CD90/Thy1 Antibody (OX-7) - BSA Free

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Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus with MOUSE ANTI RAT CD90 (THY 1.1).
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymocytes.
Flow Cytometry: CD90/Thy1 Antibody (OX-7) [NB100-65543] - Staining of rat thymus cell.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IP
Clone
OX-7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD90/Thy1 Antibody (OX-7) - BSA Free Summary

Immunogen
Rat Thy 1 antigen
Marker
Mesenchymal Cell Marker
Specificity
CD90
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
THY1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Publications
Read Publications using
NB100-65543 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:28928774).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD90/Thy1 Antibody (OX-7) - BSA Free

  • CD90 antigen
  • CD90
  • CDw90
  • FLJ33325
  • Thy-1 antigen
  • Thy-1 cell surface antigen
  • thy-1 membrane glycoprotein
  • Thy-1 T-cell antigen
  • Thy1

Background

CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1. Thy1.1, expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of OX-7 for rat Thy1 is 3 x 10^9m^1 and for mouse Thy1.1 is 3 x 10^8m-^1(1). Clone OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (5).This clone has been reported to induce glomerular nephritis in Wistar rats (8).This product is routinely tested in flow cytometry on rat thymocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD90/Thy1 Antibody (NB100-65543)(12)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: FLOW, Flow, ICC/IF, IHC.


Filter By Application
FLOW
(3)
Flow
(2)
ICC/IF
(2)
IHC
(1)
All Applications
Filter By Species
Mouse
(1)
Rat
(5)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-65543 Applications Species
Tigges J, Eggerbauer F, Worek F Et al. Optimization of long-term cold storage of rat precision cut lung slices (PCLS) with a tissue preservation solution American journal of physiology. Lung cellular and molecular physiology Oct 13 2021 [PMID: 34643087] (Flow, Rat) Flow Rat
Zhang J, Wang JH. Maintenance of Tendon Stem/Progenitor Cells in Culture. Methods Mol. Biol. Sep 10 2018 [PMID: 30196413] (ICC/IF, Rat) ICC/IF Rat
Hassanzadeh H, Matin MM, Naderi-Meshkin H et al. Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications Cell Tissue Bank 2018 Aug 13 [PMID: 30105667] (Flow) Flow
Li F, Ma Z et al. Y-box Protein-1 Regulates the Expression of Collagen I in Hepatic Progenitor Cells via PDGFR-beta/ERK/p90RSK Signalling. Stem Cells Int Sep 21 2017 [PMID: 28928774] (IHC, Mouse) IHC Mouse
Hoseini SJ, Ghazavi H, Forouzanfar F et al. Fibroblast Growth Factor 1-Transfected Adipose-Derived Mesenchymal Stem Cells Promote Angiogenic Proliferation. DNA Cell Biol. Mar 10 2017 [PMID: 28281780]
Homayounfar N, Verma P, Nosrat A et al. Isolation, Characterization, and Differentiation of Dental Pulp Stem Cells in Ferrets. J Endod. 2016 Mar [PMID: 26794343] (ICC/IF) ICC/IF
Biermann J, Boyle J, Pielen A et al. Histone deacetylase inhibitors sodium butyrate and valproic acid delay spontaneous cell death in purified rat retinal ganglion cells. Mol Vis. 2011 Feb. [PMID: 21311741]
Ohashi N, Urushihara M, Satou R et al. Glomerular angiotensinogen is induced in mesangial cells in diabetic rats via reactive oxygen species--ERK/JNK pathways. Hypertens Res. 2010 Nov. [PMID: 20686488]
Stevenson KS, McGlynn L, Hodge M et al. Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells Dev. 2009 Dec. [PMID: 19326970]
Khaldoyanidi S, Schnabel D, Fohr N, Zoller M. Functional activity of CD44 isoforms in haemopoiesis of the rat. Br J Haematol. [PMID: 9012685] (FLOW, Rat) FLOW Rat
Show All 12 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD90/Thy1 Antibody (NB100-65543). (Showing 1 - 2 of 2 FAQs).

  1. We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of theses <a href="http://www.novusbio.com/productsearch/CD90%252FThy1#fq=common_name%3A%22CD90%2FThy1%22&fq=species%3A%22Canine%22" target="_self">CD90 antibodies</a>, as these have all been validated for use with dog/canine samples.
  2. Can I use Cd90/Thy1antibody (OX-7) NB100-65543 for mouse tissue to do Western Blot? What additional optimization is required?
    • Our datasheet says that this antibody can be used in these applications: WB, Flow, IHC-Fr, and IP with the samples from mouse, rat, guinea pig, and rabbit. If using this antibody in above applications with the sample(s) from the species, we guarantee it will produce the positive results, or we will refund or replace the antibody free. [When using in IHC-Fr, the mouse-on-mouse (http://vectorlabs.com/mouse-on-mouse-m-o-m-blocking-reagent.html) will be required]. It does not have any special instructions for using this antibody in WB, and therefore our general WB protocol can apply: Protocol. Our recommendation for dilution in WB: 1:100-1:2000. Use the above protocol/dilution to start a small test first. After getting the positive result(s), you could adjust the dilution factor of the antibody as you are repeating/duplicating the experiment, to obtain the best image(s).

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CD90/Thy1 Antibody (NB100-65543)

Discover related pathways, diseases and genes to CD90/Thy1 Antibody (NB100-65543). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD90/Thy1.

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CD90 is a 25-35kD glycosylphosphatidylinositol (GPI)-linked glycoprotein receptor of the immunoglobulin (Ig) superfamily. It is found on murine T-cells, thymocytes, neuronal cells, granulocytic lineage-derived cells, hematopoietic stem cells, fibrobla...  Read full blog post.

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Bioinformatics

Gene Symbol THY1
Entrez
Uniprot