preproANP Antibody

Images

 
Western Blot: preproANP Antibody [NBP2-14873] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Mouse muscle. ANP stained by ANP antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: preproANP Antibody [NBP2-14873] - Knockdown of ATE1 attenuates cardiac hypertrophy. Western blot analysis of preproANP protein levels in heart samples from sham and renal ligated rats (B2) bar graph was ...read more
Western Blot: preproANP Antibody [NBP2-14873] - Knockdown of ATE1 attenuates cardiac hypertrophy. Western blot analysis of preproANP protein levels in heart samples from sham and renal ligated rats (B2) bar graph was ...read more
Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Human hepatoma, using ANP antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Mouse muscle. ANP antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Mouse heart. ANP stained by preproANP antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Mouse heart. preproANP stained by preproANP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Rat heart. ANP stained by ANP antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Knockdown of ATE1 attenuates cardiac hypertrophy. Decreased mRNA levels in (B) ANP (B1) Western blot analysis of ANP protein levels in heart samples from sham and renal ligated rats (B2). Image collected and cropped by ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

preproANP Antibody Summary

Immunogen
Full length human preproANP Recombinant protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPPA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-14873 in the following applications:

  • WB
    5 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (83%), Porcine (87%), Rabbit (81%), Bovine (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for preproANP Antibody

  • ANF
  • ANP
  • ATFB6
  • atrial natriuretic factor prohormone
  • atrial natriuretic peptide prohormone
  • ATRST2
  • CDD
  • CDD-ANF
  • CDP
  • natriuretic peptides A
  • PND

Background

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for preproANP Antibody (NBP2-14873)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: WB.


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(5)
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(4)
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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol NPPA
Uniprot