CD90/Thy1 Antibody (OX-7)

Images

 
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus with MOUSE ANTI RAT CD90 (THY 1.1).
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus cell.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IP
Clone
OX-7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD90/Thy1 Antibody (OX-7) Summary

Immunogen
Rat Thy 1 antigen
Marker
Mesenchymal Cell Marker
Specificity
CD90
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
THY1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Publications
Read Publications using
NB100-65543 in the following applications:

Reactivity Notes

Reacts with: Rabbit, Mouse, Guinea Pig. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD90/Thy1 Antibody (OX-7)

  • CD90 antigen
  • CD90
  • CDw90
  • FLJ33325
  • Thy-1 antigen
  • Thy-1 cell surface antigen
  • thy-1 membrane glycoprotein
  • Thy-1 T-cell antigen
  • Thy1

Background

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1. Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of MRC OX-7 for rat Thy1 is 3 x 10^9m^1 and for mouse Thy1.1 is 3 x 10^8m-^1(1). Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (5).This clone has been reported to induce glomerular nephritis in Wistar rats (8).This product is routinely tested in flow cytometry on rat thymocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD90/Thy1 Antibody (NB100-65543)(8)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: FLOW, ICC/IF.


Filter By Application
FLOW
(3)
ICC/IF
(1)
All Applications
Filter By Species
Rat
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-65543 Applications Species
Hoseini SJ, Ghazavi H, Forouzanfar F et al. Fibroblast Growth Factor 1-Transfected Adipose-Derived Mesenchymal Stem Cells Promote Angiogenic Proliferation. DNA Cell Biol. Mar 10 2017 [PMID: 28281780]
Homayounfar N, Verma P, Nosrat A et al. Isolation, Characterization, and Differentiation of Dental Pulp Stem Cells in Ferrets. J Endod. 2016 Mar [PMID: 26794343] (ICC/IF) ICC/IF
Biermann J, Boyle J, Pielen A et al. Histone deacetylase inhibitors sodium butyrate and valproic acid delay spontaneous cell death in purified rat retinal ganglion cells. Mol Vis. 2011 Feb. [PMID: 21311741]
Ohashi N, Urushihara M, Satou R et al. Glomerular angiotensinogen is induced in mesangial cells in diabetic rats via reactive oxygen species--ERK/JNK pathways. Hypertens Res. 2010 Nov. [PMID: 20686488]
Stevenson KS, McGlynn L, Hodge M et al. Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 2009 Dec. [PMID: 19326970]
Khaldoyanidi S, Schnabel D, Fohr N, Zoller M. Functional activity of CD44 isoforms in haemopoiesis of the rat. Br J Haematol. [PMID: 9012685] (FLOW, Rat) FLOW Rat
Crook K, Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. [PMID: 8924759] (FLOW, Rat) FLOW Rat
Lazo PA, Klein-Szanto AJ, Tsichlis PN. T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications. J Virol. [PMID: 2196385] (FLOW, Rat) FLOW Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD90/Thy1 Antibody (NB100-65543). (Showing 1 - 2 of 2 FAQs).

  1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.
  2. Can I use Cd90/Thy1antibody (OX-7) NB100-65543 for mouse tissue to do Western Blot? What additional optimization is required?
    • Our datasheet says that this antibody can be used in these applications: WB, Flow, IHC-Fr, and IP with the samples from mouse, rat, guinea pig, and rabbit. If using this antibody in above applications with the sample(s) from the species, we guarantee it will produce the positive results, or we will refund or replace the antibody free. [When using in IHC-Fr, the mouse-on-mouse (http://vectorlabs.com/mouse-on-mouse-m-o-m-blocking-reagent.html) will be required]. It does not have any special instructions for using this antibody in WB, and therefore our general WB protocol can apply: Protocol. Our recommendation for dilution in WB: 1:100-1:2000. Use the above protocol/dilution to start a small test first. After getting the positive result(s), you could adjust the dilution factor of the antibody as you are repeating/duplicating the experiment, to obtain the best image(s).

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-65543AF405
Alexa Fluor 488 Labeled NB100-65543AF488
Alexa Fluor 647 Labeled NB100-65543AF647
Alexa Fluor 700 Labeled NB100-65543AF700
Allophycocyanin Labeled NB100-65543APC
DyLight 350 Labeled NB100-65543UV
DyLight 405 Labeled NB100-65543V
DyLight 488 Labeled NB100-65543G
DyLight 680 Labeled NB100-65543FR
DyLight 755 Labeled NB100-65543IR
PE Labeled NB100-65543PE
PerCP Labeled NB100-65543PCP

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Bioinformatics Tool for CD90/Thy1 Antibody (NB100-65543)

Discover related pathways, diseases and genes to CD90/Thy1 Antibody (NB100-65543). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD90/Thy1.

CD90 (Cluster of differentiation 90)
CD90 is a 25-35kD glycosylphosphatidylinositol (GPI)-linked glycoprotein receptor of the immunoglobulin (Ig) superfamily. It is found on murine T-cells, thymocytes, neuronal cells, granulocytic lineage-derived cells, hematopoietic stem cells, fibrobla...  Read full blog post.

How New Oval Stem Cell Marker Antibodies Will Benefit Hepatic Research
A large number of antibody suppliers supply conjugated and non-conjugated marker antibodies, targeted at specific stem cell populations. Until recently the number of oval stem cell markers was extremely limited. However, we at Novus Biologicals have s...  Read full blog post.

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Bioinformatics

Gene Symbol THY1
Entrez
Uniprot