Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin]

Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin] [NB110-81701] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD29 (MEM-101A).Lane 1: lysate of human PBLLane 2: lysate of canine PBLLane 3: lysate of porcine PBL

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin] [NB110-81701] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) biotin, streptavidin-APC.

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin] [NB110-81701] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC.

Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin] [NB110-81701] - Staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.

• Reactivity: Hu, Po, Ca, Mu(-)
• Applications: WB, Flow, IP
• Clone: MEM-101A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: 1.0 mg/ml

Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin] Summary

• Additional Information: Clone MEM-101A was used by HLDA to establish CD designation.
• Immunogen: Raji Burkitt's lymphoma cell line (NM_002211.3).
• Marker: Stem Cell Marker
• Specificity: The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). HLDA VI; WS Code AS A048
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: Purity is > 95% (by SDS-PAGE).
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371
• Control:

  Integrin beta 1/CD29 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin beta 1/CD29 Antibody (MEM-101A) [Biotin]

• CD29 antigen
• CD29
• Fibronectin receptor subunit beta
• FNRBVLAB
• GPIIA
• Integrin beta 1
• integrin beta-1
• integrin VLA-4 beta subunit
• integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
• ITGB1
• MDF2
• MSK12
• very late activation protein, beta polypeptide
• VLA-4 subunit beta
• VLA-BETA

Background

CD29 (b1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 dibberent a chains (a1-a6, CD49a-f) determining the binding properties of b1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most b1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of b1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a b1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin beta 1/CD29 Antibody (NB110-81701)(3)

Publications using NB110-81701

• Leukocyte Typing VI, Kishimoto T et al (Eds). Garland Publishing Inc. 1997-01-01
• Planka L, Necas A, Srnec R et al. Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs Physiol Res 2009-01-01 [PMID: 19093735]
  
  Details:
  This citation used the Biotin version of this antibody.
• Simova S, Klima M, Cermak L, Sourkova V, Andera L. Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane Apoptosis 2008-03-01 [PMID: 18165900]
  
  Details:
  This citation used the FITC version of this antibody.

FAQs for Integrin beta 1/CD29 Antibody (NB110-81701). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
   • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Bioinformatics

• Gene Symbol: ITGB1