Aquaporin-2 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts. Cortex (CX), Outer Medulla (OM), Inner Medulla Base (IMB), Inner Medulla Papilla (IMP).
Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NB110-74682] - Analysis of FFPE human kidney using 1:100 conc. of Aquaporin-2 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of ...read more
Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - Staining of mpkCCD cell line

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Aquaporin-2 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to a C-terminus portion of rat Aquaporin-2 (within residues 200-300). [Swiss-Prot# P34080]
Localization
Cell membrane
Predicted Species
Porcine (100%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AQP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1 ug/ml
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen reported in scientific literature
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1 - 2 ug/ml
Application Notes
Bands can be seen ~28 kDa for the non-glycosylated form and ~37 kDa for the glycosylated form in Western Blot.
Publications
Read Publications using
NB110-74682 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33634832).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aquaporin-2 Antibody - BSA Free

  • ADH water channel
  • AQP-2
  • AQP-CD
  • aquaporin 2 (collecting duct)
  • aquaporin-2
  • aquaporin-CD
  • Collecting duct water channel protein
  • MGC34501
  • Water channel protein for renal collecting duct
  • water-channel aquaporin 2
  • WCH-CD

Background

Aquaporin 2 (AQP2) is a membrane protein involved in plasma membrane water transport in the renal collecting duct. This antibody is targeted to a peptide sequence upstream from the phosphorylated region that, upon phosphorylation, can diminish the antibody signal in immunoblots and immunocytochemistry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aquaporin-2 Antibody (NB110-74682)(32)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Camel, Porcine.

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(9)
IF/IHC
(4)
IHC
(1)
IHC-Fr
(4)
IHC-P
(5)
WB
(10)
All Applications
Filter By Species
Human
(5)
Mouse
(16)
Rat
(3)
Bovine
(1)
Camel
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB110-74682 Applications Species
Micha?ek K, Oberska P, Murawski M et al. Kidney morphology and renal expression of aquaporins 2, 3 and 4 during cerulein - Induced chronic pancreatitis in pigs Advances in medical sciences 2023-09-12 [PMID: 37708639] (IHC-Fr, WB, Porcine) IHC-Fr, WB Porcine
Breiderhoff T, Himmerkus N, Meoli L et al. Claudin-10a Deficiency Shifts Proximal Tubular Cl- Permeability to Cation Selectivity via Claudin-2 Redistribution Journal of the American Society of Nephrology : JASN 2022-01-14 [PMID: 35031570]
Dixon EE, Wu H, Muto Y et al. Spatially Resolved Transcriptomic Analysis of Acute Kidney Injury in a Female Murine Model Journal of the American Society of Nephrology : JASN 2021-12-01 [PMID: 34853151] (IHC-Fr, Mouse) IHC-Fr Mouse
Grabowska M, Michalek K, Kedzierska-Kapuza K et al. The long-term effects of rapamycin-based immunosuppressive protocols on the expression of renal aquaporins 1, 2, 3 and 4 water channels in rats Histology and histopathology 2021-02-26 [PMID: 33634832] (IHC-P, Rat) IHC-P Rat
Uchimura K, Wu H, Yoshimura Y, Humphreys BD Human Pluripotent Stem Cell-Derived Kidney Organoids with Improved Collecting Duct Maturation and Injury Modeling Cell reports 2020-12-15 [PMID: 33326782]
Li B, Zhu C, Dong L et al. ADAM10 mediates ectopic proximal tubule development and renal fibrosis through Notch signalling J. Pathol. 2020-07-27 [PMID: 32715474] (IHC-P, Human) IHC-P Human
Bezerra Duarte D, Cavalcante Meneses G, Bandeira Lima D et al. Aquaporin-2 and NKCC2 expression pattern in patients with hepatosplenic schistosomiasis Trop. Med. Int. Health 2020-07-06 [PMID: 32632995]
Nordstrom CK, Danckwardt-Lilliestrom N, Liu W, Rask-Andersen H Reversed polarizationof Na/K-ATPase-a sign of inverted transport in the human endolymphatic sac: a super-resolution structured illumination microscopy (SR-SIM) study Cell Tissue Res. 2019-11-12 [PMID: 31713726]
Li ZL, Lv LL, Wang B et al. The profibrotic effects of MK-8617 on tubulointerstitial fibrosis mediated by the KLF5 regulating pathway FASEB J. 2019-08-26 [PMID: 31451021] (Mouse) Mouse
Shi M, Luo W, Feng X et al. Urinary Angiotensinogen Predicts Renal Disease Activity in Lupus Nephritis Antioxid. Redox Signal. 2019-07-26 [PMID: 31264479]
Show All 32 Publications.

Reviews for Aquaporin-2 Antibody (NB110-74682) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aquaporin-2 Antibody (NB110-74682). (Showing 1 - 2 of 2 FAQs).

  1. We have a customer who is interested in catalog # NB110-74682, but he needs more information regarding the sequence if possible. The range of 200-300 covers both a predicted helical and cytoplasmic domain. Would it be possible to know if the exact sequence is contained entirely within either of these domains? He is hoping for the helical as he needs to detect on the cell surface.
    • Unfortunately for your customer, NB110-74682 recognizes the cytoplasmic domain.
  2. I have a customer who is interested in antibody NB100-74682 anti-Aquaporin. They are concerned about the fact that there are only a few extracellular amino acids in the region 200-300 of AQP2. Please see below. The immunogen for anti-AQP2 is listed as aa 200 - 300 of aquaporin 2. This product is listed as having application in ICC, which requires an extracellular epitope. From looking at the structure of Aquaporin 2, it appears that only about 4 residues of aa200 - 300 are extracellular. Can you explain this? Do you know if the cells were permeabilized for the ICC image shown on your datasheet or was the antibody able to cross the membrane efficiently enough to stain without permeabilizing the cells?
    • Our immunogen for NB110-74682 is to the cytoplasmic domain of AQP2. Furthermore, your customer is incorrect that ICC requires and extracellular domain, as many antibodies that recognize an intracellular target work quite well for ICC when the cells are permeabilized. We permeabilize for all of our ICC testing and this antibody certainly requires it.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AQP2
Entrez
Uniprot