NLRP3/NALP3 Antibody

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.

Western Blot: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody [NBP1-77080] - Staining of NALP3 in K562 cells with NALP3 antibody at 2 ug/mL.

• Reactivity: Hu, Mu, Ec
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, PAGE
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

NLRP3/NALP3 Antibody Summary

• Immunogen: NLRP3/NALP3 Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3. The immunogen is located within amino acids 120 - 170 of NALP3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 2 ug/mL
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • SDS-Page
• Application Notes: Use in SDS- Page and Immunoprecipitation reported in scientific literature (PMID:31551961).
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  NLRP3/NALP3 Protein (NBP1-77080PEP)

Reactivity Notes

E. coli reactivity reported in scientific literature (PMID: 31551961).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NLRP3/NALP3 Antibody

• AGTAVPRL
• AII
• AII/AVP
• Angiotensin/vasopressin receptor AII/AVP-like
• AVP
• C1orf7
• Caterpiller protein 1.1
• CIAS1
• CLR1.1
• Cold autoinflammatory syndrome 1 protein
• Cold autoinflammatory syndrome 1
• Cryopyrin
• Familial cold autoinflammatory syndrome
• FCAS
• FCU
• Leucine-rich repeat-, and PYD-containing protein 3
• Muckle-Wells syndrome
• MWS
• NACHT
• NACHT, LRR and PYD containing protein 3
• NACHT, LRR and PYD domains-containing protein 3
• NALP3
• NLR family, pyrin domain containing 3
• NLRP3
• Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
• PYPAF1
• PYRIN-containing APAF1-like protein 1

Background

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1betaand IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP3/NALP3 Antibody (NBP1-77080)(22)

Publications using NBP1-77080

• Verma V, Kumar P, Gupta S et al. Alpha-Hemolysin of uropathogenic E. coli regulates NLRP3 inflammasome activation and mitochondrial dysfunction in THP-1 macrophages Sci Rep Jul 28 2020 [PMID: 32724079] (IP, WB, Human)
• Toyohara T, Roudnicky F, Florido MHC et al. Patient hiPSCs Identify Vascular Smooth Muscle Arylacetamide Deacetylase as Protective against Atherosclerosis Cell Stem Cell May 14 2020 [PMID: 32413331] (IHC-P, Mouse)
• Kim H, Seo Js, Lee Sy et Al. AIM2 inflammasome contributes to brain injury and chronic post-stroke cognitive impairment in mice Brain Behav. Immun. Mar 19 2020 [PMID: 32201254] (WB, Mouse)
• Liang F, Huang T, Li B et al. High-intensity interval training and moderate-intensity continuous training alleviate beta-amyloid deposition by inhibiting NLRP3 inflammasome activation in APPswe/PS1dE9 mice Neuroreport Mar 25 2020 [PMID: 32150150]
• Verma V, Gupta S, Kumar P, et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections. Front Microbiol. 2019 Sep 03 [PMID: 31551961] (PAGE, WB, IP, E. coli)
• Liu JX, Yang C, Zhang WH et al. Disturbance of mitochondrial dynamics and mitophagy in sepsis-induced acute kidney injury Life Sci. Aug 31 2019 [PMID: 31479679] (IHC-P, Mouse)
• Geyfman M, Debabov D, Poloso N, Alvandi N. Mechanistic Insight into the Activity of a Sulfone Compound Dapsone on Propionibacterium (Newly Reclassified as Cutibacterium) Acnes-Mediated Cytokine Production Exp. Dermatol. Dec 26 2018 [PMID: 30585659] (WB, Human)
• Pan Z, Shan Q, Gu P, Wang XM. miRNA-23a/CXCR4 regulates neuropathic pain via directly targeting TXNIP/NLRP3 inflammasome axis. J Neuroinflammation. 2018 Jan 31 [PMID: 29386025] (WB, Mouse)
• Zhuang J, Wen X, Zhang YQ et al. TDP-43 upregulation mediated by the NLRP3 inflammasome induces cognitive impairment in 2 2',4,4'-tetrabromodiphenyl ether (BDE-47)-treated mice. Brain Behav. Immun. May 19 2017 [PMID: 28532818]
• Hua X, Yuan X et al. Desiccating stress worsens alkali burn injury by magnifying caspase-8-induced imbalance of NLRP3 and NLRP6. J Allergy Clin Immunol Jan 10 2017 [PMID: 28479336] (IHC, Mouse)
• Lee HI, Lee SW, Kim NG et al. Low-level light emitting diode (LED) therapy suppresses inflammasome-mediated brain damage in experimental ischemic stroke. J Biophotonics 2017 Feb 06 [PMID: 28164443]
• MacPherson Maximilian, Westbom Catherine, Kogan Helen, Shukla Arti Actin polymerization plays a significant role in asbestos-induced inflammasome activation in mesothelial cells in vitro. Histochem Cell Biol. 2016 Dec 24 [PMID: 28013367] (ICC/IF, Human)
• Zhu W, Cao FS, Feng J et al. NLRP3 inflammasome activation contributes to long-term behavioral alterations in mice injected with lipopolysaccharide. Neuroscience. Dec 5 2016 [PMID: 27923741] (WB, Mouse)
• Lu C, Xu W, Shao J et al. Nrf2 Activation Is Required for Ligustrazine to Inhibit Hepatic Steatosis in Alcohol-Preferring Mice and Hepatocytes Toxicol. Sci Feb 1 2017 [PMID: 27837167] (Mouse)
• Westbom Catherine, Thompson Joyce K, Leggett Alan et al. Inflammasome Modulation by Chemotherapeutics in Malignant Mesothelioma. PloS One 2015 [PMID: 26689911] (WB, Mouse)
• Chi W, Chen H, Li F et al. HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-kB pathway in acute glaucoma. J Neuroinflammation. 2015 Jul 30 [PMID: 26224068] (WB, Mouse)
• Fann DY, Santro T, Manzanero S et al. Intermittent fasting attenuates inflammasome activity in ischemic stroke. Exp. Neurol. 2014 May 05 [PMID: 24805069] (WB, Mouse)
• Yang-Wei Fann D, Lee SY et al. Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke. Cell Death Dis. 2013 Sep 5 [PMID: 24008734] (WB, Mouse)
• Kanneganti TD, Ozoren N, Body-Malapal M et al. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp. Nature; 440:233-6. 2006 [PMID: 16407888]
• Agostini L, Martinon F, Burns K et al. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity; 20:319-25. 2004 [PMID: 15030775]
• O'Connor W Jr, Hartin JA, Zhu X et al. Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties. J. Immunol. 171:6329-33. 2003 [PMID: 14662828]
• Chamaillard M, Girardin SE, Viala J et al. Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation. Cell. Microbiol. 5:581-92. 2003 [PMID: 12925128]

Reviews for NLRP3/NALP3 Antibody (NBP1-77080) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Max MacPherson IHC-P Mouse 11/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse lung
• Species: Mouse
• Lot: A-2

reviewed by: Anonymous IF Mouse 04/03/2015

Summary

• Application: Immunofluorescence
• Sample Tested: corneal epithelium in mouse
• Species: Mouse

Blocking

• Blocking Details: Blocking kit, apply for one hour, in room temperature

Details

• Detection Notes: 20 seconds for exposure

FAQs for NLRP3/NALP3 Antibody (NBP1-77080). (Showing 1 - 4 of 4 FAQs).

1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
   • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
2. Does NLRP3/NALP3 antibodies come in lyophilized form?
   • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
   • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
   • A sequence of 16 amino acides within the N-terminal of human NLRP3/NALP3.

Bioinformatics

• Gene Symbol: NLRP3
• Entrez:
  • 114548(Human)
  • 216799(Mouse)
• Uniprot:
  • Q96P20(Human)