Aquaporin-2 Antibody


Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts Key: CX=Cortex, OM=Outer Medulla, IMB =Inner Medulla Base, IMP = Inner Medulla Papilla
Immunocytochemistry/ Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - Staining of mpkCCD cell line

Product Details

Reactivity Hu, Mu, Rt, Bovine, Porcine, SheepSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
0.053 mg/ml

Order Details

Aquaporin-2 Antibody Summary

Synthetic peptide made to a C-terminus portion of rat Aquaporin-2 (within residues 200-300). [Swiss-Prot# P34080]
Cell membrane
Predicted Species
Porcine (100%), Sheep (100%), Bovine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.05% Sodium Azide
0.053 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
This Aquaporin-2 antibody is useful for Western blot, where bands can be seen ~28 kDa for the non-glycosylated form and ~37 kDa for the glycosylated form. It is also useful for Immunocytochemistry and Immunohistochemistry on paraffin-embedded sections. Immunohistochemistry-Frozen was reported in scientific literature.
Read Publications using
NB110-74682 in the following applications:

Reactivity Notes

Human, rat and mouse. 100% sequence identity with sheep, porcine, and bovine proteins.

Alternate Names for Aquaporin-2 Antibody

  • ADH water channel
  • AQP-2
  • AQP-CD
  • aquaporin 2 (collecting duct)
  • aquaporin-2
  • aquaporin-CD
  • Collecting duct water channel protein
  • MGC34501
  • Water channel protein for renal collecting duct
  • water-channel aquaporin 2
  • WCH-CD


Aquaporin 2 (AQP2) is a membrane protein involved in plasma membrane water transport in the renal collecting duct. This antibody is targeted to a peptide sequence upstream from the phosphorylated region that, upon phosphorylation, can diminish the antibody signal in immunoblots and immunocytochemistry.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB
Species: Hu, Mu, Rt, Rb, Ze
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, Flow
Species: Hu, Mu, Rt, Pm
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Ha, Mk, Rb
Applications: IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr

Publications for Aquaporin-2 Antibody (NB110-74682)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB110-74682 Applications Species
Martins JR, Penton D, Peyronnet R et al. Piezo1-dependent regulation of urinary osmolarity. Pflugers Arch. 2016 Mar 29 [PMID:27023350] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Yang CR, Raghuram V, Emamian M et al. Deep Proteomic Profiling of Vasopressin-Sensitive Collecting Duct Cells. II. Bioinformatic Analysis of Vasopressin Signaling. Am. J. Physiol., Cell Physiol. 2015 Aug 26 [PMID:26310817] (WB, Mouse) WB Mouse
Gilbert ML, Yang L, Su T, McKnight GS. Expression of a dominant negative Protein Kinase A (PKA) mutation in kidney elicits a diabetes insipidus phenotype Am. J. Physiol. Renal Physiol. 2015 Jan 13 [PMID:25587115] (WB, IHC, Mouse) WB, IHC Mouse
Sato E, Nakamura T, Amaha M et al. Effect of Tolvaptan in Patients With Chronic Kidney Disease due to Diabetic nephropathy With Heart Failure. Int Heart J. 2014 Oct 16 [PMID:25318553] (IHC-P, Human) IHC-P Human
Gao Y, Romero-Aleshire MJ, Cai Q et al. Rapamycin inhibition of mTORC1 reverses lithium-induced proliferation of renal collecting duct cells. Am J Physiol Renal Physiol. 2013 Oct [PMID:23884148] (WB, Mouse) WB Mouse
Sanz E, Evanoff R, Quintana A et al. RiboTag Analysis of Actively Translated mRNAs in Sertoli and Leydig Cells In Vivo. PLoS One 2013 Jun 11 [PMID:23776628] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Takumida M, Takumida H, Kakigi A et al. Localization of aquaporins in the mouse vestibular end organs. Acta Otolaryngol 2013 Apr 30 [PMID:23628076] (IHC-Fr, ICC/IF, Mouse) IHC-Fr, ICC/IF Mouse
Toka HR, Al-Romaih K, Koshy JM et al. Deficiency of the Calcium-Sensing Receptor in the Kidney Causes Parathyroid Hormone-Independent Hypocalciuria J Am Soc Nephrol 2012 Sep 20 [PMID:22997254]
Takumida M, Kakigi A, Egami N et al. Localization of aquaporins 1, 2, and 3 and vasopressin type 2 receptor in the mouse inner ear Acta Otolaryngol 2012 Jul 6 [PMID:22768909] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Stegbauer J, Gurley SB, Sparks MA et al. AT1 receptors in the collecting duct directly modulate the concentration of urine. J Am Soc Nephrol 2011 Dec [PMID:22052052] (Mouse) Mouse
Show All 14 Publications.

Reviews for Aquaporin-2 Antibody (NB110-74682) (0)

There are no reviews for Aquaporin-2 Antibody (NB110-74682). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aquaporin-2 Antibody (NB110-74682). (Showing 1 - 2 of 2 FAQs).

  1. We have a customer who is interested in catalog # NB110-74682, but he needs more information regarding the sequence if possible. The range of 200-300 covers both a predicted helical and cytoplasmic domain. Would it be possible to know if the exact sequence is contained entirely within either of these domains? He is hoping for the helical as he needs to detect on the cell surface.
    • Unfortunately for your customer, NB110-74682 recognizes the cytoplasmic domain.
  2. I have a customer who is interested in antibody NB100-74682 anti-Aquaporin. They are concerned about the fact that there are only a few extracellular amino acids in the region 200-300 of AQP2. Please see below. The immunogen for anti-AQP2 is listed as aa 200 - 300 of aquaporin 2. This product is listed as having application in ICC, which requires an extracellular epitope. From looking at the structure of Aquaporin 2, it appears that only about 4 residues of aa200 - 300 are extracellular. Can you explain this? Do you know if the cells were permeabilized for the ICC image shown on your datasheet or was the antibody able to cross the membrane efficiently enough to stain without permeabilizing the cells?
    • Our immunogen for NB110-74682 is to the cytoplasmic domain of AQP2. Furthermore, your customer is incorrect that ICC requires and extracellular domain, as many antibodies that recognize an intracellular target work quite well for ICC when the cells are permeabilized. We permeabilize for all of our ICC testing and this antibody certainly requires it.

Secondary Antibodies


Isotype Controls

Additional Aquaporin-2 Antibody Products

Related Products by Gene

Bioinformatics Tool for Aquaporin-2 Antibody (NB110-74682)

Discover related pathways, diseases and genes to Aquaporin-2 Antibody (NB110-74682). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Aquaporin-2 Antibody (NB110-74682)

Discover more about diseases related to Aquaporin-2 Antibody (NB110-74682).

Pathways for Aquaporin-2 Antibody (NB110-74682)

View related products by pathway.

PTMs for Aquaporin-2 Antibody (NB110-74682)

Learn more about PTMs related to Aquaporin-2 Antibody (NB110-74682).

Research Areas for Aquaporin-2 Antibody (NB110-74682)

Find related products by research area.

Blogs on Aquaporin-2.

Hsc70 - a chaperone protein with diverse cellular functions
Heat shock cognate 71 kDa protein (Hsc70), also known as HSPA8, is a member of the heat shock protein 70 family (Hsp70). Unlike Hsp70, it is a constitutively expressed chaperone protein and is involved in diverse cellular processes including protei...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol AQP2

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB110-74682 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought