Integrin beta 1/CD29 Antibody (MEM-101A)

Images

 
Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody ...read more
Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC conjugated.

Product Details

Summary
Reactivity Hu, Po, CaSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clone
MEM-101A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Integrin beta 1/CD29 Antibody (MEM-101A) Summary

Immunogen
Raji Burkitt's lymphoma cell line Raji (NM_002211.3).
Marker
Stem Cell Marker
Specificity
The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). HLDA VI; WS Code AS A048
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 2 ug/ml
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Purity is > 95% (by SDS-PAGE). Western Blot - Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel, 4% stacking gel). Recommended secondary antibody - anti mouse IgG/HRP, dilution 1:2000, 60 min on vertical incubator.
Positive Control
Integrin beta 1/CD29 Lysate (NBL1-12069)
Publications
Read Publications using
NB500-383 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Integrin beta 1/CD29 Antibody (MEM-101A)

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-91258
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-77417
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
NBP2-45923
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-610
Species: Hu, Ca, GP
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP
796-IC
Species: Mu
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1008
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
NB500-328
Species: Hu
Applications: Flow, IP, CyTOF-ready
AF1320
Species: Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
BBA37
Species: Hu
Applications: AdBlk
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
AF2067
Species: Hu, Po, Ca
Applications: WB, Flow, IHC, CyTOF-ready, ICC
NB110-89474
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB500-383
Species: Hu, Po, Ca
Applications: WB, Flow, IP, CyTOF-ready

Publications for Integrin beta 1/CD29 Antibody (NB500-383)(7)

We have publications tested in 1 confirmed species: Canine.

We have publications tested in 1 application: FLOW.


Filter By Application
FLOW
(1)
All Applications
Filter By Species
Canine
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB500-383 Applications Species
Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine)

Details:
Used the PE form of this antibody.
FLOW Canine
Chen PW, Luo R, Jian X, Randazzo PA. The Arf6 GTPase-Activating Proteins ARAP2 and ACAP1 Define Distinct Endosomal Compartments that Regulate Integrin a5b1 Traffic. J Biol Chem 2014 Sep 15 [PMID: 25225293]

Details:
Using the PE conjugated version of NB500-383, catalog number NB500-383PE.
Quach NL, Biressi S, Reichardt LF et al. Focal Adhesion Kinase Signaling Regulates the Expression of Caveolin 3 {beta}1 Integrin, Genes Essential for Normal Myoblast Fusion. Mol Biol Cell;20(14):3422-3435. 2009 [PMID: 19458188]
Planka L, Necas A, Srnec R et al. Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs Physiol Res 2009 [PMID: 19093735]

Details:
This citation used the Biotin version of this antibody.
Simova S, Klima M, Cermak L, Sourkova V, Andera L. Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane Apoptosis 2008 Mar [PMID: 18165900]

Details:
This citation used the FITC version of this antibody.
Planka L, Necas A, Srnec R et al. Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs Physiol Res Jul 1 1905

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NB110-81701) and the FITC conjugated form of this antibody (Cat# NB500-384).
Simova S, Klima M, Cermak L et al. Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane Apoptosis

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NB110-81701) and the FITC conjugated form of this antibody (Cat# NB500-384).

Reviews for Integrin beta 1/CD29 Antibody (NB500-383) (0)

There are no reviews for Integrin beta 1/CD29 Antibody (NB500-383). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Integrin beta 1/CD29 Antibody (NB500-383). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-383AF405
Alexa Fluor 488 Labeled NB500-383AF488
Alexa Fluor 647 Labeled NB500-383AF647
Alexa Fluor 700 Labeled NB500-383AF700
Allophycocyanin Labeled NB500-383APC
Biotin Labeled NB110-81701
DyLight 350 Labeled NB500-383UV
DyLight 405 Labeled NB500-383V
DyLight 488 Labeled NB500-383G
DyLight 680 Labeled NB500-383FR
DyLight 755 Labeled NB500-383IR
FITC Labeled NB500-384
PE Labeled NB500-383PE
PerCP Labeled NB500-383PCP

Additional Integrin beta 1/CD29 Products

Bioinformatics Tool for Integrin beta 1/CD29 Antibody (NB500-383)

Discover related pathways, diseases and genes to Integrin beta 1/CD29 Antibody (NB500-383). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Integrin beta 1/CD29 Antibody (NB500-383)

Discover more about diseases related to Integrin beta 1/CD29 Antibody (NB500-383).
 

Pathways for Integrin beta 1/CD29 Antibody (NB500-383)

View related products by pathway.

PTMs for Integrin beta 1/CD29 Antibody (NB500-383)

Learn more about PTMs related to Integrin beta 1/CD29 Antibody (NB500-383).
 

Research Areas for Integrin beta 1/CD29 Antibody (NB500-383)

Find related products by research area.

Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits....  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Integrin beta 1/CD29 Antibody (MEM-101A) and receive a gift card or discount.

Bioinformatics

Gene Symbol ITGB1
Entrez