Human C-Reactive Protein/CRP DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human C-Reactive Protein/CRP DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CRP

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human C-Reactive Protein/CRP DuoSet ELISA, 15 Plate

• C-Reactive Protein
• C-reactive protein, pentraxin-related
• CRP
• MGC88244
• pentraxin 1
• PTX1MGC149895

Background

C-Reactive Protein (CRP), also known as Pentraxin 1, is a non-glycosylated protein in the Pentraxin family that also includes Pentraxin 2/SAP and Pentraxin 3/TSG-14. CRP functions as a sensor and activator of the innate immune response (1). In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation (2). In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2 (3). CRP assembles non-covalently into a 110-120 kDa cyclical pentamer (4). Mature human CRP shares 71% and 64% amino acid (aa) sequence identity with mouse and rat CRP, respectively (5). 

CRP binds and opsonizes apoptotic cells (6-8) as well as bacteria such as S. pneumoniae (9, 10). It subsequently enhances the phagocytosis of these opsonized cells (6, 8-10). CRP additionally binds several proteins in the complement cascade including C1q, C4BP, and Factor H (8, 11-13). It enhances activation of the classical complement pathway and the deposition of C3b (9). In later stages of the response, CRP inhibits complement-mediated cell lysis through its binding to C4BP and Factor H (8, 12). These interactions induce the upregulation of complement inhibitory proteins CD46, CD59, and CD55/DAF and inhibit assembly of the membrane attack complex (MAC) (8, 14). 

CRP binds to Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells (15-17), and Fc receptors are required for the phagocytosis of CRP-opsonized target cells (6, 10, 18). CRP binding to Fc gamma RI induces Src activation which subsequently triggers the inhibitory Fc gamma RIIb and dampens the inflammatory response (15, 19). CRP additionally promotes dendritic cell maturation and humoral immunity (10). In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in coronary artery infarction, and inhibits the repair of injured vascular endothelium (7, 19, 20). 

Publications for C-Reactive Protein/CRP (DY1707)(54)

Publications using DY1707

• WJ Shon, MH Jung, Y Kim, GH Kang, EY Choi, DM Shin Sugar-sweetened beverages exacerbate high-fat diet-induced inflammatory bowel disease by altering the gut microbiome: Sweetened beverages enhance fat-induced IBD The Journal of nutritional biochemistry, 2022;0(0):109254. 2022 [PMID: 36572070] (Human)
• MJM Silvis, EJ Demkes, L Timmers, F Arslan, SCA de Jager, JPG Sluijter, A Mosterd, DPV de Kleijn, L Bosch, GPJ van Hout NLRP3-Inflammasome Inhibition with IZD334 Does Not Reduce Cardiac Damage in a Pig Model of Myocardial Infarction Biomedicines, 2022;10(12):. 2022 [PMID: 36551811] (Porcine)
• ML Sarangam, R Namazzi, D Datta, C Bond, CPB Vanderpool, RO Opoka, CC John, AL Conroy Intestinal Injury Biomarkers Predict Mortality in Pediatric Severe Malaria MBio, 2022;0(0):e0132522. 2022 [PMID: 36069443] (Human)
• J Mohanraj, UJA D'Souza, SY Fong, IR Karkada, H Jaiprakash Association between Leptin (G2548A) and Leptin Receptor (Q223R) Polymorphisms with Plasma Leptin, BMI, Stress, Sleep and Eating Patterns among the Multiethnic Young Malaysian Adult Population from a Healthcare University Oncogene, 2022;19(14):. 2022 [PMID: 35886710] (Human)
• HM Demerdash, AA Sabry, OE Arida Impact of Zinc to Copper Ratio and Lipocalin 2 in Obese Patients Undergoing Sleeve Gastrectomy Oncogene, 2022;2022(0):9278531. 2022 [PMID: 35722455] (Human)
• R Palika, T Dasi, S Ghosh, R Peter, DJ Parasannan, AS Pradhan, AV Kurpad, HS Sachdev, B Kulkarni, R Pullakhand Efficacy of iron-folic acid treatment for reducing anemia prevalence and improving iron status in women of reproductive age: A one-year longitudinal study Clinical Nutrition ESPEN, 2022;49(0):390-397. 2022 [PMID: 35623842] (Human)
• C Pham, S Bekkering, M O'Hely, D Burgner, S Thomson, P Vuillermin, F Collier, W Marx, T Mansell, C Symeonides, PD Sly, M Lk Tang, R Saffery, AL Ponsonby Infant inflammation predicts childhood emotional and behavioral problems and partially mediates socioeconomic disadvantage Brain, Behavior, and Immunity, 2022;0(0):. 2022 [PMID: 35618227] (Human)
• T Mansell, R Saffery, S Burugupall, AL Ponsonby, MLK Tang, M O'Hely, S Bekkering, AAT Smith, R Rowland, S Ranganatha, PD Sly, P Vuillermin, F Collier, P Meikle, D Burgner, Barwon Inf Early life infection and proinflammatory, atherogenic metabolomic and lipidomic profiles in infancy: a population-based cohort study Elife, 2022;11(0):. 2022 [PMID: 35535496] (Human)
• MH Schutte, R Kleemann, NM Nota, CM Wiepjes, JM Snabel, G T'Sjoen, A Thijs, M den Heijer The effect of transdermal gender-affirming hormone therapy on markers of inflammation and hemostasis PLoS ONE, 2022;17(3):e0261312. 2022 [PMID: 35290388] (Human)
• S Hakki, EJ Robinson, MG Robson Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula Scientific Reports, 2022;12(1):1427. 2022 [PMID: 35082332] (Human)
• SKL Hogendoorn, L Lhopitalli, M Richard-Gr, E Tenisch, Z Mbarack, J Samaka, T Mlaganile, A Mamin, B Genton, L Kaiser, V D'Acremont, KC Kain, N Boillat-Bl Clinical sign and biomarker-based algorithm to identify bacterial pneumonia among outpatients with lower respiratory tract infection in Tanzania BMC infectious diseases, 2022;22(1):39. 2022 [PMID: 34991507] (Human)
• W Marx, S Thomson, M O'Hely, C Symeonides, F Collier, MLK Tang, A Loughman, D Burgner, R Saffery, C Pham, T Mansell, PD Sly, P Vuillermin, S Ranganatha, AL Ponsonby, Barwon Inf Maternal inflammatory and omega-3 fatty acid pathways mediate the association between socioeconomic disadvantage and childhood cognition Brain, Behavior, and Immunity, 2021;100(0):211-218. 2021 [PMID: 34896180] (Human)
• J Hesson, N Fudge, M Grant Cytomegalovirus Immunity, Inflammation and Cognitive Abilities in the Elderly Viruses, 2021;13(11):. 2021 [PMID: 34835127] (Human)
• GH Brody, T Yu, E Chen, M Kobor, SRH Beach, MK Lei, A Barr, DT Lin, GE Miller Risky family climates presage increased cellular aging in young adulthood Psychoneuroendocrinology, 2021;130(0):105256. 2021 [PMID: 34058561] (Human)
• R Fernandez-, MW Plankey, D Ware, J Bordon Changes in liver steatosis in HIV-positive women are associated with the BMI, but not with biomarkers Cytokine, 2021;0(0):155573. 2021 [PMID: 33994069] (Human)
• MJ de Smit, J Westra, MD Posthumus, G Springer, AJ van Winkel, A Vissink, E Brouwer, M Bijl Effect of Anti-Rheumatic Treatment on the Periodontal Condition of Rheumatoid Arthritis Patients International Journal of Environmental Research and Public Health, 2021;18(5):. 2021 [PMID: 33806304] (Human)
• MT Aung, Y Yu, KK Ferguson, DE Cantonwine, L Zeng, TF McElrath, S Pennathur, B Mukherjee, JD Meeker Cross-Sectional Estimation of Endogenous Biomarker Associations with Prenatal Phenols, Phthalates, Metals, and Polycyclic Aromatic Hydrocarbons in Single-Pollutant and Mixtures Analysis Approaches Environmental health perspectives, 2021;129(3):37007. 2021 [PMID: 33761273] (Human)
• J Shi, W Zhang, Y Niu, N Lin, X Li, H Zhang, R Hu, G Ning, J Fan, L Qin, Q Su, Z Yang Association of circulating proprotein convertase subtilisin/kexin type 9 levels and the risk of incident type 2 diabetes in subjects with prediabetes: a population-based cohort study Cardiovascular Diabetology, 2020;19(1):209. 2020 [PMID: 33302966] (Human)
• MT Aung, Y Song, KK Ferguson, DE Cantonwine, L Zeng, TF McElrath, S Pennathur, JD Meeker, B Mukherjee Application of an analytical framework for multivariate mediation analysis of environmental data Nat Commun, 2020;11(1):5624. 2020 [PMID: 33159049] (Human)
• DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects Eur J Immunol, 2020;0(0):. 2020 [PMID: 33125159] (Human)
• X Wang, J Shi, B Lu, W Zhang, Y Yang, J Wen, R Hu, Z Yang, X Wang Circulating heat shock protein 27 as a novel marker of subclinical atherosclerosis in type 2 diabetes: a cross-sectional community-based study BMC Cardiovasc Disord, 2020;20(1):198. 2020 [PMID: 32334520] (Human)
• S Sindhu, S Kochumon, R Thomas, A Bennakhi, F Al-Mulla, R Ahmad Enhanced Adipose Expression of Interferon Regulatory Factor (IRF)-5 Associates with the Signatures of Metabolic Inflammation in Diabetic Obese Patients Cells, 2020;9(3):. 2020 [PMID: 32188105] (Human)
• TN Chimponda, C Mushayi, DNM Osakunor, A Vengesai, E Enwono, S Amanfo, J Murray, C Tshuma, F Mutapi, T Mduluza Elevation of C-reactive protein, P-selectin and Resistin as potential inflammatory biomarkers of urogenital Schistosomiasis exposure in preschool children BMC Infect. Dis., 2019;19(1):1071. 2019 [PMID: 31856765] (Human)
• MT Aung, Y Yu, KK Ferguson, DE Cantonwine, L Zeng, TF McElrath, S Pennathur, B Mukherjee, JD Meeker Prediction and associations of preterm birth and its subtypes with eicosanoid enzymatic pathways and inflammatory markers Sci Rep, 2019;9(1):17049. 2019 [PMID: 31745121] (Human)
• S Sindhu, R Thomas, S Kochumon, A Wilson, M Abu-Farha, A Bennakhi, F Al-Mulla, R Ahmad Increased Adipose Tissue Expression of Interferon Regulatory Factor (IRF)-5 in Obesity: Association with Metabolic Inflammation Cells, 2019;8(11):. 2019 [PMID: 31718015] (Human)
• DY Arce, A Bellavia, DE Cantonwine, OJ Napoli, JD Meeker, T James-Todd, TF McElrath, LC Tsen Average and time-specific maternal prenatal inflammatory biomarkers and the risk of labor epidural associated fever PLoS ONE, 2019;14(11):e0222958. 2019 [PMID: 31689293] (Human)
• TT Tsai, TH Huang, NY Ho, YP Chen, CA Chen, CF Chen Development of a multiplex and sensitive lateral flow immunoassay for the diagnosis of periprosthetic joint infection Sci Rep, 2019;9(1):15679. 2019 [PMID: 31666656] (Human)
• RE Elphinston, AM Weckman, CR McDonald, V Tran, K Zhong, M Madanitsa, L Kalilani-P, C Khairallah, SM Taylor, SR Meshnick, V Mwapasa, FO Ter Kuile, AL Conroy, KC Kain Early malaria infection, dysregulation of angiogenesis, metabolism and inflammation across pregnancy, and risk of preterm birth in Malawi: A cohort study PLoS Med., 2019;16(10):e1002914. 2019 [PMID: 31574087] (Human)
• CR McDonald, AM Weckman, AL Conroy, P Olwoch, P Natureeba, MR Kamya, DV Havlir, G Dorsey, KC Kain Systemic inflammation is associated with malaria and preterm birth in women living with HIV on antiretrovirals and co-trimoxazole Sci Rep, 2019;9(1):6758. 2019 [PMID: 31043691] (Human)
• RK Cardoso, AM Araujo, RB Orcy, M Bohlke, JP Oses, FB Del Vecchi, FC Barcellos, MC Gonzalez, AJ Rombaldi Effects of continuous moderate exercise with partial blood flow restriction during hemodialysis: A protocol for a randomized clinical trial MethodsX, 2019;6(0):190-198. 2019 [PMID: 30740314] (Human)
• KK Venkatesh, JD Meeker, DE Cantonwine, TF McElrath, KK Ferguson Association of antenatal depression with oxidative stress and impact on spontaneous preterm birth J Perinatol, 2019;0(0):. 2019 [PMID: 30723278] (Human)
• Ellingsen T, Lappegard J, Ueland T, Aukrust P, Braekkan S, Hansen J Plasma hepcidin is associated with future risk of venous thromboembolism. Blood Adv, 0;2(11):1191-1197. 0 [PMID: 29844204] (Human)
• C Lahoz, E Castillo, JM Mostaza, O de Dios, MA Salinero-F, T González-A, F García-Igl, E Estirado, F Laguna, V Sanchez, C Sabín, S López, V Cornejo, C de Burgos, C Garcés, Relationship of the Adherence to a Mediterranean Diet and Its Main Components with CRP Levels in the Spanish Population Nutrients, 2018;10(3):. 2018 [PMID: 29558396] (Human)
• F Carbone, E Nulli Migl, A Bonaventur, A Vecchié, S De Vuono, MA Ricci, G Vaudo, M Boni, F Dallegri, F Montecucco, G Lupattelli High serum levels of C-reactive protein (CRP) predict beneficial decrease of visceral fat in obese females after sleeve gastrectomy Nutr Metab Cardiovasc Dis, 2018;0(0):. 2018 [PMID: 29502925] (Human)
• AR Morgan, C O'Hagan, S Touchard, S Lovestone, B Paul Morga Effects of freezer storage time on levels of complement biomarkers BMC Res Notes, 2017;10(1):559. 2017 [PMID: 29110694] (Human)
• T Ueland, LE Laugsand, LJ Vatten, I Janszky, C Platou, AE Michelsen, JK Damås, P Aukrust, BO Åsvold Monocyte/macrophage and T cell activation markers are not independently associated with MI risk in healthy individuals - results from the HUNT Study Int. J. Cardiol., 2017;0(0):. 2017 [PMID: 28615143] (Human)
• W Zhang, Z Yang, Y Niu, X Li, L Zhu, S Lu, H Zhang, J Fan, G Ning, L Qin, Q Su Association of calf circumference with insulin resistance and non-alcohol fatty liver disease: the REACTION study BMC Endocr Disord, 2017;17(1):28. 2017 [PMID: 28558676] (Human)
• A Burban, A Sharanek, R Hüe, M Gay, S Routier, A Guillouzo, C Guguen-Gui Penicillinase-resistant antibiotics induce non-immune-mediated cholestasis through HSP27 activation associated with PKC/P38 and PI3K/AKT signaling pathways Sci Rep, 2017;7(1):1815. 2017 [PMID: 28500348] (Human)
• CA Castro, KA da Silva, MM Buffo, KNZ Pinto, FO Duarte, KO Nonaka, FF Aníbal, ACGO Duarte Experimental type 2 diabetes induction reduces serum vaspin, but not serum omentin, in Wistar rats Int J Exp Pathol, 2017;0(0):. 2017 [PMID: 28444853] (Human)
• A Binia, C Vargas-Mar, M Ancira-Mor, LM Gosoniu, I Montoliu, E Gámez-Vald, DC Soria-Cont, A Angeles-Qu, R Gonzalez-A, S Fernández, D Martínez-C, B Hernández-, M Ramírez-So, C Pérez-Orte, Y Rodríguez-, I Castan, I Rubio-Alia, F Vadillo-Or, R Márquez-Ve, R Bojalil, JC López-Alva, P Valet, M Kussmann, I Silva-Zole, ME Tejero Improvement of cardiometabolic markers after fish oil intervention in young Mexican adults and the role of PPAR? L162V and PPAR?2 P12A J. Nutr. Biochem., 2017;43(0):98-106. 2017 [PMID: 28282585] (Human)
• H Lavi, M Assayag, A Schwartz, N Arish, ZG Fridlender, N Berkman The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis PLoS ONE, 2017;12(3):e0171945. 2017 [PMID: 28253271] (Human)
• Circulating periostin in relation to insulin resistance and nonalcoholic fatty liver disease among overweight and obese subjects Sci Rep, 2016;6(0):37886. 2016 [PMID: 27885258] (Human)
• Plasma selenium levels and nonalcoholic fatty liver disease in Chinese adults: a cross-sectional analysis Sci Rep, 2016;6(0):37288. 2016 [PMID: 27853246] (Human)
• Aldo Bonaventur Intraplaque Expression of C-Reactive Protein Predicts Cardiovascular Events in Patients with Severe Atherosclerotic Carotid Artery Stenosis Mediators Inflamm, 2016;2016(0):9153673. 2016 [PMID: 27738391] (Human)
• Silk-based blood stabilization for diagnostics Proc Natl Acad Sci USA, 2016;113(21):5892-7. 2016 [PMID: 27162330] (Human)
• Erdman L, D'Acremont V, Hayford K, Rajwans N, Kilowoko M, Kyungu E, Hongoa P, Alamo L, Streiner D, Genton B, Kain K Biomarkers of Host Response Predict Primary End-Point Radiological Pneumonia in Tanzanian Children with Clinical Pneumonia: A Prospective Cohort Study. PLoS ONE, 2015;10(9):e0137592. 2015 [PMID: 26366571] (Human)
• McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women. PLoS ONE, 2015;10(8):e0134619. 2015 [PMID: 26247200] (Human)
• Quon B, Ngan D, Wilcox P, Man S, Sin D Plasma sCD14 as a biomarker to predict pulmonary exacerbations in cystic fibrosis. PLoS ONE, 2014;9(2):e89341. 2014 [PMID: 24586701] (Human)
• Dogan , Meeshant, Shields , Bridget, Cutrona , Carolyn, Gao , Long, Gibbons , Frederic, Simons , Ronald, Monick , Martha, Brody , Gene H, Tan , Kai, Beach , Steven R, Philibert , Robert A The effect of smoking on DNA methylation of peripheral blood mononuclear cells from African American women. BMC Genomics, 2014;15(0):151. 2014 [PMID: 24559495] (Human)
• Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014;14(0):35. 2014 [PMID: 24444080] (Human)
• Wium C, Aasheim E, Ueland T, Michelsen A, Thorsby P, Larsen I, Torjesen P, Aukrust P, Birkeland K Differences in insulin sensitivity, lipid metabolism and inflammation between young adult Pakistani and Norwegian patients with type 2 diabetes: a cross sectional study. BMC Endocr Disord, 2013;13(0):49. 2013 [PMID: 24148878] (Human)
• Conroy AL, Liles WC, Molyneux ME Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi. PLoS ONE, 2011;6(12):e28540. 2011 [PMID: 22174834] (Human)
• Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study. PLoS ONE, 2011;6(2):e17440. 2011 [PMID: 21364762] (Human)
• Shyamsundar M, McKeown ST, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, Craig TR, Brown V, Thickett DR, Matthay MA, Taggart CC, Backman JT, Elborn JS, McAuley DF Simvastatin decreases lipopolysaccharide-induced pulmonary inflammation in healthy volunteers. Am. J. Respir. Crit. Care Med., 2009;179(12):1107-14. 2009 [PMID: 19324974] (Human)

Bioinformatics

• Gene Symbol: CRP