Integrin beta 1/CD29 Antibody (MEM-101A)


Western Blot: Integrin beta 1 Antibody (MEM-101A) [NB500-383] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody more

Product Details

Reactivity Hu, Po, CaSpecies Glossary
Applications WB, Flow, IP
1 mg/ml

Integrin beta 1/CD29 Antibody (MEM-101A) Summary

Raji Burkitt's lymphoma cell line Raji (NM_002211.3).
Stem Cell Marker
The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). HLDA VI; WS Code AS A048
Protein A purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS (pH 7.4)
15mM Sodium Azide
1 mg/ml
Protein A purified


  • Western Blot 2 ug/ml
  • Flow Cytometry 2 ug/ml
  • Immunoprecipitation 1:50
Application Notes
Purity is > 95% (by SDS-PAGE). Western Blot - Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel, 4% stacking gel). Recommended secondary antibody - anti mouse IgG/HRP, dilution 1:2000, 60 min on vertical incubator.
Read Publications using NB500-383.

Alternate Names for Integrin beta 1/CD29 Antibody (MEM-101A)

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta


CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 1/CD29 Antibody (NB500-383)(4)

Showing Publications 1 - 4 of 4.
Publications using NB500-383 Applications Species
Chen PW, Luo R, Jian X, Randazzo PA. The Arf6 GTPase-Activating Proteins ARAP2 and ACAP1 Define Distinct Endosomal Compartments that Regulate Integrin a5b1 Traffic. J Biol Chem 2014 Sep 15 [PMID:25225293]
Quach NL, Biressi S, Reichardt LF et al. Focal Adhesion Kinase Signaling Regulates the Expression of Caveolin 3 {beta}1 Integrin, Genes Essential for Normal Myoblast Fusion. Mol Biol Cell;20(14):3422-3435. 2009 [PMID:19458188]
Planka L, Necas A, Srnec R et al. Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs Physiol Res Jul 1 1905 12:00AM
Simova S, Klima M, Cermak L et al. Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane Apoptosis

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Integrin beta 1/CD29 Antibody (NB500-383). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB500-383AF405
Alexa Fluor (R) 488 Labeled NB500-383AF488
Alexa Fluor (R) 647 Labeled NB500-383AF647
Alexa Fluor (R) 700 Labeled NB500-383AF700
Allophycocyanin Labeled NB500-383APC
Biotin Labeled NB110-81701
DyLight 350 Labeled NB500-383UV
DyLight 405 Labeled NB500-383V
DyLight 405LS Labeled NB500-383V3
DyLight 488 Labeled NB500-383G
DyLight 680 Labeled NB500-383FR
DyLight 755 Labeled NB500-383IR
FITC Labeled NB500-384
PE Labeled NB500-383PE
PerCP Labeled NB500-383PCP

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits....  Read full blog post.

Contact Information

Product PDFs


Gene Symbol ITGB1

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