NLRP3/NALP3 Antibody - BSA Free

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Orthogonal Strategies: Western Blot: NLRP3/NALP3 Antibody [NBP1-77080] - HMGB1 promoted the activation of canonical NLRP3 inflammasome in retinal IR injury. The intravitreal injection of HMGB1 inhibitor, GA, ...read more
Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody [NBP1-77080] - Staining of NALP3 in K562 cells with NALP3 antibody at 2 ug/mL.
Western Blot: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.

Product Details

Summary
Reactivity Hu, Mu, EcSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, PAGE
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
       

Orthogonal Strategies

 

Order Details

NLRP3/NALP3 Antibody - BSA Free Summary

Immunogen
NLRP3/NALP3 Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3. The immunogen is located within amino acids 120 - 170 of NALP3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NLRP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • SDS-Page
  • Western Blot 1 - 2 ug/mL
Application Notes
Use in SDS- Page and Immunoprecipitation reported in scientific literature (PMID:31551961).
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
NLRP3/NALP3 Protein (NBP1-77080PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-77080 in the following applications:

Publications
Read Publications using
NBP1-77080 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • 2 publications
  • IP
    2 publications
  • 1 publication
  • WB
    12 publications

Reactivity Notes

E. coli reactivity reported in scientific literature (PMID: 31551961).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NLRP3/NALP3 Antibody - BSA Free

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1betaand IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NLRP3/NALP3 Antibody (NBP1-77080)(25)

We have publications tested in 3 confirmed species: Human, Mouse, E. coli.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-P, IP, PAGE, WB.


Filter By Application
ICC/IF
(1)
IHC
(2)
IHC-P
(2)
IP
(2)
PAGE
(1)
WB
(12)
All Applications
Filter By Species
Human
(3)
Mouse
(13)
E. coli
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NBP1-77080 Applications Species
Jin L, Jin F, Guo S et al. Metformin Inhibits NLR Family Pyrin Domain Containing 3 (NLRP)-Relevant Neuroinflammation via an Adenosine-5-Monophosphate-Activated Protein Kinase (AMPK)-Dependent Pathway to Alleviate Early Brain Injury After Subarachnoid Hemorrhage in Mice Frontiers in Pharmacology Mar 17 2022 [PMID: 35370693] (IHC, Mouse) IHC Mouse
Gaul S, Schaeffer Km, Opitz L Et Al. Extracellular NLRP3 inflammasome particles are internalized by human coronary artery smooth muscle cells and induce pro-atherogenic effects Scientific reports Jul 26 2021 [PMID: 34312415] (WB) WB
Yu, C, Chen, P Et al. hADSCs derived extracellular vesicles inhibit NLRP3inflammasome activation and dry eye. Sci Rep Sep 3 2020 [PMID: 32884023] (WB, Mouse) WB Mouse
Verma V, Kumar P, Gupta S et al. Alpha-Hemolysin of uropathogenic E. coli regulates NLRP3 inflammasome activation and mitochondrial dysfunction in THP-1 macrophages Sci Rep Jul 28 2020 [PMID: 32724079] (IP, WB, Human) IP, WB Human
Toyohara T, Roudnicky F, Florido MHC et al. Patient hiPSCs Identify Vascular Smooth Muscle Arylacetamide Deacetylase as Protective against Atherosclerosis Cell Stem Cell May 14 2020 [PMID: 32413331] (IHC-P, Mouse) IHC-P Mouse
Kim H, Seo Js, Lee Sy et Al. AIM2 inflammasome contributes to brain injury and chronic post-stroke cognitive impairment in mice Brain Behav. Immun. Mar 19 2020 [PMID: 32201254] (WB, Mouse) WB Mouse
Liang F, Huang T, Li B et al. High-intensity interval training and moderate-intensity continuous training alleviate beta-amyloid deposition by inhibiting NLRP3 inflammasome activation in APPswe/PS1dE9 mice Neuroreport Mar 25 2020 [PMID: 32150150]
Verma V, Gupta S, Kumar P, et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections. Front Microbiol. 2019 Sep 03 [PMID: 31551961] (PAGE, WB, IP, E. coli) PAGE, WB, IP E. coli
Liu JX, Yang C, Zhang WH et al. Disturbance of mitochondrial dynamics and mitophagy in sepsis-induced acute kidney injury Life Sci. Aug 31 2019 [PMID: 31479679] (IHC-P, Mouse) IHC-P Mouse
Geyfman M, Debabov D, Poloso N, Alvandi N. Mechanistic Insight into the Activity of a Sulfone Compound Dapsone on Propionibacterium (Newly Reclassified as Cutibacterium) Acnes-Mediated Cytokine Production Exp. Dermatol. Dec 26 2018 [PMID: 30585659] (WB, Human) WB Human
Show All 25 Publications.

Reviews for NLRP3/NALP3 Antibody (NBP1-77080) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-77080:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
Immunofluorescence
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin NLRP3/NALP3 NBP1-77080
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4
reviewed by:
Verified Customer
Immunohistochemistry-Paraffin Mouse 11/05/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse lung
SpeciesMouse
LotA-2
  4
reviewed by:
De-quan Li
Immunofluorescence Mouse 04/03/2015
View

Summary

ApplicationImmunofluorescence
Sample Testedcorneal epithelium in mouse
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NLRP3/NALP3 Antibody (NBP1-77080). (Showing 1 - 4 of 4 FAQs).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
    • A sequence of 16 amino acides within the N-terminal of human NLRP3/NALP3.

Secondary Antibodies

 

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Bioinformatics Tool for NLRP3/NALP3 Antibody (NBP1-77080)

Discover related pathways, diseases and genes to NLRP3/NALP3 Antibody (NBP1-77080). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for NLRP3/NALP3 Antibody (NBP1-77080)

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Blogs on NLRP3/NALP3.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
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Verified Customer
11/05/2015
Application: Immunohistochemistry-Paraffin
Species: Mouse

 
De-quan Li
04/03/2015
Application: Immunofluorescence
Species: Mouse

Bioinformatics

Gene Symbol NLRP3
Entrez
Uniprot