NLRP3/NALP3 Antibody

Images

 
Western Blot: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.
Immunocytochemistry: NLRP3/NALP3 Antibody [NBP1-77080] - Staining of NALP3 in K562 cells with NALP3 antibody at 2 ug/mL.
Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody [NBP1-77080] - K562 cells with NALP3 antibody at 20 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P, ICC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

NLRP3/NALP3 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3. The immunogen is located within amino acids 120 - 170 of NALP3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NLRP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry-Paraffin
  • Immunocytochemistry
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
NLRP3/NALP3 Protein (NBP1-77080PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-77080 in the following applications:

Publications
Read Publications using
NBP1-77080 in the following applications:

  • WB
    4 publications

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine: (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NLRP3/NALP3 Antibody

  • AGTAVPRL
  • AII
  • AII/AVP
  • Angiotensin/vasopressin receptor AII/AVP-like
  • AVP
  • C1orf7
  • Caterpiller protein 1.1
  • CIAS1
  • CLR1.1
  • Cold autoinflammatory syndrome 1 protein
  • Cold autoinflammatory syndrome 1
  • Cryopyrin
  • Familial cold autoinflammatory syndrome
  • FCAS
  • FCU
  • Leucine-rich repeat-, and PYD-containing protein 3
  • Muckle-Wells syndrome
  • MWS
  • NACHT
  • NACHT, LRR and PYD containing protein 3
  • NACHT, LRR and PYD domains-containing protein 3
  • NALP3
  • NLR family, pyrin domain containing 3
  • NLRP3
  • Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
  • PYPAF1
  • PYRIN-containing APAF1-like protein 1

Background

NALP3, a member of Nod-like receptors, has a crucial role in inflammation and immunity and may be a proximal sensor of cellular stress and danger signals. NALP3 forms a caspase-1 activating molecular complex termed the inflammasome. The inflammasome allows activation of IL-1beta, the key player of the inflammation and fever. NALP3 gene encodes a pyrin like protein which contains a pyrin domain, a nucleotide binding site (NBS) domain, and a leucine rich repeat (LRR) motif. NALP3 protein interacts with apoptosis associated speck like protein containing a CARD and may function as an inducer of apoptosis and an activator of NF-kappaB signaling. Defects in NALP3 have been associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NLRP3/NALP3 Antibody (NBP1-77080)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
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Filter By Species
Mouse
(5)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-77080 Applications Species
Zhuang J, Wen X, Zhang YQ et al. TDP-43 upregulation mediated by the NLRP3 inflammasome induces cognitive impairment in 2 2',4,4'-tetrabromodiphenyl ether (BDE-47)-treated mice. Brain Behav. Immun. May 19 2017 [PMID: 28532818]
Lee HI, Lee SW, Kim NG et al. Low-level light emitting diode (LED) therapy suppresses inflammasome-mediated brain damage in experimental ischemic stroke. J Biophotonics 2017 Feb 06 [PMID: 28164443]
Zhu W, Cao FS, Feng J et al. NLRP3 inflammasome activation contributes to long-term behavioral alterations in mice injected with lipopolysaccharide. Neuroscience. Dec 5 2016 [PMID: 27923741] (WB, Mouse) WB Mouse
Lu C, Xu W, Shao J et al. Nrf2 Activation Is Required for Ligustrazine to Inhibit Hepatic Steatosis in Alcohol-Preferring Mice and Hepatocytes Toxicol. Sci Feb 1 2017 [PMID: 27837167] (Mouse) Mouse
Chi W, Chen H, Li F et al. HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-kB pathway in acute glaucoma. J Neuroinflammation. 2015 Jul 30 [PMID: 26224068] (WB, Mouse) WB Mouse
Fann DY, Santro T, Manzanero S et al. Intermittent fasting attenuates inflammasome activity in ischemic stroke. Exp. Neurol. 2014 May 05 [PMID: 24805069] (WB, Mouse) WB Mouse
Yang-Wei Fann D, Lee SY et al. Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke. Cell Death Dis. 2013 Sep 5 [PMID: 24008734] (WB, Mouse) WB Mouse
Kanneganti TD, Ozoren N, Body-Malapal M et al. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp. Nature; 440:233-6. 2006 [PMID: 16407888]
Agostini L, Martinon F, Burns K et al. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity; 20:319-25. 2004 [PMID: 15030775]
O'Connor W Jr, Hartin JA, Zhu X et al. Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties. J. Immunol. 171:6329-33. 2003 [PMID: 14662828]
Show All 11 Publications.

Reviews for NLRP3/NALP3 Antibody (NBP1-77080) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 application: IHC-P, IF.

Reviews using NBP1-77080:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin NLRP3/NALP3 NBP1-77080
Enlarge
reviewed by:
Max MacPherson
IHC-P Mouse 11/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse lung
SpeciesMouse
LotA-2
 
reviewed by:
Anonymous
IF Mouse 04/03/2015
View

Summary

ApplicationImmunofluorescence
Sample Testedcorneal epithelium in mouse
SpeciesMouse

Blocking

Blocking DetailsBlocking kit, apply for one hour, in room temperature

Details

Detection Notes20 seconds for exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NLRP3/NALP3 Antibody (NBP1-77080). (Showing 1 - 4 of 4 FAQs).

  1. Are the NLRP3/NALP3 antibodies validated in Simple Western?
    • Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.
  2. Does NLRP3/NALP3 antibodies come in lyophilized form?
    • Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.
  3. What is the theoretical molecular weight for NLRP3/NALP3 antibodies?
    • The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.
  4. What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?
    • A sequence of 16 amino acides within the N-terminal of human NLRP3/NALP3.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for NLRP3/NALP3 Antibody (NBP1-77080)

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Blogs on NLRP3/NALP3.

NLRP3/NALP3 - Sensing and responding to pathogen infection
The inflammasome is a multi protein complex that is an important component of the innate immune response. The inflammasome is able to sense and respond to pathogen infections by recognizing pathogen-associated molecular patterns and mediating the ...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Max MacPherson
11/05/2015
Application: IHC-P
Species: Mouse

 
Anonymous
04/03/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NLRP3
Entrez
Uniprot