Aquaporin-2 Antibody

Images

 
Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts Key: CX=Cortex, OM=Outer Medulla, IMB =Inner Medulla Base, IMP = Inner Medulla Papilla
Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - Staining of mpkCCD cell line

Product Details

Summary
Reactivity Hu, Mu, Rt, Bovine, Porcine, SheepSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
0.053 mg/ml

Order Details

Aquaporin-2 Antibody Summary

Immunogen
Synthetic peptide made to a C-terminus portion of rat Aquaporin-2 (within residues 200-300). [Swiss-Prot# P34080]
Localization
Cell membrane
Predicted Species
Porcine (100%), Sheep (100%), Bovine (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
AQP2
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.053 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
This Aquaporin-2 antibody is useful for Western blot, where bands can be seen ~28 kDa for the non-glycosylated form and ~37 kDa for the glycosylated form. It is also useful for Immunocytochemistry and Immunohistochemistry on paraffin-embedded sections. Immunohistochemistry-Frozen was reported in scientific literature.
Publications
Read Publications using
NB110-74682 in the following applications:

Reactivity Notes

Human, rat and mouse. 100% sequence identity with sheep, porcine, and bovine proteins.

Alternate Names for Aquaporin-2 Antibody

  • ADH water channel
  • AQP-2
  • AQP-CD
  • aquaporin 2 (collecting duct)
  • aquaporin-2
  • aquaporin-CD
  • Collecting duct water channel protein
  • MGC34501
  • Water channel protein for renal collecting duct
  • water-channel aquaporin 2
  • WCH-CD

Background

Aquaporin 2 (AQP2) is a membrane protein involved in plasma membrane water transport in the renal collecting duct. This antibody is targeted to a peptide sequence upstream from the phosphorylated region that, upon phosphorylation, can diminish the antibody signal in immunoblots and immunocytochemistry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aquaporin-2 Antibody (NB110-74682)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(5)
IHC
(4)
IHC-Fr
(2)
IHC-P
(1)
WB
(8)
All Applications
Filter By Species
Human
(1)
Mouse
(11)
Rat
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB110-74682 Applications Species
Martins JR, Penton D, Peyronnet R et al. Piezo1-dependent regulation of urinary osmolarity. Pflugers Arch. 2016 Mar 29 [PMID:27023350] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Yang CR, Raghuram V, Emamian M et al. Deep Proteomic Profiling of Vasopressin-Sensitive Collecting Duct Cells. II. Bioinformatic Analysis of Vasopressin Signaling. Am. J. Physiol., Cell Physiol. 2015 Aug 26 [PMID:26310817] (WB, Mouse) WB Mouse
Gilbert ML, Yang L, Su T, McKnight GS. Expression of a dominant negative Protein Kinase A (PKA) mutation in kidney elicits a diabetes insipidus phenotype Am. J. Physiol. Renal Physiol. 2015 Jan 13 [PMID:25587115] (WB, IHC, Mouse) WB, IHC Mouse
Sato E, Nakamura T, Amaha M et al. Effect of Tolvaptan in Patients With Chronic Kidney Disease due to Diabetic nephropathy With Heart Failure. Int Heart J. 2014 Oct 16 [PMID:25318553] (IHC-P, Human) IHC-P Human
Gao Y, Romero-Aleshire MJ, Cai Q et al. Rapamycin inhibition of mTORC1 reverses lithium-induced proliferation of renal collecting duct cells. Am J Physiol Renal Physiol. 2013 Oct [PMID:23884148] (WB, Mouse) WB Mouse
Sanz E, Evanoff R, Quintana A et al. RiboTag Analysis of Actively Translated mRNAs in Sertoli and Leydig Cells In Vivo. PLoS One 2013 Jun 11 [PMID:23776628] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Takumida M, Takumida H, Kakigi A et al. Localization of aquaporins in the mouse vestibular end organs. Acta Otolaryngol 2013 Apr 30 [PMID:23628076] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Toka HR, Al-Romaih K, Koshy JM et al. Deficiency of the Calcium-Sensing Receptor in the Kidney Causes Parathyroid Hormone-Independent Hypocalciuria J Am Soc Nephrol 2012 Sep 20 [PMID:22997254]
Takumida M, Kakigi A, Egami N et al. Localization of aquaporins 1, 2, and 3 and vasopressin type 2 receptor in the mouse inner ear Acta Otolaryngol 2012 Jul 6 [PMID:22768909] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Stegbauer J, Gurley SB, Sparks MA et al. AT1 receptors in the collecting duct directly modulate the concentration of urine. J Am Soc Nephrol 2011 Dec [PMID:22052052] (Mouse) Mouse
Show All 14 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aquaporin-2 Antibody (NB110-74682). (Showing 1 - 2 of 2 FAQs).

  1. We have a customer who is interested in catalog # NB110-74682, but he needs more information regarding the sequence if possible. The range of 200-300 covers both a predicted helical and cytoplasmic domain. Would it be possible to know if the exact sequence is contained entirely within either of these domains? He is hoping for the helical as he needs to detect on the cell surface.
    • Unfortunately for your customer, NB110-74682 recognizes the cytoplasmic domain.
  2. I have a customer who is interested in antibody NB100-74682 anti-Aquaporin. They are concerned about the fact that there are only a few extracellular amino acids in the region 200-300 of AQP2. Please see below. The immunogen for anti-AQP2 is listed as aa 200 - 300 of aquaporin 2. This product is listed as having application in ICC, which requires an extracellular epitope. From looking at the structure of Aquaporin 2, it appears that only about 4 residues of aa200 - 300 are extracellular. Can you explain this? Do you know if the cells were permeabilized for the ICC image shown on your datasheet or was the antibody able to cross the membrane efficiently enough to stain without permeabilizing the cells?
    • Our immunogen for NB110-74682 is to the cytoplasmic domain of AQP2. Furthermore, your customer is incorrect that ICC requires and extracellular domain, as many antibodies that recognize an intracellular target work quite well for ICC when the cells are permeabilized. We permeabilize for all of our ICC testing and this antibody certainly requires it.

Secondary Antibodies

 

Isotype Controls

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Blogs on Aquaporin-2.

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Heat shock cognate 71 kDa protein (Hsc70), also known as HSPA8, is a member of the heat shock protein 70 family (Hsp70). Unlike Hsp70, it is a constitutively expressed chaperone protein and is involved in diverse cellular processes including protei...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol AQP2
Entrez
Uniprot

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