Reacts with: Rabbit, Mouse, Guinea Pig. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate buffered saline
0.09% Sodium Azide
Protein A purified
Alternate Names for CD90/Thy1 Antibody (OX-7)
Thy-1 cell surface antigen
thy-1 membrane glycoprotein
Thy-1 T-cell antigen
CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1. Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of MRC OX-7 for rat Thy1 is 3 x 10^9m^1 and for mouse Thy1.1 is 3 x 10^8m-^1(1). Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (5).This clone has been reported to induce glomerular nephritis in Wistar rats (8).This product is routinely tested in flow cytometry on rat thymocytes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CD90/Thy1 Antibody (NB100-65543). (Showing 1 - 2 of 2 FAQs).
We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.
Can I use Cd90/Thy1antibody (OX-7) NB100-65543 for mouse tissue to do Western Blot? What additional optimization is required?
Our datasheet says that this antibody can be used in these applications: WB, Flow, IHC-Fr, and IP with the samples from mouse, rat, guinea pig, and rabbit. If using this antibody in above applications with the sample(s) from the species, we guarantee it will produce the positive results, or we will refund or replace the antibody free. [When using in IHC-Fr, the mouse-on-mouse (http://vectorlabs.com/mouse-on-mouse-m-o-m-blocking-reagent.html) will be required]. It does not have any special instructions for using this antibody in WB, and therefore our general WB protocol can apply: Protocol. Our recommendation for dilution in WB: 1:100-1:2000. Use the above protocol/dilution to start a small test first. After getting the positive result(s), you could adjust the dilution factor of the antibody as you are repeating/duplicating the experiment, to obtain the best image(s).
CD90 (Cluster of differentiation 90) CD90 is a 25-35kD glycosylphosphatidylinositol (GPI)-linked glycoprotein receptor of the immunoglobulin (Ig) superfamily. It is found on murine T-cells, thymocytes, neuronal cells, granulocytic lineage-derived cells, hematopoietic stem cells, fibrobla... Read full blog post.
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