Proteinase 3/Myeloblastin/PRTN3 Antibody

Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.

Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.

Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess blocking solution was removed and cells were incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.

Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.

Immunocytochemistry/Immunofluorescence: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PBMC were isolated and adjusted to 106 cells. Cells were fixed with 2% formaldehyde for 10 min at 37C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 min at RT. Excess of blocking solution was removed and cells were then incubated with Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 min. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit Ab to c-terminal region of Pr3 (Wegener autoantigen): whole serum.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Proteinase 3/Myeloblastin/PRTN3 Antibody Summary

• Description: Novus Biologicals Rabbit Proteinase 3/Myeloblastin/PRTN3 Antibody (NBP1-25966) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-Proteinase 3/Myeloblastin/PRTN3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from the c-terminal region of human Proteinase 3/Myeloblastin/PRTN3 conjugated to blue carrier protein was used as the antigen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRTN3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:200-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:200-1:1000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for Proteinase 3/Myeloblastin/PRTN3 Antibody

• ACPA
• AGP7
• AGP7PR3
• azurophil granule protein 7
• C-ANCA
• EC 3.4.21
• EC 3.4.21.76
• Leukocyte proteinase 3
• MBN
• MBT
• Myeloblastin
• Neutrophil proteinase 4
• NP4
• NP-4
• P29C-ANCA antigen
• PR-3CANCA
• Proteinase 3
• PRTN3
• serine proteinase, neutrophil
• Wegener autoantigen
• Wegener granulomatosis autoantigen

Background

FUNCTION: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. Catalytic activity: Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proteinase 3/Myeloblastin/PRTN3 Antibody (NBP1-25966)(2)

Publications using NBP1-25966

• Kolonin Mikhail G, Sergeeva Anna, Staquicini Daniela I et al. Interaction between Tumor Cell Surface Receptor RAGE and Proteinase 3 Mediates Prostate Cancer Metastasis to Bone. Cancer Research 2017-04-20 [PMID: 28428279] (ICC/IF, Human)
• Hao J, Wang C, Yuan J et al. A Pro-Inflammatory Role of C5L2 in C5a-Primed Neutrophils for ANCA-Induced Activation. PLoS One 2013-06-13 [PMID: 23785491] (FLOW, Human)

Bioinformatics

• Gene Symbol: PRTN3
• Entrez:
  • 5657(Human)
• Uniprot:
  • P24158(Human)