluor 405 NBP1-78977AF405
Alexa Fluor 488 NBP1-78977AF488
Alexa Fluor 532 NBP1-78977AF532
Alexa Fluor 594 NBP1-78977AF594
Alexa Fluor 647 NBP1-78977AF647
Alexa Fluor 700 NBP1-78977AF700
Alexa Fluor 750 NBP1-78977AF750
Allophycocyanin NBP1-78977APC
Biotin NBP1-78977B
DyLight 350 NBP1-78977UV
DyLight 405 NBP1-78977V
DyLight 488 NBP1-78977G
DyLight 550 NBP1-78977R
DyLight 594 NBP1-78977DL594
DyLight 650 NBP1-78977C
DyLight 680 NBP1-78977FR
DyLight 755 NBP1-78977IR
FITC NBP1-78977F
HRP NBP1-78977H
Janelia Fluor 549 NBP1-78977JF549
Janelia Fluor 646 NBP1-78977JF646
PerCP NBP1-78977PCP

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Blogs on ASC/TMS1.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

The inflammasome: an inflammation-initiating machine, Novus Biologicals
By Stephanie Melchor The inflammasome is a large, multimeric protein complex found primarily in innate immune cells, which are white blood cells that can attack a wide range of pathogenic threats. Three main elements...  Read full blog post.

Immunity’s flipside: Microglia promote Alzheimer’s pathology during inflammation
By Jamshed Arslan Pharm.D. Microglia are brain's macrophages. In Alzheimer's disease (AD), microglia clear up protein aggregates called amyloid beta plaques. The connection between immune activation and AD is unclea...  Read full blog post.

CARD & NFKB Antibodies for Apoptosis Research
Apoptosis is one of the main types of programmed cell death which involves a cascade of biochemical events leading to specific cell morphology characteristics and ultimately death of cells.Caspases play crucial roles in modulating cellular signaling...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

wang xuyang
12/20/2017
Application: WB
Species: Rat

Verified Customer
05/08/2016
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PYCARD
Entrez
Uniprot