NLRP3/NALP3 Antibody

Images

 
Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.
Western Blot: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody [NBP1-77080] - Staining of NALP3 in K562 cells with NALP3 antibody at 2 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, EcSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP, PAGE
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

NLRP3/NALP3 Antibody Summary

Immunogen
NLRP3/NALP3 Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3. The immunogen is located within amino acids 120 - 170 of NALP3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NLRP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • SDS-Page
Application Notes
Use in SDS- Page and Immunoprecipitation reported in scientific literature (PMID:31551961).
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
NLRP3/NALP3 Protein (NBP1-77080PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-77080 in the following applications:

Publications
Read Publications using
NBP1-77080 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 2 publications
  • IP
    2 publications
  • 1 publication
  • WB
    10 publications

Reactivity Notes

E. coli reactivity reported in scientific literature (PMID: 31551961).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NLRP3/NALP3 Antibody

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1betaand IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NLRP3/NALP3 Antibody (NBP1-77080)(22)

We have publications tested in 3 confirmed species: Human, Mouse, E. coli.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-P, IP, PAGE, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(2)
IP
(2)
PAGE
(1)
WB
(10)
All Applications
Filter By Species
Human
(3)
Mouse
(11)
E. coli
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NBP1-77080 Applications Species
Verma V, Kumar P, Gupta S et al. Alpha-Hemolysin of uropathogenic E. coli regulates NLRP3 inflammasome activation and mitochondrial dysfunction in THP-1 macrophages Sci Rep Jul 28 2020 [PMID: 32724079] (IP, WB, Human) IP, WB Human
Toyohara T, Roudnicky F, Florido MHC et al. Patient hiPSCs Identify Vascular Smooth Muscle Arylacetamide Deacetylase as Protective against Atherosclerosis Cell Stem Cell May 14 2020 [PMID: 32413331] (IHC-P, Mouse) IHC-P Mouse
Kim H, Seo Js, Lee Sy et Al. AIM2 inflammasome contributes to brain injury and chronic post-stroke cognitive impairment in mice Brain Behav. Immun. Mar 19 2020 [PMID: 32201254] (WB, Mouse) WB Mouse
Liang F, Huang T, Li B et al. High-intensity interval training and moderate-intensity continuous training alleviate beta-amyloid deposition by inhibiting NLRP3 inflammasome activation in APPswe/PS1dE9 mice Neuroreport Mar 25 2020 [PMID: 32150150]
Verma V, Gupta S, Kumar P, et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections. Front Microbiol. 2019 Sep 03 [PMID: 31551961] (PAGE, WB, IP, E. coli) PAGE, WB, IP E. coli
Liu JX, Yang C, Zhang WH et al. Disturbance of mitochondrial dynamics and mitophagy in sepsis-induced acute kidney injury Life Sci. Aug 31 2019 [PMID: 31479679] (IHC-P, Mouse) IHC-P Mouse
Geyfman M, Debabov D, Poloso N, Alvandi N. Mechanistic Insight into the Activity of a Sulfone Compound Dapsone on Propionibacterium (Newly Reclassified as Cutibacterium) Acnes-Mediated Cytokine Production Exp. Dermatol. Dec 26 2018 [PMID: 30585659] (WB, Human) WB Human
Pan Z, Shan Q, Gu P, Wang XM. miRNA-23a/CXCR4 regulates neuropathic pain via directly targeting TXNIP/NLRP3 inflammasome axis. J Neuroinflammation. 2018 Jan 31 [PMID: 29386025] (WB, Mouse) WB Mouse
Zhuang J, Wen X, Zhang YQ et al. TDP-43 upregulation mediated by the NLRP3 inflammasome induces cognitive impairment in 2 2',4,4'-tetrabromodiphenyl ether (BDE-47)-treated mice. Brain Behav. Immun. May 19 2017 [PMID: 28532818]
Hua X, Yuan X et al. Desiccating stress worsens alkali burn injury by magnifying caspase-8-induced imbalance of NLRP3 and NLRP6. J Allergy Clin Immunol Jan 10 2017 [PMID: 28479336] (IHC, Mouse) IHC Mouse
Show All 22 Publications.

Reviews for NLRP3/NALP3 Antibody (NBP1-77080) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-77080:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin NLRP3/NALP3 NBP1-77080
Enlarge
4
reviewed by:
Max MacPherson
IHC-P Mouse 11/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse lung
SpeciesMouse
LotA-2
  4
reviewed by:
Anonymous
IF Mouse 04/03/2015
View

Summary

ApplicationImmunofluorescence
Sample Testedcorneal epithelium in mouse
SpeciesMouse

Blocking

Blocking DetailsBlocking kit, apply for one hour, in room temperature

Details

Detection Notes20 seconds for exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NLRP3/NALP3 Antibody (NBP1-77080). (Showing 1 - 4 of 4 FAQs).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
    • A sequence of 16 amino acides within the N-terminal of human NLRP3/NALP3.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77080AF350
Alexa Fluor 405 NBP1-77080AF405
Alexa Fluor 488 NBP1-77080AF488
Alexa Fluor 532 NBP1-77080AF532
Alexa Fluor 594 NBP1-77080AF594
Alexa Fluor 647 NBP1-77080AF647
Alexa Fluor 700 NBP1-77080AF700
Alexa Fluor 750 NBP1-77080AF750
Biotin NBP1-77080B
DyLight 350 NBP1-77080UV
DyLight 405 NBP1-77080V
DyLight 488 NBP1-77080G
DyLight 550 NBP1-77080R
DyLight 594 NBP1-77080DL594
DyLight 650 NBP1-77080C
DyLight 680 NBP1-77080FR
DyLight 755 NBP1-77080IR
FITC NBP1-77080F
HRP NBP1-77080H
Janelia Fluor 549 NBP1-77080JF549
Janelia Fluor 646 NBP1-77080JF646

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Blogs on NLRP3/NALP3.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Max MacPherson
11/05/2015
Application: IHC-P
Species: Mouse

 
Anonymous
04/03/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NLRP3
Entrez
Uniprot