Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

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Western Blot: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-Integrin beta 1 Antibody ...read more
Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Staining of human peripheral blood with anti-human CD29 (MEM-101A) PE.
Flow Cytometry: Integrin beta 1/CD29 Antibody (MEM-101A) [NB500-383] - Surface staining of human peripheral blood with anti-human CD29 (MEM-101A) APC conjugated.

Product Details

Summary
Reactivity Hu, Po, Ca, Mu(-)Species Glossary
Applications WB, Flow, IP, CyTOF-ready
Clone
MEM-101A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free Summary

Additional Information
Clone MEM-101A was used by HLDA to establish CD designation.
Immunogen
Raji Burkitt's lymphoma cell line (NM_002211.3).
Marker
Stem Cell Marker
Specificity
The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). HLDA VI; WS Code AS A048
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 2 ug/ml
Application Notes
Purity is > 95% (by SDS-PAGE). Western Blot - Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS Application note: Non-reducing conditions. SDS-PAGE (6% separating gel, 4% stacking gel). Recommended secondary antibody - anti mouse IgG/HRP, dilution 1:2000, 60 min on vertical incubator. This antibody is CyTOF ready.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Integrin beta 1/CD29 Overexpression Lysate
Publications
Read Publications using
NB500-383 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Integrin beta 1/CD29 Antibody (MEM-101A) - BSA Free

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

CD29 (beta1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CD49a-f) determining the binding properties of beta1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most beta1 integrins correlates with acquiring multidrug resistance of tumour cells during selection in presence of antitumour drug. In platelets, translocation of intracellular pool of beta1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a beta1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 1/CD29 Antibody (NB500-383)(8)

We have publications tested in 2 confirmed species: Canine, Porcine.

We have publications tested in 2 applications: FLOW, ICC/IF.


Filter By Application
FLOW
(1)
ICC/IF
(1)
All Applications
Filter By Species
Canine
(1)
Porcine
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB500-383 Applications Species
Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine)

Details:
Used the PE form of this antibody.
FLOW Canine
Chen PW, Luo R, Jian X, Randazzo PA. The Arf6 GTPase-Activating Proteins ARAP2 and ACAP1 Define Distinct Endosomal Compartments that Regulate Integrin a5b1 Traffic. J Biol Chem 2014-09-15 [PMID: 25225293]

Details:
Using the PE conjugated version of NB500-383, catalog number NB500-383PE.
Planka L, Necas A, Srnec R et al. Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs Physiol Res 2009-01-01 [PMID: 19093735]

Details:
This citation used the Biotin version of this antibody.
Simova S, Klima M, Cermak L, Sourkova V, Andera L. Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane Apoptosis 2008-03-01 [PMID: 18165900]

Details:
This citation used the FITC version of this antibody.
Kishimoto T et al. Leukocyte Typing VI. Garland Publishing Inc. 1997-01-01
Kell MJ, Ang SF, Pigati L et al. AP-1B facilitates endocytosis during cell migration bioRxiv 2019-10-25 (ICC/IF, Porcine) ICC/IF Porcine
Kell MJ, Ang SF, Pigati L et al. Novel function for AP-1B during cell migration Mol. Biol. Cell 2020-08-20 [PMID: 32816642]
Quach NL, Biressi S, Reichardt LF et al. Focal Adhesion Kinase Signaling Regulates the Expression of Caveolin 3 {beta}1 Integrin, Genes Essential for Normal Myoblast Fusion. Mol Biol Cell;20(14):3422-3435. 2009-01-01 [PMID: 19458188]

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FAQs for Integrin beta 1/CD29 Antibody (NB500-383). (Showing 1 - 1 of 1 FAQs).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

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Blogs on Integrin beta 1/CD29.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits. ...  Read full blog post.

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Bioinformatics

Gene Symbol ITGB1