NLRP3/NALP3 Antibody

Western Blot: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.

Immunocytochemistry: NLRP3/NALP3 Antibody [NBP1-77080] - Staining of NALP3 in K562 cells with NALP3 antibody at 2 ug/mL.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody [NBP1-77080] - K562 cells with NALP3 antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC-P, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

NLRP3/NALP3 Antibody Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3. The immunogen is located within amino acids 120 - 170 of NALP3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 2 ug/mL
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry-Paraffin
  • Immunocytochemistry
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  NLRP3/NALP3 Protein (NBP1-77080PEP)

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine: (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NLRP3/NALP3 Antibody

• AGTAVPRL
• AII
• AII/AVP
• Angiotensin/vasopressin receptor AII/AVP-like
• AVP
• C1orf7
• Caterpiller protein 1.1
• CIAS1
• CLR1.1
• Cold autoinflammatory syndrome 1 protein
• Cold autoinflammatory syndrome 1
• Cryopyrin
• Familial cold autoinflammatory syndrome
• FCAS
• FCU
• Leucine-rich repeat-, and PYD-containing protein 3
• Muckle-Wells syndrome
• MWS
• NACHT
• NACHT, LRR and PYD containing protein 3
• NACHT, LRR and PYD domains-containing protein 3
• NALP3
• NLR family, pyrin domain containing 3
• NLRP3
• Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
• PYPAF1
• PYRIN-containing APAF1-like protein 1

Background

NALP3, a member of Nod-like receptors, has a crucial role in inflammation and immunity and may be a proximal sensor of cellular stress and danger signals. NALP3 forms a caspase-1 activating molecular complex termed the inflammasome. The inflammasome allows activation of IL-1beta, the key player of the inflammation and fever. NALP3 gene encodes a pyrin like protein which contains a pyrin domain, a nucleotide binding site (NBS) domain, and a leucine rich repeat (LRR) motif. NALP3 protein interacts with apoptosis associated speck like protein containing a CARD and may function as an inducer of apoptosis and an activator of NF-kappaB signaling. Defects in NALP3 have been associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP3/NALP3 Antibody (NBP1-77080)(14)

Publications using NBP1-77080

• Geyfman M, Debabov D, Poloso N, Alvandi N. Mechanistic Insight into the Activity of a Sulfone Compound Dapsone on Propionibacterium (Newly Reclassified as Cutibacterium) Acnes-Mediated Cytokine Production Exp. Dermatol. Dec 26 2018 [PMID: 30585659] (WB, Human)
• Pan Z, Shan Q, Gu P, Wang XM. miRNA-23a/CXCR4 regulates neuropathic pain via directly targeting TXNIP/NLRP3 inflammasome axis. J Neuroinflammation. 2018 Jan 31 [PMID: 29386025] (WB, Mouse)
• Zhuang J, Wen X, Zhang YQ et al. TDP-43 upregulation mediated by the NLRP3 inflammasome induces cognitive impairment in 2 2',4,4'-tetrabromodiphenyl ether (BDE-47)-treated mice. Brain Behav. Immun. May 19 2017 [PMID: 28532818]
• Lee HI, Lee SW, Kim NG et al. Low-level light emitting diode (LED) therapy suppresses inflammasome-mediated brain damage in experimental ischemic stroke. J Biophotonics 2017 Feb 06 [PMID: 28164443]
• Zhu W, Cao FS, Feng J et al. NLRP3 inflammasome activation contributes to long-term behavioral alterations in mice injected with lipopolysaccharide. Neuroscience. Dec 5 2016 [PMID: 27923741] (WB, Mouse)
• Lu C, Xu W, Shao J et al. Nrf2 Activation Is Required for Ligustrazine to Inhibit Hepatic Steatosis in Alcohol-Preferring Mice and Hepatocytes Toxicol. Sci Feb 1 2017 [PMID: 27837167] (Mouse)
• Westbom Catherine, Thompson Joyce K, Leggett Alan et al. Inflammasome Modulation by Chemotherapeutics in Malignant Mesothelioma. PloS One 2015 [PMID: 26689911] (WB, Mouse)
• Chi W, Chen H, Li F et al. HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-kB pathway in acute glaucoma. J Neuroinflammation. 2015 Jul 30 [PMID: 26224068] (WB, Mouse)
• Fann DY, Santro T, Manzanero S et al. Intermittent fasting attenuates inflammasome activity in ischemic stroke. Exp. Neurol. 2014 May 05 [PMID: 24805069] (WB, Mouse)
• Yang-Wei Fann D, Lee SY et al. Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke. Cell Death Dis. 2013 Sep 5 [PMID: 24008734] (WB, Mouse)
• Kanneganti TD, Ozoren N, Body-Malapal M et al. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp. Nature; 440:233-6. 2006 [PMID: 16407888]
• Agostini L, Martinon F, Burns K et al. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity; 20:319-25. 2004 [PMID: 15030775]
• O'Connor W Jr, Hartin JA, Zhu X et al. Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties. J. Immunol. 171:6329-33. 2003 [PMID: 14662828]
• Chamaillard M, Girardin SE, Viala J et al. Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation. Cell. Microbiol. 5:581-92. 2003 [PMID: 12925128]

Reviews for NLRP3/NALP3 Antibody (NBP1-77080) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Max MacPherson IHC-P Mouse 11/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse lung
• Species: Mouse
• Lot: A-2

reviewed by: Anonymous IF Mouse 04/03/2015

Summary

• Application: Immunofluorescence
• Sample Tested: corneal epithelium in mouse
• Species: Mouse

Blocking

• Blocking Details: Blocking kit, apply for one hour, in room temperature

Details

• Detection Notes: 20 seconds for exposure

FAQs for NLRP3/NALP3 Antibody (NBP1-77080). (Showing 1 - 4 of 4 FAQs).

1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
   • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
2. Does NLRP3/NALP3 antibodies come in lyophilized form?
   • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
   • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
   • A sequence of 16 amino acides within the N-terminal of human NLRP3/NALP3.

Bioinformatics

• Gene Symbol: NLRP3
• Entrez:
  • 114548(Human)
  • 216799(Mouse)
• Uniprot:
  • Q96P20(Human)