Myeloperoxidase/MPO Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Myeloperoxidase/MPO Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Myeloperoxidase/MPO
  Met16-Thr718
  Accession # AAR99349
• Specificity: Detects mouse Myeloperoxidase/MPO in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human MPO is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MPO
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myeloperoxidase/MPO Antibody [Biotin]

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide-dependent conversion of chloride, bromide, and iodide to multiple reactive species (1). Post-translational processing of human MPO involves the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide (2). This results in a disulfide-linked dimer composed of a 60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond between the heavy chains (2). Mouse and human MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and the formation of 3‑chlorotyrosine and dityrosine crosslinks (4‑6). Modification of ApoB100, as well as the lipid and cholesterol components of LDL and HDL, promotes the development of atherosclerosis (5, 7‑9). MPO is also associated with a variety of other diseases (1), and inhibits vasodilation in inflammation by depleting the levels of NO (10). Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells (11). MPO is stored in neutrophil azurophilic granules. Upon cellular activation, it is deposited into pathogen‑containing phagosomes (2). While mice lacking MPO are impaired in clearing select microbial infections, MPO deficiency in humans does not necessarily result in heightened susceptibility to infections (12, 13).

1. Klebanoff, S.J. (2005) J. Leukoc. Biol. 77:598.
2. Hansson, M. et al. (2006) Arch. Biochem. Biophys. 445:214.
3. Hashinaka, K. et al. (1988) Biochemistry 27:5906.
4. van Dalen, C.J. et al. (2000) J. Biol. Chem. 275:11638.
5. Hazen, S.L. and J.W. Heinecke (1997) J. Clin. Invest. 99:2075.
6. Heinecke, J.W. et al. (1993) J. Clin. Invest. 91:2866.
7. Podrez, E.A. et al. (1999) J. Clin. Invest. 103:1547.
8. Bergt, C. et al. (2004) Proc. Natl. Acad. Sci. 101:13032.
9. Hazen, S.L. et al. (1996) J. Biol. Chem. 271:23080.
10. Eiserich, J.P. et al. (2002) Science 296:2391.
11. Tiruppathi, C. et al. (2004) Proc. Natl. Acad. Sci. 101:7699.
12. Aratani Y. et al. (2000) J. Infect. Dis. 182:1276.
13. Kutter, D. (1998) J. Mol. Med. 76:669.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloperoxidase/MPO Antibody (BAF3667)(4)

Publications using BAF3667

• Vicanolo, T;Özcan, A;Li, JL;Huerta-López, C;Ballesteros, I;Rubio-Ponce, A;Dumitru, AC;Nicolás-Ávila, JÁ;Molina-Moreno, M;Reyes-Gutierrez, P;Johnston, AD;Martone, C;Greto, E;Quílez-Alvarez, A;Calvo, E;Bonzon-Kulichenko, E;Álvarez-Velez, R;Chooi, MY;Kwok, I;González-Bermúdez, B;Malleret, B;Espinosa, FM;Zhang, M;Wang, YL;Sun, D;Zhen Chong, S;El-Armouche, A;Kim, KK;Udalova, IA;Greco, V;Garcia, R;Vázquez, J;Dopazo, A;Plaza, GR;Alegre-Cebollada, J;Uderhardt, S;Ng, LG;Hidalgo, A; Matrix-producing neutrophils populate and shield the skin Nature 2025-03-19 [PMID: 40108463] (Immunocytochemistry, Mouse)
• Leifer, VP;Fang, F;Song, L;Kim, J;Papanikolaou, JF;Smeeton, J;Thomopoulos, S; Single-cell RNA-sequencing analysis of immune and mesenchymal cell crosstalk in the developing enthesis Scientific reports 2024-11-05 [PMID: 39500962] (Immunohistochemistry, Mouse)
• Yalcinkaya M, Fotakis P, Liu W et al. Cholesterol accumulation in macrophages drives NETosis in atherosclerotic plaques via IL-1b secretion Cardiovascular research 2022-12-20 [PMID: 36537208]
• Wenli Liu, Nataliya Östberg, Mustafa Yalcinkaya, Huijuan Dou, Kaori Endo-Umeda, Yang Tang et al. Erythroid lineage Jak2V617F expression promotes atherosclerosis through erythrophagocytosis and macrophage ferroptosis Journal of Clinical Investigation 2022-07-01 [PMID: 35587375]

Bioinformatics

• Gene Symbol: MPO
• Uniprot:
  • AAR99349()